| Budget Amount *help |
¥47,710,000 (Direct Cost: ¥36,700,000、Indirect Cost: ¥11,010,000)
Fiscal Year 2011: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2009: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
Fiscal Year 2008: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
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| Research Abstract |
To quantitatively detect point gene mutation, we have developed an affinity capillary electrophoresis using a block copolymer probe composed of a synthetic polymer and a single-stranded DNA. During electrophoresis of a mixture of the target and nontarget DNA, the affinity probe binds selectively to the target DNA and thus decreases the electrophoretic mobility by exerting a large amount of hydrodynamic friction. Moreover, this study demonstrated that(1) the peak resolution was improved in the presence of electroosmotic flow and(2) a block copolymer probe containing peptide nucleic acid sequence-selectively bound to secondary-structured target DNA.
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