Evaluation and dynamic measurement of biomembrane transporter using on-chip tethered microtool

• Project/Area Number: 20246044
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Intelligent mechanics/Mechanical systems
• Research Institution: Tohoku University
• Principal Investigator: ARAI Fumihito Tohoku University, 工学研究科, 教授 (90221051)
• Co-Investigator(Kenkyū-buntansha): UOZUMI Nobuyuki 東北大学, 大学院・工学研究科, 教授 (40223515)
• Co-Investigator(Renkei-kenkyūsha): MARUYAMA Hisataka 名古屋大学, 大学院・工学研究科, 助教 (60377843)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥49,790,000 (Direct Cost: ¥38,300,000、Indirect Cost: ¥11,490,000); Fiscal Year 2010: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000); Fiscal Year 2009: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000); Fiscal Year 2008: ¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000)
• Keywords: マイクロ・ナノデバイス / 光ピンセット / 先端機能デバイス / ナノバイオ / フォトリソグラフィ / イオンチャネル / マイクロ流体デバイス / 遺伝子操作

Research Abstract

We promoted the research on basic technologies for on-chip single cell analysis to measure the cell variations induced by control of the environmental conditions and physical stimulus using functional microtool. We fabricated the microfluidic chip having local cell cage by micro-processing based on photofabrication. Functional microtools such as tethered shape tool, flexible tool and sensing tool, were also fabricated. We succeeded in quantitative evaluation of biomembrane transporter by measuring the volume variation of the cell induced by osmotic stimulus on this system.

Research Products

• [Journal Article] The KtrA and KtrE subunits are required for Na+ dependent K+ uptake by KtrB across the plasma membrane in Synechocystis sp.PCC6803. (2010)
  • Author(s): Zulkifli, L., Akai, M., Yoshikawa, A., Shimojima, M., Ohta, H., Guy HR., Uozumi, N.
  • Journal Title: The Journal of Bacteriology Vol.192 Pages: 5063-5070
  • Peer Reviewed
• [Journal Article] Optical Adhesion Control of Hydrogel Microtools for On Demand Immobilization and Measurement of Cells on a Microfluidic Chip (2010)
  • Author(s): H.Maruyama, T.Fukuda, F.Arai
  • Journal Title: Journal of Robotics and Mechatronics Vol.22 Pages: 631-638
  • Peer Reviewed
• [Journal Article] Massive Parallel Assembly of Microbeads for Fabrication of Microtools Having Spherical Structure and Powerful Manipulation by Optical Tweezers (2010)
  • Author(s): H.Maruyama, R.Iitsuka, K. Onda, F.Arai
  • Journal Title: Journal of Robotics and Mechatronics Vol.22 Pages: 356-362
  • Peer Reviewed
• [Journal Article] Size-Dependent Filtration and Trapping of Microparticles in a Microfluidic Chip Using Graduated Gaps and Centrifugal Force (2010)
  • Author(s): H.Maruyama, S.Sakuma, Y.Yamanishi, F.Arai
  • Journal Title: Journal of Robotics and Mechatronics Vol.22 Pages: 280-285
  • Peer Reviewed
• [Journal Article] Optical Adhesion Control of Hydrogel Microtools for On-Demand Immobilization and Measurement of Cells on a Microfluidic Chip (2010)
  • Author(s): H.Maruyama, T.Fukuda, F.Arai
  • Journal Title: Journal of Robotics and Mechatronics Volume: 22 Pages: 631-638
  • Peer Reviewed
• [Journal Article] Massive Parallel Assembly of Microbeads for Fabrication of Microtools Having Spherical Structure and Powerful Manipulation by Optical Twee zers (2010)
  • Author(s): H.Maruyama, R.Iitsuka, K.Onda, F.Arai
  • Journal Title: Journal of Robotics and Mechatronics Volume: 22 Pages: 356-362
  • Peer Reviewed
• [Journal Article] Size-Dependent Filtration and Trapping of Microparticles in a Microfluidic Chip Using Graduated Gaps and Centrifugal Force (2010)
  • Author(s): H.Maruyama, S.Sakuma, Y.Yamanishi, F.Arai
  • Journal Title: Journal of Robotics and Mechatronics Volume: 22 Pages: 280-285
  • Peer Reviewed
• [Journal Article] The KtrA and KtrE subunits are required for Na^+ dependent K^+ uptake by KtrB across the plasma membrane in Synechocystis sp.PCC 6803. (2010)
  • Author(s): Zulkifli, L., Akai, M., Yoshikawa, A., Shimojima, M., Ohta, H., Guy HR., Uozumi, N.
  • Journal Title: The Journal of Bacteriology Volume: 192 Pages: 5063-5070
  • Peer Reviewed
• [Journal Article] Size-Dependent Filtration and Trapping of Microparticles in a Microfluidic Chip Using Graduated Gaps and Centrifugal Force (2010)
  • Author(s): Hisataka Maruyama, Shinya Sakuma, Yoko Yamanishi, Fumihito Arai
  • Journal Title: Journal.of Robotics and Mechatronics 22(掲載確定)
  • Peer Reviewed
• [Journal Article] Massive Parallel Assembly of Microbeads for Fabrication of Microtools Having Spherical Structure and Powerful Manipulation by Optical Tweezers (2010)
  • Author(s): Hisataka Maruyama, Ryo Iitsuka, Kazuhisa Onda, Fumihito Arai
  • Journal Title: Journal of Robotics and Mechatronics 22(掲載確定)
  • Peer Reviewed
• [Journal Article] Threonine at position 306 of the KAT1 potassium channel is essential for channel activity and is a target site for ABA-activated SnRK2/OST1/SnRK2.6 protein kinase (2009)
  • Author(s): Sato, A., Sato, Y., Fukao, Y., Fujiwara, M.Umezawa, T., Shinozaki, K., Hibi, T., Taniguchi, M, Miyake, H., Goto, D.B., Uozumi, N.
  • Journal Title: Biochem.J. Vol.22 Pages: 439-448
  • Peer Reviewed
• [Journal Article] Threonine at position 306 of the KAT1 potassium channel is essential for channel activity and is a target site for ABA-activated SnRK2/OST1/SnRK2.6 protein kinase (2009)
  • Author(s): Sato, A., Sato, Y., Fukao, Y, Fujiwara, M., Umezawa, T., Shinozaki, K., Hibi, T., Taniguchi, M., Miyake, H., Goto, D.B., Uozumi, N.
  • Journal Title: Biochem.J. 73 Pages: 439-448
  • Peer Reviewed
• [Presentation] Massive Parallel Assembly of Microbeads for Fabrication of Microtools Having Spherical Structure and Powerful Laser Manipulation (2011)
  • Author(s): H.Maruyama, R.Iitsuka, K.Onda, F.Arai
  • Organizer: 2010 IEEE International Conference on Robotics and Automation
  • Place of Presentation: Anchorage, USA
  • Year and Date: 2011-05-04
• [Presentation] Evaluation of Thermal Conductivity of Single Carbon Nanotube Using Functional Gel Temperature Sensor in Liquid (2011)
  • Author(s): Kyohei Tomita, Hisataka Maruyama, Fumihito Arai
  • Organizer: 第40回記念フラーレン・ナノチューブ総合シンポジウム
  • Place of Presentation: 名古屋,名城大学
  • Year and Date: 2011-03-10
• [Presentation] Massive Parallel Assembly of Microbeads for Fabrication of Microtools Having Spherical Structure and Powerful Laser Manipulation (2010)
  • Author(s): 丸山央峰, 飯塚龍, 恩田一寿, 新井史人
  • Organizer: 2010 IEEE International Conference on Robotics and Automation
  • Place of Presentation: Anchorage, USA
  • Year and Date: 2010-05-04
• [Presentation] STZE-DEPENDENT FILTRATION AND TRAP OF MICROPARTICLES USING CENTRIFUGAL FORCE AND GRADUATED MECHANICAL GAP IN MICROFLUIDIC CHIP (2009)
  • Author(s): 丸山央峰, 佐久間臣耶, 山西陽子, 新井史人
  • Organizer: The 13th International Conference on Miniaturized Systems for Chemistry and Life Selenees
  • Place of Presentation: Korea, JEJU
  • Year and Date: 2009-11-04
• [Presentation] SIZE-DEPENDENT FILTRATION AND TRAP OF MICROPARTICLES USING CENTRIFUGAL FORCE AND GRADUATED MECHANICAL GAP IN MICROFLUIDIC CHIP (2009)
  • Author(s): Hisataka Maruyama, Shinya Sakuma, Yoko Yamanishi, Fumihito Arai
  • Organizer: The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
  • Place of Presentation: Korea, JEJU
  • Year and Date: 2009-11-04
• [Presentation] Size-Dependent Microparticle Filtration Using Magnetically Driven Microtool for Producing Gel-Microtool (2009)
  • Author(s): Benoit Chapurlat, Hisataka Maruyama, Yoko Yamanishi, Kyosuke Kotani, Fumihito Arai
  • Organizer: The 2009 IEEE/RSJ International Conference on Intelligent Robots and Systems
  • Place of Presentation: アメリカ,セントルイス
  • Year and Date: 2009-10-15
• [Presentation] レーザ駆動Robot-on-a-chipに関する研究-その3:テザード型マイクロツールの設計と製作- (2009)
  • Author(s): 猪股直生, 丸山央峰, 新井史人
  • Organizer: 日本機械学会ロボティクス・メカトロニクス講演会2009
  • Place of Presentation: 福岡,福岡国際会議場
  • Year and Date: 2009-05-26
• [Presentation] レーザ駆動Robot-on-a-chipに関する研究-その1:統合型光ピンセットのバイラテラル制御システム- (2009)
  • Author(s): 恩田一寿, 丸山央峰, 新井史人
  • Organizer: 日本機械学会ロボティクス・メカトロニクス講演会2009
  • Place of Presentation: 福岡,福岡国際会議場
  • Year and Date: 2009-05-26
• [Presentation] 磁気駆動Robot-on-a-chipに関する研究-その3:磁気駆動マイクロツールを用いた微粒子の連続選別- (2009)
  • Author(s): 丸山央峰, 佐久間臣耶, シャプラブノア, 山西陽子, 新井史人
  • Organizer: 日本機械学会ロボティクス・メカトロニクス講演会2009
  • Place of Presentation: 福岡,福岡国際会議場
  • Year and Date: 2009-05-26
• [Presentation] Multi-beam Laser Micromanipulation of Microtool by Integrated Optical Tweezers (2009)
  • Author(s): Fumihito Arai, Kazuhisa Onda, Ryolitsuka, Hisataka Maruyama
  • Organizer: 2009 IEEE International Conference on Robotics and Automation
  • Place of Presentation: Kobe, Kobe International Conference Center
  • Year and Date: 2009-05-15
• [Presentation] マイクロ流路を用いたラン藻の高浸透圧ストレスに対する適応機構のリアルタイム観察 (2009)
  • Author(s): 四十九俊彰
  • Organizer: 日本植物生理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-21
• [Presentation] 遠心力と磁気駆動マイクロツールを用いたオンチップ粒子選別 (2008)
  • Author(s): 丸山央峰
  • Organizer: 第18回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 京都大学桂キャンパス,船井哲良記念講堂
  • Year and Date: 2008-12-09
• [Presentation] 遠心力と磁気駆動マイクロツールを用いたオンチップ粒子選別 (2008)
  • Author(s): 丸山央峰
  • Organizer: 第18回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 京都大学桂キャンパス, 船井哲良記念講堂
  • Year and Date: 2008-12-09
• [Presentation] Production of Functional Microtool Using Size-Classified Gel-Microbeads (2008)
  • Author(s): Benoit Chapurlat
  • Organizer: 2008 International Symposium on Micro-NanoMechatronics and Human Science
  • Place of Presentation: 名古屋,名古屋大学野依記念学術交流館
  • Year and Date: 2008-11-08
• [Presentation] Production of Functional Microtool Using Size-Classified Gel-Microbeads (2008)
  • Author(s): Benoit Chapurlat
  • Organizer: 2008 International Symposium on Micro-NanoMechatronics and Human Science
  • Place of Presentation: 名古屋, 名古屋大学野依記念学術交流館
  • Year and Date: 2008-11-08
• [Presentation] MTCROPATTERNTNG OF HYDROGEL AND ON-CHIP LONG TIME MONITORING OF INDIVIDLAL CELLS IN A CAGE (2008)
  • Author(s): 新井史人
  • Organizer: The 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences
  • Place of Presentation: アメリカ,サンディエゴ
  • Year and Date: 2008-10-15
• [Presentation] MICROPATTERNING OF HYDROGEL AND ON-CHIP LONG TIME MONITORING OF INDIVIDUAL CELLS IN ACAGE (2008)
  • Author(s): Fumihito Arai
  • Organizer: The 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences
  • Place of Presentation: アメリカ, サンディエゴ
  • Year and Date: 2008-10-15
• [Presentation] フォトリソグラフィによるマイクロツールの作製とマルチビーム統合型光ピンセットによる操作 (2008)
  • Author(s): 飯塚龍
  • Organizer: 第26回日本ロボット学会学術講演会
  • Place of Presentation: 神戸市,神戸大学
  • Year and Date: 2008-09-09
• [Presentation] フォトリソグラフィによるマイクロツールの作製とマルチビーム統合型光ピンセットによる操作 (2008)
  • Author(s): 飯塚龍
  • Organizer: 第26回日本ロボット学会学術講演会
  • Place of Presentation: 神戸市, 神戸大学
  • Year and Date: 2008-09-09
• [Presentation] マイクロ流路を用いたラン藻の浸透圧適応のリアルタイム観察 (2008)
  • Author(s): 四十九俊彰
  • Organizer: 日本生物工学会
  • Place of Presentation: 仙台
  • Year and Date: 2008-08-28
• [Remarks]
  • URL: http://www.bioroboties.mech.nagoya-u.ac.jp/research/Robomec09/Robome09_Iitsuka.pdf
• [Remarks]
  • URL: http://www.bioroboties.mech.nagoya-u.ac.jp/research/Robomec09/Robomec09_Maruyama.pdf
• [Remarks]
  • URL: http://www.imech.mech.tohoku.ac.jp/research/Robomec09/Robomec09_Onda.pdf
• [Remarks]
  • URL: http://www.imech.mech.tohoku.ac.jp/research/Robomec09/Robomec09_Inomata.pdf
• [Remarks]
  • URL: http://www.bioroboties.mech.nagoya-u.ac.jp/research/40th_FNTS/40th_FNTS_Tomita.pdf
• [Remarks]
  • URL: http://www.biorobotics.mech.nagoya-u.ac.jp/research/Robomec09/Robomec09_Iitsuka.pdf
• [Remarks]
  • URL: http://www.biorobotics.mech.nagoya-u.ac.jp/research/Robomec09/Robomec09_Maruyama.pdf
• [Remarks]
  • URL: http://www.biorobotics.mech.nagoya-u.ac.jp/research/40th_FNTS/40th_FNTS_Tomita.pdf
• [Remarks]
  • URL: http://www.imech.mech.tohoku.ac.jp/research/Robomec09/Robomec09_Onda.pdf
• [Remarks]
  • URL: http://www.imech.mech.tohoku.ac.jp/research/Robomec09/Robomec09_Inomata.pdf
• [Remarks]
  • URL: http://www.imech.mech.tohoku.ac.jp/research/Robomec09/Robomec09_Maruyama.pdf