Cloning of avirulence genes in Pyricularia spp. using super SAGE and comparative analysis of their instability

• Project/Area Number: 20248005
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Kobe University
• Principal Investigator: TOSA Yukio 神戸大学, 大学院・農学研究科, 教授 (20172158)
• Co-Investigator(Kenkyū-buntansha): TERAUCHI Ryohei 岩手生物工学研究センター, 主席研究員 (50236981) ; KUSABA Motoaki 佐賀大学, 農学部, 准教授 (90304881)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥46,540,000 (Direct Cost: ¥35,800,000、Indirect Cost: ¥10,740,000); Fiscal Year 2011: ¥10,530,000 (Direct Cost: ¥8,100,000、Indirect Cost: ¥2,430,000); Fiscal Year 2010: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000); Fiscal Year 2009: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000); Fiscal Year 2008: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
• Keywords: いもち病 / 非病原力遺伝子 / Magnaporthe oryzae / Pyricularia oryzae / avirulence gene / Manarthe orzae / Pricularia orzae / Avirulence gene / Rice / Blast / blast

Research Abstract

We found that AVR-Pita, AVR-Pia, AVR-Pii, AVR-Pik, avirulence genes of the rice blast fungus, are highly variable in its genome location, occurring in chromosomes 1 through 7 and supernumerary chromosomes. They were flanked by retrotransposons, which presumably contributed to their "multiple translocation" across the genome during the course of evolution in response to corresponding resistance genes. We also found an avirulence gene that has not undergone multiple translocation. We suggest that the stability of avirulence genes is predictable on the basis of the their chromosomal locations

Research Products

• [Journal Article] Instability of subtelomeric regions during meiosis in Magnaporthe oryzae (2011)
  • Author(s): Chuma, I., Hotta, Y., and Tosa, Y.
  • Journal Title: J. Gen. Plant Pathol Volume: 77 Pages: 317-325
  • NAID: 10030332319
• [Journal Article] Multiple translocation of the AVR-Pita effector gene among chromosomes of the rice blast fungus Magnaporthe oryzae and related species (2011)
  • Author(s): Chuma, I., Isobe, C., Hotta, Y., Ibaragi, K., Futamata, N., Kusaba, M., Yoshida, K., Terauchi, R., Fujita, Y., Nakayashiki, H., Valent, B., and Tosa, Y.
  • Journal Title: PLoS Pathogens
• [Journal Article] Multiple translocation of the AVR-Pita effector gene among chromosomes of the rice blast fungus Magnaporthe oryzae and related species (2011)
  • Author(s): Chuma, et al
  • Journal Title: PloS Pathogens Volume: e1002147(web) Issue: 7 Pages: 1020147-1020147
  • DOI: 10.1371/journal.ppat.1002147
  • Peer Reviewed
• [Journal Article] Instability of subtelomeric regions during meiosis in Magnaporthe oryzae (2011)
  • Author(s): Chuma, et al
  • Journal Title: J.Gen.Plant Pathol. Volume: 77 Issue: 6 Pages: 317-325
  • DOI: 10.1007/s10327-011-0338-6
  • Peer Reviewed
• [Journal Article] PWT1, an avirulence gene of Magnaporthe oryzae tightly linked to the rDNA locus, is recognized by two staple crops (2010)
  • Author(s): Chuma, I., Zhan, S. W., Asano, S., Nga, N. T. T., Vy, T. T. P., Shirai, M., Ibaragi, K., and Tosa, Y.
  • Journal Title: common wheat and barley. Phytopathology Volume: 100 Pages: 436-443
• [Journal Article] PWT1, an avirulence gene of Magnaporthe oryzae tightly linked to the rDNA locus, is recognized by two staple crops, common wheat and barley. (2010)
  • Author(s): Chuma, I., Zhan, S.W., Asano, S., Nga, N.T.T., Vy, T.T.P., Shirai, M., Ibaragi, K., Tosa, Y.
  • Journal Title: Phytopathology lOO Pages: 436-443
  • Peer Reviewed
• [Journal Article] PWT1, an avirulence gene of Magnaporthe oryzae tightly linked to the rDNA locus, is recognized by two staple crops, common wheat and barley (2010)
  • Author(s): Chuma, et al.
  • Journal Title: Phytopathology Volume: 100 Pages: 436-443
  • Peer Reviewed
• [Journal Article] Evolution of the Eleusine subgroup of Pyricularia oryzae inferred from rearrangement at the Pwll locus (2010)
  • Author(s): Tanaka, et al.
  • Journal Title: Mol.Plant-Microbe Interact. Volume: 23 Pages: 771-783
  • Peer Reviewed
• [Journal Article] PWT1, an avirulence gene of Magnaporthe oryzae tightly linked to the rDNA locus, is recognized by two staple crops, common wheat and barley (2010)
  • Author(s): Chuma, et al.
  • Journal Title: Phytopathology 100(In press)
  • Peer Reviewed
• [Journal Article] Evolution of the Eleusine subgroup of Pyricularia oryzae inferred from rearrangement at the Pwl1 locus (2010)
  • Author(s): Tanaka, et al.
  • Journal Title: Mol.Plant-Microbe Interact. 23(In press)
  • Peer Reviewed
• [Journal Article] Association genetics reveals three novel avirulence genes from the rice blast fungal pathogen Magnaporthe oryzae (2009)
  • Author(s): Yoshida, K., Saitoh, H., Fujisawa, S., Kanzaki, H., Matsumura, H., Yoshida, K., Tosa, Y., Chuma, I., Takano, Y., Win, J., Kamoun, S., and Terauchi, R.
  • Journal Title: Plant Cell Volume: 21 Pages: 1573-1591
• [Journal Article] Association genetics reveals three novel avirulence genes from the rice blast fungal pathogen Magnnporthe oryzae. (2009)
  • Author(s): Yoshida, K., Saitoh, H., Fujisawa, S., Kanzaki, H., Matsumura, H., Yoshida, K., Tosa, Y., Chuma, I., Takano, Y., Win, J., Kamoun, S., Terauchi, R.
  • Journal Title: Plant Cell 21 Pages: 1573-1591
  • Peer Reviewed
• [Journal Article] Association genetics reveals three novel avirulence genes from the rice blast fungal pathogen Magnaporthe oryzae. (2009)
  • Author(s): Yoshida, et al.
  • Journal Title: Plant Cell 21 Pages: 1573-1591
  • Peer Reviewed
• [Journal Article] An avirulence gene to rice cultivar K60 is located on the 1. 6-Mb chromosome in Magnaporthe oryzae isolate 84R-62B (2008)
  • Author(s): Kusaba, M., Luo, C. X., Hanamura, H., Misaka, M., Mochida, T., Fujita, Y., and Tosa, Y.
  • Journal Title: J. Gen. Plant Pathol Volume: 74 Pages: 250-253
• [Presentation] Roles of avirulence genes of Pyricularia species in defining host and nonhost specificity. (2011)
  • Author(s): Tosa, Y.
  • Organizer: Plant and Animal Genome XIX.
  • Place of Presentation: San Diego, California, U.S.A.
  • Year and Date: 2011-01-15
• [Presentation] Roles of avirulence genes of Pyricularia species in defining host and nonhost specificity (2011)
  • Author(s): Tosa, Y.
  • Organizer: Plant and Animal genome XIX
  • Place of Presentation: San Diego, CA(USA)(招待講演)
  • Year and Date: 2011-01-15
• [Presentation] Roles of avirulence genes o Pyricularia species in defining host and nonhost specificity (2011)
  • Author(s): Tosa, Y.
  • Organizer: Plant and Animal Genome XIX
  • Place of Presentation: San Diego, CA. USA
• [Presentation] Genetic analyses of host species specificity of Magnaparthe oryzae subgroups. (2010)
  • Author(s): Tosa, Y.
  • Organizer: 10th Japan-US Seminar.
  • Place of Presentation: Corvallis, Oregon, U.S.A.
  • Year and Date: 2010-01-26
• [Presentation] Genetic analyses of host species specificity of Magnaporthe oryzae subgroups (2010)
  • Author(s): Tosa, Y., Chuma, I.
  • Organizer: 10th Japan-US Seminar
  • Place of Presentation: Oregon State University(U.S.A.)
  • Year and Date: 2010-01-26
• [Presentation] Genetic analyses of host species specificity of Mangaporthe oryzae subgroups (2010)
  • Author(s): Tosa, Y. and Chuma I.
  • Organizer: 10^<th> Japan-US Seminar
  • Place of Presentation: Oregon, USA
• [Presentation] いもち病菌における非病原力遺伝子の比較解析とその進化過程に関する考察 (2009)
  • Author(s): 中馬いづみ, 他8名
  • Organizer: 平成21年度日本植物病理学会大会
  • Place of Presentation: 山形市
  • Year and Date: 2009-03-26
• [Presentation] エンバクいもち病菌のコムギに対する非病原力遺伝子PWT4へのクロモソームウオーキング (2009)
  • Author(s): 井上喜博, 他3名
  • Organizer: 平成21年度日本植物病理学会大会
  • Place of Presentation: 山形市
  • Year and Date: 2009-03-26
• [Presentation] いもち病菌集団における非病原力遺伝子AVR-Piiの分布 (2009)
  • Author(s): 古田純一, 他5名
  • Organizer: 平成21年度日本植物病理学会大会
  • Place of Presentation: 山形市
  • Year and Date: 2009-03-26
• [Book] Searching for effectors of Magnaporthe oryzae : a multi-faceted genomics approach (2009)
  • Author(s): Terauchi, R., Win, J., Kamoun, S., Matsumura, H., Saitoh, H., Kanzaki, H., Yoshida, K., Shenton, M., Berberich, T., Fujisawa, S., Ito, A., Takano, Y., and Tosa, Y.
  • Publisher: Springer
• [Book] Biology of Molecular Plant-Microbe Interactions (2008)
  • Author(s): Terauchi, R., Win, J., Kamoun, S., Matsumura, H., Saitoh, H., Yoshida, K., Shenton, M., Berberich, T., Fujisawa, S., Ito, A., Takano, Y., and Tosa, Y.
  • Total Pages: 114