| Budget Amount *help |
¥49,530,000 (Direct Cost: ¥38,100,000、Indirect Cost: ¥11,430,000)
Fiscal Year 2011: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2010: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2009: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2008: ¥21,060,000 (Direct Cost: ¥16,200,000、Indirect Cost: ¥4,860,000)
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| Research Abstract |
We have found the isolation of bacterial strains capable of degrading fluorinated materials, 8 strains of Actinobacteria exhibited degradability of ethyl difluoroacetate(DFAc) was accumulated by bacteria, giving difluoroacetic acid and then fluoride ion. Further, 13 strains of Actinobacteria exhibited degradability of fluorobenzene and/or benzotrifluoride. In batch culture, growth of strains on fluorinated materials led to the release of fluoride ion. We have established the one-pot three-step continuous enzymatic synthesis of 5-fluoro-5-deoxy-D-ribose(5-FDR) from ATP and L-methionine using S-adenosyl-L-methionine synthase(MetK), fluorinase and methylthioadenosinenucleosidase(MtnN) in the presence of fluoride ion. Especially, for the purpose of thesustainable development of fluorine chemistry, we have established the reuse of fluoride ion generated from BF4 ionic liquids and/or the biodegradation of BTF in the one-pot synthetic process to 5-FDR. Further, we have established the retransformation of fluoride ion generated from biodegradation of BTF to calcium fluoride.
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