Regulation of intracellular vesicle transport by small GTPase cycles

• Project/Area Number: 20247011
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: KATADA Toshiaki The University of Tokyo, 大学院・薬学系研究科, 教授 (10088859)
• Co-Investigator(Kenkyū-buntansha): KONTANI Kenji 東京大学, 大学院・薬学系研究科, 准教授 (30302615) ; KAJIHO Hiroaki 東京大学, 大学院・薬学系研究科, 助教 (70401221) ; FUKUYAMA Masamitsu 東京大学, 大学院・薬学系研究科, 助教 (20422389) ; KOBAYASHI Tetsuo 東京大学, 大学院・薬学系研究科, 助教 (80433994)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥42,900,000 (Direct Cost: ¥33,000,000、Indirect Cost: ¥9,900,000); Fiscal Year 2010: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000); Fiscal Year 2009: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000); Fiscal Year 2008: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
• Keywords: タンパク質 / 遺伝子 / シグナル伝達 / G蛋白質 / 小胞輸送 / リソソーム / Gサイクル / ゲノムプロジェクト / Processing body

Research Abstract

G proteins, which cycle between the two different GTP- and GDP-bound conformations (G cycles), play important roles as a "molecular switch" in many intracellular signaling pathways. In the Grant-in-Aid for Scientific Research (A), we investigated how G cycles are regulated dependent on their modes of actions in intracellular vesicle transport and found that novel G proteins containing unique biochemical properties and/or structures are involved in the regulation of endosome dynamics, such as lysosome biogenesis, endocytosis, and exocytosis.

Research Products

• [Journal Article] Characterization of RIN3 as a guanine-nucleotide exchange factor for the Rab5-subfamily GTPase Rab31. (2011)
  • Author(s): Kajiho H, Sakurai, K, Minoda T, Yoshikawa M, Nakagawa S, Fukushima S, Kontani K, Katada T
  • Journal Title: J.Biol.Chem.
• [Journal Article] cTAGE5 mediates collagen secretion through interaction with TANGO1 at endoplasmic reticulum exit sites (2011)
  • Author(s): Saito K, Yamashiro K, Ichikawa Y, Erlmann P, Kontani K, Malhotra V, Katada T
  • Journal Title: Mol.Biol.Cell.
• [Journal Article] RASSF7 negatively regulates pro-apoptotic JNK signaling by inhibiting the activity of phosphorylated-MKK7 (2011)
  • Author(s): Takahashi S, Ebihara A, Kajiho H, Kontani K, Nishina H, Katada T
  • Journal Title: Cell Death Differ 18 Pages: 645-655
• [Journal Article] RhoA activation participates in rearrangement of processing bodies and release of nucleated AU-rich mRNAs (2011)
  • Author(s): Takahashi S, Sakurai K, Ebihara A, Kajiho H, Saito K, Kontani K, Nishina H, Katada T
  • Journal Title: Nucleic Acids Res 39 Pages: 3446-3457
• [Journal Article] RhoA activation participates in rearrangement of processing bodies and release of nucleated AU-rich mRNAs. (2011)
  • Author(s): Takahashi S, et al.
  • Journal Title: Nucleic Acids Res. Volume: 39 in press(掲載確定)
  • Peer Reviewed
• [Journal Article] The Arf-like GTPase Arl8 mediates delivery of endocytosed macromolecules to lysosomes in Caenorhabditis elegans (2010)
  • Author(s): Nakae I, Fujino T, Kobayashi T, Sasaki A, Kikko Y, Fukuyama M, Gengyo-Ando K, Mitani S, Kontani K, Katada T
  • Journal Title: Mol.Biol.Cell 21 Pages: 2434-2442
• [Journal Article] Joubert syndrome Arl13b functions at ciliary membranes and stabilizes protein transport in Caenorhabditis elegans (2010)
  • Author(s): Cevik S, Hori Y, Kaplan OI, Kida K, Toivenon T, Foley-Fisher C, Cottell D, Katada T, Kontani K, Blacque OE
  • Journal Title: J.Cell Biol. 188 Pages: 953-969
• [Journal Article] Quantitative characterization of Tob interactions provides the thermodynamic basis for translation termination-coupled deadenylase regulation (2010)
  • Author(s): Ruan L, Osawa M, Hosoda N, Imai S, Machiyama A, Katada T, Hoshino S, Shimada I
  • Journal Title: J.Biol.Chem. 285 Pages: 27624-27631
• [Journal Article] An Arf-like small G protein, ARL-8, promotes the axonal transport of presynaptic cargoes by suppressing vesicle aggregation (2010)
  • Author(s): Klassen MP, Wu YE, Maeder CI, Nakae I, Cueva JG, Lehrman EK, Tada M, Gengyo-Ando K, Wang GJ, Goodman M, Mitani S, Kontani K, Katada T, Shen K
  • Journal Title: Neuron 66 Pages: 710-723
• [Journal Article] Gタンパク質研究の動向 (2010)
  • Author(s): 堅田利明
  • Journal Title: 細胞(The Cell) 42(3) Pages: 90-91
• [Journal Article] アミノ酸シグナル伝達に介在するGタンパク質Rag (2010)
  • Author(s): 春日秀文、福山征光、紺谷圏二、堅田利明
  • Journal Title: 細胞(The Cell) 42(3) Pages: 108-111
• [Journal Article] The Arf-like GTPase Arl8 mediates delivery of endocytosed macromolecules to lysosomes in Caenorhabditis elegans. (2010)
  • Author(s): Nakae I, et al.
  • Journal Title: Mol.Biol.Cell Volume: 21 Pages: 2434-2442
  • Peer Reviewed
• [Journal Article] An Arf-like small G protein, ARL-8, promotes the axonal transport of presynaptic cargoes by suppressing vesicle aggregation. (2010)
  • Author(s): Klassen MP, et al.
  • Journal Title: Neuron Volume: 66 Pages: 710-723
  • Peer Reviewed
• [Journal Article] Joubert syndrome Arl13b functions at ciliary membranes and stabilizes protein transport in Caenorhabditis elegans. (2010)
  • Author(s): Cevik S, et al.
  • Journal Title: J.Cell Biol. Volume: 188 Pages: 953-969
  • Peer Reviewed
• [Journal Article] Gタンパク質研究の動向 (2010)
  • Author(s): 堅田利明
  • Journal Title: 細胞(The Cell) Volume: 42(3) Pages: 90-91
• [Journal Article] The Arf-like GTPase Ar18 mediates delivery of endocytosed macromolecules to lysosomes in Caenorhabditis elegans. (2010)
  • Author(s): Nakae I, et al.
  • Journal Title: Mol.Biol.Cell Volume: 21 Pages: 2434-2442
  • Peer Reviewed
• [Journal Article] Arf-like protein 13B (2009)
  • Author(s): Kontani K, Hori Y, Katada T
  • Journal Title: Nature Molecule Pages
• [Journal Article] Biochemical characterization of missense mutations in the Arf/Arl-family small GTPase Arl6 causing Bardet-Biedl syndrome (2009)
  • Author(s): Kobayashi T, Hori Y, Ueda N, Kajiho H, Muraoka S, Shima F, Kataoka T, Kontani K, Katada T
  • Journal Title: Biochem.Biophys.Res.Commun. 381(3) Pages: 439-442
• [Journal Article] Biochemical characterization of missense mutations in the Arf/Arl-family small GTPase Ar16 causing Bardet-Biedl syndrome (2009)
  • Author(s): Kobayashi T., et al.
  • Journal Title: Biochem.Biophys.Res.Commun. 381 Pages: 439-442
  • Peer Reviewed
• [Journal Article] Upf1 potentially serves as a RING-related E3 ubiquitin ligase via its association with Upf3 in yeast (2008)
  • Author(s): Takahashi S, Araki Y, Ohya Y, Sakuno T, Hoshino S, Kontani K, Nishina H, Katada T
  • Journal Title: RNA 14 Pages: 1950-1958
• [Journal Article] Domain architecture of the atypical Arf-family GTPase Arl13b involved in cilia formation (2008)
  • Author(s): Hori Y, Kobayashi T, Kikko Y, Kontani K, Katada T
  • Journal Title: Biochem.Biophys.Res.Commun. 373 Pages: 119-124
• [Journal Article] Tyr-phosphorylation signals translocate RIN3, the small GTPase Rab5-GEF, to early endocytic vesicles (2008)
  • Author(s): Yoshikawa M, Kajiho H, Sakurai K, Minoda T, Nakagawa S, Kontani K, Katada T
  • Journal Title: Biochem.Biophys.Res.Commun. 372 Pages: 168-172
• [Journal Article] Tyr-phosphorylation signals translocate RIN3, the small GTPase Rab5-GEF, to early endocytic vesicles (2008)
  • Author(s): Yoshikawa M., et al.
  • Journal Title: Biochem.Biophys.Res.Commun. 372 Pages: 168-172
  • Peer Reviewed
• [Journal Article] Domain architecture of the atypical Arf-family GTPase Arll3b involved in cilia formation (2008)
  • Author(s): Hori Y., et al.
  • Journal Title: Biochem.Biophys.Res.Commun. 373 Pages: 119-124
  • Peer Reviewed
• [Journal Article] Upf1 potentially serves as a RING-related E3 ubiquitin ligase via its association with Upf3 in yeast (2008)
  • Author(s): Takahashi S., et al.
  • Journal Title: RNA 14 Pages: 1950-1958
  • Peer Reviewed
• [Presentation] 後期エンドソームとリソソームの融合・再形成過程に介在する低分子量G蛋白質Ar18 (2009)
  • Author(s): 堅田利明、中江郁青、菊香順史、紺谷圏二
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸(シンポジウム講演)
  • Year and Date: 2009-10-23
• [Presentation] The Arf-like GTPase Ar18 is required for lysosome biogenesis in C.elegans (2009)
  • Author(s): 堅田利明, 紺谷圏二
  • Organizer: IUPS 2009 : PSJ Symposium, VI-5 ; Recent progress on G-protein signalings
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2009-07-28
• [Presentation] The Arf-like GTPase Ar18 is required for lysosome biogenesis in C.elegans. (2009)
  • Author(s): Katada T., et al.
  • Organizer: 国際生理学会IUPS2009シンポジウム
  • Place of Presentation: 京都・京都国際会館
  • Year and Date: 2009-07-28
• [Presentation] The Arf-like GTPase Arl8 is required for lysosome biogenesis in C.elegans (2009)
  • Author(s): Katada T., et al.
  • Organizer: 国際生理学会IUPS2009シンポジウム
  • Place of Presentation: 京都・京都国際会館
  • Year and Date: 2009-07-28
• [Presentation] The Arf-like GTPase ARL-8 is required for lysosome biogenesis in C. elegans (2009)
  • Author(s): Nakae I, Fujino T, Kobayashi T, Kikko Y, Harada S, Fukuyama M, Gengyo-Ando K, Mitani S, Kontani K, Katada T
  • Organizer: 17th International C.elegans Meeting
  • Place of Presentation: Los Angeles, CA, USA
  • Year and Date: 2009-06-27
• [Presentation] Distinct control of survival, and somatic and germline development during L1 diapause by the insulin/IGF signaling and AMPK pathway (2009)
  • Author(s): Fukuyama M, Sakuma K, Astumi Y, Shimomura H, Kasuga H, Rougvie A, Katada T
  • Organizer: 17th International C.elegans Meeting
  • Place of Presentation: Los Angeles, CA, USA
  • Year and Date: 2009-06-26
• [Presentation] Tyr-phosphorylation signals translocate RIN3, the small GTPase Rab5-GEF, to early endocytic vesicles (2008)
  • Author(s): Kajiho H, Yoshikawa M, Sakurai K, Minoda T, Nakagawa S, Kontani K, Katada T
  • Organizer: 48th ASCB Annual Meeting
  • Place of Presentation: 米国、サンフランシスコ
• [Presentation] 低分子量G蛋白質Ar18の神経細胞における機能解析 (2008)
  • Author(s): 中江郁青、安藤恵子、三谷昌平、紺谷圏二、堅田利明
  • Organizer: ファーマ・バイオフォーラム2008
  • Place of Presentation: 東京【優秀発表賞】(口頭発表)
• [Presentation] 低分子量G蛋白質Rhoファミリーは新規細胞内構造体P-bodyの形成を制御する (2008)
  • Author(s): 櫻井京子、高橋真也、蛯原有沙、梶保博昭、仁科博史、堅田利明
  • Organizer: 文部科学省特定領域研究「細胞情報ネットワークを統合するG蛋白質シグナル研究の新展開」班会議
  • Place of Presentation: 湯沢【最優秀発表賞】(ポスター発表)
• [Presentation] 細胞のシグナル伝達系に介在するGタンパク質:三量体Gタンパク質Giの発見からGタンパク質が果たす役割の拡大に向けて (2008)
  • Author(s): 堅田利明
  • Organizer: 産業医科大学大学院イニシアティブワークショップ2008
  • Place of Presentation: 北九州(特別講演)
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