Analysis of phloem function as a signaling center
Project/Area Number |
20247003
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | The University of Tokyo |
Principal Investigator |
FUKUDA Hiroo The University of Tokyo, 大学院・理学系研究科, 教授 (10165293)
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Project Period (FY) |
2008 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥49,140,000 (Direct Cost: ¥37,800,000、Indirect Cost: ¥11,340,000)
Fiscal Year 2010: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2009: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2008: ¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
|
Keywords | 成長生理 / 植物 / 発生・分化 / 師部 / 維管束 / 細胞間シグナル / CLEペプチド / ブラシノステロイド / オーキシン / 形成層 / 受容体 / TDIF / シロイヌナズナ / ヒャクニチソウ / アレイ解析 |
Research Abstract |
Plant body plan is regulated by the tight network of cell-cell communication. Vascular plants possess a unique long-distance transport system of nutrients and signals, named the vascular system. The vascular system consists of xylem, phloem and procambium/cambium, which gives rise to xylem and phloem cells. We have identified TDIF, a dodeca-peptide that regulates vascular development as an intercellular signal secreted from phloem. In this study, therefore, we analyzed the function of phloem-related signals involved in vascular development. For this purpose we focused on the following three themes. 1) To elucidate the function of TDIF as a phloem signal and consequently to understand of the status of phloem. 2) To analyze the function of COE1 receptor kinase, whose gene may be expressed in phloem cells and function in vascular pattern formation of rice. 3) To identify new transcription factors that regulate TDIF expression in phloem. As a result, we succeeded in demonstrating that phloem cells functions as niche cells in the maintenance of vascular stem cells such as procambial and cambial cells by providing TDIF as a intercellular factor promoting self-renewal of stem cells and inhibiting xylem cell differentiation. We also indicated that COE1 functions in spatial regulation of vascular tissue differentiation in concert with auxin and brassinosteroid signals. Finally we isolated transcription factors regulating the CLE41 gene encoding TDIF, using a new comprehensive transcription factor screening method. These findings casted a new light on function of the phloem cells as a signal center.
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Report
(4 results)
Research Products
(144 results)
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[Journal Article] A receptor-like kinase, KLAVIER, mediates systemic regulation of nodulation and non-symbiotic shoot development in Lotus japonicus2011
Author(s)
Miyazawa H., Oka-Kira E., Sato N., Takahashi H., Wu G. J., Sato S., Hayashi M., Betsuyaku S., Nakazono M., Tabata S., Harada Y., Sawa S., Fukuda H., Kawaguchi M.
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[Journal Article] Mitogen-Activated Protein Kinase regulated by the CLAVATA receptors contributes to the shoot apical meristem homeostasis.2011
Author(s)
Betsuyaku, S., Takahashi, F., Kinoshita, A., Miwa, H., Shinozaki, K., Fukuda, H., Sawa, S.
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Journal Title
Plant Cell Physiol.
Volume: 52
Pages: 14-29
NAID
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[Journal Article] ANGUSTIFOLIA3 plays roles in adaxial/abaxial patterning and growth in leaf morphogenesis.2011
Author(s)
Horiguchi, G., Nakayama, H., Ishikawa N., Kubo, M., Demura, T., Fukuda, H., Tsukaya, H.
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Journal Title
Plant Cell Physiol.
Volume: 52
Pages: 112-124
NAID
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[Journal Article] Areceptor-like kinase, KLAVIER, mediates systemic regulation of modulation and non-symbiotic shoot development in Lotus japonicus.2011
Author(s)
Miyazawa, H., Oka-Kira, E., Sato, N., Takahashi, H., Wu, G.J., Sato, S., Hayashi, M., Betsuyaku, S., Nakazono, M., Tabata, S., Harada, Y., Sawa, S., Fukuda, H., Kawaguchi, M.
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Journal Title
Development
Volume: (in press)(掲載確定)
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[Journal Article] CKHI/EER4 gene encoding a TAF12-like protein negatively regulates cytokinin sensitivity in Arabnidopsis thaliana.2011
Author(s)
Kubo, M., Furuta, K., Demura, T., Fukuda, F., Liu, Y.-G., Shibata, S., Kakimoto, T.
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Journal Title
Plant Cell Physiol.
Volume: (in press)(掲載確定)
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[Journal Article] ADP-ribosylation factor (ARF), guanine nucleotide exchange factor (GEF), and GAPase-acticating protein (GAP) function in endocytosis of plant cells2010
Author(s)
Naramoto S., Kleine-Vehn J., Robert S., Fujimoto M., Dainobu T., Paciorek T., Ueda T., Nakano A., Van Montagu M. C. E., Fukuda H., Friml J.
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Journal Title
Proc.Natl.Acad.Sci.,USA 107
Pages: 21890-21895
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[Journal Article] VND-INTERACTING2, a NAC Domain Protein, Negatively Regulates Xylem Vessel Formation in Arabidopsis.2010
Author(s)
Yamaguchi, M., Ohtania, M., Mitsuda, N., Kubo, M., Ohme-Takagi, M., Fukuda, H., Demura, T.
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Journal Title
Plant Cell
Volume: 22
Pages: 1249-1263
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[Journal Article] COE1, an LRR-RLK responsible for commissral vein pattern formation in rice.2010
Author(s)
Sakaguchi, J., Itoh, J.-I., Ito, Y., Nakamura, A., Fukuda, H., Sawa, S.
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Journal Title
Plant Journal
Volume: 63
Pages: 405-416
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[Journal Article] RPK2/TOAD2 is an essential receptor-like kinase transmitting the CLV3 signal in Arabidopsis.2010
Author(s)
Kinoshita, A., Betsuyaku, S., Osakabe, Y., Mizuno, S., Nagawa, S., Stahl, Y., Simon, R., Yamaguchi-Shinozaki, K., Fukuda, H., Sawa, S.
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Journal Title
Development
Volume: 137
Pages: 3911-3920
NAID
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[Journal Article] ADP-ribosylation factor machinery mediates endocytosis in plant cells.2010
Author(s)
Naramoto, S., Kleine-Vehn, J., Robert, S., Fujimoto, M., Dainobu, T., Paciorek, T., Ueda, T., Nakano, A., Van Montagu, M.C.E., Fukuda, H., Friml, J.
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Journal Title
Proc.Natl.Acad.Sci., USA
Volume: 107
Pages: 21890-21895
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[Journal Article] Preferential up-regulation of G2/M phase-specific genes by overexpressi on of hyperactive form of NtmybA2 lacking its negative regulation domain in tobacco BY2 cells.2009
Author(s)
Kato, K., Galis, I., Suzuki, S, Araki, S., Demura, T., Criqui, M.-C., Potuschak, T., Genschik, P., Fukuda, H., Matsuoka, K., Ito, M.
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Journal Title
Plant Physiol.
Volume: 149
Pages: 1945-1957
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[Journal Article] Involvement of phytosulfokine in the attenuation of stress response during the transdifferentiation of Zinnia mesophyll cells into tracheary elements.2009
Author(s)
Motose, H., Iwamoto, K., Endo, S., Demura, T., Sakagami, Y., Matsubayashi, Y., Moore, K. L., Fukuda, H.
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Journal Title
Plant Physiol.
Volume: 150
Pages: 437-447
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Peer Reviewed
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[Journal Article] Phosphoinositide-dependent regulation of VAN3 ARF-GAP localization and activity essential for vascular tissue continuity in plants.2009
Author(s)
Naramoto, S., Sawa, S., Koizumi, K., Uemura, T., Ueda, T., Friml, J., Nakano, A., Fukuda, H.
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Journal Title
Development
Volume: 136
Pages: 1529-1538
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[Journal Article] Identifying new components participating in the secondary cell wall for mation of vessel elements in Zinnia and Arabidopsis.2009
Author(s)
Endo, S., Pesquet, E., Yamaguchi, M., Tashiro, G., Sato, M., Toyooka, K., Nishikubo, N., Motose-U., M., Kubo, M., Fukuda, H., Demura, T.
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Journal Title
Plant Cell
Volume: 21
Pages: 1155-1165
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Peer Reviewed
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[Journal Article] Functional analyses of the Arabidopsis LONELY GUY family encoding cytokinin-activating enzymes reveal the importance of the direct activati on pathway in Arabidopsis thaliana.2009
Author(s)
Kuroha, T., Tokunaga, H., Kojima, M., Ueda, N., Ishida, T., Nagawa, S., Fukuda, H., Sugimoto-Shirasu, K., Sakakibara, H.
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Journal Title
Plant Cell
Volume: 21
Pages: 3152-3169
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[Journal Article] Ecogenomics of cleistogamous and chasmogamous flowering: genome-wide gene expression patterns from cross-species microarray analysis in Cardamine kokaiensis (Brassicaceae).2008
Author(s)
Morinaga, S.-I., Nagano, A.J. Miyazaki, S., Kubo, M., Demura, T., Fukuda, H., Sakai, S. and Hasebe, M.
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Journal Title
J. Ecol. 96
Pages: 1086-1097
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[Journal Article] Non-cell-autOnomous control of vascular stem cell fates by a CLE peptide/receptor system.2008
Author(s)
Hirakawa Y., Shinohara, H., Kondo, Y., Inoue, A., Nakanomyo, I., Ogawa, M., Sawa, S., Ohashi-Ito, K., Matsubayashi, Y. and Fukuda, H.
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Journal Title
Proc. Natl. Acad. Sci. USA 105
Pages: 15208-15213
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[Journal Article] The receptor-like kinase SOL2 mediates CLE signaling in Arabidopsis.2008
Author(s)
Miwa, H., Betsuyaku, S., Iwamoto, K., Kinoshita, A., Fukuda H., and Sawa, S.
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Journal Title
Plant Cell Physiol. 49
Pages: 1752-1757
NAID
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[Presentation] Regulation of auxin transport dependent leaf vascular continuity by VAN3 ARF-GAP.2010
Author(s)
Naramoto, S, Koizumi, K., Ueda, T., Nakano, A., Fukuda, H., Friml, J.
Organizer
21st International Conference on Arabidopsis Research
Place of Presentation
Yokohama, Japan
Related Report
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[Presentation] Molecular dissection of CLV3 signaling.2010
Author(s)
Betsuyaku, S., Kinoshita, A., Fujiwara, M., Fukao, Y., Kuromori, T., Shinozaki, K., Suzuki, A., Takahashi, H., Fukuda, H., Sawa, S.
Organizer
21st International Conference on Arabidopsis Research
Place of Presentation
Yokohama, Japan
Related Report
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[Presentation] Genetic analysis of the CLV signaling pathway in the SAM.2010
Author(s)
Kinoshita, A., Betsuyaku, S., Yamada, M., Fukuda, H., Sawa, S.
Organizer
21st International Conference on Arabidopsis Research
Place of Presentation
Yokohama, Japan
Related Report
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[Presentation] VND-INTERACTING2, a NAC domain transcription factor, negatively regulates xylem vessel formation in Arabidopsis.2010
Author(s)
Yamaguchi, M., Ohtani, M., Mitsuda, N., Kubo, M.Ohme-Takagi, M., Fukuda, H., Demura, T.
Organizer
21st International Conference on Arabidopsis Research
Place of Presentation
Yokohama, Japan
Related Report
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[Presentation] COE1, an LRR-RLK responsible for commissural vein pattern formation in rice.2010
Author(s)
Sakaguchi, J., Itoh, J.-I., Ito, Y., Nakamura, A., Sawa, S., Fukuda, H.
Organizer
Cold Spring Harbor Asia Symposium "From Plant Biology to Crop Biotechnology"
Place of Presentation
CSH Asia, Suzhou, China
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[Presentation] Molecular dissection of CLV3 peptide signaling.2008
Author(s)
Betsuyaku, S., Kuromori, T., Shinozaki, K., Suzuki, A., Takahashi, H., Fukuda, H., and Sawa, S.
Organizer
第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
Place of Presentation
神戸ポートアイランド
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[Presentation] SOL2 mediates CLE signaling pathway in Arabidopsis.2008
Author(s)
Miwa, H., Betsuyaku, S., Iwamoto, K., Kinoshita, A., Fukuda, H. and Sawa S.
Organizer
19th International Conference on Arabidopsis Research
Place of Presentation
Montreal, Canada
Related Report
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[Presentation] Molecular dissection of CLV3 peptide signalling. 19th International Conference on Arabidopsis Research2008
Author(s)
Betsuyaku, S., Kuromori, T., Shinozaki, K., Suzuki, A., Takahashi, H., Fukuda H. and Sawa, S.
Organizer
19th International Conference on Arabidopsis Research
Place of Presentation
Montreal, Canada
Related Report
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[Presentation] Molecular Dissection of CLV3 Peptide Signalling.2008
Author(s)
Betsuyaku, S., Miwa, H., Kuromori, T., Shinozaki, K., Suzuki, A., Takahashi, Hi., Fukuda H. and Sawa S.
Organizer
Frontiers of Plant Science in the 21st Century
Place of Presentation
Okazaki
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