| Budget Amount *help |
¥39,910,000 (Direct Cost: ¥30,700,000、Indirect Cost: ¥9,210,000)
Fiscal Year 2010: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2009: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2008: ¥22,490,000 (Direct Cost: ¥17,300,000、Indirect Cost: ¥5,190,000)
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| Research Abstract |
We developed a novel fluorescence-labeled cancer-targeted liposome coupled to sindbis virus proteins (SIN hybrid liposome) on the surface that can be used for imaging tumors and optimized for a bio-distribution analysis and an intra-operative navigation system. SIN hybrid liposome was prepared by mixing the pegylated liposome and UV-inactivated Sindbis virus protein that were purified by an ultracentrifuge. The structure of SIN hybrid liposome was observed by a Transmission Electron Microscope. When cancer cells were incubated with SIN hybrid or non-SIN hybrid rhodamin labeled liposomes encapsulating FITC-dextran, both conforcal microscopy and flow cytometry revealed that a large number of rhodamin fluorescence on the cell membrane and FITC in the cytoplasm were detected in the cancer cells treated with SIN hybrid liposomes compared with non-SIN hybrid liposomes. Furthermore, each liposome was systemically injected in tumor bearing mouse model and imaged for distribution using IVIIS lumina systems. SIN hybrid liposome showed distribution and accumulation at the tumor site preferentially and improved the signal-to-noise ration.
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