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Structural and functional dynamics of the KcsA channel : Single molecule measurements of the conformational change during gating.

Research Project

Project/Area Number 20247016
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionUniversity of Fukui

Principal Investigator

OIKI Shigetoshi  University of Fukui, 医学部, 教授 (10185176)

Co-Investigator(Kenkyū-buntansha) IWAMOTO Masayuki  福井大学, 医学部, 助教 (40452122)
SHIMIZU Hirofumi  福井大学, 医学部, 講師 (50324158)
KONNO Takashi  福井大学, 医学部, 准教授 (50225637)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥44,200,000 (Direct Cost: ¥34,000,000、Indirect Cost: ¥10,200,000)
Fiscal Year 2010: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2009: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
Fiscal Year 2008: ¥25,480,000 (Direct Cost: ¥19,600,000、Indirect Cost: ¥5,880,000)
Keywordsチャネル / 構造変化 / ゲーティング / 構造機能相関 / 一分子計測 / イオンチャネル / 1分子測定 / 高輝度放射光 / X線回折 / 単一チャネル電流記録
Research Abstract

To detect conformational changes of ion channels upon gating, the diffracted X-ray tracking method has been established. We improved the method to trace fine movements of the diffraction spots with the high spatial and temporal resolutions. For the high flux white X-ray source, we used two synchrotron facilities (ESRF [European Synchrotron Radiation Facility, Grenoble, France] ; SLS [Swiss Light Source, Villigen, Switzerland]). By optimizing the spectrum of white X-rays with the aid of the beam scientists, the background noise was reduced substantially, and we attained the high speed recording as high as 5000 frame/s. Under these experimental setups, the conformational changes of the KcsA potassium channel upon opening and closing of the gate were recorded in the single molecule level. The KcsA channel is a pH-sensitive channel, and by jumping the solution pH from neutral to acidic, the initial gating processes were expected to be recorded. The pH jump was attained by utilizing a caged proton, to which the UV lazer of 355 nm was irradiated. The time course of the pH jump in the chamber was measured and it was revealed that the pH jump was completed within 100 ms. The conformational changes before and after the pH jump differed significantly. These video data were under the image processing for detecting the diffraction spots and tracing of the trajectories on the series of the frames. Automatic retrieval of the coordinate of the spots is the goal of this program.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (63 results)

All 2011 2010 2009 2008 2005 Other

All Journal Article (24 results) (of which Peer Reviewed: 22 results) Presentation (27 results) Book (8 results) Remarks (2 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Cycle Flux Algebra for Ion and Water Flux through the KcsA Channel Single-file Pore Links Microscopic Trajectories and Macroscopic Observables.2011

    • Author(s)
      Oiki S., M.Iwamoto, T.Sumikama
    • Journal Title

      PLoS ONE 6

    • NAID

      120003072326

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cycle Flux Algebra for Ion and Water Flux through the KcsA Channel Single-file Pore Links Microscopic Trajectories and Macroscopic Observables.2011

    • Author(s)
      Oiki, S., M.Iwamoto, T.Sumikama
    • Journal Title

      PLos ONE

      Volume: 6

    • NAID

      120003072326

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic structure of the polytheonamide B channel studiedby normal mode analysis.2011

    • Author(s)
      Mori, T., Kokubo, H., Oiki, S, Okamoto Y.
    • Journal Title

      Molec Srm

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single Molecular Gating Dynamics for KcsA Potassium Channel.2011

    • Author(s)
      Oiki, S., H.Shimizu, M.Iwamoto, T.Konno
    • Journal Title

      Adv.Chem.Phys.

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] イオンチャネルタンパク質の構造変化を追う2011

    • Author(s)
      老木成稔
    • Journal Title

      パリティ

      Volume: 26 Pages: 52-56

    • Related Report
      2010 Annual Research Report
  • [Journal Article] A cytotoxic peptide from a marine sponge exhibits ion channel activity through vectorial-insertion into the membrane.2010

    • Author(s)
      Iwamoto, M., H.Shimizu, I.Muramatsu, S.Oiki
    • Journal Title

      FEBS Lett. 584

      Pages: 3995-3999

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Mesoscopic Approach to Understanding the Mechanisms Underlying the Ion Permeation on the Discrete-State Diagram.2010

    • Author(s)
      Oiki S., M.Iwamoto, T.Sumikama
    • Journal Title

      J.Gen.Physiol. 136

      Pages: 363-365

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Mesoscopic Approach to Understanding the Mechanisms Underlving the Ion Permeation on the Discrete-State Diagram.2010

    • Author(s)
      Oiki, S., M.Iwamoto, T.Sumikama
    • Journal Title

      J.Gen.Physiol.

      Volume: 136 Pages: 363-365

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A cytotoxic peptide from a marine sponge exhibits ion channeI activity through vectorial-insertion into the membrane.2010

    • Author(s)
      Iwamoto, M., H.Shimizu, I.Muramatsu, S.Oiki
    • Journal Title

      FEBS Lett.

      Volume: 584 Pages: 3995-3999

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] KcsAチャネル蛋白質の1分子測定による構造ゆらぎと機能ゆらぎ2010

    • Author(s)
      老木成稔
    • Journal Title

      Medical Bio

      Volume: 10別冊 Pages: 55-60

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Single Molecular Gating Dynamics for KcsA Potassium Channel2010

    • Author(s)
      Oiki, S., H.Shimizu, M.Iwamoto, T.Konno
    • Journal Title

      Adv.Chem.Phys. (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Temperature dependence of Proton Permeation through a Voltage-Gated Proton Channel.2009

    • Author(s)
      Kuno, M., H.Ando, H.Morihata, H.Sakai, H.Mori, M.Sawada, S.Oiki
    • Journal Title

      J.Gen.Physiol. 134

      Pages: 191-205

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Docking Model of Drug Binding to the Human Ether-a-go-go Potassium Channel Guided by Tandem Dimer Mutant Patch-Clamp Data : A SvnerLic Annroach.2009

    • Author(s)
      Imai, Y.N., S.Ryu, S.Oiki
    • Journal Title

      J.Med.Chem. 52

      Pages: 1630-1638

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] X線一分子追跡法により明らかになったKcsAカリウムチャネルの開閉機構.2009

    • Author(s)
      老木成稔
    • Journal Title

      放射光 22

      Pages: 183-191

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Docking Model of Drug Binding to the Human Ether-a-go-go Potassium Channel Guided by Tandem Dimer Mutant Patch-Clamp Data : A Synergic Approach2009

    • Author(s)
      Imai, Y.N., S.Ryu, S.Oiki
    • Journal Title

      J.Med.Chem. 52

      Pages: 1630-1638

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Temperature dependence of Proton Permeation through a Voltage-Gated Proton Channel2009

    • Author(s)
      Kuno, M., H.Ando, H.Morihata, H.Sakai, H.Mori, M.Sawada, S.Oiki
    • Journal Title

      J.Gen.Physiol. 134

      Pages: 191-205

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] X線一分子追跡法により明らかになったKcsAカリウムチャネルの開閉機構2009

    • Author(s)
      老木成稔
    • Journal Title

      放射光 22

      Pages: 183-191

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Docking model of drug binding to the human ether-a-go-go potassium channel guided by tandem dimer mutant patch-clamp data : a synergic approach.2009

    • Author(s)
      Imai YN, Ryu S, Oiki S.
    • Journal Title

      J. Med. Chem. 52

      Pages: 1630-1638

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Topological Mapping of the Asymmetric Drug Binding to the HERG Potassium Channel By Use of Tandem Dimers.2008

    • Author(s)
      Myokai, T., Ryu, S., Shimizu, H., Olkl, S.
    • Journal Title

      MoL Pharmacol. 73

      Pages: 1643-1651

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Global Twisting Motion of Single Molecular KcsA Potassium Channel Upon Gating.2008

    • Author(s)
      Shimizu, H., Iwamoto, M., Konno, T., Nihei, A., Sasaki, Y.C., Oiki S.
    • Journal Title

      Cell 132

      Pages: 67-78

    • NAID

      120000807670

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Topological mapping of the asymmetric drug binding to the humanether-a-go-go-related gene product (HERG) potassium channel by use of tandem dimers.2008

    • Author(s)
      Myokai T, Ryu S, Shimizu H, Oiki S.
    • Journal Title

      Mol. Pharmacol. 73

      Pages: 1643-1651

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Kチャネルのイオン透過機構 : 新しい流動電位測定法により明らかになったイオンー水流束比2008

    • Author(s)
      老木成稔
    • Journal Title

      生物物理 48

      Pages: 246-252

    • NAID

      110006839208

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Single Molecular Gating Dynamics for KcsA Potassium Channel.

    • Author(s)
      Oiki S., H.Shimizu, M.Iwamoto, T.Konno
    • Journal Title

      Adv.Chem.Phys. In press.

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic structure of the polytheonamide B channel studied by normal mode analysis.

    • Author(s)
      Mori, T., Kokubo, H., Oiki S., Okamoto Y.
    • Journal Title

      Molec Sim In press.

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] FLUCUATION OF ION AND WATER FLUX THROUGH THE KcsA POTASSIUM CHANNEL.2010

    • Author(s)
      S.Oiki, M.Iwamoto, T.Sumikama
    • Organizer
      新学術領域研究「揺らぎと生体機能」平成22年度第4回公開シンポジウム
    • Place of Presentation
      大津市
    • Year and Date
      2010-11-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] 新奇異常ペプチドの一方向膜挿入による一価陽イオン選択性ポアの形成2010

    • Author(s)
      岩本真幸、老木成稔
    • Organizer
      日本生物物理学会第48回年会
    • Place of Presentation
      仙台市
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] カリウムチャネルと揮発性麻酔薬の分子作用-脂質平面膜法による実験系の確率をめざして-2010

    • Author(s)
      松木悠佳, 岩本真幸, 三田建一郎, 老木成稔, 重見研司
    • Organizer
      日本麻酔科学会東海・北陸支部第8回学術集会
    • Place of Presentation
      福井市
    • Year and Date
      2010-09-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] イオンチャンネル蛋白質(KcsA)の構造・機能1分子測定2010

    • Author(s)
      老木成稔
    • Organizer
      名古屋大学大学院医学系研究科
    • Place of Presentation
      名古屋市
    • Year and Date
      2010-07-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] KcsAチャネル蛋白質の1分子測定による構造ゆらぎと機能ゆらぎの相関2010

    • Author(s)
      老木成稔
    • Organizer
      新学術領域研究「揺らぎと生体機能」平成22年度合同班会議
    • Place of Presentation
      加賀市
    • Year and Date
      2010-06-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] 海綿由来細胞毒性ペプチドポリセオナミドBのイオンチャネル活性および細胞膜挿入機構2010

    • Author(s)
      岩本真幸, 清水啓史, 村松郁延, 松永茂樹, 老木成稔
    • Organizer
      第87回日本生理学会大会
    • Place of Presentation
      盛岡市
    • Year and Date
      2010-05-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 不活性化しない変異体であるE71A変異体を用いたKcsAカリウムチャネルのpH依存性2010

    • Author(s)
      松木悠佳, 岩本真幸, 清水啓史,老木成稔
    • Organizer
      第87回日本生理学会大会
    • Place of Presentation
      盛岡市
    • Year and Date
      2010-05-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] Structural and Functional Fluctuations of the KcsA Potassium Channel2009

    • Author(s)
      Oiki, S, H.Shimizu, M.Iwamoto, T.Konno
    • Organizer
      The 3^<rd> International Symposium : Molecular Science of Fluctuations toward Biological Functions
    • Place of Presentation
      名古屋
    • Year and Date
      2009-12-21
    • Related Report
      2009 Annual Research Report
  • [Presentation] Structural and Functional Fluctuations of the KcsA Potassium Channel.2009

    • Author(s)
      Oiki, S.
    • Organizer
      The 3^<rd> International Symposium : Molecular Science of Fluctuations toward Biological Functions.
    • Place of Presentation
      Nagoya
    • Year and Date
      2009-12-20
    • Related Report
      2010 Final Research Report
  • [Presentation] チャネルゲーティングの1分子構造変化ダイナミクス2009

    • Author(s)
      老木成稔
    • Organizer
      中部日本生理学会
    • Place of Presentation
      金沢
    • Year and Date
      2009-12-07
    • Related Report
      2009 Annual Research Report
  • [Presentation] Single-molecular measurements for structural and functional fluctuations of KcsA potassium channel.2009

    • Author(s)
      Oiki, S.
    • Organizer
      第47回生物物理学会年会シンポジウム「生体分子の揺らぎをはかる」
    • Place of Presentation
      徳島
    • Year and Date
      2009-10-30
    • Related Report
      2010 Final Research Report
  • [Presentation] Single-molecular measurements for structural and functional fluctuations of KcsA potassium channel2009

    • Author(s)
      老木成稔
    • Organizer
      第47回生物物理学会年会シンポジウム「生体分子の揺らぎをはかる」
    • Place of Presentation
      徳島
    • Year and Date
      2009-10-30
    • Related Report
      2009 Annual Research Report
  • [Presentation] KcsA カリウムチャネル構造機能相関の1分子解析2009

    • Author(s)
      老木成稔
    • Organizer
      第82日本生化学会大会. シンポジウム
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] チャネル蛋白質の1分子測定でみえる構造と機能 : イオン透過とゲーティング2009

    • Author(s)
      老木成稔
    • Organizer
      生物物理若手の会第49回夏の学校
    • Place of Presentation
      千歳
    • Year and Date
      2009-08-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] Real time monitoring of structural changes in K-channel gating.2009

    • Author(s)
      Oiki, S.
    • Organizer
      IUPS PSJ Symposium.
    • Place of Presentation
      Kyoto
    • Year and Date
      2009-07-29
    • Related Report
      2010 Final Research Report
  • [Presentation] Real time monitoring of structural changes in K-channel gating2009

    • Author(s)
      老木成稔
    • Organizer
      IUPS PSJ
    • Place of Presentation
      京都
    • Year and Date
      2009-07-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] Dynamics of KcsA Potassium Channel During pH-dependent Gating2009

    • Author(s)
      Oiki, S, H.Shimizu, M.Iwamoto, T.Konno
    • Organizer
      International Symposium on "Reaction Dynamics of Many-Body Chemical Systems"
    • Place of Presentation
      京都
    • Year and Date
      2009-06-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] Reaction Dynamics of Many-Body Chemical Systems2009

    • Author(s)
      Oiki, S.
    • Organizer
      International Symposium
    • Place of Presentation
      Kyoto
    • Related Report
      2010 Final Research Report
  • [Presentation] 一分子レベルでのチャネル構造変化のリアルタイム解析2008

    • Author(s)
      老木成稔
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-12
    • Related Report
      2008 Annual Research Report
  • [Presentation] KcsAカリウムチャネル開閉に伴うねじれ構図変化の1分子動画計測2008

    • Author(s)
      清水啓史, 岩本真幸, 今野卓二瓶亜三子, 佐々木裕二, 老木成稔
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2008-12-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] Global Twisting Motion of Single Molecular KcsA Potassium Channel Upon Gating.2008

    • Author(s)
      H. Shimizu, M. Iwanoto, T. Konnno, A. Nihei, Y. C. Sasaki, S. Oiki
    • Organizer
      国際シンポジウム New Approaches to Complexity of Protein Dynamics by Single Molecule Measurements : Experiments and Theories.
    • Place of Presentation
      Osaka
    • Year and Date
      2008-12-08
    • Related Report
      2008 Annual Research Report
  • [Presentation] 一分子構造変化の可視化によって明らかになったKcsAカリウムチャネルの開閉ねじれ運動2008

    • Author(s)
      清水啓史, 岩本真幸, 今野卓, 二瓶亜三子, 佐々木裕二, 老木成稔
    • Organizer
      第46回日本生物物理学会
    • Place of Presentation
      博多
    • Year and Date
      2008-12-05
    • Related Report
      2008 Annual Research Report
  • [Presentation] 流動電位測定によるKcsAカリウムチャネルのイオン透過機構の検討2008

    • Author(s)
      岩本真幸, 清水啓史, 老木成稔
    • Organizer
      第46回日本生物物理学会
    • Place of Presentation
      博多
    • Year and Date
      2008-12-05
    • Related Report
      2008 Annual Research Report
  • [Presentation] 電位依存性プロトンチャネルのプロトン透過の温度依存性2008

    • Author(s)
      久野みゆき, 安藤博之, 森畑宏一, 酒井啓, 森啓之, 清水啓史, 岩本真幸, 老木成稔
    • Organizer
      第46回日本生物物理学会
    • Place of Presentation
      博多
    • Year and Date
      2008-12-05
    • Related Report
      2008 Annual Research Report
  • [Presentation] ゲーティングに伴うカリウムチャネル構造変化の1分子解析2008

    • Author(s)
      老木成稔
    • Organizer
      第46回日本生物物理学会年会
    • Place of Presentation
      博多
    • Year and Date
      2008-12-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] Kチャネルのゲーティング構造変化について2008

    • Author(s)
      老木成稔, 清水啓史, 岩本真幸, 今野卓, 佐々木裕二
    • Organizer
      生理学研究所研究会
    • Place of Presentation
      岡崎
    • Year and Date
      2008-09-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] New Approaches to Complexity of Protein Dynamics by Single Molecule Measurements : Experiments and Theories.2008

    • Author(s)
      Shimizu, H., M.Iwamoto, T.Konno, A.Nihei, Y.C.Sasaki, S.Oiki
    • Organizer
      Global Twisting Motion of Single Molecular KcsA Potassium Channel upon Gating.
    • Place of Presentation
      Osaka
    • Related Report
      2010 Final Research Report
  • [Book] Modern Patch Clamp Techniques(The Planar Lipid Bilayer Method for the Study of Channel Molecules.)(Ed.Springer-Verlag)2011

    • Author(s)
      Oiki, S.
    • Publisher
      Springer-Verlag(in press.)
    • Related Report
      2010 Final Research Report
  • [Book] チャネル研究のための脂質平面膜法.「新パッチクランプ実験技術法」岡田泰伸編2011

    • Author(s)
      老木成稔
    • Publisher
      吉岡書店
    • Related Report
      2010 Annual Research Report
  • [Book] 序論 : イオンチャネル「新パッチクランプ実験技術法」岡田泰伸編2011

    • Author(s)
      久木田文夫、老木成稔
    • Publisher
      吉岡書店
    • Related Report
      2010 Annual Research Report
  • [Book] The Planar Lipid Bilayer Method for the Study of Channel Molecules. Modern Patch Clamp Techniques Y.Okada, Ed.2011

    • Author(s)
      Oiki, S.
    • Publisher
      Springer-Verlag(In press)
    • Related Report
      2010 Annual Research Report
  • [Book] Prologue : The Ion Channel. Modern Patch Clamp Techniques Y.Okada, Ed.2011

    • Author(s)
      Kukita, F, S.Oiki
    • Publisher
      Springer-Verlag
    • Related Report
      2010 Annual Research Report
  • [Book] Cell Signaling Reactions : Single-Molecular Kinetic Analysis(Single-Channel Structure-Function Dynamics : The Gating of Potassium Channels.)(Eds.Y.Sako, M.Ueda)2010

    • Author(s)
      Oiki, S.
    • Publisher
      Springer
    • Related Report
      2010 Final Research Report
  • [Book] Single-Channel Structure-Function Dynamics : The Gating of Potas sium Channels. Cell Signaling Reactions : Single-Molecular Kineti c Analysis. Eds. Y.Sako and M.Ueda2010

    • Author(s)
      Oiki, S.
    • Publisher
      Springer
    • Related Report
      2010 Annual Research Report
  • [Book] "Cell Signaling Reactions : Single-Molecular Kinetic Analysis"(Eds. Y. Sako and M. Ueda)(Springer)2010

    • Author(s)
      S.Oiki
    • Publisher
      Single-Channel Structure-Function Dynamics : The Gating of Potassium Channels(印刷中)
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://wwwl.fukui-med.ac.jp/home/ufms/file/kenkyu/welcome.html

    • Related Report
      2008 Annual Research Report
  • [Patent(Industrial Property Rights)] シスートランス液流方式によるイオンチャネル解析方法と、その方法に使用するイオンチャネル解析装置2010

    • Inventor(s)
      老木成稔・山内紀宏・坪田圭介
    • Industrial Property Rights Holder
      老木成稔・山内紀宏・坪田圭介
    • Acquisition Date
      2010-05-21
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 変異型HERGチャネル発現細胞およびその用途2005

    • Inventor(s)
      柳承希・明貝俊彦・今井友美・老木成稔
    • Industrial Property Rights Holder
      柳承希・明貝俊彦・今井友美・老木成稔
    • Industrial Property Number
      2005-269153
    • Filing Date
      2005-09-15
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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