The analysis of basic chromosomal functions using human artificial chromosomes (HACs) and the development of novel HACs.
| Project/Area Number |
20247019
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Molecular biology
|
|---|
| Research Institution | Kazusa DNA Research Institute |
|---|
Principal Investigator |
MASUMOTO Hiroshi Kazusa DNA Research Institute, ヒトゲノム研究部, 室長 (70229384)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
NAKANO Megumi (財団法人)かずさDNA研究所, 研究員 (50542825)
OHZEKI Jun-Ichirou (財団法人)かずさDNA研究所, 研究員 (30514088)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥44,070,000 (Direct Cost: ¥33,900,000、Indirect Cost: ¥10,170,000)
Fiscal Year 2010: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2009: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
Fiscal Year 2008: ¥22,620,000 (Direct Cost: ¥17,400,000、Indirect Cost: ¥5,220,000)
|
|---|
| Keywords | 遺伝学 / 染色体 / 分配 / セントロメア / 人工染色体 / クロマチン / エピジェネティックス / ヘテロクロマチン |
|---|
| Research Abstract |
We have developed a novel human artificial chromosome (HAC) system to investigate the epigenetic state of chromatin by applying a fusion-protein tethering system. We found that altering the HAC centromere chromatin to a more heterochromatic with the tTS transcription silencer or histone methyl transferase (Suv39h) caused mis-segregation of the HAC by losing kinetochore structure. Otherwise, histone acetyl transferase (HAT) fusions enhanced hyper-assembly of kinetocore components, indicating that a dynamic counterbalance is essential for mammalian kinetochore assembly.
|
Report
(4 results)
Research Products
(31 results)
-
-
-
[Journal Article] Human Artificial Chromosome with a Conditional Centromere for Gene Delivery and Gene Expression2010
Author(s)
Iida Y., Kim JH., Kazuki Y., Hoshiya H., Takiguchi M., Hayashi M., Erliandri I., Lee HS., Samoshkin A., Masumoto H., Earnshaw WC., Kouprina N., Larionov V., Oshimura M.
-
Journal Title
DNA Res. 17
Pages: 293-301
Related Report
Peer Reviewed
-
-
-
[Journal Article] Human gamma-satellite DNA maintains open chromatin structure and protects a transgene from epigenetic silencing.2009
Author(s)
Kim JH., Ebersole T., Kouprina N., Noskov V.N., Ohzeki J., Masumoto H., Mravinac B., Sullivan B.A., Pavlicek A., Dovat S., Pack S.D., Kwon YW., Flanagan P.T., Loukinov D., Lobanenkov V., Larionov V.
-
Journal Title
Genome Res. 19
Pages: 533-544
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
