| Budget Amount *help |
¥49,660,000 (Direct Cost: ¥38,200,000、Indirect Cost: ¥11,460,000)
Fiscal Year 2012: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2011: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2010: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2009: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2008: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
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| Research Abstract |
We proposed a fundamental paradigm"GlcNAc cycle" that living cell regulates biosynthesis and catabolism of glycans in response to pathological changes. We could grasp the dynamic cellular information by analyzing the each step in glycosylation. Nucleotide sugars level were simultaneously determined by ion-pair HPLC. Mass isotopomer analysis of metabolically labeled nucleotide sugars and N- and O-glycans revealed the UDP-GlcNAc metabolisms. We also developed a HPLC method for various GlcNAc transferases activities, and found the endogenous inhibitory factor for GlcNAc transferase-IX (GnT-IX) in Neuro2a cells. Brain-specific expression of the GnT-IX was also regulated by epigenetic histone modifications. In addition, GnT-III compensated for the loss of core fucose functions. These data suggested that glycosylation is regulated by multiple stages in cellular GlcNAc metabolisms.
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