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Gene regulatory network involved in the formation of central nervous system in ascidian embryos

Research Project

Project/Area Number 20247031
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionKyoto University

Principal Investigator

SATOH Noriyuki  Kyoto University, マリンゲノミックスユニット, 代表研究者 (30025481)

Co-Investigator(Kenkyū-buntansha) TAKAMURA Katsumi  福山大学, 生命工学部, 准教授 (30206883)
SASAKURA Yasunori  筑波大学, 大学院・生命環境科学研究科, 講師 (10400649)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥42,640,000 (Direct Cost: ¥32,800,000、Indirect Cost: ¥9,840,000)
Fiscal Year 2010: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2009: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Fiscal Year 2008: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Keywords脊索動物 / ホヤ / 中枢神経系 / 発生と分化 / 遺伝子発現 / 遺伝子ネットワーク / トランスジェニック系 / マイクロアレイ / 感覚砲(脳) / 神経細胞 / 上衣細胞 / トランスジェニック系統 / 神経ペプチド / 脳の領域化 / 脊椎動物 / 細胞分化 / GFP / 特異的遺伝子
Research Abstract

Despite containing only approximately 350 cells, the central nervous system (CNS) of Ciona intestinalis larvae has an architecture that is similar to the vertebrate CNS. To identify genes expressed in the CNS, we isolated CNS cells using transgenic embryos that showed robust Kaede expression in the larval CNS. With the aid of microarray, we identified 565 genes that are preferentially expressed in the CNS. In addition, tracing cells with specific gene expression and functional analyses of the genes resulted in several discoveries of the gene regulatory network involved in the formation of the Ciona larval CNS.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (23 results)

All 2011 2010 2009 Other

All Journal Article (13 results) (of which Peer Reviewed: 6 results) Presentation (5 results) Remarks (5 results)

  • [Journal Article] Ependymal cells of chordate larvae arestem-like cells that form the adult nervoussystem.2011

    • Author(s)
      Horie, T., Shinki, R., Ogura, Y., Kusakabe, T. G., Satoh, N., Sasakura, Y.
    • Journal Title

      Nature 469

      Pages: 525-528

    • Related Report
      2010 Final Research Report
  • [Journal Article] Expression of neuropeptide- and hormone-encoding genes in the Ciona intestinalis larval brain.2011

    • Author(s)
      Hamada, M., Shimozono, N., Ohta, N., Satou, Y., Horie, T., Kawada, T., Satake, H., Sasakura, Y., Satoh, N.
    • Journal Title

      Dev Biol 352

      Pages: 202-214

    • Related Report
      2010 Final Research Report
  • [Journal Article] Coordination of mitosis and morphogenesis: role of a prolonged G2 phase during chordate neurulation.2011

    • Author(s)
      Ogura, Y., Sakaue-Sawano, A., Nakagawa, M., Satoh, N., Miyawaki, A., Sasakura, Y.
    • Journal Title

      Development 138

      Pages: 577-587

    • Related Report
      2010 Final Research Report
  • [Journal Article] Expression of neuropeptide-and hormone-encoding genes in the Ciona intestinalis larval brain.2011

    • Author(s)
      Hamada, M., et al.
    • Journal Title

      Developmental Biology

      Volume: 352 Pages: 202-214

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ependymal cells of chordate larvae are stem-like cells that form the adult nervous system.2011

    • Author(s)
      Horie, T., et al.
    • Journal Title

      Nature

      Volume: 469 Pages: 525-528

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient transposition of a single Minos transposon copy in the genome of the ascidian Ciona intestinalis with a transgenic line expressing transposase in eggs.2010

    • Author(s)
      Hozumi, A., Kawai, N., Yoshida, R., Ogura, Y., Ohta, N., Satake, H., Satoh, N., Sasakura, Y.
    • Journal Title

      Dev Dyn 239

      Pages: 1076-1088

    • Related Report
      2010 Final Research Report
  • [Journal Article] Network structure of projections extending from peripheral neurons in the tunic of ascidian larva.2010

    • Author(s)
      Terakubo, H.Q., Nakajima, Y., Sasakura, Y., Horie, T., Konno, A., Takahashi, H., Inaba, K., Hotta, K., Oka, K.
    • Journal Title

      Dev Dyn 239

      Pages: 2278-2287

    • Related Report
      2010 Final Research Report
  • [Journal Article] Differential regional expression of genes in the developing brain of Ciona intestinalis embryos.2010

    • Author(s)
      Shimozono, N., Ohta, N., Satoh, N., Hamada, M.
    • Journal Title

      Zoolog Sci 27

      Pages: 103-109

    • Related Report
      2010 Final Research Report
  • [Journal Article] Neural map of the larval central nervous system in the ascidian Ciona intestinalis.2010

    • Author(s)
      Takamura, K., Minamida, N., Okabe S.
    • Journal Title

      Zoolog Sci 27

      Pages: 191-203

    • NAID

      40017358570

    • Related Report
      2010 Final Research Report
  • [Journal Article] Differential regional expression of genes in the developing brain of Ciona intestinalis embryos2010

    • Author(s)
      Shimozono, N., et al.
    • Journal Title

      Zoological Science

      Volume: 27 Pages: 103-109

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential regional expression of genes in the developing brain of Ciona intestinalis embryos.2010

    • Author(s)
      Shimozono,N.
    • Journal Title

      Zoological Science 27

      Pages: 103-109

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neural map of the larval central nervous system in the ascidian Ciona intestinalis.2010

    • Author(s)
      Takamura,K.
    • Journal Title

      Zoological Science 27

      Pages: 191-203

    • NAID

      40017358570

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Delineating metamorphic pathways in the ascidian Ciona intestinalis.2009

    • Author(s)
      Nakayama,A.
    • Journal Title

      Developmental Biology 326

      Pages: 357-367

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Beyond the Conklin's idea2010

    • Author(s)
      Satoh, N.
    • Organizer
      Society of Developmental Biologists, USA
    • Place of Presentation
      Albuquerque, New Mexico, USA
    • Year and Date
      2010-08-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Ependymal cells of chordate larvae are stem-like cells that form the adult nervous system.2010

    • Author(s)
      Horie, T., Shinki, R., Ogura, Y., Kusakabe, T.G., Satoh, N., Sasakura, Y.
    • Organizer
      The 33^<rd> Annual Meeting of Molecular Biology of Japan
    • Place of Presentation
      Kobe, Japan.
    • Related Report
      2010 Final Research Report
  • [Presentation] Beyond the Conklin's Idea.2010

    • Author(s)
      Satoh, N.
    • Organizer
      Society of Developmental Biologists Annual Meeting
    • Place of Presentation
      New Mexico, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] Ptf1, a bHLH transcription facto, defines the dopaminergic neuronal cell fate in ascidian larva.2009

    • Author(s)
      Horie,T.
    • Organizer
      5^<th> International Tunicate Meeting
    • Place of Presentation
      Naha, Okinawa
    • Year and Date
      2009-06-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] Identification of the regulatory regions and transcriptionalfactors involved in the specific expression of the cholinergic genes in Ciona intestinalis2009

    • Author(s)
      Takamura, K.
    • Organizer
      5^<th> International Tunicate Meeting
    • Place of Presentation
      Okinawa
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.irp.oist.jp/satoh/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.shimoda.ra/index.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.irp.oist.jp/satoh/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.irp.oist.jp/satoh

    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://ghost.zool.kyoto-u.ac.jp

    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-10-28  

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