Molecular bases of electron transport component selectivity in aromatic ring-hydroxylating dioxygenase system
| Project/Area Number |
20248010
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NOJIRI Hideaki 東京大学, 生物産工学研究センター, 准教授 (90272468)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥48,750,000 (Direct Cost: ¥37,500,000、Indirect Cost: ¥11,250,000)
Fiscal Year 2010: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2009: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2008: ¥34,970,000 (Direct Cost: ¥26,900,000、Indirect Cost: ¥8,070,000)
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| Keywords | 芳香族化合物 / オキシゲナーゼ / 電子伝達 / タンパク質間相互作用 / 結晶構造 / 結合定数 / biodegradation / 細菌 / 芳香環水酸化オキシゲナーゼ / タンパク質問相互作用 |
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| Research Abstract |
Crystal structures of reduced form of ferredoxin component (Fd) and complex form of oxidized Fd and reduced oxygenase (Oxy) of carbazole 1,9a-dioxygeanse were solved. Cleavages of salt-bridge between Oxy R118 and Fd E43 and hydrogen-bonding between Oxy R210 and Fd E55 were detected along with the reduction of Oxy. Alanine replacement analyses revealed these bindings are cooperatively enhanced the binding between Oxy and Fd. Isothermal titration calorimetry analyses also revealed that the binding through hydrophobic interaction between the components were stabilized by surrounding salt-bridge and hydrogen-bonding.
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Report
(4 results)
Research Products
(39 results)
