| Project/Area Number |
20247009
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
MIKI Kunio 京都大学, 大学院・理学研究科, 教授 (10116105)
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| Co-Investigator(Renkei-kenkyūsha) |
ATOMI Haruyuki 京都大学, 大学院・工学研究科, 教授 (90243047)
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| Research Collaborator |
WATANABE Satoshi 京都大学, 大学院・理学研究科, 特定研究員 (50432357)
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| Project Period (FY) |
2008 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000)
Fiscal Year 2010: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2009: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Keywords | X線結晶解析 / タンパク質の構造機能解析 / 金属取り込み / ヒドロゲナーゼ / hypタンパク質 / 複合体構造 / Hypタンパク質 |
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| Research Abstract |
Six Hyp proteins(HypA, B, C, D, E, and F) are primarily involved in the maturation process of[NiFe] hydrogenases which catalyze reversible hydrogen production and consumption. These proteins incorporate a complex Ni/Fe cluster into a protein subunit of[NiFe] hydrogenases. On the basis of our success in the first structure determination of HypC, HypD, and HypE, we have carried out here structure an alyses of HypA, HypB, and HypF and of complexes between HypC, HypD, and HypE to obtain mechanistic insights into the[NiFe] hydrogenase maturation.
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