| Project/Area Number |
20248029
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Okayama University |
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Principal Investigator |
KUNIEDA Tetsuo 岡山大学, 大学院・自然科学研究科, 教授 (80178011)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJI Takehito 岡山大学, 大学院・自然科学研究科, 准教授 (90314682)
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| Co-Investigator(Renkei-kenkyūsha) |
UCHIDA Kazuyuki 東京大学, 大学院・農学生命科学研究科, 准教授 (10223554)
MORITOMO Yasuo 東海大学, 農学部, 教授 (70148972)
YAMATO Osamu 鹿児島大学, 農学部, 教授 (80261337)
WATANABE Daisaku 北里大学, 獣医学部, 准教授
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥47,710,000 (Direct Cost: ¥36,700,000、Indirect Cost: ¥11,010,000)
Fiscal Year 2010: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Fiscal Year 2009: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
Fiscal Year 2008: ¥21,970,000 (Direct Cost: ¥16,900,000、Indirect Cost: ¥5,070,000)
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| Keywords | 育種 / 和牛 / 遺伝性疾患 / 遺伝子診断 / 黒毛和種 / ポジショナルクローニング / 連鎖解析 / 突然変異 / ウシ / 家畜 / 筋形成 / マイクロサテライトマーカー |
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| Research Abstract |
We have attempted to identify the gene responsible for hereditary disorders of Japanese Black cattle and develop the genetic diagnosis systems to detect the mutations. Consequently, we have revealed that a nonsense mutation of the GFRA1 gene is responsible for forelimb-girdle muscular anomaly and developed the genetic diagnosis systems for this disorder by PCR-RFLP method. For other disorders including short mandible and renal agenesis, we have collected samples of affected animals and performed linkage analysis using these samples. As a result, the locus for short mandible and renal agenesis was mapped on a particular region of bovine chromosome. These finding can be used to develop genetic diagnosis system for this disorder.
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