GENETIC ANALYSIS OF AXIS FORMATION IN VERTEBRATES USING MEDAKA

• Project/Area Number: 20247030
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: The University of Tokyo
• Principal Investigator: TAKEDA Hiroyuki 東京大学, 大学院・理学系研究科, 教授 (80179647)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥44,200,000 (Direct Cost: ¥34,000,000、Indirect Cost: ¥10,200,000); Fiscal Year 2011: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2010: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2009: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2008: ¥25,090,000 (Direct Cost: ¥19,300,000、Indirect Cost: ¥5,790,000)
• Keywords: 左右軸 / 背腹軸 / メダカ / 突然変異体 / 器官形成 / 繊毛 / zic / 体節

Research Abstract

The study has demonstrated the novel mechanisms of left-right axis formation and late dorsoventral patterning using medaka developmental mutants. First, medaka left-right mutants have identified the novel cytoplasmic protein, Ktu required for axonemal dynein assembly together with molecular chaperons. Furthermore, the un-characterized membrane protein Pkd1l1 was found to serve as a flow sensor on motile cilia in the node region at an initial step of left-right determination. Finally, zic1/zic4 have crucial roles in dorsoventral patterning of the trunk region and in tail morphogenesis.

Research Products

• [Journal Article] The medaka zic1/zic4 mutant provides molecular insights into teleost caudal fin evolution. (2012)
  • Author(s): Yuuta Moriyama
  • Journal Title: Current Biology Volume: 21 Issue: 7 Pages: 601-607
  • DOI: 10.1016/j.cub.2012.01.063
  • Peer Reviewed
• [Journal Article] Pkd1l1 complexes withPkd2 on motile cilia and functions toestablish the left-right axis (2011)
  • Author(s): Kamura, K., Kobayashi, D., Uehara, Y., Koshida, S., et al
  • Journal Title: Development Volume: 138 Issue: 6 Pages: 1121-9
  • DOI: 10.1242/dev.058271
  • Peer Reviewed
• [Journal Article] Pkd1l1 complexes with Pkd2 on motile cilia and functions to establish the left-right axis. (2011)
  • Author(s): Kamura,K., Kobayashi, D., Uehara, U.,Koshida, S., Iijima, N., Kudo, A.,Yokoyama T., Takeda, H.
  • Journal Title: Development 138 Pages: 1121-1129
  • Peer Reviewed
• [Journal Article] The art of medaka genetics and genomics : what makesthem so unique (2010)
  • Author(s): Takeda, H., Shimada, A.
  • Journal Title: Annu Rev Genet Volume: 44 Pages: 217-41
  • Peer Reviewed
• [Journal Article] Single-cell analysis of somatotopic mapformation in the zebrafish lateral linesystem (2010)
  • Author(s): Sato, A., Koshida, S. & Takeda, H.
  • Journal Title: Dev Dyn Volume: 239 Issue: 7 Pages: 2058-65
  • DOI: 10.1002/dvdy.22324
  • Peer Reviewed
• [Journal Article] Emergence of traveling wave in thezebrafish segmentation clock (2010)
  • Author(s): Ishimatsu, K., Takamatsu, A., Takeda, H.
  • Journal Title: Development Volume: 137 Issue: 10 Pages: 1595-9
  • DOI: 10.1242/dev.046888
  • Peer Reviewed
• [Journal Article] FAK-mediated extracellularsignals are essential for interkineticnuclear migration and planar divisions inthe neuroepithelium (2010)
  • Author(s): Tsuda, S., Kitagawa, T., Takashima, S., et al
  • Journal Title: J Cell Sci Volume: 123 Issue: 3 Pages: 484-96
  • DOI: 10.1242/jcs.057851
  • Peer Reviewed
• [Journal Article] The art of medaka genetics and genomics : what makes them so unique? (2010)
  • Author(s): Takeda H., Shimada, A.
  • Journal Title: Ann Rev Genet 44 Pages: 217-241
  • Peer Reviewed
• [Journal Article] Single-cell analysis of somatotopic map formation in the zebrafish lateral line system. (2010)
  • Author(s): Sato, A., Koshida, S., Takeda, H.
  • Journal Title: Dev Dyn 239 Pages: 2058-2065
  • Peer Reviewed
• [Journal Article] Emergence of traveling wave in the zebrafish segmentation clock. (2010)
  • Author(s): Ishimatsu, K., Takamatsu, A., Takeda,H.
  • Journal Title: Development 137 Pages: 1595-1599
  • Peer Reviewed
• [Journal Article] FAK-mediated extracellular signals are essential for interkinetic nuclear migration and planar divisions in the neuroepithelium. (2010)
  • Author(s): Tsuda, S., Kitagawa, T., Takashima, S., Asakawa, S., Shimizu, N., Mitani, H., Shima, A., Tsutsumi, M., Hori, H., Naruse, K., Ishikawa, Y., Takeda, H.
  • Journal Title: J Cell Sci 123 Pages: 484-496
  • Peer Reviewed
• [Journal Article] FAK-mediated extracellular signals are essential for interkinetic nuclear migration and planar divisions in the neuroepithe-lium. (2010)
  • Author(s): Tsuda, S.
  • Journal Title: Journal of Cell Science Volume: 123 Pages: 484-496
  • Peer Reviewed
• [Journal Article] The art of medaka genetics and genomics : what makesthem so unique? (2010)
  • Author(s): Takeda, H.
  • Journal Title: Annal Review of Genetics Volume: 44 Pages: 217-241
  • Peer Reviewed
• [Journal Article] Emergence of traveling wave in the zebrafish segmentation clock. (2010)
  • Author(s): Ishimatsu, K
  • Journal Title: Development 137 Pages: 1595-1599
  • Peer Reviewed
• [Journal Article] FAK-mediated extracellular signals are essential for interkinetic nuclear migration and planar divisions in the neuroepithelium. (2010)
  • Author(s): Tsuda, S.
  • Journal Title: Journal of Cell Science 123 Pages: 484-496
  • Peer Reviewed
• [Journal Article] Characterization of teleost Mdga1 usinga gene-trap approach in medaka(Oryziaslatipes) (2009)
  • Author(s): Sano, S., Takashima, S., Niwa, H., et al
  • Journal Title: Genesis Volume: 47 Issue: 8 Pages: 505-13
  • DOI: 10.1002/dvg.20528
  • Peer Reviewed
• [Journal Article] Characterization of teleost Mdgal using a gene-trap approach in medaka (Oryzias latipes) (2009)
  • Author(s): Sano, S.
  • Journal Title: Genesis 47 Pages: 505-513
  • Peer Reviewed
• [Journal Article] Ktu/PF13 is required forcytoplasmic pre-assembly of axonemaldyneins (2008)
  • Author(s): Omran, H., Kobayashi, D., Olbrich, H., Tsukahara, T., Loges, N. T., Hagiwara, H., Zhang, Q., Leblond, G., Toole, E. O', Hara, C., Mizuno, H., Kawano, H., Fliegauf, M., Yagi, T., Koshida, S., Miyawaki, A., Zentgraf, H., Seithe, H., Reinhardt, R., Watanabe,
  • Journal Title: Nature Volume: 456 Issue: 7222 Pages: 611-6
  • DOI: 10.1038/nature07471
  • Peer Reviewed
• [Journal Article] Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins. (2008)
  • Author(s): Omran, H., Kobayashi, D., Olbrich, H.,Tsukahara, T., Loges, N. T., Hagiwara, H., Zhang, Q., Leblond, G., Toole, E.O', Hara, C., Mizuno, H., Kawano, H., Fliegauf, M., Yagi, T., Koshida, S., Miyawaki, A., Zentgraf, H., Seithe, H., Reinhardt, R., Watanabe, Y., Kamiya, R., Mitchell, D.R., Takeda, H.
  • Journal Title: Nature 456 Pages: 611-616
  • Peer Reviewed
• [Journal Article] Ktu/PF13 is required for cytoplasmic pre-assembly of axonemal dyneins (2008)
  • Author(s): Omran, H.
  • Journal Title: Nature 456 Pages: 611-616
  • Peer Reviewed
• [Presentation] Epigenetic regulation ofkey developmental genes. genome wideapproaches in medaka fish (2012)
  • Author(s): Takeda, H.
  • Organizer: The firstbilateral India-Japan DevelopmentalBiology Workshop
  • Place of Presentation: バンガロール
  • Year and Date: 2012-01-08
• [Presentation] 発生後期におけるzic1/zic4を介した背腹パターン決定機構 (2011)
  • Author(s): 島田敦子
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-22
• [Presentation] 発生後期におけるzic1/zic4を介した背腹パターン決定機構 (2011)
  • Author(s): 島田敦子
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 国立京都国際会館(京都府)(招待講演)
  • Year and Date: 2011-09-22
• [Presentation] zic1/4は真骨魚類における正型尾形成に必須である (2011)
  • Author(s): 守山裕大
  • Organizer: 日本動物学会第82回旭川大会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-21
• [Presentation] zic1/4は真骨魚類における正型尾形成に必須である (2011)
  • Author(s): 守山裕大
  • Organizer: 日本動物学会第82回旭川大会
  • Place of Presentation: 大雪クリスタルホール(北海道)
  • Year and Date: 2011-09-21
• [Presentation] Mesodermal origin of scales and fins of the medaka-Insight into origin and evolution of mineralized skeleton in vertebrates (2011)
  • Author(s): 武田洋幸
  • Organizer: JSDB-GFE Joint Meeting ofDevelopmental Bioligy
  • Place of Presentation: ドレスデン
  • Year and Date: 2011-03-24
• [Presentation] Mesodermal origin of scales and fins of the medaka-Insight into origin and evolution of mineralized skeleton in vertebrates (2011)
  • Author(s): Takeda, H.
  • Organizer: JSDB-GFE Joint Meeting of Developmental Bioligy
  • Place of Presentation: Dresden,Germany
• [Presentation] Analyses of the cytoplasmicprotein Ktu function in axonemaldyne information during motile ciliogenesis (2010)
  • Author(s): 松尾萌
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Pkd1l1/Pkd2 as nodalflow sensor to regulate left-right asymmetry (2010)
  • Author(s): 加村啓一郎
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Embryonic origin of osteoblasts in scales and fins of medaka fish (2010)
  • Author(s): 島田敦子
  • Organizer: 2010 SDB-JSDB JointMeeting
  • Place of Presentation: アルバカーキ
  • Year and Date: 2010-08-05
• [Presentation] zic1 and zic4 expression in the somite regulates dorsalization of the fish trunk structures (2010)
  • Author(s): 河西通
  • Organizer: 2010 SDB-JSDB Joint Meeting
  • Place of Presentation: アルバカーキ
  • Year and Date: 2010-08-05
• [Presentation] The zic genes as masterregulators in dorsoventral patterning of the fish trunkstructures (2010)
  • Author(s): 守山裕大
  • Organizer: 2^<nd> Joint Meeting of the SFBD and JSDB 2010
  • Place of Presentation: パリ
  • Year and Date: 2010-05-27
• [Presentation] Single-Cell Analysis of Somatotopic Map Formation in the Zebrafish Lateral Line System (2010)
  • Author(s): 佐藤朗
  • Organizer: 2^<nd> Joint Meeting of the SFBD and JSDB 2010
  • Place of Presentation: パリ
  • Year and Date: 2010-05-27
• [Presentation] The zic genes asmaster regulators in dorsoventral patterning of the fish trunkstructures (2010)
  • Author(s): 武田洋幸
  • Organizer: CDBシンポジウム
  • Place of Presentation: 神戸
  • Year and Date: 2010-03-24
• [Presentation] The zic genes as master regulators in dorsoventral patterning of the fish trunk structures (2010)
  • Author(s): Takeda, H.
  • Organizer: CDB symposium
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2010-03-24
• [Presentation] The zic genes as master regulators in dorsoventral patterning of the fish trunk structures (2010)
  • Author(s): Moriyama, Y., Nakamura, R., Kawanishi, T., Shimada, A., Takeda, H.
  • Organizer: 2nd Joint Meeting of the SFBD and JSDB
  • Place of Presentation: Paris, France
• [Presentation] Single-Cell Analysis of Somatotopic Map Formation in the Zebrafish Lateral Line System (2010)
  • Author(s): Sato, A., Koshida, S., Takeda, H.
  • Organizer: 2nd Joint Meeting of the SFBD and JSDB
  • Place of Presentation: Paris, France
• [Presentation] Embryonic origin of osteoblasts in scales and fins of medaka fish (2010)
  • Author(s): Shimada, A., Kawanishi, T., Inohaya, K., Takeda, H.
  • Organizer: 2010 SDB-JSDB Joint Meeting
  • Place of Presentation: Albuquerque, U.S.A.
• [Presentation] zic1 and zic4 expression in the somite regulates dorsalization of the fish trunk structures (2010)
  • Author(s): Kawanishi, T., Moriyama, Y., Nakamura, R., Shimada, A., Takeda, H.
  • Organizer: 2010 SDB-JSDB Joint Meeting
  • Place of Presentation: Albuquerque, U.S.A.
• [Presentation] The zic genes as master regulators in dorsoventral patterning of the fish trunk structures (2010)
  • Author(s): Moriyama, Y.
  • Organizer: 2^<nd> Joint Meeting of the SFBD and JSDB 2010
  • Place of Presentation: パリ(フランス)
• [Presentation] Analyses of the cytoplasmic protein Ktu function in axonemal dynein formation during motile ciliogenesis (2010)
  • Author(s): Matsuo, M.
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸市神戸ポートランド
• [Presentation] Pkd111/Pkd2 as nodal flow sensor to regulate left-right asymmetry (2010)
  • Author(s): Kamura, K.
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸市神戸ポートランド
• [Presentation] Left-right mutantmedaka identifies a candidate sensor of leftward flow (2009)
  • Author(s): 加村啓一郎
  • Organizer: 16^<th> ISDB 2009
  • Place of Presentation: スコットランド
  • Year and Date: 2009-09-06
• [Presentation] Left-right mutant medaka identifies a candidate sensor of leftward flow (2009)
  • Author(s): Kamura, K.
  • Organizer: 16^<th> ISDB 2009
  • Place of Presentation: Scotland, UK
• [Presentation] 左右軸から繊毛形成まで-メダカ変異体を用いた遺伝学的解析 (2008)
  • Author(s): 武田洋幸
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] Analysis of the somite-specific Enhancer of the zic Genes in Medaka : Toward Understanding of the Evolution of the Body Shape in Teleosts (2008)
  • Author(s): 守山裕大
  • Organizer: The 16^<th> CDB Meeting
  • Place of Presentation: 神戸
  • Year and Date: 2008-09-28
• [Presentation] Analysis of the somite-specific Enhancer of the zic Genes in Medaka : Toward Understanding of the Evolution of the Body Shape in Teleosts (2008)
  • Author(s): Moriyama, Y.
  • Organizer: The 16^<th> CDB Meeting
  • Place of Presentation: Kobe, Japan
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/hassei/
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/hassei/
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/hassei/
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/hassei/takeda/TOP%20main/TOP.html