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Construction of Drug-Discovering System by using Multiple Fluorescent Labels on Peptide Library

Research Project

Project/Area Number 20241054
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Living organism molecular science
Research InstitutionOkayama University

Principal Investigator

SISIDO Masahiko  Okayama University, 大学院・自然科学研究科, 特命教授(研究) (60026268)

Co-Investigator(Kenkyū-buntansha) KITAMATSU Mizuki  岡山大学, 大学院・自然科学研究科, 助教 (60379716)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥44,590,000 (Direct Cost: ¥34,300,000、Indirect Cost: ¥10,290,000)
Fiscal Year 2010: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
Fiscal Year 2009: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
Fiscal Year 2008: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
Keywords蛍光性アミノ酸 / ペプチドライブラリー / がん細胞 / インビトロスクリーニング / ステルス標識 / 2次元蛍光スペクトル / ペプチド / スクリーニング / 蛍光標識 / 細胞識別
Research Abstract

A lot of researchers have been trying to discover peptides that bind onto specific cancer cells, however, without success. A major reason for the unsucceesful attempts may be that the discovery processes have been done under conditions far from the conditions inside the living body. The purpose of this project is to establish a novel screening process by using multiple fluorescent labels on peptide library. Putting a solution of the peptide library onto cancer cells and identifying those bound onto the cells with fluorescence spectroscopy, we found several cancer cell specific octapeptides.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (46 results)

All 2011 2010 2009 2008

All Journal Article (26 results) (of which Peer Reviewed: 23 results) Presentation (16 results) Book (2 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Antisense effects of pyrrolidine-based oxy-peptide nucleic acids in Escherichia coli2011

    • Author(s)
      M.Kitamatsu, S.Kurami, T.Ohtsuki, M.Sisido
    • Journal Title

      Bioorganic & Medicinal Chemistry Letters

      Volume: 21 Pages: 225-227

    • NAID

      120003353368

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantitative Screening of EGF Receptor-Binding Peptide by Using a Peptide Library with Multiple Fluorescent Amino Acids as Fluorescent Tags2010

    • Author(s)
      M.Kittamatsu, T.Yamamoto, M.Futami, M.Sisido
    • Journal Title

      Bioorg.Med.Chem.Lett. 20

      Pages: 5976-5978

    • NAID

      120003386547

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prevention of Absorption of Fluorescent Amino Acids to Gels by Using a Peptide Containing Dendritic Amino Acids2010

    • Author(s)
      M.Kitamatsu, K.Akagi, M.Sisido
    • Journal Title

      Chem.Lett. 39

      Pages: 1240-1241

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Introduction of a highly photodurable and common-laser excitable fluorescent amino acid into a peptide as a FRET acceptor for protease cleavage detection2010

    • Author(s)
      M.Taki, Y.Yamazaki, Y.Suzuki, M.Sisido
    • Journal Title

      Chem.Lett. 39

      Pages: 818-819

    • NAID

      10027183581

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Novel Method for Screeninig Peptides that Bind to Proteins by Using Multiple Fluorescent Amino Acides as Fluorescent Tags2010

    • Author(s)
      M.Kitamatsu, M.Futami, M.Sisido
    • Journal Title

      Chem.Comm. 46

      Pages: 761-763

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Use of EF-Tu mutants for determining and improving aminoacylation efficiency and for purifying aminoacyl tRNAs with non-natural amino acids2010

    • Author(s)
      T.Ohtsuki, H.Yamamoto, Y.Doi, M.Sisido
    • Journal Title

      Journal of Biochemistry

      Volume: 148 Pages: 239-246

    • NAID

      10027877901

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lactobacillus-mediated RNA interference in nematode2010

    • Author(s)
      A.Kuwahara, M.Arita, A.Kushiro, Y.Sakube, M.Sisido, T.Ohtsuki
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 109 Pages: 189-192

    • NAID

      110007573252

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of pyrrolidine-based oxay-peptide nucleic acids carrying four types of nucleobases and their transport into cytoplasm2010

    • Author(s)
      M.Kitamatsu, A.Takahashi, T.Ohtsuki M.Sisido
    • Journal Title

      Tetrahedron

      Volume: 66 Pages: 9659-9666

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prevention of Absorption of Fluorescent Amino Acids to Gels by Using a Peptide Containing Dendritic Amion Acids2010

    • Author(s)
      M.Kitamatsu, K.Akagi, M.Sisido
    • Journal Title

      Chem Lett

      Volume: 39 Pages: 1240-1241

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantitative Screening of EGF Receptor-Binding Peptide by Using a Peptide Library with Multiple Fluorescent Amino Acids as Fluorescent Tags2010

    • Author(s)
      M.Kitamatsu, T.Yamamoto, M.Futami, M.Sisido
    • Journal Title

      Bioorg.Med.Chem.Lett

      Volume: 20 Pages: 5976-5978

    • NAID

      120003386547

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Novel Method for Screeninig Peptides that Bind to Proteins by Using Multiple Fluorescent Amino Acides as Fluorescent Tags2010

    • Author(s)
      M.Kitamatsu, M.Futami, M.Sisido
    • Journal Title

      Chem.Commun

      Volume: 46 Pages: 761-763

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Introduction of a highly photodurable and common-laser excitable fluorescent amino acid into a peptide as a FRET acceptor for protease cleavage detection2010

    • Author(s)
      M.Taki, Y.Yamazaki, Y.Suzuki, M.Sisido
    • Journal Title

      Chem Lett

      Volume: 39 Pages: 818-819

    • NAID

      10027183581

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Position specific incorporation of nonnatural amino acids into proteins in vivo2010

    • Author(s)
      S.Neki, T.Ohtsuki, M.Sisido
    • Journal Title

      Animal Cell Technology : Basic & Applied Aspects (Eds.By M.Kamihira, Y.Katakura and A.Ito)

      Volume: 16 Pages: 435-440

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Improvement of CPP-RBD for siRNA delivery2010

    • Author(s)
      R.Kuwabara, T.Endoh, M.Sisido, T.Ohtsuki
    • Journal Title

      Animal Cell Technology : Basic & Applied Aspects (Eds.By M.Kamihira. Y.Katakura and A.Ito)

      Volume: 16 Pages: 191-196

    • Related Report
      2010 Annual Research Report
  • [Journal Article] N-Terminal Specific Point-Immobilization of Active Proteins by the One-Pot NEXT-A Method2009

    • Author(s)
      Kawakami, M.Ikeuchi, J.Hirabayashi, M.Sisido, M.Taki
    • Journal Title

      ChemBioChem 10(15)

      Pages: 2460-2464

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection of Bioactive Small Molecules by Fluorescent Resonance Energy Transfer(FRET)in RNA-Protein Conjugate2009

    • Author(s)
      T.Endoh, R.Shintani, M.Mie, E.Kobatake, T.Ohtsuki, M.Sisido
    • Journal Title

      Bioconjugate Chemistry 20

      Pages: 2242-2246

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spatial regulation of specific gene expression through photoactivation of RNAi2009

    • Author(s)
      T.Endoh, M.Sisido, T.Ohtsuki
    • Journal Title

      Journal of Controlled Release 137

      Pages: 241-245

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A protease inhibitor discovery method using fluorescence correlation spectroscopy with position-specific labeled protein substrates2009

    • Author(s)
      H.Nakata, T.Ohtsuki, M.Sisido
    • Journal Title

      Anal. Biochem. 390

      Pages: 121-125

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Carrier PNA for shRNA delivery into cells2009

    • Author(s)
      M.Kitamatsu, T.Kubo, R.Matsuzaki, T.Endoh, T.Ohtsuki, M.Sisido
    • Journal Title

      Bioorganic & Medicinal Chemistry Letters 19

      Pages: 3410-3412

    • NAID

      120003386546

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PNA arrays for miRNA detection2009

    • Author(s)
      T.Endoh, M.Kitamatsu, M.Sisido, T.Ohtsuki
    • Journal Title

      Chem. Lett 38

      Pages: 438-439

    • NAID

      10025121754

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] N-terminal specific point-immobilization of active proteins via the one-pot NEXT-A method2009

    • Author(s)
      K.Ebisu, H.Tateno, H.Kuroira, K.Kawakami, M.Ikeuchi, J.Hirabayashi, M.Sisido, M.Taki
    • Journal Title

      ChemBioChem 10

      Pages: 2460-2464

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2009

    • Author(s)
      T.Ohtsuki, M.Sisido
    • Journal Title

      The central dogma : from DNA to RNA, and to protein. In "Automation in Proteomics and Genomics"(Wiley)

      Pages: 3-20

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Chemoenzymatic transfer of fluorescent nonnatural amino acids to the N-terminus of aprotein/peptide2008

    • Author(s)
      M.Taki, H.Kuroiwa, M.Sisido
    • Journal Title

      ChemBioChem. 9(5)

      Pages: 719-722

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of small RNAs using biotinylated PNAs.2008

    • Author(s)
      T. Ohtsuki. T. Fujimoto, M. Kamimukai, C. Kumano, M. Kitamatsu, M. Sisido
    • Journal Title

      Journal of Biochemistry 144

      Pages: 415-418

    • NAID

      10024912545

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cellular siRNA delivery mediated by a cell-permeant RNA-binding protein and photoinduced RNA interference.2008

    • Author(s)
      T. Endoh, M. Sisido, T. Ohtsuki
    • Journal Title

      Bioconjugate Chemistry 19

      Pages: 1017-1024

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chemoenzymatic Transfer of Fluorescent Non-natural Amino Acids to the N Ternimus of a Protein/Peptide2008

    • Author(s)
      M. Taki. H. Kuroiwa. M. Sisido
    • Journal Title

      ChemBioChem. 9

      Pages: 719-722

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] N-terminal specific 18F-labeling of peptides and proteins2010

    • Author(s)
      H.Kuroiwa, M.Sisido, M.Taki
    • Organizer
      The International Chemical Congress of Pacific Basin Societies
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      2010-12-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] Synthesis of cyclic peptides using the NEXT-A reaction2010

    • Author(s)
      M.Taki, T.Hamamoto, M.Sisido
    • Organizer
      The International Chemical Congress of Pacific Basin Societies
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      2010-12-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] Multi-labeled fluorescent peptide library for in situ screening against cancer cells2010

    • Author(s)
      M.Sisido, T.Fukuda
    • Organizer
      The 5th International Peptide Symposium
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2010-12-12
    • Related Report
      2010 Final Research Report
  • [Presentation] Introduction of positron emission tomography(PET) probes at the N-terminus of peptide/protein by the NEXT-Areaction2010

    • Author(s)
      M.Taki, H.Kuroiwa, M.Sisido
    • Organizer
      The 5th International Peptide Symposium
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2010-12-12
    • Related Report
      2010 Final Research Report
  • [Presentation] Multi-labeled fluorescent peptide library for in situ screening against cancer cells2010

    • Author(s)
      M.Sisido, T.Fukuda
    • Organizer
      The 5th International Peptide Symposium
    • Place of Presentation
      Kyoto, Japan(Invited)
    • Year and Date
      2010-12-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] Introduction of positron emission tomography(PET) probes at the N-terminus of peptide/protein by the NEXT-A reaction2010

    • Author(s)
      M.Taki, H.Kuroiwa, M.Sisido
    • Organizer
      The 5th International Peptide Symposium
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2010-12-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] In vitro and in vivo peptide screening from peptide libraries labeled with multi-fluorohores2010

    • Author(s)
      M.Sisido, T.Fukuda
    • Organizer
      The 13th Akabori Conference
    • Place of Presentation
      Leipzig, Germany.
    • Year and Date
      2010-09-13
    • Related Report
      2010 Final Research Report
  • [Presentation] In vitro and in vivo peptide screening from peptide libraries labeled with multi-fluorophores2010

    • Author(s)
      M.Sisido, T.Fukuda
    • Organizer
      The 13th Akabori Conference, 1010
    • Place of Presentation
      Leipzig, Germany(Invited)
    • Year and Date
      2010-09-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] A Method for Screening Peptides Bound to EGFR by Using Multiple Fluorescent Amino Acids as Fluorescent Tags2010

    • Author(s)
      M.Kitamatsu, T.Yamamoto, M.Sisido
    • Organizer
      The 31st European Peptide Symposium
    • Place of Presentation
      Copenhagen, Denmark.
    • Year and Date
      2010-09-05
    • Related Report
      2010 Final Research Report
  • [Presentation] A Method for Screening Peptides Bound to EGFR by Using Multiple Fluorescent Amino Acids as Fluorescent Tags2010

    • Author(s)
      M.Kitamatsu, T.Yamamoto, M.Sisido
    • Organizer
      The 31st European Peptide Symposium
    • Place of Presentation
      Copenhagen, Denmark
    • Year and Date
      2010-09-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] A Search for Peptides Binding to Assembly of Amyloid-Beta by Novel Fluorescence-Screening2009

    • Author(s)
      T.MORIKAWA, M.KITAMATSU, M.SISIDO
    • Organizer
      第46回ペプチド討論会
    • Place of Presentation
      北九州国際会議場(福岡)
    • Related Report
      2009 Annual Research Report
  • [Presentation] A Search for Peptides Binding to EGFR by Novel Fluorescence-Screening2009

    • Author(s)
      T.YAMAMOTO, M.KITAMATSU, M.SISIDO
    • Organizer
      第46回ペプチド討論会
    • Place of Presentation
      北九州国際会議場(福岡)
    • Related Report
      2009 Annual Research Report
  • [Presentation] N-TERMINAL SPECIFIC POINT-IMMOBILIZATION OF PEPTIDE/PROTEIN BY THE ONE-POT NEXT-A REACTION2009

    • Author(s)
      M.Taki, K.Ebisu M.Sisido
    • Organizer
      第46回ペプチド討論会
    • Place of Presentation
      北九州国際会議場(福岡)
    • Related Report
      2009 Annual Research Report
  • [Presentation] CLIP-RNAi photo induced RNAi strategy.2008

    • Author(s)
      T. Endoh, M. Sisido, T. Ohtsuki
    • Organizer
      3rd International Workshop on Approaches to Single-Cell Analysis
    • Place of Presentation
      Zurich (Switzerland)
    • Year and Date
      2008-09-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] Construction of RNA-protein Hybrid for Bioimaging of Low-molecular Compounds.2008

    • Author(s)
      T. Endoh, R. Shintani, M. Mie. E. Kobatake, T. Ohtsuki. M.
    • Organizer
      3rd International Workshop on Approaches to Single-Cell Analysis
    • Place of Presentation
      Zurich (Switzerland)
    • Year and Date
      2008-09-11
    • Related Report
      2008 Annual Research Report
  • [Presentation] Multi-component Fluorescence labeling method for Efficient Positional Screening of Peptide Library2008

    • Author(s)
      M. Kitamatsu, H. Kuroiwa. D. Sakata. S. Neki. S. Yagi, T.
    • Organizer
      30th European Peptide Symposium
    • Place of Presentation
      Helsinki
    • Year and Date
      2008-08-31
    • Related Report
      2008 Annual Research Report
  • [Book] 酵素利用技術大系、第4章「L/F-転移酵素による機能性非天然アミノ酸の蛋白質N末端への導入」pp.497-5042010

    • Author(s)
      瀧真清、宍戸昌彦
    • Total Pages
      1064
    • Publisher
      NTS社
    • Related Report
      2010 Annual Research Report
  • [Book] 化学の指針シリーズ「生物有機化学-ケミカルバイオロジーへの展開-」2008

    • Author(s)
      宍戸昌彦, 大槻高史
    • Total Pages
      204
    • Publisher
      裳華房
    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] 環状ペプチドを含む結合体およびその作製方法2009

    • Inventor(s)
      瀧真清、宍戸昌彦
    • Industrial Property Rights Holder
      岡山大学
    • Industrial Property Number
      2009-195254
    • Filing Date
      2009-08-26
    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] 目的タンパク質または目的ペプチドにアミノ酸を導入する方法2008

    • Inventor(s)
      瀧 真清, 宍戸昌彦
    • Industrial Property Rights Holder
      岡山大学
    • Industrial Property Number
      2008-181303
    • Filing Date
      2008-07-11
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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