Studies on the mechanisms of leaf formation from the viewpoint of shoot meristem function

• Project/Area Number: 20248001
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: The University of Tokyo
• Principal Investigator: NAGATO Yasuo The University of Tokyo, 大学院・農学生命科学研究科, 教授 (10143413)
• Co-Investigator(Kenkyū-buntansha): ITOH JunーIchi 東京大学, 大学院・農学生命科学研究科, 准教授 (30345186)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥47,060,000 (Direct Cost: ¥36,200,000、Indirect Cost: ¥10,860,000); Fiscal Year 2010: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000); Fiscal Year 2009: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000); Fiscal Year 2008: ¥21,710,000 (Direct Cost: ¥16,700,000、Indirect Cost: ¥5,010,000)
• Keywords: シュートメリステム / イネ / 突然変異 / 葉パターン / 葉間期 / SAM / ADL遺伝子 / PLA遺伝子 / NDL1遺伝子 / WAF1遺伝子 / 育種学 / 発生・分化 / 発現制御 / 遺伝学

Research Abstract

This research project has been carried out to reveal the mechanisms of leaf formation in relation to shoot meristem. We identified WAF1 gene that was associated with shoot meriatem maintenance and leaf morphogenesis. WAF1 functions in the regulation of small RNAa. As for temporal regulation of leaf primordium formation, we newly identified PLA3 gene. Together with the results of PLA1 and PLA2, it is indicated that signals from preexisting leaf primordia play important role on the formation of new leaf primordium. We also identified genes that regulate adaxial-abaxial, central-lateral and basal-apical patterns of leaves.

Research Products

• [Journal Article] Ovule is a lateral organ finally differentiated from the terminating floral meristem in rice. (2011)
  • Author(s): Yamaki S, Nagato Y., Kurata N., Nonomura K-I.
  • Journal Title: Dev.Biol. 351 Pages: 208-216
  • Peer Reviewed
• [Journal Article] Ovule is a lateral organ finally differentiated from the terminating floral meristem in rice (2011)
  • Author(s): Yamaki, S., Nagato, Y., Kurata, N., Nonomura, KI.
  • Journal Title: Develop, ental Biology Volume: 351 Pages: 208-216
  • Peer Reviewed
• [Journal Article] Ovule is a lateral organ finally differentiated from the terminating floral meristem in rice (2011)
  • Author(s): Yamaki, S., Nagato, Y., Kurata, N., Nonomura, K-I.
  • Journal Title: Develop,ental Biology Volume: 3 Pages: 208-216
  • Peer Reviewed
• [Journal Article] WAVY LEAF 1, an ortholog of Arabidopsis HEN1, regulates shoot development by maintaining microRNA and trans-acting siRNA accumulation in rice. (2010)
  • Author(s): Abe M., Yoshikawa T., Nosaka M., Sakakibara H., Sato Y., Nagato Y., Itoh J.-I.
  • Journal Title: Plant Physiol. 154 Pages: 1335-1346
  • Peer Reviewed
• [Journal Article] Abe, M., Yoshikawa, T., Nosaka, M., Sakakibara, H., Sato, Y., Nagato, Y.and Rob, J.I.(2010) WAVYLEAF 1, an ortholog of Arabidopsis HEM, regulates shoot development by maintaining microRNA and trans-acting siRNA accumulation in rice (2010)
  • Author(s): Abe, M., Yoshikawa, T., Nosaka, M., Sakakibara, H., Sato, Y., Nagato, Y., Itoh, J.I.
  • Journal Title: Plant Physiology Volume: 154 Pages: 1335-1346
  • Peer Reviewed
• [Journal Article] Mappring and characterization of the rice gene, CLUB-SHAPED EMBRYO3, CLE3. (2010)
  • Author(s): Imamura, K.Nagato, Y., Matsuoka, M., Kitano, H., Sazuka, T
  • Journal Title: Rice Genetics Newsletter Volume: 25 Pages: 127-129
  • Peer Reviewed
• [Journal Article] Studies of sodium bypass flow in lateral rootless mutant Lrt1, Lrt2 and crown rootless mutant Crll of rice (Oryza sativa L.) (2010)
  • Author(s): Faiyue, B., Vijayalakshim, C., Nawaz, S., Nagato, Y., Taketa, S., Ichii, M., Al-Azzawi, Flowers, T.
  • Journal Title: Plant Cell Environment Volume: 33:00:00 Pages: 687-701
  • Peer Reviewed
• [Journal Article] Abe, M., Yoshikawa, T., Nosaka, M., Sakakibara, H., Sato, Y., Nagato, Y. and Itoh, J.-I. (2010) WAVY LEAF 1, an ortholog of Arabidopsis HEN1, regulates shoot development by maintaining microRNA and trans-acting siRNA accumulation in rice (2010)
  • Author(s): Abe, M.,, Yoshikawa, T., Nosaka, M., Sakakibara, H., Sato, Y., Nagato, Y., Itoh, J.-I.
  • Journal Title: Plant Physiology Volume: 154 Pages: 1335-1346
  • Peer Reviewed
• [Journal Article] Studies of sodium bypass flow in lateral rootless mutant Lrt1, Lrt2 and crown rootless mutant Crl1 of rice (Oryza sativa L.) (2010)
  • Author(s): Faiyue, B., Vijayalakshim, C., Nawaz, S., Nagato, Y., Taketa, S., Ichii, M., Al-Azzawi, Flowers, T.
  • Journal Title: Plant Cell Environment Volume: 33:00:00 Pages: 687-701
  • Peer Reviewed
• [Journal Article] MOSAIC FLORAL ORGANS1, an AGL6-Like MADS-Box gene, controls floral organ identity and meristem fate in rice. (2009)
  • Author(s): Ohmor S., Kimizu M., Sugita M., Miyao A., Hirochika H., Uchida E., Nagato Y., Yohida, H.
  • Journal Title: Plant Cell 21 Pages: 3008-3025
  • Peer Reviewed
• [Journal Article] A rice tryptophan deficient dwarf 1 mutant, tdd1, contains a reduced level of Indole acetic acid and develops abnormal flowers and organless embryos. (2009)
  • Author(s): Sazuka T., Kamiya N., Nushimura T., Ashikari M., Ohmae K., Imamura K., Nagato Y., Koshiba T., Kitano H., Matsuoka M.
  • Journal Title: Plant J. 60 Pages: 227-241
  • Peer Reviewed
• [Journal Article] The ADAXIALIZED LEAF1 gene functions in leaf and embryonic pattern formation lin rice. (2009)
  • Author(s): Hibara K., Obara M., E.Hayashida, Ishimaru T., Abe M., Satoh H., Itoh J.-I., Nagato Y.
  • Journal Title: Dev.Biol. 334 Pages: 345-354
  • Peer Reviewed
• [Journal Article] PLASTOCHRON3/GOLOATH encodes a glutamate carboxypeptidase required for proper development in rice. (2009)
  • Author(s): Kawakatsu T., Taramino G., Itoh J.-I., Allen J., Sato Y., Hng S.-K., Nagasawa N., Kojima M., Kusaba M., Sakakibara H., Sakai H., Nagato Y.
  • Journal Title: Plant J. 58 Pages: 1028-1040
  • Peer Reviewed
• [Journal Article] OPEN BEAK is a negative regulator of class 1 knox genes and a positive regulator of class B floral homeotic gene in rice. (2009)
  • Author(s): Horigome A., Nagasawa N., Ikeda K., Ito M., Itoh J.-I., Nagato Y.
  • Journal Title: Plant J. 58
  • Peer Reviewed
• [Journal Article] PLASTOCHRON3/GOLOATH encodes a glutamate carboxypeptidase required for proper development in rice (2009)
  • Author(s): Kawakatsu, T.
  • Journal Title: Plant Journal 58 Pages: 1028-1040
  • Peer Reviewed
• [Journal Article] OPEN BEAK is a negative regulator of class 1 knox genes and a positive regulator of class B floral homeotic gene in ric (2009)
  • Author(s): Horigome, A.
  • Journal Title: Plant Journal 58 Pages: 724-736
  • Peer Reviewed
• [Journal Article] Genetically engineered rice that contains higher amounts of nicotianamine to enhance the antihypertensive effect (2009)
  • Author(s): Usuda, K.
  • Journal Title: Plant Biotechnology Journal 7 Pages: 87-95
  • Peer Reviewed
• [Journal Article] SHOOT ORGANIZATION2 gene coordinates leaf domain development along the central-marginal axis in rice. (2008)
  • Author(s): Itoh J.-I., Sato Y., Nagato Y.
  • Journal Title: Plant Cell Physiol. 49 Pages: 1226-1236
  • Peer Reviewed
• [Journal Article] Developmental role and auxin responsibility of class III HD-ZIP gene family members in rice. (2008)
  • Author(s): Itoh J.-I., Hibara K., Sato Y., Nagato Y.
  • Journal Title: Plant Physiol. 147 Pages: 1960-1975
  • Peer Reviewed
• [Journal Article] The rice FLATTENED SHOOT MERISTEM, encoding chromatin assembly factor-1 p150 subunit, is required for meristem maintenance by regulating cell-cycle period. (2008)
  • Author(s): Abe M., Kuroshita H., Umeda M., Itoh J.-I., Nagato, Y.
  • Journal Title: Dev.Biol. 319 Pages: 384-393
  • Peer Reviewed
• [Journal Article] SHOOT ORGANIZATION2 gene coordinates leaf domain development along the central-marginal axis in rice (2008)
  • Author(s): Itoh, J.-I.
  • Journal Title: Plant & Cell Physiology 49 Pages: 1226-1236
  • Peer Reviewed
• [Journal Article] Developmental role and auxin responsibility of class III HD-ZIP gene family members in rice (2008)
  • Author(s): Itoh, J.-I.
  • Journal Title: Plant Physiology 147 Pages: 1960-1975
  • Peer Reviewed
• [Journal Article] FLATTENED SHOOT MERISTEM, encoding chromatin assembly factor-1 p150 subunit, is required for meristem maintenance by regulating cell-cycle period (2008)
  • Author(s): Abe, M.
  • Journal Title: Developmental Biology 319 Pages: 384-393
  • Peer Reviewed
• [Journal Article] MNU-induced mutant pools and high performance TILLING enable finding of any gene mutation in rice (2008)
  • Author(s): Suzuki, T.
  • Journal Title: Mol.Genet.Genomics 279 Pages: 213-223
  • Peer Reviewed
• [Presentation] イネのvegetative phase changeに関わる変異体の解析 (2011)
  • Author(s): 田中伸裕・伊藤博紀・千徳直樹・榊原均・井澤毅・伊藤純一・長戸康郎
  • Organizer: 日本育種学会
  • Place of Presentation: 横浜市立大学
  • Year and Date: 2011-03-29
• [Presentation] リボソームタンパク遺伝子がイネの初期生育に及ぼす影響 (2011)
  • Author(s): 細木渉・伊藤純一・長戸康郎
  • Organizer: 日本育種学会
  • Place of Presentation: 横浜市立大学
  • Year and Date: 2011-03-29
• [Presentation] イネvegetative phase changeに関わる変異体の解析 (2011)
  • Author(s): 田中伸裕・伊藤博紀・千徳直樹・榊原均・井澤毅・伊藤純一・長戸康郎
  • Organizer: 日本育種学会
  • Place of Presentation: 横浜市立大学(神奈川県)
  • Year and Date: 2011-03-29
• [Presentation] リボゾームタンパク遺伝子がイネの初期生育に及ぼす影響 (2011)
  • Author(s): 細木渉・伊藤純一・長戸康郎
  • Organizer: 日本育種学会
  • Place of Presentation: 横浜市立大学(神奈川県)
  • Year and Date: 2011-03-29
• [Presentation] plastochron変異体のホルモン応答性と原因遺伝子の過剰発現 (2010)
  • Author(s): 三村真生・伊藤純一・長戸康郎
  • Organizer: 日本育種学会第118回講演会
  • Place of Presentation: 秋田県立大学(秋田県)
  • Year and Date: 2010-09-25
• [Presentation] イネSLENDER LEAF1遺伝子の解析 (2010)
  • Author(s): 吉川貴徳・伊藤純一・長戸康郎
  • Organizer: 日本育種学会第118回講演会
  • Place of Presentation: 秋田県立大学(秋田県)
  • Year and Date: 2010-09-25
• [Presentation] plastochron変異体のホルモン応答性と原因遺伝子の過剰発現 (2010)
  • Author(s): 三村真生・伊藤純一・長戸康郎
  • Organizer: 日本育種学会
  • Place of Presentation: 秋田県立大学
  • Year and Date: 2010-09-25
• [Presentation] イネSLENDER LEAF1遺伝子の解析 (2010)
  • Author(s): 吉川貴徳・伊藤純一・長戸康郎
  • Organizer: 日本育種学会
  • Place of Presentation: 秋田県立大学
  • Year and Date: 2010-09-25
• [Presentation] イネの多様な発生突然変異と農業形質 (2010)
  • Author(s): 長戸康郎
  • Organizer: 第49回ガンマーフィールドシンポジウム
  • Place of Presentation: 水戸市
  • Year and Date: 2010-07-14
• [Presentation] イネの多様な発生突然変異と農業形質 (2010)
  • Author(s): 長戸康郎
  • Organizer: 第49回ガンマーフィールドシンポジウム
  • Place of Presentation: 水戸市(茨城県)
  • Year and Date: 2010-07-14
• [Presentation] イネ細葉変異体slender leafの解析 (2010)
  • Author(s): 吉川貴徳・小野絵里・伊藤淳一・長戸康郎
  • Organizer: 日本育種学会第117回講演会
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2010-03-27
• [Presentation] NARUTOMAKI gene affects the leaf rolling and viability in rice. (2010)
  • Author(s): Yew C., J.-I.Itoh, Y.Nagato
  • Organizer: 117th Annual Meeting of Japanese Society of Breeding
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2010-03-27
• [Presentation] イネのメバロン酸経路の遺伝子SEGMENTED EMBRYOは胚の分割を抑制する (2010)
  • Author(s): 高階泰宗・伊藤純一・長戸康郎
  • Organizer: 日本育種学会第117回講演会
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2010-03-26
• [Presentation] イネのjaponica-indicaにおけるjuvenile-adult相転換時期の分化 (2010)
  • Author(s): 荒川理恵、吉川貴徳、伊藤純一・長戸康郎
  • Organizer: 日本育種学会第117回講演会
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2010-03-26
• [Presentation] 栄養成長初期に主桿が枯死するイネのmain culm lethal変異体の解析 (2010)
  • Author(s): 藤井宏樹、伊藤純一、長戸康郎
  • Organizer: 日本育種学会第117回講演会
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2010-03-26
• [Presentation] Identification and characterization of rice ABERRANT PANICLE ORGANIZATION 2 gene. (2010)
  • Author(s): Ikeda-Kawakatsu K. Sato H., Izawa T., Maekawa M., Nagato Y
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学(熊本県)
  • Year and Date: 2010-03-18
• [Presentation] 葉序を規定するイネDEC遺伝子の単離 (2009)
  • Author(s): 伊藤純一、榊原均、長戸康郎
  • Organizer: 日本育種学会第116回講演会
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2009-09-25
• [Presentation] イネの初期生育に異常を示すasy変異体の解析 (2009)
  • Author(s): 細木渉、伊藤純一、長戸康郎
  • Organizer: 日本育種学会第116回講演会
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2009-09-25
• [Presentation] plastochron変異体のホルモン応答性と原因遺伝子の過剰発現 (2009)
  • Author(s): 三村真生・伊藤純一・長戸康郎
  • Organizer: 日本育種学会
  • Place of Presentation: 秋田県立大学(秋田県)
  • Year and Date: 2009-09-25
• [Presentation] イネSLENDER LEAF1遺伝子の解析 (2009)
  • Author(s): 吉川貴徳・伊藤純一・長戸康郎
  • Organizer: 日本育種学会
  • Place of Presentation: 秋田県立大学(秋田県)
  • Year and Date: 2009-09-25
• [Presentation] イネの胚の器官分化パターンを維持できないmsp1変異体の解析 (2009)
  • Author(s): 田附博、伊藤純一、草場信、北野英己、長戸康郎
  • Organizer: 日本育種学会第115回講演会
  • Place of Presentation: エポカルつくば(茨城県)
  • Year and Date: 2009-03-28
• [Presentation] イネのPETER PAN SYNDROME遺伝子はjuvenile-adult相転換と光形態形成を制御する (2009)
  • Author(s): 田中伸裕、伊藤純一、千徳直樹、長戸康郎
  • Organizer: 日本育種学会第115回講演会
  • Place of Presentation: エポカルつくば(茨城県)
  • Year and Date: 2009-03-28
• [Presentation] イネspw1-cls変異体の閉花受粉性に対する温度の影響 (2009)
  • Author(s): 大森伸之介、佐藤光、長戸康郎、吉田均
  • Organizer: 日本育種学会第115回講演会
  • Place of Presentation: エポカルつくば(茨城県)
  • Year and Date: 2009-03-28
• [Presentation] イネの胚の器官分化パターンを維持できないmsp1変異体の解析 (2009)
  • Author(s): 田附博
  • Organizer: 日本育種学会第115回講演会
  • Place of Presentation: つくば
  • Year and Date: 2009-03-28
• [Presentation] イネのPETER PAN SYNDROME遺伝子は juvenile-adult 相転換と光形態形成を制御するす (2009)
  • Author(s): 田中伸裕
  • Organizer: 日本育種学会第115回講演会
  • Place of Presentation: つくば
  • Year and Date: 2009-03-28
• [Presentation] イネADAXIALIZED LEAF遺伝子は葉や胚のパターン形成に作用する (2008)
  • Author(s): 桧原健一郎、小原真理、林田恵美、阿部匡、伊藤純一、長戸康郎
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2008-12-11
• [Presentation] イネ胚パターン形成変異体are2の同定と解析 (2008)
  • Author(s): 田附博、伊藤百代、伊藤純一、長戸康郎
  • Organizer: 日本育種学会第114回講演会
  • Place of Presentation: 滋賀県立大学(滋賀県)
  • Year and Date: 2008-10-11
• [Presentation] 細胞間接着を負に制御するLEAF ADHESION遺伝子の解析 (2008)
  • Author(s): 松井三可子、佐藤亜依、伊藤純一、長戸康郎
  • Organizer: 日本育種学会第114回講演会
  • Place of Presentation: 滋賀県立大学(滋賀県)
  • Year and Date: 2008-10-11
• [Presentation] イネ胚パターン形成変異体are2の同定と解析 (2008)
  • Author(s): 田附博
  • Organizer: 日本育種学会第114回講演会
  • Place of Presentation: 彦根
  • Year and Date: 2008-10-11
• [Presentation] 細胞間接着を負に制御するLEAF ADHESION遺伝子の解析 (2008)
  • Author(s): 松井三可子
  • Organizer: 日本育種学会第114回講演会
  • Place of Presentation: 彦根
  • Year and Date: 2008-10-11
• [Presentation] イネADAXIALIZED LEAF遺伝子は葉や胚のパターン形成に作用する (2008)
  • Author(s): 桧原健一郎
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会 合同大会
  • Place of Presentation: 神戸