Secretion mechanism of pathogenic proteases and adhesions in the periodontal pathogen Porphyromonas gingivalis.
Project/Area Number |
20249073
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Morphological basic dentistry
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Research Institution | Nagasaki University |
Principal Investigator |
NAKAYAMA Koji Nagasaki University, 大学院医・歯薬学総合研究科, 教授 (80150473)
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Co-Investigator(Renkei-kenkyūsha) |
NAITO Mariko 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (20244072)
SHOJI Mikio 長崎大学, 大学院・医歯薬学総合研究科, 助教 (10336175)
SATO Keiko 長崎大学, 大学院・医歯薬学総合研究科, 助教 (70410579)
YUKITAKE Hideharu 長崎大学, 大学院・医歯薬学総合研究科, 技術職員 (30380984)
HIRAKAWA Hideki かずさDNA研究所, 植物ゲノム情報研究室, 研究員 (80372746)
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Project Period (FY) |
2008 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥48,490,000 (Direct Cost: ¥37,300,000、Indirect Cost: ¥11,190,000)
Fiscal Year 2010: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2009: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
Fiscal Year 2008: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
|
Keywords | 歯周病 / Porphyromonas gingivalis / gingipain / タンパク分泌機構 / Flavobacterium johnsoniae / 滑走運動 |
Research Abstract |
Porphyromonas gingivalis secretes strong proteases called gingipains that are implicated in periodontal pathogenesis. Comparative genome analysis and genetic experiments revealed proteins involved in gingipain secretion. These proteins were similar in sequence to Flavobacterium johnsoniae gliding motility proteins. Disruption of the F. johnsoniae porT ortholog resulted in defects in motility and chitinase secretion, providing a link between a unique protein translocation system and a motility apparatus in members of the Bacteroidetes phylum.
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Report
(4 results)
Research Products
(50 results)
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[Journal Article] Characterization of hemin-binding protein 35 (HBP35) in Porphyromonas gingivalis : its cellular distribution, thioredoxin activity and role in heme utilization.2010
Author(s)
Shoji M, Shibata Y, Shiroza T, Yukitake H, Peng B, Chen Y-Y, Sato K, Naito M, Abiko Y, Reynolds EC, Nakayama K
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Journal Title
BMC Microbiol 10(online journal)
Pages: 152
Related Report
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[Journal Article] Neutralization of toxic haem by Porphyromonas gingivalis haemoglobin receptor.2010
Author(s)
Nhien NT, Huy NT, Naito M, Oida T, Uyen DT, Huang M, Kikuchi M, Harada S, Nakayama K, Hirayama K, Kamei K
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Journal Title
J.Biochem. 147
Pages: 317-325
Related Report
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[Journal Article] Characterization of hemin-binding protein 35 (HBP35) in Porphyromonas gingivalis : its cellular distribution, thioredoxin activity and role in heme utilization2010
Author(s)
Shoji M, Shibata Y, Shiroza T, Yukitake H, Peng B, Chen Y-Y, Sato K, Naito M, Abiko Y, Reynolds EC, Nakayama K
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Journal Title
BMC Microbiology
Volume: 10(online journal)
Pages: 152
Related Report
Peer Reviewed
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[Journal Article] Neutralization of toxic haem by Porphyromonas gingivalis haemoglobin receptor2010
Author(s)
Nhien NT, Huy NT, Naito M, Oida T, Uyen DT, Huang M, Kikuchi M, Harada S, Nakayama K, Hirayama K, Kamei K
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Journal Title
J.Biochem
Volume: 147
Pages: 317-325
Related Report
Peer Reviewed
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[Journal Article] Determination of the genome sequence of Porphyromonas gingivalis strain ATCC33277 and genomic comparison with strain W83 revealed extensive genome rearrangements in P.gingivalis.2008
Author(s)
Naito M, Hirakawa H, Yamashita A, Ohara N, Shoji M, Yukitake H, Nakayama K, Toh H, Yoshimura F, Kuhara S, Hattori M, Hayashi T, Nakayama K
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Journal Title
DNA Res 15
Pages: 215-225
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