Liver transplantation with novel strategy using the engineered chimeric liver as alternative graft

• Project/Area Number: 20249058
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General surgery
• Research Institution: Kyoto University
• Principal Investigator: UEMOTO Shinji Kyoto University, 医学研究科, 教授 (40252449)
• Co-Investigator(Kenkyū-buntansha): KOBAYASHI Eiji 自治医科大学, 医学部, 客員教授 (00245044)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥25,740,000 (Direct Cost: ¥19,800,000、Indirect Cost: ¥5,940,000); Fiscal Year 2010: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2009: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000); Fiscal Year 2008: ¥11,700,000 (Direct Cost: ¥9,000,000、Indirect Cost: ¥2,700,000)
• Keywords: 移植外科学 / 肝臓移植 / キメラ肝臓

Research Abstract

To solve the serious shortage of liver grafts, the feasibility of chimeric liver as an alternative liver graft has been investigated. The chimeric liver formed with rat hepatic parenchyma and mouse non-parenchyma was harvested from scaffold mouse and transplanted into rat recipient, followed by the immunosuppressive protocols. We have found, with appropriate immunosuppression, graft survival was significantly prolonged and graft tissues were maintained better. Moreover, we found its albumin production for 4 months. We have shown the possibility of the strategy using chimeric liver graft.

Research Products

• [Journal Article] Development of a portocaval shunt using a small intestinal segment in rats. (2010)
  • Author(s): T.Hata, S.Uemoto, E.Kobayashi, et. al.
  • Journal Title: Microsurgery Vol30(4) Pages: 302-306
  • Peer Reviewed
• [Presentation] Auxiliary Transplantation of the chimeric liver graft in mouse and rat combination and evaluation of the long-term survival and proliferative capacity of the transplanted graft in the recipient rat. (2010)
  • Author(s): 畑俊行, 小林英司, 上本伸二, et al.
  • Organizer: 23rd International Congress of The transplantation Society.
  • Place of Presentation: Vancouver, Canada.
  • Year and Date: 2010-08-18
• [Presentation] Auxiliary Transplantation of the chimeric liver graft in mouse and rat combination and evaluation of the long-term aurvival aud proliferative capacity of the transplanted graft in the recipient rat (2010)
  • Author(s): Toshiyuki Hata, et al.
  • Organizer: 23^<rd> International Congress of The transplantation Society
  • Place of Presentation: カナダ・バンクーバー
  • Year and Date: 2010-08-18
• [Presentation] Repopulation of hepatocytes simultaneously transplanted into the liver graft in the rat liver transplantation. (2010)
  • Author(s): K.Endo, T.Hata, S.Uemoto, et al.
  • Organizer: 23rd International Congress of The transplantation Society.
  • Place of Presentation: Vancouver, Canada.
  • Year and Date: 2010-08-17
• [Presentation] Replacement of the vascular endothelium in the mouse liver by the xenogeneic bone-marrow cells derived from the rat. (2010)
  • Author(s): K.Jobara, T.Hata, S.Uemoto, et al.
  • Organizer: 23rd International Congress of The transplantation Society.
  • Place of Presentation: Vancouver, Canada.
  • Year and Date: 2010-08-17
• [Presentation] Repopulation of hepatocytes simultaneously transplanted into the liver graft in the rat liver transplantation (2010)
  • Author(s): Kosuke Endo, et al.
  • Organizer: 23^<rd> International Congress of The transplantation Society
  • Place of Presentation: カナダ・バンクーバー
  • Year and Date: 2010-08-17
• [Presentation] Replacement of the vascular endothelium in the mouse liver by the xenogeneic bone-marrow cells derived from the rat (2010)
  • Author(s): Kanta Jobara, et al.
  • Organizer: 23^<rd> International Congress of The transplantation Society
  • Place of Presentation: カナダ・バンクーバー
  • Year and Date: 2010-08-17
• [Presentation] Development of regerated chimeric liver grafts in mouse and rat combination and long-term evaluation of their potential after auxiliary transplantation. (2009)
  • Author(s): 畑俊行, 小林英司, 上本伸二, et al.
  • Organizer: 14^<th> Congress of the European Society for Organ Transplantation.
  • Place of Presentation: Paris, France.
  • Year and Date: 2009-08-30
• [Presentation] Development of regerated chimeric liver grafts in mouse and rat combination and long-term evaluation of their potential after auxiliary transplantation. (2009)
  • Author(s): Toshiyuki Hata, et al.
  • Organizer: 14^<th> Congress of the European Society for Organ Transplantation
  • Place of Presentation: フランス・パリ
  • Year and Date: 2009-08-30
• [Presentation] Long-term function of transplanted chimeric liver with autologous hepatocyte and xenobiotic scaffold in mouse and rat combination. (2009)
  • Author(s): 畑俊行, 小林英司, 上本伸二, et al.
  • Organizer: American Transplant Congress 2009.
  • Place of Presentation: Boston, USA.
  • Year and Date: 2009-06-03
• [Presentation] Long-term function of transplanted chimeric liver with autologous hepatocyte and xenobiotic scaffold in mouse and rat combination. (2009)
  • Author(s): Toshiyuki Hata, et al.
  • Organizer: American Transplant Congress 2009
  • Place of Presentation: アメリカ・ボストン
  • Year and Date: 2009-06-03
• [Presentation] キメラ技術を用いた再生肝臓移植の検討 (2009)
  • Author(s): 畑俊行、小林英司、上本伸二, 他.
  • Organizer: 第12回日本異種移植研究会
  • Place of Presentation: 鹿児島
  • Year and Date: 2009-03-09
• [Presentation] Surgical Technique in two models of auxiliary liver transplantation from mice to rats. (2008)
  • Author(s): 畑俊行, 上本伸二, 小林英司, et al.
  • Organizer: The 9th Congress of the International Society for Experimental Microsurgery.
  • Place of Presentation: 上海,中国
  • Year and Date: 2008-10-31
• [Presentation] Surgical Technique in two models of auxiliary liver transplantation from mice to rats (2008)
  • Author(s): Toshiyuki Hata, Junji Iwasaki, Shuji Hishikawa, Shinji Uemoto
  • Organizer: The 9th Congress of the International Society for Expperimental Microsurgery
  • Place of Presentation: 中国・上海
  • Year and Date: 2008-10-31
• [Presentation] 小腸をscaffoldに利用した臓器再生とキメラ技術の応用. (2008)
  • Author(s): 畑俊行、上本伸二、小林英司, 他.
  • Organizer: 第44回日本移植学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2008-09-21
• [Presentation] 小腸をscaffoldに利用した臓器再生とキメラ技術の応用 (2008)
  • Author(s): 畑俊行, 岩崎純治, 上本伸二, 小林英司
  • Organizer: 第44回日本移植学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2008-09-21
• [Presentation] Transplantation of chimeric liver with autologous hepatocyte and xenobiotic scaffold in mouse and rat combination. (2008)
  • Author(s): 畑俊行, 上本伸二, 小林英司, et al.
  • Organizer: American Transplant Congress 2008.
  • Place of Presentation: Toronto, Canada.
  • Year and Date: 2008-06-02
• [Presentation] Transplantation of chimeric liver with autologous hepatocyte and xenobiotic scaffold in mouse and rat combination (2008)
  • Author(s): Toshiyuki Hata, Shinji Uemoto, Eiji Kobayashi
  • Organizer: American Transplant Congress 2008
  • Place of Presentation: カナダ・トロント
  • Year and Date: 2008-06-02
• [Presentation] 自己肝細胞によるキメラ肝臓移植 (2008)
  • Author(s): 畑俊行、上本伸二、小林英司, 他.
  • Organizer: 第108回日本外科学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2008-05-15
• [Remarks] ホームページ等
  • URL: http://hbptsurgery.kuhp.kyoto-u.ac.jp/kantansui/index.html