Characterization of sarcoma stem cells and development of the stem cell-targeting agents utilizing viral engineering
| Project/Area Number |
20249065
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Research Institute, Osaka Medical Center for Cancer and Cardiovascular Disaeses |
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Principal Investigator |
TAKAHASHI Katsuhito Research Institute, Osaka Medical Center for Cancer and Cardiovascular Disaeses, 研究所, 部長 (40211338)
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| Co-Investigator(Kenkyū-buntansha) |
TOGUCHIDA Junya 京都大学, 再生医科学研究所, 教授 (40273502)
YAMAMURA Hisako 地方独立行政法人大阪府立病院機構大阪府立成人病センター(研究所), 研究所, 研究員 (50342994)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥49,790,000 (Direct Cost: ¥38,300,000、Indirect Cost: ¥11,490,000)
Fiscal Year 2010: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2009: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2008: ¥32,890,000 (Direct Cost: ¥25,300,000、Indirect Cost: ¥7,590,000)
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| Keywords | 肉腫 / 幹細胞 / 腫瘍溶解性ウイルス |
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| Research Abstract |
The purpose of this study is to concentrate the stem (tumor-initiating) cell fraction of sarcoma, and then to develop stem cell-targeted agent, utilizing cell-targeting technologies with oncolytic virus. Several lines of highly tumorigenic osateosarcoma were isolated. Tumor-initiating cells from gastrointestinal stromal tumor, sarcoma-like tumor, were concentrated into the cellular fraction characterized with the cell surface marker CD133(Low/-) in vitro. Those cells were shown to be silenced in the hypoxic region of tumor, and resistant to Imatinib treatment. Tumor-initiating cells from sarcomatous mesothelioma were concentrated into the cellular fraction with CD133 (Low/-) and CD44(+). We showed that our HSV-1 oncolytic virus d12ODDΔRR targeting hypoxic microenvironment of tumor successfully destroyed tumor-initiating cells from stromal tumor and mesothelioma.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Mediastinal synovial sarcoma. Aoyama T, Okamoto T, Fukiage K, Otsuka S, Furu M, Ito K, Jin Y, Ueda M, Nagayama S, Nakayama T, Nakamura T, Toguchida J. Histone modifiers, YY1 and p300, regulate the expression of cartilage -specific gene, chondromodulin-I, in mesenchymal stem cells.2010
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[Journal Article] Histone modifiers, YY1 and p300, regulate the expression of cartilage-specific gene, chondromodulin-I, in mesenchymal stem cells.2010
Author(s)
Aoyama T, Okamoto T, Fukiage K, Otsuka S, Furu M, Ito K, Jin Y, Ueda M, Nagayama S, Nakayama T, Nakamura T, Toguchida J.
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Journal Title
J Biol Chem
Volume: 285
Pages: 29842-29850
Related Report
Peer Reviewed
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[Journal Article] A novel method to isolate mesenchymal stem cells from bone marrow in a closed system using a device made by nonwoven fabric.2010
Author(s)
Ito K, Aoyama T, Fukiage K, Otsuka S, Furu M, Jin Y, Nasu A, Ueda M, Kasai Y, Ashihara E, Kimura S, Maekawa T, Kobayashi A, Yoshida S, Niwa H, Otsuka T, Nakamura T, Toguchida J.
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Journal Title
Tissue Eng Part C Methods. 16
Pages: 81-91
Related Report
Peer Reviewed
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