Diversification of Molecular Structures by Merger of Biosynthetic Pathways
| Project/Area Number |
20241049
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
EBIZUKA Yutaka The University of Tokyo, 生薬部, 客員研究員 (90107384)
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| Co-Investigator(Kenkyū-buntansha) |
SHIBUYA Masaaki 新潟薬科大学, 薬学部, 教授 (50170923)
KUSHIRO Tetsuo 明治大学, 農学部, 准教授 (80373299)
ABE Ikurou 東京大学, 大学院・薬学系研究科, 教授 (40305496)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥30,290,000 (Direct Cost: ¥23,300,000、Indirect Cost: ¥6,990,000)
Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2008: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
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| Keywords | 生合成 / メロテルペノイド / 遺伝子 / 糸状菌 / 遺伝子クラスター / ピリピロペン / テレトニン / ポリケタイド合成酵素 / テルペン環化酵素 / 基質特異性 / プレニル基転移酵素 / Aspergillus fumigatus / ニコチン酸 |
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| Research Abstract |
By searching for gene-clusters that contain both putative PKS and PT in public gene database of filamentous fungi, two meroterpenoid gene clusters have been found and identified for the first time. All PKSs, PTs and CYCs found in this study show novel reactivity and specificity, and thus are expected to contribute for construction of structural diversity in a combinatorial fashion. Heterologous expression of the enzyme genes reconstituted a meroterpenoid biosynthesis. Further, a substrate directed biosynthesis afforded a non-natural meroterpenoid.
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Report
(4 results)
Research Products
(45 results)
