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MOLECULAR BASIS OF S-RNASE-BASED GAMETOPHYTIC SELF-INCOMPATIBILITY IN PRUNUS IN THE ROSACEAE

Research Project

Project/Area Number 20248004
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Horticulture/Landscape architecture
Research InstitutionKyoto University

Principal Investigator

TAO Ryutaro  京都大学, 大学院・農学研究科, 准教授 (10211997)

Co-Investigator(Kenkyū-buntansha) YAMANE Hisayo  京都大学, 大学院・農学研究科, 講師 (80335306)
HABU Tsuyoshi  京都大学, 大学院・農学研究科, 助教 (60335304)
Project Period (FY) 2008 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥39,130,000 (Direct Cost: ¥30,100,000、Indirect Cost: ¥9,030,000)
Fiscal Year 2011: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2010: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2009: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2008: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000)
Keywordsアーモンド / アンズ,ウメ / オウトウ / 核果類 / スモモ / S-Rnase / SFB / アンズ / ウメ / S-RNase / 共通因子 / 自家和合性
Research Abstract

Prunus in the Rosaceae show the gametophytic self-incompatibility system, which hinders effective fruit production and breeding in Prunus. This study was started to elucidate the molecular basis of the gametophytic self-incompatibility in Prunus. We identified pollen expressed actin that binds to the pistil Sdeterminant, S-RNase. Furthermore, we identified a Skp1-like protein called SSK1 that binds to the pollen Sdeterminant, SFB. We have cloned Cul1 that forms the SCF complex with SSK1 and SFB. Based on the results obtained, we have developed the working hypothesis for the molecular recognition of the gametophytic self-incompatibility in Prunus.

Report

(6 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report   Self-evaluation Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (34 results)

All 2012 2011 2010 2009 2008 Other

All Journal Article (21 results) (of which Peer Reviewed: 21 results) Presentation (11 results) Remarks (2 results)

  • [Journal Article] Identification of a Skp1-like protein interacting with SFB, the pollen Sdeterminant of the gametophytic self-incompatibility in Prunus2012

    • Author(s)
      Matumoto, D., H. Yamane, K. Abe, and R. Tao
    • Journal Title

      Plant Physiology

      Volume: 159 Issue: 3 Pages: 12562-1262

    • DOI

      10.1104/pp.112.197343

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of pollen-expressed actin as a candidate protein interacting with S-RNase in Prunus aviumL.2012

    • Author(s)
      Matsumoto, D. and R. Tao.
    • Journal Title

      J. Japan. Soc. Hort. Sci.

      Volume: 81 Pages: 41-47

    • NAID

      130004510740

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of Pollen-expressed Actin as a Candidate Protein Interacting with S-RNase in Prunus avium L.2012

    • Author(s)
      Matsumoto, D., R.Tao
    • Journal Title

      Journal of the Japanese Society for Horticultural Science

      Volume: 81 Issue: 1 Pages: 41-47

    • DOI

      10.2503/jjshs1.81.41

    • NAID

      130004510740

    • ISSN
      1882-3351, 1882-336X
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of quantitative trait loci associated with self-compatibility in a Prunus species2011

    • Author(s)
      Martf, A.F., W.Howad, R.Tao, J.M.Alonso, P.Araus, R.Socias i Company
    • Journal Title

      Tree Genetics and Genomes

      Volume: 7 Issue: 3 Pages: 629-639

    • DOI

      10.1007/s11295-010-0362-2

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular and genetic analyses of four non-functional Shaplotype variant derived from a common ancestral S haplotype identified in sour cherry (Prunus cerasusL.).2010

    • Author(s)
      Tsukamoto, T., N.R. Hauck, R. Tao, N. Jiang, and A.F. Iezzoni.
    • Journal Title

      Genetics

      Volume: 184 Pages: 411-427

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular and genetic analyses of four non-functional S haplotype variant derived from a common ancestral S haplotype identified in sour cherry (Prunus cerasus L.).2010

    • Author(s)
      Tsukamoto, T., N.R. Hauck, R. Tao, N. Jiang, A.F. Iezzoni
    • Journal Title

      Genetics 184

      Pages: 411-427

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] The S-RNase-based gametophytic self-incompatibility system in Prunus exhibits distinct genetic and molecular features2010

    • Author(s)
      Tao, R., A.F.Iezzoni
    • Journal Title

      Scientia Horticulturae

      Volume: 124 Pages: 137-157

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular and genetic analyses of four nonfunctional S haplotype variants derived from a common ancestral S haplotype identified in sour cherry (Prunus cerasus L.)2010

    • Author(s)
      Tsukamoto, T., N.R.Hauck, R.Tao, N.Jiang, A.F.Iezzoni
    • Journal Title

      Genetics

      Volume: 184 Pages: 411-427

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Self-compatibility in 'Cristobalina' sweet cherry is not associated with duplications or modified transcription levels of S-locus genes2010

    • Author(s)
      Wunsch, A., R.Tao, J.I.Hormaza
    • Journal Title

      Plant Cell Report

      Volume: 29 Pages: 715-721

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular basis of self-(in)compatibility and current status of S-genotyping in rosaceous fruit trees2009

    • Author(s)
      Yamane, H. and R. Tao.
    • Journal Title

      J. Japan. Hort. Sci

      Volume: 78 Pages: 137-157

    • NAID

      130004510656

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A modifier locus affecting the expression of the S-RNase gene could be the cause of breakdown of self-incompatibility in almond. Sex2009

    • Author(s)
      Fernandez i Marti, A., T. Hanada, J.M. Alonso, H. Yamane, R. Tao, and R. Socias i Company.
    • Journal Title

      Plant Reprod.

      Volume: 22 Pages: 179-186

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cloning and characterization of a self-compatible Sfhaplotype in almond (Prunus dulcis(mill.) D. A. Webb. syn. P. amygladusBatsch) to ressolve previous confusion in its Sf-RNasesequence.2009

    • Author(s)
      Hanada, T., K. Fukuta., H. Yamane, T. Esumi, and R. Tao.
    • Journal Title

      HortScience

      Volume: 44 Pages: 609-613

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular basis of self-(in)compatibility and current status of S-genotyping in rosaceous fruit trees.2009

    • Author(s)
      Yamane, H., R. Tao
    • Journal Title

      J.Japan.Hort.Sci. 78

      Pages: 137-157

    • NAID

      130004510656

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] A modifier locus affecting the expression of the S-RNase gene could be the cause of breakdown of self-incompatibility in almond.2009

    • Author(s)
      Fernandez i Marti, A., T. Hanada, J.M. Alonso, H. Yamane, R. Tao, R. Socias i Company
    • Journal Title

      Sex.Plant Reprod. 22

      Pages: 179-186

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Cloning and characterization of a self-compatible Sf haplotype in almond (Prunus dulcis (mill.) D.A.Webb.syn.P amygladus Batsch) to ressolve previous confusion in its Sf-RNase sequence.2009

    • Author(s)
      Hanada, T., K. Fukuta., H. Yamane, T. Esumi, R. Tao
    • Journal Title

      HortScience 44

      Pages: 609-613

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Molecular basis of self-(in) compatibility and current status of S-genotyping in rosaceous fruit trees2009

    • Author(s)
      Yamane, H., R.Tao
    • Journal Title

      Journal of the Japanese Society for Horticultural Science

      Volume: 78 Pages: 137-157

    • NAID

      130004510656

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A modifire locus affecting the expression of the S-RNase gene could be the cause of breakdown of self-incompatibility in almond2009

    • Author(s)
      Fernandez i Marti, A., T.Hanada, J.M.Alonso, H.Yamane, R.Tao, R.Socias i Company
    • Journal Title

      Sexual Plant Reproduction

      Volume: 22 Pages: 179-186

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cloning and characterization of a self-compatible Sf haplotype in almond [Prunus dulcis (mill.) D.A.Webb.syn.P.amygdalus Batsch to resolve previous donfusion in its Sf-RNase sequence2009

    • Author(s)
      Hanada, T., K.Fukuta, H.Yamane, T.Esumi, R.Tao
    • Journal Title

      HortScience

      Volume: 44 Pages: 609-613

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of SLFL1, a pollen-expressed F-box gene located in the Prunus Slocus. Sex2008

    • Author(s)
      Matsumoto, D., H. Yamane, and R. Tao
    • Journal Title

      Plant Reprod

      Volume: 21 Pages: 113-121

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of SLFL1, a pollen-expressed F-box gene located in the Prunus S locus.2008

    • Author(s)
      Matsumoto, D., H. Yamane, R. Tao
    • Journal Title

      Sex.Plant Reprod. 21

      Pages: 113-121

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Characterization of SLFLl, a pollen-expressed F-box gene located in the Prunus S locus2008

    • Author(s)
      Matumoto, D., H. Yamane, R. Tao
    • Journal Title

      Sezual Plant Reproduction 21

      Pages: 113-121

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] バラ科サクラ属における自家不和合性花粉側因子SFBとSCF複合体構成要素間の相互作用解析2012

    • Author(s)
      松本大生・田尾龍太郎
    • Organizer
      平成24年度園芸学会春季大会
    • Place of Presentation
      大阪府立大学中百舌鳥キャンパス
    • Related Report
      2011 Final Research Report
  • [Presentation] 不和合および和合受粉したウメ雌ずいのRNA-seq解析2012

    • Author(s)
      羽生剛・尾畑智哉・田尾龍太郎
    • Organizer
      平成24年度園芸学会春季大会
    • Place of Presentation
      大阪府立大学中百舌鳥キャンパス
    • Related Report
      2011 Final Research Report
  • [Presentation] カンカオウトウにおけるS-RNase結合花粉タンパク質としてのアクチンホモログの単離2011

    • Author(s)
      松本大生・田尾龍太郎
    • Organizer
      平成23年度園芸学会秋季大会
    • Place of Presentation
      岡山大学津島キャンパス
    • Related Report
      2011 Final Research Report
  • [Presentation] 育種・栽培化に伴うモモのS locusの進化2011

    • Author(s)
      赤木剛士・花田俊男・八重垣英明・山口正巳・田尾龍太郎
    • Organizer
      平成23年度園芸学会秋季大会
    • Place of Presentation
      岡山大学津島キャンパス
    • Related Report
      2011 Final Research Report
  • [Presentation] アーモンドの自家和合性に関わるSfハプロタイプの解析2010

    • Author(s)
      田尾龍太郎・Fernandez, A.・赤木剛士・花田俊男・Alonso, J.・Socias i Company, R.
    • Organizer
      平成22年度園芸学会秋季大会
    • Place of Presentation
      大分大学旦野原キャンパス
    • Year and Date
      2010-09-20
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] アーモンドの自家和合性に関わるSf ハプロタイプの解析2010

    • Author(s)
      田尾龍太郎・Fernandez, A. ・赤木剛士・花田俊男・Alonso, J.・Socias i Company, R
    • Organizer
      平成22年度園芸学会秋季大会
    • Place of Presentation
      大分大学旦野原キャンパス
    • Related Report
      2011 Final Research Report
  • [Presentation] モモとモモ近縁種におけるSハプロタイプの共通性2009

    • Author(s)
      花田俊男・木部隆則・江角智也・山根久代・笹辺幸男・八重垣秀明・山口正己・Dandekar, A.M., Gradziel, T.M.・田尾龍太郎.
    • Organizer
      平成21年度園芸学会秋季大会
    • Place of Presentation
      秋田大学手形キャンパス
    • Related Report
      2011 Final Research Report 2010 Self-evaluation Report
  • [Presentation] Sハプロタイプの解析により示唆されたモモの自家和合化機構2009

    • Author(s)
      花田俊男・木部隆則・渡晶子・江角智也・山根久代・笹辺幸男・八重垣秀明・山口正己・Dandekar・A.M., Gradziel・T.M., Wunschi, A. ・田尾龍太郎
    • Organizer
      平成21年度園芸学会秋季大会
    • Place of Presentation
      秋田大学手形キャンパス
    • Related Report
      2011 Final Research Report 2010 Self-evaluation Report
  • [Presentation] モモにおける自家和合性Sハプロタイプのクローニングと塩基配列解析2009

    • Author(s)
      花田俊男・渡晶子・笹辺幸男・八重垣秀明・山口正己・田尾龍太郎
    • Organizer
      平成21年度園芸学会春季大会
    • Place of Presentation
      明治大学駿河台キャンパス
    • Related Report
      2011 Final Research Report 2010 Self-evaluation Report
  • [Presentation] サクラ属果樹における花粉側因子SFBと相互作用するSkp1の同定2008

    • Author(s)
      松本大生・山根久代・田尾龍太郎
    • Organizer
      平成20年度園芸学会秋季大会
    • Place of Presentation
      三重大学共通教育棟・生物資源学部棟
    • Year and Date
      2008-09-28
    • Related Report
      2010 Self-evaluation Report
  • [Presentation] サクラ属果樹における花粉側因子SFBと相互作用するSkp1の同定2008

    • Author(s)
      松本大生・山根久代・田尾龍太郎.
    • Organizer
      平成20年度園芸学会秋季大会
    • Place of Presentation
      三重大学生物資源学部
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.pomology.kais.kyoto-u.ac.jp/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.pomology.kais.kyoto-u.ac.jp/

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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