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Evaluation and dynamic measurement of biomembrane transporter using on-chip tethered microtool

Research Project

Project/Area Number 20246044
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Intelligent mechanics/Mechanical systems
Research InstitutionTohoku University

Principal Investigator

ARAI Fumihito  Tohoku University, 工学研究科, 教授 (90221051)

Co-Investigator(Kenkyū-buntansha) UOZUMI Nobuyuki  東北大学, 大学院・工学研究科, 教授 (40223515)
Co-Investigator(Renkei-kenkyūsha) MARUYAMA Hisataka  名古屋大学, 大学院・工学研究科, 助教 (60377843)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥49,790,000 (Direct Cost: ¥38,300,000、Indirect Cost: ¥11,490,000)
Fiscal Year 2010: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
Fiscal Year 2009: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
Fiscal Year 2008: ¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000)
Keywordsマイクロ・ナノデバイス / 光ピンセット / 先端機能デバイス / ナノバイオ / フォトリソグラフィ / イオンチャネル / マイクロ流体デバイス / 遺伝子操作
Research Abstract

We promoted the research on basic technologies for on-chip single cell analysis to measure the cell variations induced by control of the environmental conditions and physical stimulus using functional microtool. We fabricated the microfluidic chip having local cell cage by micro-processing based on photofabrication. Functional microtools such as tethered shape tool, flexible tool and sensing tool, were also fabricated. We succeeded in quantitative evaluation of biomembrane transporter by measuring the volume variation of the cell induced by osmotic stimulus on this system.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (43 results)

All 2011 2010 2009 2008 Other

All Journal Article (12 results) (of which Peer Reviewed: 12 results) Presentation (20 results) Remarks (11 results)

  • [Journal Article] The KtrA and KtrE subunits are required for Na+ dependent K+ uptake by KtrB across the plasma membrane in Synechocystis sp.PCC6803.2010

    • Author(s)
      Zulkifli, L., Akai, M., Yoshikawa, A., Shimojima, M., Ohta, H., Guy HR., Uozumi, N.
    • Journal Title

      The Journal of Bacteriology Vol.192

      Pages: 5063-5070

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Optical Adhesion Control of Hydrogel Microtools for On Demand Immobilization and Measurement of Cells on a Microfluidic Chip2010

    • Author(s)
      H.Maruyama, T.Fukuda, F.Arai
    • Journal Title

      Journal of Robotics and Mechatronics Vol.22

      Pages: 631-638

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Massive Parallel Assembly of Microbeads for Fabrication of Microtools Having Spherical Structure and Powerful Manipulation by Optical Tweezers2010

    • Author(s)
      H.Maruyama, R.Iitsuka, K. Onda, F.Arai
    • Journal Title

      Journal of Robotics and Mechatronics Vol.22

      Pages: 356-362

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Size-Dependent Filtration and Trapping of Microparticles in a Microfluidic Chip Using Graduated Gaps and Centrifugal Force2010

    • Author(s)
      H.Maruyama, S.Sakuma, Y.Yamanishi, F.Arai
    • Journal Title

      Journal of Robotics and Mechatronics Vol.22

      Pages: 280-285

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Optical Adhesion Control of Hydrogel Microtools for On-Demand Immobilization and Measurement of Cells on a Microfluidic Chip2010

    • Author(s)
      H.Maruyama, T.Fukuda, F.Arai
    • Journal Title

      Journal of Robotics and Mechatronics

      Volume: 22 Pages: 631-638

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Massive Parallel Assembly of Microbeads for Fabrication of Microtools Having Spherical Structure and Powerful Manipulation by Optical Twee zers2010

    • Author(s)
      H.Maruyama, R.Iitsuka, K.Onda, F.Arai
    • Journal Title

      Journal of Robotics and Mechatronics

      Volume: 22 Pages: 356-362

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Size-Dependent Filtration and Trapping of Microparticles in a Microfluidic Chip Using Graduated Gaps and Centrifugal Force2010

    • Author(s)
      H.Maruyama, S.Sakuma, Y.Yamanishi, F.Arai
    • Journal Title

      Journal of Robotics and Mechatronics

      Volume: 22 Pages: 280-285

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The KtrA and KtrE subunits are required for Na^+ dependent K^+ uptake by KtrB across the plasma membrane in Synechocystis sp.PCC 6803.2010

    • Author(s)
      Zulkifli, L., Akai, M., Yoshikawa, A., Shimojima, M., Ohta, H., Guy HR., Uozumi, N.
    • Journal Title

      The Journal of Bacteriology

      Volume: 192 Pages: 5063-5070

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Size-Dependent Filtration and Trapping of Microparticles in a Microfluidic Chip Using Graduated Gaps and Centrifugal Force2010

    • Author(s)
      Hisataka Maruyama, Shinya Sakuma, Yoko Yamanishi, Fumihito Arai
    • Journal Title

      Journal.of Robotics and Mechatronics 22(掲載確定)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Massive Parallel Assembly of Microbeads for Fabrication of Microtools Having Spherical Structure and Powerful Manipulation by Optical Tweezers2010

    • Author(s)
      Hisataka Maruyama, Ryo Iitsuka, Kazuhisa Onda, Fumihito Arai
    • Journal Title

      Journal of Robotics and Mechatronics 22(掲載確定)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Threonine at position 306 of the KAT1 potassium channel is essential for channel activity and is a target site for ABA-activated SnRK2/OST1/SnRK2.6 protein kinase2009

    • Author(s)
      Sato, A., Sato, Y., Fukao, Y., Fujiwara, M.Umezawa, T., Shinozaki, K., Hibi, T., Taniguchi, M, Miyake, H., Goto, D.B., Uozumi, N.
    • Journal Title

      Biochem.J. Vol.22

      Pages: 439-448

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Threonine at position 306 of the KAT1 potassium channel is essential for channel activity and is a target site for ABA-activated SnRK2/OST1/SnRK2.6 protein kinase2009

    • Author(s)
      Sato, A., Sato, Y., Fukao, Y, Fujiwara, M., Umezawa, T., Shinozaki, K., Hibi, T., Taniguchi, M., Miyake, H., Goto, D.B., Uozumi, N.
    • Journal Title

      Biochem.J. 73

      Pages: 439-448

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Massive Parallel Assembly of Microbeads for Fabrication of Microtools Having Spherical Structure and Powerful Laser Manipulation2011

    • Author(s)
      H.Maruyama, R.Iitsuka, K.Onda, F.Arai
    • Organizer
      2010 IEEE International Conference on Robotics and Automation
    • Place of Presentation
      Anchorage, USA
    • Year and Date
      2011-05-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] Evaluation of Thermal Conductivity of Single Carbon Nanotube Using Functional Gel Temperature Sensor in Liquid2011

    • Author(s)
      Kyohei Tomita, Hisataka Maruyama, Fumihito Arai
    • Organizer
      第40回記念フラーレン・ナノチューブ総合シンポジウム
    • Place of Presentation
      名古屋,名城大学
    • Year and Date
      2011-03-10
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Massive Parallel Assembly of Microbeads for Fabrication of Microtools Having Spherical Structure and Powerful Laser Manipulation2010

    • Author(s)
      丸山央峰, 飯塚龍, 恩田一寿, 新井史人
    • Organizer
      2010 IEEE International Conference on Robotics and Automation
    • Place of Presentation
      Anchorage, USA
    • Year and Date
      2010-05-04
    • Related Report
      2010 Final Research Report
  • [Presentation] STZE-DEPENDENT FILTRATION AND TRAP OF MICROPARTICLES USING CENTRIFUGAL FORCE AND GRADUATED MECHANICAL GAP IN MICROFLUIDIC CHIP2009

    • Author(s)
      丸山央峰, 佐久間臣耶, 山西陽子, 新井史人
    • Organizer
      The 13th International Conference on Miniaturized Systems for Chemistry and Life Selenees
    • Place of Presentation
      Korea, JEJU
    • Year and Date
      2009-11-04
    • Related Report
      2010 Final Research Report
  • [Presentation] SIZE-DEPENDENT FILTRATION AND TRAP OF MICROPARTICLES USING CENTRIFUGAL FORCE AND GRADUATED MECHANICAL GAP IN MICROFLUIDIC CHIP2009

    • Author(s)
      Hisataka Maruyama, Shinya Sakuma, Yoko Yamanishi, Fumihito Arai
    • Organizer
      The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    • Place of Presentation
      Korea, JEJU
    • Year and Date
      2009-11-04
    • Related Report
      2009 Annual Research Report
  • [Presentation] Size-Dependent Microparticle Filtration Using Magnetically Driven Microtool for Producing Gel-Microtool2009

    • Author(s)
      Benoit Chapurlat, Hisataka Maruyama, Yoko Yamanishi, Kyosuke Kotani, Fumihito Arai
    • Organizer
      The 2009 IEEE/RSJ International Conference on Intelligent Robots and Systems
    • Place of Presentation
      アメリカ,セントルイス
    • Year and Date
      2009-10-15
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] レーザ駆動Robot-on-a-chipに関する研究-その3:テザード型マイクロツールの設計と製作-2009

    • Author(s)
      猪股直生, 丸山央峰, 新井史人
    • Organizer
      日本機械学会ロボティクス・メカトロニクス講演会2009
    • Place of Presentation
      福岡,福岡国際会議場
    • Year and Date
      2009-05-26
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] レーザ駆動Robot-on-a-chipに関する研究-その1:統合型光ピンセットのバイラテラル制御システム-2009

    • Author(s)
      恩田一寿, 丸山央峰, 新井史人
    • Organizer
      日本機械学会ロボティクス・メカトロニクス講演会2009
    • Place of Presentation
      福岡,福岡国際会議場
    • Year and Date
      2009-05-26
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] 磁気駆動Robot-on-a-chipに関する研究-その3:磁気駆動マイクロツールを用いた微粒子の連続選別-2009

    • Author(s)
      丸山央峰, 佐久間臣耶, シャプラブノア, 山西陽子, 新井史人
    • Organizer
      日本機械学会ロボティクス・メカトロニクス講演会2009
    • Place of Presentation
      福岡,福岡国際会議場
    • Year and Date
      2009-05-26
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] Multi-beam Laser Micromanipulation of Microtool by Integrated Optical Tweezers2009

    • Author(s)
      Fumihito Arai, Kazuhisa Onda, Ryolitsuka, Hisataka Maruyama
    • Organizer
      2009 IEEE International Conference on Robotics and Automation
    • Place of Presentation
      Kobe, Kobe International Conference Center
    • Year and Date
      2009-05-15
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] マイクロ流路を用いたラン藻の高浸透圧ストレスに対する適応機構のリアルタイム観察2009

    • Author(s)
      四十九俊彰
    • Organizer
      日本植物生理学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-21
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] 遠心力と磁気駆動マイクロツールを用いたオンチップ粒子選別2008

    • Author(s)
      丸山央峰
    • Organizer
      第18回化学とマイクロ・ナノシステム研究会
    • Place of Presentation
      京都大学桂キャンパス,船井哲良記念講堂
    • Year and Date
      2008-12-09
    • Related Report
      2010 Final Research Report
  • [Presentation] 遠心力と磁気駆動マイクロツールを用いたオンチップ粒子選別2008

    • Author(s)
      丸山央峰
    • Organizer
      第18回化学とマイクロ・ナノシステム研究会
    • Place of Presentation
      京都大学桂キャンパス, 船井哲良記念講堂
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] Production of Functional Microtool Using Size-Classified Gel-Microbeads2008

    • Author(s)
      Benoit Chapurlat
    • Organizer
      2008 International Symposium on Micro-NanoMechatronics and Human Science
    • Place of Presentation
      名古屋,名古屋大学野依記念学術交流館
    • Year and Date
      2008-11-08
    • Related Report
      2010 Final Research Report
  • [Presentation] Production of Functional Microtool Using Size-Classified Gel-Microbeads2008

    • Author(s)
      Benoit Chapurlat
    • Organizer
      2008 International Symposium on Micro-NanoMechatronics and Human Science
    • Place of Presentation
      名古屋, 名古屋大学野依記念学術交流館
    • Year and Date
      2008-11-08
    • Related Report
      2008 Annual Research Report
  • [Presentation] MTCROPATTERNTNG OF HYDROGEL AND ON-CHIP LONG TIME MONITORING OF INDIVIDLAL CELLS IN A CAGE2008

    • Author(s)
      新井史人
    • Organizer
      The 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    • Place of Presentation
      アメリカ,サンディエゴ
    • Year and Date
      2008-10-15
    • Related Report
      2010 Final Research Report
  • [Presentation] MICROPATTERNING OF HYDROGEL AND ON-CHIP LONG TIME MONITORING OF INDIVIDUAL CELLS IN ACAGE2008

    • Author(s)
      Fumihito Arai
    • Organizer
      The 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    • Place of Presentation
      アメリカ, サンディエゴ
    • Year and Date
      2008-10-15
    • Related Report
      2008 Annual Research Report
  • [Presentation] フォトリソグラフィによるマイクロツールの作製とマルチビーム統合型光ピンセットによる操作2008

    • Author(s)
      飯塚龍
    • Organizer
      第26回日本ロボット学会学術講演会
    • Place of Presentation
      神戸市,神戸大学
    • Year and Date
      2008-09-09
    • Related Report
      2010 Final Research Report
  • [Presentation] フォトリソグラフィによるマイクロツールの作製とマルチビーム統合型光ピンセットによる操作2008

    • Author(s)
      飯塚龍
    • Organizer
      第26回日本ロボット学会学術講演会
    • Place of Presentation
      神戸市, 神戸大学
    • Year and Date
      2008-09-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] マイクロ流路を用いたラン藻の浸透圧適応のリアルタイム観察2008

    • Author(s)
      四十九俊彰
    • Organizer
      日本生物工学会
    • Place of Presentation
      仙台
    • Year and Date
      2008-08-28
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Remarks]

    • URL

      http://www.bioroboties.mech.nagoya-u.ac.jp/research/Robomec09/Robome09_Iitsuka.pdf

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.bioroboties.mech.nagoya-u.ac.jp/research/Robomec09/Robomec09_Maruyama.pdf

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.imech.mech.tohoku.ac.jp/research/Robomec09/Robomec09_Onda.pdf

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.imech.mech.tohoku.ac.jp/research/Robomec09/Robomec09_Inomata.pdf

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.bioroboties.mech.nagoya-u.ac.jp/research/40th_FNTS/40th_FNTS_Tomita.pdf

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.biorobotics.mech.nagoya-u.ac.jp/research/Robomec09/Robomec09_Iitsuka.pdf

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.biorobotics.mech.nagoya-u.ac.jp/research/Robomec09/Robomec09_Maruyama.pdf

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.biorobotics.mech.nagoya-u.ac.jp/research/40th_FNTS/40th_FNTS_Tomita.pdf

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.imech.mech.tohoku.ac.jp/research/Robomec09/Robomec09_Onda.pdf

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.imech.mech.tohoku.ac.jp/research/Robomec09/Robomec09_Inomata.pdf

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.imech.mech.tohoku.ac.jp/research/Robomec09/Robomec09_Maruyama.pdf

    • Related Report
      2009 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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