Gene regulatory network involved in the formation of central nervous system in ascidian embryos

• Project/Area Number: 20247031
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Kyoto University
• Principal Investigator: SATOH Noriyuki Kyoto University, マリンゲノミックスユニット, 代表研究者 (30025481)
• Co-Investigator(Kenkyū-buntansha): TAKAMURA Katsumi 福山大学, 生命工学部, 准教授 (30206883) ; SASAKURA Yasunori 筑波大学, 大学院・生命環境科学研究科, 講師 (10400649)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥42,640,000 (Direct Cost: ¥32,800,000、Indirect Cost: ¥9,840,000); Fiscal Year 2010: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000); Fiscal Year 2009: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000); Fiscal Year 2008: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
• Keywords: 脊索動物 / ホヤ / 中枢神経系 / 発生と分化 / 遺伝子発現 / 遺伝子ネットワーク / トランスジェニック系 / マイクロアレイ / 感覚砲(脳) / 神経細胞 / 上衣細胞 / トランスジェニック系統 / 神経ペプチド / 脳の領域化 / 脊椎動物 / 細胞分化 / GFP / 特異的遺伝子

Research Abstract

Despite containing only approximately 350 cells, the central nervous system (CNS) of Ciona intestinalis larvae has an architecture that is similar to the vertebrate CNS. To identify genes expressed in the CNS, we isolated CNS cells using transgenic embryos that showed robust Kaede expression in the larval CNS. With the aid of microarray, we identified 565 genes that are preferentially expressed in the CNS. In addition, tracing cells with specific gene expression and functional analyses of the genes resulted in several discoveries of the gene regulatory network involved in the formation of the Ciona larval CNS.

Research Products

• [Journal Article] Ependymal cells of chordate larvae arestem-like cells that form the adult nervoussystem. (2011)
  • Author(s): Horie, T., Shinki, R., Ogura, Y., Kusakabe, T. G., Satoh, N., Sasakura, Y.
  • Journal Title: Nature 469 Pages: 525-528
• [Journal Article] Expression of neuropeptide- and hormone-encoding genes in the Ciona intestinalis larval brain. (2011)
  • Author(s): Hamada, M., Shimozono, N., Ohta, N., Satou, Y., Horie, T., Kawada, T., Satake, H., Sasakura, Y., Satoh, N.
  • Journal Title: Dev Biol 352 Pages: 202-214
• [Journal Article] Coordination of mitosis and morphogenesis: role of a prolonged G2 phase during chordate neurulation. (2011)
  • Author(s): Ogura, Y., Sakaue-Sawano, A., Nakagawa, M., Satoh, N., Miyawaki, A., Sasakura, Y.
  • Journal Title: Development 138 Pages: 577-587
• [Journal Article] Expression of neuropeptide-and hormone-encoding genes in the Ciona intestinalis larval brain. (2011)
  • Author(s): Hamada, M., et al.
  • Journal Title: Developmental Biology Volume: 352 Pages: 202-214
  • Peer Reviewed
• [Journal Article] Ependymal cells of chordate larvae are stem-like cells that form the adult nervous system. (2011)
  • Author(s): Horie, T., et al.
  • Journal Title: Nature Volume: 469 Pages: 525-528
  • Peer Reviewed
• [Journal Article] Efficient transposition of a single Minos transposon copy in the genome of the ascidian Ciona intestinalis with a transgenic line expressing transposase in eggs. (2010)
  • Author(s): Hozumi, A., Kawai, N., Yoshida, R., Ogura, Y., Ohta, N., Satake, H., Satoh, N., Sasakura, Y.
  • Journal Title: Dev Dyn 239 Pages: 1076-1088
• [Journal Article] Network structure of projections extending from peripheral neurons in the tunic of ascidian larva. (2010)
  • Author(s): Terakubo, H.Q., Nakajima, Y., Sasakura, Y., Horie, T., Konno, A., Takahashi, H., Inaba, K., Hotta, K., Oka, K.
  • Journal Title: Dev Dyn 239 Pages: 2278-2287
• [Journal Article] Differential regional expression of genes in the developing brain of Ciona intestinalis embryos. (2010)
  • Author(s): Shimozono, N., Ohta, N., Satoh, N., Hamada, M.
  • Journal Title: Zoolog Sci 27 Pages: 103-109
• [Journal Article] Neural map of the larval central nervous system in the ascidian Ciona intestinalis. (2010)
  • Author(s): Takamura, K., Minamida, N., Okabe S.
  • Journal Title: Zoolog Sci 27 Pages: 191-203
  • NAID: 40017358570
• [Journal Article] Differential regional expression of genes in the developing brain of Ciona intestinalis embryos (2010)
  • Author(s): Shimozono, N., et al.
  • Journal Title: Zoological Science Volume: 27 Pages: 103-109
  • Peer Reviewed
• [Journal Article] Differential regional expression of genes in the developing brain of Ciona intestinalis embryos. (2010)
  • Author(s): Shimozono,N.
  • Journal Title: Zoological Science 27 Pages: 103-109
  • Peer Reviewed
• [Journal Article] Neural map of the larval central nervous system in the ascidian Ciona intestinalis. (2010)
  • Author(s): Takamura,K.
  • Journal Title: Zoological Science 27 Pages: 191-203
  • NAID: 40017358570
  • Peer Reviewed
• [Journal Article] Delineating metamorphic pathways in the ascidian Ciona intestinalis. (2009)
  • Author(s): Nakayama,A.
  • Journal Title: Developmental Biology 326 Pages: 357-367
  • Peer Reviewed
• [Presentation] Beyond the Conklin's idea (2010)
  • Author(s): Satoh, N.
  • Organizer: Society of Developmental Biologists, USA
  • Place of Presentation: Albuquerque, New Mexico, USA
  • Year and Date: 2010-08-08
• [Presentation] Ependymal cells of chordate larvae are stem-like cells that form the adult nervous system. (2010)
  • Author(s): Horie, T., Shinki, R., Ogura, Y., Kusakabe, T.G., Satoh, N., Sasakura, Y.
  • Organizer: The 33^<rd> Annual Meeting of Molecular Biology of Japan
  • Place of Presentation: Kobe, Japan.
• [Presentation] Beyond the Conklin's Idea. (2010)
  • Author(s): Satoh, N.
  • Organizer: Society of Developmental Biologists Annual Meeting
  • Place of Presentation: New Mexico, USA
• [Presentation] Ptf1, a bHLH transcription facto, defines the dopaminergic neuronal cell fate in ascidian larva. (2009)
  • Author(s): Horie,T.
  • Organizer: 5^<th> International Tunicate Meeting
  • Place of Presentation: Naha, Okinawa
  • Year and Date: 2009-06-24
• [Presentation] Identification of the regulatory regions and transcriptionalfactors involved in the specific expression of the cholinergic genes in Ciona intestinalis (2009)
  • Author(s): Takamura, K.
  • Organizer: 5^<th> International Tunicate Meeting
  • Place of Presentation: Okinawa
• [Remarks] ホームページ等
  • URL: http://www.irp.oist.jp/satoh/
• [Remarks]
  • URL: http://www.shimoda.ra/index.html
• [Remarks]
  • URL: http://www.irp.oist.jp/satoh/
• [Remarks]
  • URL: http://www.irp.oist.jp/satoh
• [Remarks]
  • URL: http://ghost.zool.kyoto-u.ac.jp