• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Eco-genome study of adaptive genes in Primula sieboldii

Research Project

Project/Area Number 20241055
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Resource conservation science
Research InstitutionUniversity of Tsukuba

Principal Investigator

OHSAWA Ryo  筑波大学, 大学院・生命環境科学研究科, 教授 (80211788)

Co-Investigator(Kenkyū-buntansha) TSUMURA Yoshihiko  独立行政法人森林総合研究所, 森林遺伝研究領域, 室長 (20353774)
WASHITANI Izumi  東京大学, 大学院・農学生命科学研究科, 教授 (40191738)
Co-Investigator(Renkei-kenkyūsha) UENO Masayoshi  独立行政法人森林総合研究所, 研究員 (40414479)
KITAMOTO Naoko  筑波大学, 大学院・生命環境科学研究科, 研究員 (70447241)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥50,050,000 (Direct Cost: ¥38,500,000、Indirect Cost: ¥11,550,000)
Fiscal Year 2010: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Fiscal Year 2009: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2008: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Keywords生物多様性保全 / サクラソウ / 適応的遺伝子 / QTL解析 / ESTマーカー / 異型花柱性 / 遺伝子連鎖地図 / アソシエーション解析
Research Abstract

To develop the conservation strategy of endangered wild primroses, we tried to identify the adaptive genes by QTL analysis and the genome-wide gene analysis based on the expression genetic information. Several associated gene regions about hetero style which affected the continuation of the group were detected with high precision. We clarified that a bottle neck effect committed the process of domestication by a comparison between genetic diversity of wild primrose and cultivars. Using EST markers, genetic analysis about the adaptive characters including the budding date and the flowering time were performed for 7 populations from Hokkaido to Kanto region. We could detect a lot of loci about budding in conjunction with the adaptation of the primrose. The results of our study will contribute not only the development of the conservation strategy, but also will become the model which show how to use genome information for conservation strategy.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (54 results)

All 2011 2010 2009 2008 Other

All Journal Article (35 results) (of which Peer Reviewed: 30 results) Presentation (11 results) Book (4 results) Remarks (4 results)

  • [Journal Article] QTL analysis of heterostyly in Primula sieboldii and its application for morph identification in wild populations2011

    • Author(s)
      Yoshida, Y., S. Ueno, M. Honjo, N. Kitamoto, M. Nagai, I. Washitani, Y. Tsumura and R. Ohsawa
    • Journal Title

      Annals of Botany

      Volume: 108 Pages: 133-142

    • NAID

      120007137307

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Generation of expressed sequence tags,-development of microsatellite and single nucleotide polymorphism markers in Primula sieboldii E. Morren (Primulaceae) and its effectiveness for analysis of genetic diversity in natural and horticultural populations2011

    • Author(s)
      Ueno, S, Y. Yoshida, Y. Taguchi, T. Ujino-Ihara, N. Kitamoto, M. Honjo, R. Ohsawa, I. Washitani and Y. Tsumura
    • Journal Title

      Breeding Science

      Volume: 61 Pages: 234-243

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Post-pollination process in a partially self-compatible distylous plant, Primula sieboldii (Primulaceae)2011

    • Author(s)
      Nishihiro, J. and Washitani, I
    • Journal Title

      Plant species Biology

      Volume: 26 Pages: 213-220

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] QTL analysis of heterostyly in Primula sieboldii and its application for morph identification in wild populations2011

    • Author(s)
      Yoshida, Y, S.Ueno, Honjo M., N.Kitamoto, M.Nagai, I.Washitani, Y.Tsumura, Y.Yasui, R.Ohsawa
    • Journal Title

      Annals of Botany

      Volume: 印刷中

    • NAID

      120007137307

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Post-pollination process in a partially self-compatible distylous plant, Primula sieboldii(Primulaceae).2011

    • Author(s)
      Nishihiro, J., Washitani, I.
    • Journal Title

      Plant Species Biology.

      Volume: 印刷中

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Predicting the rate of range expansion of an invasive alienbumblebee(Bombus terrestris)using a stochastic spatiotemporal model2011

    • Author(s)
      Kadoya, T., Washitani, I.
    • Journal Title

      Biological Conservation

      Volume: 143 Pages: 1228-1235

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Predicting the rate of range expansion of an invasive alien bumblebee (Bombus terrestris) using a stochastic spatio-temporal model2010

    • Author(s)
      Kadoya, T. and Washitani, I
    • Journal Title

      Biological conservation

      Volume: 143 Pages: 1228-1235

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 人間中心世の変動環境と生物多様性2010

    • Author(s)
      鷲谷いづみ
    • Journal Title

      科学

      Volume: 10月号

    • Related Report
      2010 Final Research Report
  • [Journal Article] 気候変動対策と生物多様性保全2010

    • Author(s)
      鷲谷いづみ
    • Journal Title

      学術の動向

      Volume: 3月号 Pages: 76-81

    • NAID

      130000450807

    • Related Report
      2010 Final Research Report
  • [Journal Article] 生物多様性の危機とその克服に向けて2010

    • Author(s)
      鷲谷いづみ
    • Journal Title

      前衛

      Volume: 862 Pages: 209-231

    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Journal Article] 人間中心世の変動環境と生物多様性2010

    • Author(s)
      鷲谷いつみ
    • Journal Title

      科学

      Volume: 10月号 Pages: 1-1

    • Related Report
      2010 Annual Research Report
  • [Journal Article] 気候変動対策と生物多様腔保全2010

    • Author(s)
      鷲谷いつみ
    • Journal Title

      学術の動向

      Volume: 3月号 Pages: 76-81

    • Related Report
      2010 Annual Research Report
  • [Journal Article] 群馬県鳴神山における絶滅危惧植物カッコソウの個体群再生に向けた取り組みとそれにより明らかになった種特性及び生育環境2009

    • Author(s)
      大谷雅人, 西廣淳, 寺内浩, 寺内優美子, 鷲谷いづみ
    • Journal Title

      保全生態学研究

      Volume: 14 Pages: 91-99

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reconsideration for conservation units of wild Primula sieboldii in Japan based on adaptive diversity and molecular genetic diversity2009

    • Author(s)
      Yoshida, Y., M. Honjo, N. Kitamoto, R. Ohsawa
    • Journal Title

      Genetics Research

      Volume: 91 Pages: 225-235

    • NAID

      120007131477

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Toward the restoration osustainable population of a threatened aquatic plant, Nymphoides peltata : integrated genetic/demographic studies and practices2009

    • Author(s)
      Nishihiro, J., Uesugi, R., Takagawa, S., and Washitani, I
    • Journal Title

      Biological Conservation

      Volume: 142 Pages: 1906-1912

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Conservation ecological study study of invasion of Bombus terrestris(Hymenoptera : Apidae) into a preserved area of the Notsuke Peninsula of eastern Hokkaido, Japan2009

    • Author(s)
      Inoue, M.N., Ishikawa, S., Inoue, T. and Washitani, I
    • Journal Title

      Applied Entomology and Zoology

      Volume: 44 Pages: 337-342

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of 120 microsatellite markers for Primula sieboldii E. Morren for linkage mapping2009

    • Author(s)
      Ueno, S., Y. Yoshida, Y. Taguchi, M. Honjo, N. Kitamoto, I. Washitani, R. Ohsawa, Y. Tsumura
    • Journal Title

      Conservation genetics

      Volume: 10 Pages: 1945-1952

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reconsideration for conservation units of wild Primula sieboldii in Japan based on adaptive diversity and molecular genetic diversity2009

    • Author(s)
      Yoshida, Y., M.Honjo, N.Kitamoto, R.Ohsawa
    • Journal Title

      Genetics Research 91

      Pages: 225-235

    • NAID

      120007131477

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Toward the restoration of a sustainable population of a threatened aquatic plant, Nymphoides peltata : integrated genetic/demographic studies and practices.2009

    • Author(s)
      Nishihiro, J., Uesugi, R., Takagawa, S., Washitani, I.
    • Journal Title

      Biological Conservation 142

      Pages: 1906-1912

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Conservation ecological study of invasion of Bombus terrestris(Hymenoptera : Apidae)into a preserved area of the Notsuke Peninsula of eastern Hokkaido, Japan.2009

    • Author(s)
      Inoue, M.N., Ishikawa, S., Inoue, T., Washitani, I.
    • Journal Title

      Applied Entomology and Zoology 44

      Pages: 337-342

    • NAID

      130000143013

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tracing the origins of stocks of the endangered species Primula sieboldii using nuclear microsatellites and chloroplast DNA.2009

    • Author(s)
      Honjo, M, Ueno,S., Tsumura, Y., Handa, T., Washitani, I., and Ohsawa, R.
    • Journal Title

      Conservation Genetics 9

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ecological responses of Primula. nutans to centimeter-scale tonoffranhic and environmental variability in an aloine wetland.2009

    • Author(s)
      Shen, H., Tang, Y. and Washitani, I
    • Journal Title

      Ecological Research 24

      Pages: 75-81

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Management units of the endangered herb Primula sieboldii based on microsatellite variation among and within populations throughout Japan2008

    • Author(s)
      Honjo, M., N. Kitamoto, S. Ueno, YTsumura, I. Washitani, R. Ohsawa
    • Journal Title

      Conservation Genetics

      Volume: 10 Pages: 257-267

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Origins of traditional cultivars of Primula sieboldii revealed by nuclear microsatellite and chloroplast DNA variations2008

    • Author(s)
      Honjo, M., T. Handa, Y. Tsumura, I. Washitani, R. Ohsawa
    • Journal Title

      Breeding Science

      Volume: 58 Pages: 347-354

    • NAID

      110007007902

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tracing the origins of stocks of the endangered species Primula sieboldii using nuclear microsatellites and chloroplast DNA2008

    • Author(s)
      Honjo, M., S. Ueno, Y. Tsumura, T. Handa, I. Washitani, R. Ohsawa
    • Journal Title

      Conservation Genetics

      Volume: 9 Pages: 1139-1177

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genetic variation and differentiation of floral morphology in wild Primula Sieboldii evaluated by image analysis datand SSR markers2008

    • Author(s)
      Yoshida, Y., M. Honjo, N. Kitamoto, R. Ohsawa
    • Journal Title

      Breeding Science

      Volume: 58 Pages: 301-307

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Towards a legal framework for systematic conservation : identification and development of allele-specific PCR markers for conspecific varieties of an endangered perennial herb Primula kisoana Miquel based on sequence variation of chloroplast DNA2008

    • Author(s)
      Ohtani, M., Terauchi, H., Nishihiro, J., Ueno, S., Tsumura, Y., and Washitani, I
    • Journal Title

      conservation genetics

      Volume: 9 Pages: 1173-1181

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characteristics of leaf photosynthesis and simulated individual carbon budget in Primula nutans under contrasting light and temperature conditions2008

    • Author(s)
      Shen, H., Tang, Y., Muraoka, H. and Washitani, I
    • Journal Title

      Journal of Plant Research

      Volume: 121 Pages: 191-200

    • NAID

      10021087824

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genetic variation and differentiation of floral morphology in wild Primula sieboldii evaluated by image analysis data and SSR markers2008

    • Author(s)
      Yoshida, Y., M. Honjo, N. Kitamoto and R. Ohsawa
    • Journal Title

      Breeding Science 58

      Pages: 301-307

    • NAID

      110006879008

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of population density of compatible neighbors on inbreeding level within a Primula sieboldii population2008

    • Author(s)
      Kitamoto, N., Ueno, S., Tsumura, Y., Washitani, I, Ohsawa, R.
    • Journal Title

      Ecoloeical Research 23

      Pages: 307-315

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Towards a legal framework for systematic conservation: identification and development of allele-specific PCR markers for conspecific varieties of an endangered perennial herb Primula kisoana Miauel based on sequence variation of chloroplast DNA.2008

    • Author(s)
      Ohtani, M., Terauchi, H., Nishihiro, J., Ueno, S., Tsumura, Y., and Washitani, I.
    • Journal Title

      Conservation Genetics 9

      Pages: 1173-1181

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characteristics of leaf photosynthesis and simulated individual carbon budget in Primula nutans under contrasting light and temnerature conditions.2008

    • Author(s)
      Shen, H., Tang, Y., Muraoka, H. and Washitani, I.
    • Journal Title

      Journal of Plant Research 121

      Pages: 191-200

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of 120 microsatellite markers for Primula sieboldii E. Morren for linkage mapping

    • Author(s)
      Ueno, S., Yoshida, Y., Taguchi, Y., Honjo, M., Kitamoto, N., Washitani, I., Ohsawa, R. and Tsumura, Y.
    • Journal Title

      Conservation Genetics (In press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 群馬県鳴神山における絶滅危惧植物カッコソウの個体群再生に向けた取り組みとそれにより明らかになった種特性及び生育環境

    • Author(s)
      大谷雅人,西廣淳,寺内浩,寺内優美子,鷲谷いつみ
    • Journal Title

      保全生態学研究 (印刷中)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Management units of the endangered herb Primula sieboldii based on microsatellite variation among and within populations throughout Japan

    • Author(s)
      Honjo, M., Kitamoto, N., Ueno, S., Tsumura, Y., Was.hitani, I. and Ohsawa, R
    • Journal Title

      Conservation Genetics (In press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] QTL analysis of petal form variation in Primula sieboldii2010

    • Author(s)
      Ohsawa, R., Yoshida, Y., S. Ueno, Y. Tsumura
    • Organizer
      28th International Horticultural Congress
    • Place of Presentation
      リスボン、ポルトガル
    • Year and Date
      2010-08-26
    • Related Report
      2010 Final Research Report
  • [Presentation] QTL analysis of petal form variation in Primula sieboldi2010

    • Author(s)
      Ohsawa, R., Yoshida, Y., S.Ueno, Y.Tsumura
    • Organizer
      28^<th> International Horticultural Congress
    • Place of Presentation
      リスボン、ポルトガル
    • Year and Date
      2010-08-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] QTL analysis of floral morphology related heterostyly in Primula sieboldii2010

    • Author(s)
      Yoshida, Y., S. Ueno, Y. Tsumura and R. Ohsawa
    • Organizer
      28th International Horticultural Congress
    • Place of Presentation
      リスボン、ポルトガル
    • Year and Date
      2010-08-24
    • Related Report
      2010 Final Research Report
  • [Presentation] QTL analysis of floral morphology related heterostyly in Primula sieboldii2010

    • Author(s)
      Yoshida, Y., S.Ueno, Y.Tsumura, R.Ohsawa
    • Organizer
      28^<th> International Horticultural Congress
    • Place of Presentation
      リスボン、ポルトガル
    • Year and Date
      2010-08-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] サクラソウの異型花柱性に関するQTL解析2009

    • Author(s)
      吉田康子, 上野真義, 本城正憲, 北本尚子, 永井美穂子, 鷲谷いづみ, 津村義彦, 大澤良
    • Organizer
      日本育種学会
    • Place of Presentation
      北海道大学
    • Year and Date
      2009-09-26
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] SSRマーカー解析に基づく常緑性ツツジ九州野生集団の遺伝的構造と園芸品種群「江戸キリシマ」および「クルメツツジ」との関係2009

    • Author(s)
      宮脇実桜, 倉重祐二, 小林伸雄, Jan De Riek, 半田高, 大澤良
    • Organizer
      日本育種学会
    • Place of Presentation
      北海道大学
    • Year and Date
      2009-09-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] 適応に関連したQTLに基づく野生サクラソウ集団の遺伝的多様性評価2009

    • Author(s)
      吉田康子, 上野真義, 北本尚子, 小玉昌孝, 本城正憲, 田口由利子, 永井美穂子, 鷲谷いづみ, 津村義彦, 大澤良
    • Organizer
      日本生態学会第56回大会
    • Place of Presentation
      岩手県立大学
    • Year and Date
      2009-03-19
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] サクラソウの受粉から結実までに働く近交弱勢の関与遺伝子座数とその効果の解析2009

    • Author(s)
      北本尚子, 本城正憲, 吉田康子, 上野真義, 津村義彦, 鷲谷いづみ, 大澤良
    • Organizer
      日本生態学会第56回大会
    • Place of Presentation
      岩手県立大学
    • Year and Date
      2009-03-19
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] サクラソウの個体群構造と種子生産の空間的不均一性2009

    • Author(s)
      田中良典, 鷲谷いづみ
    • Organizer
      日本生態学会第56回大会
    • Place of Presentation
      岩手県立大学
    • Year and Date
      2009-03-19
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] Mii Unreduced gamete formation and its utilization for ornamental plant breeding2008

    • Author(s)
      Kato, J., M. Nimura, Y. Futagami, M. Ikeda, M. Hayashi, Y. Murata, Y. Yoshida, R. Ohsawa, S.Ichihashi, M
    • Organizer
      The 3rd Asian Chromosome Colloquium. Abstract
    • Year and Date
      2008-12-03
    • Related Report
      2010 Final Research Report
  • [Presentation] SSRマーカーによる3倍体サクラソウ(Primula Sieboldii)種間雑種形成に寄与する非還元雌性配偶子タイプの推定2008

    • Author(s)
      加藤淳太郎, 村田有紀, 鈴木幹奈, 池田真由子, 吉田康子, 上野真義, 大澤良
    • Organizer
      育種学研究10(別2)
    • Year and Date
      2008-10-11
    • Related Report
      2010 Final Research Report
  • [Book] 保全性多額の技法2010

    • Author(s)
      鷲谷いづみ, 宮下直, 西廣淳, 角谷拓
    • Publisher
      東京大学出版会
    • Related Report
      2010 Final Research Report
  • [Book] 保全生態学の技法・調査・研究・実践マニュアル2010

    • Author(s)
      鷲谷いづみ・宮下直・西廣淳・角谷拓
    • Total Pages
      336
    • Publisher
      東京大学出版会
    • Related Report
      2009 Annual Research Report
  • [Book] サクラソウのひみつ2008

    • Author(s)
      幸田シャーミン(作)・大島節子(絵)・鷲谷いづみ(監修)
    • Publisher
      社団法人ユネスコ協会連盟
    • Related Report
      2010 Final Research Report
  • [Book] サクラソウのひみつ2008

    • Author(s)
      幸田シャーミン(作),大島節子(絵),鷲谷いつみ(監修)
    • Publisher
      社団法人ユネスコ協会連盟(印刷中)
    • Related Report
      2008 Annual Research Report
  • [Remarks] 大澤良

    • URL

      http://www.sakura.cc.tsukuba.ac.jp/~pbreed/

    • Related Report
      2010 Final Research Report
  • [Remarks] 津村義彦

    • URL

      http://www.ffpri.affrc.go.jp/research/dept/09f-genetics/index.html

    • Related Report
      2010 Final Research Report
  • [Remarks] 鷲谷いづみ

    • URL

      http://www.coneco.es.a.u-tokyo.ac.jp/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • Related Report
      2008 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi