• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Search for antitumor marine natural products through growth recovery assay in engineered yeasts

Research Project

Project/Area Number 20248022
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

MATSUNAGA Shigeki  東京大学, 大学院・農学生命科学研究科, 教授 (60183951)

Co-Investigator(Renkei-kenkyūsha) YOSHIDA Minoru  独立行政法人理化学研究所, 吉田化学遺伝学研究室, 主任研究員 (80191617)
Project Period (FY) 2008 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥38,220,000 (Direct Cost: ¥29,400,000、Indirect Cost: ¥8,820,000)
Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2008: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Keywords天然物化学 / 遺伝子導入酵母 / 抗腫瘍性 / 酵母 / 遺伝子導入 / 変異 / カイメン / 抗腫瘍 / 生育阻害 / 生育回復 / 抗腫瘍活性 / 活性物質 / 単離 / 構造決定 / 海綿
Research Abstract

Most important issue in the natural products discovery study is how to link the high-through-put in vitro assay results and cell-based in vivo activities. The growth recovery assay of the otherwise lethal gene-overexpressing mutant yeasts constructed by Dr. Yoshida is a method of choice for such purposes. From the extracts of marine invertebrates we isolated two truncated thiamine derivatives from the marine sponge Theonella swinhoei and three steroid glycosides from an unidentified marine sponge.

Report

(7 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report   Self-evaluation Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (1 results)

All 2013

All Presentation (1 results)

  • [Presentation] 遺伝子組換え酵母を用いた小臥蛇島産カイメンからの新規ステロイド配糖体の単離および構造決定2013

    • Author(s)
      鈴木真志ほか6名
    • Organizer
      平成25年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学
    • Year and Date
      2013-03-27
    • Related Report
      2012 Annual Research Report 2012 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi