A study on the suppression protocol on proliferation of solid tumors regulating cancer stem cells and neo-vascularization

• Project/Area Number: 20248032
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: MATSUDA Hiroshi Tokyo University of Agriculture and Technology, 大学院・農学研究院, 教授 (80145820)
• Co-Investigator(Kenkyū-buntansha): TANAKA Akane 東京農工大学, 大学院・農学研究院, 准教授 (80418673) ; OHMORI Keitaro 東京農工大学, 大学院・農学研究院, 助教 (20466915)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥44,200,000 (Direct Cost: ¥34,000,000、Indirect Cost: ¥10,200,000); Fiscal Year 2010: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000); Fiscal Year 2009: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000); Fiscal Year 2008: ¥26,000,000 (Direct Cost: ¥20,000,000、Indirect Cost: ¥6,000,000)
• Keywords: 低酸素反応性因子 / 癌 / 遺伝子治療 / 治療

Research Abstract

In a malignant solid carcinoma, proliferation of cancer cells are sometimes accelerated after a surgery and radiation therapy and a cancer may recur in a short term. It is thought that a cancer stem cell of the drug resistance and the high vascularization inducibility bring the rapid expansion of the cancer tissue, but the control method is not yet found. Based on the author's past research on mast cell tumors, we evaluated a silencing therapy or a gene control therapy for the endogenous inhibitors for vascularization and hypoxia inducible factors which controlled neo-vascularization. We developed effective therapy for controlling neo-vascularization. In addition, we elucidated mechanism of the drug resistance of cancer stem cells and obtained fundamental researche data for establishment of a novel therapy.

Research Products

• [Journal Article] PPARgamma-mediated suppression of dendritic cell function prevents the onset of atopic dermatitis in NC/NgaTnd mice. (2011)
  • Author(s): Jung K, Tanaka A, Fujita II, Matsuda A, Oida K, Karasawa K, Okamoto N, Ohmori K, Jee Y, Shin T, Matsuda H
  • Journal Title: J.Allergy Clin.Immunol. 127 Pages: 420-429
• [Journal Article] Effect of NGF on the motility and acrosome reaction of golden hamster spermatozoa in vitro. (2010)
  • Author(s): Jin W, Tanaka A, Watanabe G, Matsuda II, Taya K
  • Journal Title: J.Reprod.Develop. 56 Pages: 437-443
  • NAID: 10026621774
• [Journal Article] A novel NF-B inhibitor improves glucocorticoid sensitivity of canine neoplastic lymphoid cells by up-regulating expression of glucocorticoid receptors. (2010)
  • Author(s): Matsuda A, Tanaka A, Muto S, Ohmori K, Furusaka T, Jung K, Karasawa K, Okamoto N, Oida K, Itai A, Matsuda H
  • Journal Title: Res.Vet.Sci. 89 Pages: 378-382
• [Journal Article] Pilot evaluation of the efficacy of shampoo treatment with ultra-pure soft water for canine pruritus. (2010)
  • Author(s): Ohmori R, Tanaka A, Makita Y, Takai M, Yoshinari Y, Matsuda H
  • Journal Title: Vet.Dermatol. 21 Pages: 477-483
• [Journal Article] A novel NF-κB inhibitor improves glucocorticoid sensitivity of canine neoplastic lymphoid cells by up-regulating expression of glucocorticoid receptors. (2010)
  • Author(s): Matsuda A, Tanaka A, Muto S, K.Ohmori, T.Furusaka, K.Jung, K.Karasawa, N.Okamoto, K.Oida, A.Itai, H.Matsuda
  • Journal Title: Research in Veterinary Science Volume: 89 Pages: 378-382
  • Peer Reviewed
• [Journal Article] Pilot evaluation of the efficacy of shampoo treatment with ultra-pure soft water for canine pruritus. (2010)
  • Author(s): Ohmori K, Tanaka A, Makita Y, Takai M, Yoshinari, Y, Matsuda H.
  • Journal Title: Veterinary Dermatology (In press)
  • Peer Reviewed
• [Journal Article] A novel NF-kappaB inhibitor improves glucocorticoid sensitivity of canine neoplastic lymphoid cells by up-regulating expression of glucocorticoid receptors. (2010)
  • Author(s): Matsuda A, Tanaka A, Muto S, Ohmori K, Furusaka T, Jung K, Karasawa K, Okamoto N, Oida K, Itai A, Matsuda H.
  • Journal Title: Research in Veterinary Science (In press)
  • Peer Reviewed
• [Journal Article] Oral supplementation with Lactobacillus rhamnosus CGMCC 1.3724 prevents development of atopic dermatitis in NC/NgaTnd mice possibly by modulating local production of IFN-gamma. (2009)
  • Author(s): Tanaka A, Jung K, Benyacoub J, Prioult G, Okamoto N, Ohmori K, Blum S, Mercenier A, Matsuda H.
  • Journal Title: Experimental Dermatology 18(12) Pages: 1022-1027
  • Peer Reviewed
• [Journal Article] Establishment of a novel high-affinity IgE receptor-positive canine mast cell 1ine with wild type c-kit receptors (2008)
  • Author(s): Amagai Y., Tanaka A., Matsuda H.
  • Journal Title: Biochem Biophys Res Commun. 366 Pages: 857-61
  • Peer Reviewed
• [Journal Article] Identification of c-kit mutations-independent neoplastic cell proliferation of canine mast cells. (2008)
  • Author(s): Ohmori K, Kawarai S, Yasuda N, Tanaka A, Matsuda H, Nishimura R, Sasaki N, Tsujimoto H, Masuda K.
  • Journal Title: Vet Immuno1 Immunopatho1. 126 Pages: 43-53
  • Peer Reviewed
• [Journal Article] Prophylacic effect of oral administration of Lactobacillus johnsonii NCC533 (Lai)during weaning period on atopic dermatitis in NC/NgaTnd mice (2008)
  • Author(s): Tanaka A., Fukushima Y., Benyacoub J., Blum s., Matsuda H.
  • Journal Title: Eur. J. Dermatol 18 Pages: 136-140
  • Peer Reviewed
• [Journal Article] Consecutive daily low-dose S-1 adjuvant chemotherapy after radical treatment for squamous cell carcinoma in head and neck cancer.
  • Author(s): Furusaka T, Tanaka A, Matsuda H, Ikeda M
  • Journal Title: Acta Oto-Laryngologica In press.
• [Journal Article] Retinal degeneration and rdl mutation in NC/NgaTnd mice, a human atopic dermatitis model.
  • Author(s): Karasawa K, Tanaka A, Jung K, Matsuda A, Okamoto N, Oida K, Ebihara N, Ohmori K, Matsuda H
  • Journal Title: Curr.Eye Res. In press.
• [Journal Article] Matsuda IC Patterns of aquaporin expression in the canine eye.
  • Author(s): Karasawa K, Tanaka A, Jung K, Matsuda A, Okamoto N, Oida K, Ohmori K
  • Journal Title: Vet.J. In press.
• [Presentation] 急性リンパ性白血病細胞におけるグルココルチコイド耐性獲得機構と転写因子NF-κBの関連性 (2010)
  • Author(s): Matsuda A, Tanaka A, Ohmori K, Matsuda H
  • Organizer: 第48回日本癌治療学会
  • Year and Date: 2010-10-30
• [Presentation] 急性リンパ性白血病細胞におけるグルココルチコイド耐性獲得機構と転写因子NF-κBの関連性 (2010)
  • Author(s): Matsuda A, Tanaka A, Ohmori K, Matsuda H
  • Organizer: 第48日本癌治療学会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-30
• [Presentation] ヒト中皮腫細胞における腫瘍性増殖制御分子としての転写因子NF-κBの役割 (2010)
  • Author(s): Nishikawa S, Tanaka A, Ohm・ri K, MatsudaH
  • Organizer: 第150日本獣医学会
  • Year and Date: 2010-09-16
• [Presentation] ヒト中皮腫細胞における腫瘍性増殖制御分子としての転写因子NF-κBの役割 (2010)
  • Author(s): Nishikawa S, Tanaka A, Ohmori K, Matsuda H
  • Organizer: 第150日本獣医学会
  • Place of Presentation: 帯広
  • Year and Date: 2010-09-16
• [Presentation] Inhibition of nuclear factor-KQ activity restored glucocarticoid sensitivity in lymphocytes and mast cells. (2010)
  • Author(s): Matsuda A, Tanaka A, Ohmori K, Matsuda H.
  • Organizer: 第14回国際免疫学会議
  • Year and Date: 2010-08-27
• [Presentation] Inhibition of nuclear factor-κB activity restored glucocorticoid sensitivity in lymphocytes and mast cells. (2010)
  • Author(s): Matsuda A, Tanaka A, Ohmori K, Matsuda H
  • Organizer: 第14回国際免疫学会議
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-27
• [Presentation] Inhibition of NF-κBactivity restored glucocorticoid sensitivity in lymphocytes and mast cells. (2010)
  • Author(s): Matsuda A, Tanaka A, Ohmori K, Matsuda H
  • Organizer: 第9回国際獣医免疫シンポジウム
  • Year and Date: 2010-08-18
• [Presentation] Inhibition of NF-κB activity restored glucocorticoid sensitivity in lymphocytes and mast cells. (2010)
  • Author(s): Matsuda A, Tanaka A, Ohmori K, Matsuda H
  • Organizer: 第9回国際獣医免疫シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2010-08-18
• [Presentation] Inhibition of nuclear factor-κB activity restored glucocarticoid sensitivity in lymphocytes and mast cells. (2010)
  • Author(s): Matsuda A, Tanaka A, Ohmori K, Matsuda H.
  • Organizer: 第29回欧州アレルギー臨床免疫学会
  • Year and Date: 2010-06-07
• [Presentation] Inhibition of nuclear factor-κB activity restored glucocorticoid sensitivity in lymphocytes and mast cells. (2010)
  • Author(s): Matsuda A, Tanaka A, Ohmori K, Matsuda H
  • Organizer: 第29回欧州アレルギー臨床免疫学会
  • Place of Presentation: イギリス・ロンドン
  • Year and Date: 2010-06-07
• [Presentation] ガン治療の基礎と最前線-特に分子標的治療法について- (2009)
  • Author(s): Hiroshi Matsuda
  • Organizer: 第40回日本比較臨床医学会
  • Place of Presentation: 招待講演
  • Year and Date: 2009-11-29
• [Presentation] ガン治療の基礎と最前線 -特に分子標的治療法について- (2009)
  • Author(s): 松田浩珍
  • Organizer: 第40回日本比較臨床医学会
  • Place of Presentation: 埼玉県東松山市
  • Year and Date: 2009-11-29
• [Presentation] Update・f NC/NgaTnd mice as an atopic dermatitis model. (2009)
  • Author(s): Hir・shi Matsuda
  • Organizer: International Workshop for the Study of Itch
  • Place of Presentation: 招待講演
  • Year and Date: 2009-10-27
• [Presentation] Update of NC/NgaTnd mice as an atopic dermatitis model (2009)
  • Author(s): 松田浩珍
  • Organizer: International Workshop for the Study of Itch
  • Place of Presentation: 東京
  • Year and Date: 2009-10-27
• [Presentation] リンパ腫/白血病細胞におけるグルココルチコイド耐性獲得機構における転写因子NF-κBの関与 (2009)
  • Author(s): Matsuda A, Tanaka A, Ohmori K, Matsuda H
  • Organizer: 第148回日本獣医学会学術集会
  • Year and Date: 2009-09-26
• [Presentation] リンパ腫/白血病細胞におけるグルココルチコイド耐性獲得機構における転写因子NF-κBの関与 (2009)
  • Author(s): 松田彬、大森啓太郎、田中あかね、松田浩珍
  • Organizer: 第148回日本獣医学会学術集会
  • Place of Presentation: 鳥取県鳥取市
  • Year and Date: 2009-09-26
• [Presentation] Does a strategy that interferes with the NF-kappa B pathway bring a new insight into the hematopoietic tumor therapy? (2008)
  • Author(s): Tanaka A., Ohmori K., Matsuda H.
  • Organizer: 第13回世界先端ガン治療学会・第11回国際分子医学シンポジウム
  • Place of Presentation: ギリシヤ・クレタ島
  • Year and Date: 2008-10-10
• [Presentation] ヒト乳がん細胞株における腫瘍化抑制標的分子の検討 (2008)
  • Author(s): Oida K, Tanaka A, Ohmori K, Matsuda H
  • Organizer: 第146回日本獣医学会学術集会
  • Year and Date: 2008-09-25
• [Presentation] Int6遺伝子サイレンシングによる後肢虚血モデルにおける末梢血管の誘導と歩行機能の改善 (2008)
  • Author(s): Okamoto K, Tanaka A, Ohmori K, Matsuda H.
  • Organizer: 第146回日本獣医学会学術集会
  • Year and Date: 2008-09-25
• [Presentation] ヒト乳がん細胞株における腫瘍化抑制標的分子の検討 (2008)
  • Author(s): 種田久美子, 田中あかね, 大森啓太郎, 松田浩珍
  • Organizer: 第146回日本獣医学会
  • Place of Presentation: 宮崎県宮崎市
  • Year and Date: 2008-09-25
• [Presentation] Int6遺伝子サイレンシングによる後肢虚血モデルにおける末梢血管の誘導と歩行機能の改善 (2008)
  • Author(s): 岡本典子, 田中あかね, 大森啓太郎, 松田浩珍
  • Organizer: 第146回目本獣医学会
  • Place of Presentation: 宮崎県宮崎市
  • Year and Date: 2008-09-25
• [Remarks] ホームページ等
  • URL: http://www.tuat.ac.jp/~molpath/
• [Remarks] 獣医分子病態治療学研究室ホームページ
• [Remarks]
  • URL: http://www.tuat.ac.jp/~mol_path/index.html
• [Remarks]
  • URL: http://www.tuat.ac.jp/~mol_path/index.html
• [Remarks] 獣医分子病態治療学研究室ホームページ
  • URL: http://www.tuat.ac.jp/~mol_path/
• [Patent(Industrial Property Rights)] 移植用細胞シートの製造方法、移植用細胞シート、および移植用細胞シートを用いる治療方法 (2010)
  • Inventor(s): 田中あかね、松田浩珍
  • Industrial Property Rights Holder: 田中あかね、松田浩珍
  • Industrial Property Number: 2010-192683
  • Filing Date: 2010-08-30