| Budget Amount *help |
¥43,030,000 (Direct Cost: ¥33,100,000、Indirect Cost: ¥9,930,000)
Fiscal Year 2011: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2010: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2009: ¥10,400,000 (Direct Cost: ¥8,000,000、Indirect Cost: ¥2,400,000)
Fiscal Year 2008: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
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| Research Abstract |
Using the proteins from M.musculus (mRAT) and from S.cerevisiae RAT (yRAT), it has been directly proved that a eucaryotic homolog of E. coli tRNA acetyltransferase TmcA (RAT) has the activity of transferring the acetyl group to the specific site of 18S rRNA. It has also been proved that these proteins have the ATPase activity. As a result of crystallization screening, we found that the crystals of RAT from S.cerevisiae of which C-terminal 123 residues are truncated (deltaC123-yRAT) diffract X-rays to 9 A resolution at BL32XU, SPring-8.
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