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Community analysis of subsurface biosphere responsible for methane cycling

Research Project

Project/Area Number 20244089
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Geochemistry/Astrochemistry
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

KAMAGATA Yoichi  National Institute of Advanced Industrial Science and Technology, 生物プロセス研究部門, 研究部門長 (70356814)

Co-Investigator(Kenkyū-buntansha) TAMAKI Hideyuki  独立行政法人産業技術総合研究所, 生物プロセス研究部門, 研究員 (00421842)
SAKATA Susumu  独立行政法人産業技術総合研究所, 地圏資源環境研究部門, 研究グループ長 (70357101)
YOSHIOKA Hideyoshi  独立行政法人産業技術総合研究所, 地圏資源環境研究部門, 研究員 (30415765)
TAKEUCHI Mio  独立行政法人産業技術総合研究所, 地圏資源環境研究部門, 研究員 (20357403)
SUZUKI Yohei  独立行政法人産業技術総合研究所, 地圏資源環境研究部門, 研究員 (00359168)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥48,490,000 (Direct Cost: ¥37,300,000、Indirect Cost: ¥11,190,000)
Fiscal Year 2010: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2009: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000)
Fiscal Year 2008: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Keywords天然ガス / 油田 / 地下圏生物 / メタン菌 / メタン酸化菌 / 地球化学 / メタン生成古細菌 / 地下圏微生物 / 水溶性天然ガス / ゲノム解析 / 水素・酢酸からのメタン生成 / 嫌気的メタン酸化 / 水素からのメタン生成
Research Abstract

To shed light on the subsurface methane cycling microbial communities, we have analyzed the community structures, cultivated the dominant organisms, and analyzed the basic traits of isolated organisms : (1) we were successful in isolating dominant hydrogenotrophic methanogens in an association water sample in a natural gas field. The isolate was found to be distinct from know methanogens ; (2) acetate was found to be the most important intermediate in a deep subsurface oil reservoir in which syntrophic acetate oxidation occurred ; (3) anaerobic methane oxidation was detected in terrestrial subsurface samples ; (4) a syntrophic aerobic methane oxidizing community that comprised at least three members of bacteria was constructed.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (32 results)

All 2011 2010 2009 Other

All Journal Article (11 results) (of which Peer Reviewed: 10 results) Presentation (16 results) Book (2 results) Remarks (3 results)

  • [Journal Article] A distinct freshwater-adapted subgroup of ANME-1 dominates active archaeal communities in terrestrial subsurfaces in Japan.2011

    • Author(s)
      Takeuchi, M., H.Yoshioka, Y.Seo, S.Tanabe, H.Tamaki, H.A.Takahashi, S.Igari, D.Mayumi, S.Sakata
    • Journal Title

      Environmental Microbiology in press

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A six-well plate method: less laborious and effective method for cultivation of obligate anaerobic microorganisms.2011

    • Author(s)
      Nakamura, K., H.Tamaki, M.S.Kang, H.Mochimaru, S-T Lee, K.Nakamura, Y.Kamagata
    • Journal Title

      Microbes and Environments published on line

    • NAID

      10029978006

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Evidence for syntrophic acetate oxidation coupled to hydrogenotrophic methanogenesis in the high-temperature petroleum reservoir of Yabase oil field (Japan).2011

    • Author(s)
      Mayumi, D., H.Mochimaru, S.Sakata, H.Maeda, Y.Miyagawa, M.Ikarashi, M.Takeuchi, Y.Kamagata
    • Journal Title

      Environmental Microbiology published on line

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A distinct subgroup of ANME-1 dominates active archaeal communities in terrestrial freshwater subsurfaces in Japan.2011

    • Author(s)
      竹内美緒、吉岡秀佳、鎌形洋一, 坂田将, 他
    • Journal Title

      Environmental Microbiology

      Volume: (掲載確定)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Research for microbial conversion of residual oil into methane in depleted oil fields to develop new EOR process.2010

    • Author(s)
      Maeda H., Y.Miyagawa, M.Ikarashi, D.Mayumi, H.Mochimaru, H.Yoshioka, S.Sakata H.Kobayashi, K.Sato
    • Journal Title

      Proceedings of Abudabi Oil and Gas Conference and Exhibition 2010, Abudabi. OnePetro, No.138135-MS

    • Related Report
      2010 Final Research Report
  • [Journal Article] Evidence for syntrophic acetate oxidation coupled to hydrogenotrophic methanogenesis in the high-temperature petroleum reservoir of Yabase oil field(Japan).2010

    • Author(s)
      眞弓大介, 坂田将、竹内美緒、吉岡秀佳、鎌形洋一, 他
    • Journal Title

      Environmental Microbiology

      Volume: (pubhshed online)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cultivation of methanogens under low hydrogen conditions by using the coculture method.2009

    • Author(s)
      Sakai, S., H.Imachi, Y.Sekiguchi, I-Cheng Tseng, A.Ohashi, H.Harada, Y.Kamagata
    • Journal Title

      Applied and Environmental Microbiology 75

      Pages: 4892-4896

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Methanolobus profundi sp. nov., a new methylotrophic methanogen isolated from deep subsurface sediments in a natural gas field.2009

    • Author(s)
      Mochimaru, H, H.Tamaki, S.Hanada, H.Imachi, K.Nakamura, S.Sakata, Y.Kamagata
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiolology 58

      Pages: 714-718

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Metthanolobus profundi sp. nov., a methylotrophic methanogen isolated from deep subsurface sediments in a natural gas field2009

    • Author(s)
      持丸華子、鎌形洋一, ほか
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology

      Volume: 58 Pages: 714-718

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Apparent richness and community composition of Bacteria and Archaea in geothermal springs.2009

    • Author(s)
      Kormas, K., 玉木秀幸, 鎌形洋一, ほか
    • Journal Title

      Aquatic Microbial Ecology

      Volume: 57 Pages: 113-122

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cultivation of methanogens under low hydrogen conditions by using the coculture method2009

    • Author(s)
      酒井早苗、鎌形洋一, ほか
    • Journal Title

      Applied and Environmental Microbiology 75

      Pages: 4892-4896

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Cultivation and large-scale community analysis of methantrophic organisms.2011

    • Author(s)
      鎌形洋一
    • Organizer
      Hong Kong University special lecture.
    • Place of Presentation
      Hong Kong University
    • Year and Date
      2011-03-19
    • Related Report
      2010 Final Research Report
  • [Presentation] Cultivation and large-scale community analysis of methantrophic organisms2011

    • Author(s)
      鎌形洋一
    • Organizer
      Hong Kong University Specia Hecture
    • Place of Presentation
      香港大学
    • Year and Date
      2011-03-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] メタゲノムアプローチで見える環境負荷変動による微生物群集の遷移2011

    • Author(s)
      鎌形洋一
    • Organizer
      日本生態学会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2011-03-12
    • Related Report
      2010 Annual Research Report
  • [Presentation] Anaerobic oxidation of methane in the terrestrial subsurface environment.2010

    • Author(s)
      Takeuchi M., H.Yoshioka, Y.Seo, S.Tanabe, H.Tamaki , Y.Kamagata, H.A.Takahashi, S.Igari, D.Mayumi, S.Sakata
    • Organizer
      AGU fall meeting,
    • Place of Presentation
      San Francisco
    • Year and Date
      2010-12-14
    • Related Report
      2010 Final Research Report
  • [Presentation] 安定同位体トレーサー法を用いた油層内共生微生物によるメタン生成経路の解明2010

    • Author(s)
      眞弓大介・坂田将・前田治男・宮川喜洋・五十嵐雅之
    • Organizer
      第2回メタンハイドレート総合シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2010-12-02
    • Related Report
      2010 Final Research Report
  • [Presentation] 深部地下油層環境における原油分解とメタン生成2010

    • Author(s)
      持丸華子・眞弓大介・吉岡秀佳・坂田将,玉木秀幸・鎌形洋一
    • Organizer
      第26回日本微生物生態学会
    • Place of Presentation
      つくば,茨城
    • Year and Date
      2010-11-24
    • Related Report
      2010 Final Research Report
  • [Presentation] :安定同位体トレーサー法を用いた油層内共生微生物によるメタン生成経路の解明2010

    • Author(s)
      眞弓大介・坂田将・前田治男・宮川喜洋・五十嵐雅之
    • Organizer
      第26回日本微生物生態学会
    • Place of Presentation
      つくば,茨城
    • Year and Date
      2010-11-24
    • Related Report
      2010 Final Research Report
  • [Presentation] Raman-FISHを用いた微生物共生系における炭素・窒素の移動経路の解明2010

    • Author(s)
      徐維那・竹内美緒・鎌形洋一・坂田将
    • Organizer
      第26回日本微生物生態学会大会
    • Place of Presentation
      つくば,茨城
    • Year and Date
      2010-11-24
    • Related Report
      2010 Final Research Report
  • [Presentation] Syntrophy based upon interspecies H2 transfer in anaerobic digestion.2010

    • Author(s)
      鎌形洋一
    • Organizer
      International Symposium on Environment and Renewable Energy.
    • Place of Presentation
      Pusan National Univ.
    • Year and Date
      2010-10-25
    • Related Report
      2010 Final Research Report
  • [Presentation] Syntrophy based upon interspecies H2 trmsfer in anaerobic digestion2010

    • Author(s)
      鎌形洋一
    • Organizer
      International Symposium on Environment and Renewable Energy
    • Place of Presentation
      韓国釜山大学
    • Year and Date
      2010-10-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] Anaerobic oxidation of methane in the terrestrial subsurface environment.2010

    • Author(s)
      Takeuchi M., H.Yoshioka, Y.Seo, S.Tanabe, H.Tamaki, Y.Kamagata, H.A.Takahashi, S.Igari, D.Mayumi, S.Sakata
    • Organizer
      13^<th> International Symposium on Microbial Ecology.
    • Place of Presentation
      Seatle, USA
    • Year and Date
      2010-08-24
    • Related Report
      2010 Final Research Report
  • [Presentation] Methanogenic pathway in a high-temperature petroleum reservoir assessed by stable isotope tracer and molecular biological techniques.2010

    • Author(s)
      眞弓大介, 坂田将, 坂田将, 宮川喜洋, 五十嵐雅之
    • Organizer
      13^<th> International Symposium on Microbial Ecology.
    • Place of Presentation
      Seatle, USA
    • Year and Date
      2010-08-24
    • Related Report
      2010 Final Research Report
  • [Presentation] 陸域地下圏における微生物による嫌気的メタン酸化の解明2010

    • Author(s)
      竹内美緒・吉岡秀佳・徐維那・田邉晋・玉木秀幸・鎌形洋一・高橋浩・猪狩俊一郎・眞弓大介・坂田将
    • Organizer
      2010年度日本地球惑星科学連合大会
    • Place of Presentation
      幕張,千葉
    • Year and Date
      2010-05-25
    • Related Report
      2010 Final Research Report
  • [Presentation] メタン生成古細菌を中心とした微生物間共生機構の解明2009

    • Author(s)
      鎌形洋一
    • Organizer
      東大生物生産工学研究センターシンポジウム
    • Place of Presentation
      東京大学
    • Year and Date
      2009-12-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] Ecology of methanogenic archaea2009

    • Author(s)
      鎌形洋一
    • Organizer
      中国微生物生態学会
    • Place of Presentation
      浙江省寧波市
    • Year and Date
      2009-11-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] 無酸素環境下に広がる微生物間共生の世界2009

    • Author(s)
      鎌形洋一
    • Organizer
      日本農芸化学会支部会
    • Place of Presentation
      北海道大学
    • Year and Date
      2009-07-24
    • Related Report
      2009 Annual Research Report
  • [Book] 難培養微生物研究の最新技術II(シーエムシー出版)2011

    • Author(s)
      鎌形洋一
    • Total Pages
      300
    • Publisher
      難培養微生物の培養技術
    • Related Report
      2010 Annual Research Report
  • [Book] 難培養微生物研究の最新技術II2010

    • Author(s)
      鎌形洋一
    • Publisher
      シーエムシー出版(分担執筆)
    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://unit.aist.go.jp/bpri/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://unit.aist.go.jp/georesenv/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://unit.aist.go.jp/bpri/

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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