Metagenomic Analysis of Oral Microbiota from Normal Subjects and Patients with Periodontal Disease

• Project/Area Number: 20249082
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Periodontal dentistry
• Research Institution: Aichi Gakuin University
• Principal Investigator: NOGUCHI Toshihide Aichi Gakuin University, 歯学部, 教授 (50014262)
• Co-Investigator(Kenkyū-buntansha): KOJIMA Toshio 浜松医科大学, 実験実習器機センター, 准教授 (00311340) ; MORITA Hidetoshi 麻布大学, 獣医学部, 准教授 (70257294) ; HAYASHI Junichiro 愛知学院大学, 歯学部, 講師 (30350937)
• Co-Investigator(Renkei-kenkyūsha): FUKUDA Mitsuo 愛知学院大学, 歯学部, 教授 (40156790) ; ISHIHARA Yuichi 愛知学院大学, 歯学部, 准教授 (50261011) ; KAMEI Hidehiko 愛知学院大学, 歯学部, 助教 (50421243) ; HATTORI Masahira 東京大学, 新領域創成科学研究科, 教授 (70175537) ; TOH Hidehiro 理化学研究所, 計算生命科学研究センター, 研究員 (10353468)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥48,360,000 (Direct Cost: ¥37,200,000、Indirect Cost: ¥11,160,000); Fiscal Year 2010: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000); Fiscal Year 2009: ¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000); Fiscal Year 2008: ¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
• Keywords: メタゲノム解析 / 口腔細菌叢 / 歯周病関連細菌 / 歯周炎 / ゲノムDNA抽出 / 歯肉縁上プラーク / 歯肉縁下プラーク / 16SrRNA遺伝子解析

Research Abstract

A metagenomic analysis of dental plaque microbiota obtained from periodontal healthy subjects and periodontitis patients was performed. The deep sequencing of the seven DNA samples detected total of 996 species and revealed substantially greater microbial diversity in the dental plaque than previously known. The functional binning profiles of these samples were compared with other environmental metagenome datasets such as human gut microbiota. Enrichments of genes in the categories of 'Defense mechanism' and 'carbon hydrate transport and metabolism' were observed in plaque samples.

Research Products

• [Journal Article] Bifidobacterium kashiwanohense sp.nov., isolated from healthy infant faeces. (2010)
  • Author(s): Morita H, Nakano A, Onoda H, Toh H, Oshima K, Takami H, Murakami M, Fukuda S, Takizawa T, Kuwahara T, Ohno H, Tanabe S, Hattori M.
  • Journal Title: Int.J.Syst.Evol.Microbiol.(電子版)
  • Peer Reviewed
• [Journal Article] The effect of horizontal X-ray beam angulation on the detection of furcation defects of mandibular first molars in intraoral radiology. (2010)
  • Author(s): Hishikawa T, Izumi M, Naitoh M, Furukawa M, Yoshinari N, Kawase H, Matsuoka M, Noguchi T, Ariji E.
  • Journal Title: Dentomaxillofac Radiol. 39 Pages: 85-90
  • Peer Reviewed
• [Journal Article] MiR-148a attenuates paclitaxel-resistance of hormone-refractory, drug-resistant prostate cancer PC3 cells by regulating MSK1 expression. (2010)
  • Author(s): Fujita Y, Kojima K, Ohhashi R, Hamada N, Nozawa Y, Kitamoto A, Sato A, Kondo S, Kojima T, Deguchi T, Ito M.
  • Journal Title: J.Biol.Chem. 285 Pages: 19076-84
  • Peer Reviewed
• [Journal Article] Lactobacillus equicursoris sp.nov.isolated from the faeces of a thoroughbred racehorse. (2010)
  • Author(s): Morita H, Shimazu M, Shiono H, Toh H, Nakajima F, Akita H, Takagi M, Takami, H, Murakami M, Masaoka T, Tanabe S., Hattori M.
  • Journal Title: Int.J.Syst.Evol.Microbiol. 60 Pages: 109-112
  • Peer Reviewed
• [Journal Article] Lactobacillus equicursoris sp.nov.isolated from the faeces of a thoroughbred racehorse (2010)
  • Author(s): Morita, H.
  • Journal Title: Int.J.Syst.Evol.Microbiol. Volume: 60 Pages: 109-112
  • Peer Reviewed
• [Journal Article] Bifidobacterium kashiwanohense sp.nov., isolated from healthy infant faeces (2010)
  • Author(s): Morita, H.
  • Journal Title: Int.J.Syst.Evol.Microbiol. Volume: (電子版)
  • Peer Reviewed
• [Journal Article] The effect of horizontal X-ray beam angulation on the detection of furcation defects of mandibular first molars in intraoral radiology. (2010)
  • Author(s): Hishikawa, T.Noguchi, T.
  • Journal Title: Dentomaxillofac Radiol Volume: 39 Pages: 85-90
  • Peer Reviewed
• [Journal Article] MiR-148a attenuates paclitaxel-resistance of hormone-refractory, drug-resistant prostate cancer PC3 cells by regulating MSK 1 expression. (2010)
  • Author(s): Fujita, Y.Kojima, T.
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 19076-84
  • Peer Reviewed
• [Journal Article] Complete genome sequence of probiotic Lactobacillus rhamnosus ATCC 53103. (2009)
  • Author(s): Morita H, Toh H, Oshima K, Murakami M, Taylor TD, Igimi S, Hattori M.
  • Journal Title: J.Bacteriol. 191 Pages: 7630-7631
  • Peer Reviewed
• [Journal Article] High-resolution analysis of aberrant regions in autosomal chromosomes in human leukemia THP-1 cell line. (2009)
  • Author(s): Adati N, Huang MC, Suzuki T, Suzuki H, Kojima T.
  • Journal Title: BMC research notes 2 Pages: 153-159
  • Peer Reviewed
• [Journal Article] Degradation of vascular endothelial thrombomodulin by arginine-and lysin-specific cysteine proteases from Porphyromonas gingivalis. (2009)
  • Author(s): Inomata M, Ishihara Y, Matsuyama T, Murayama I, Noguchi T, Matsushita K.
  • Journal Title: J.Periodontol. 8 Pages: 1511-1517
  • Peer Reviewed
• [Journal Article] Complete genome sequence of probiotic Lactobacillus rhamnosus ATCC 53103 (2009)
  • Author(s): Morita H, Toh H, Oshima K, Murakami M, Taylor TD, Igimi S, Hattori M
  • Journal Title: J.Bacteriol. 191 Pages: 7630-7631
  • Peer Reviewed
• [Journal Article] High-resolution analysis of aberrant regions in autosomal chromosomes in human leukemia THP-1 cell line (2009)
  • Author(s): Adati N, Huang MC, Suzuki T, Suzuki H, Kojima T.
  • Journal Title: BMC research notes 2 Pages: 153-159
  • Peer Reviewed
• [Journal Article] Degradation of vascular endothelial thrombomodulin by arginine-and lysin-specific cysteine proteases from Porphyromonas gingivalis (2009)
  • Author(s): Inomata M, Ishihara Y, Matsuyama T, Murayama I, Noguchi T, Matsushita K
  • Journal Title: J.Periodontol. 80 Pages: 1511-1517
  • Peer Reviewed
• [Presentation] ヒト歯周病細菌叢のメタゲノム解析 (2011)
  • Author(s): 林潤一郎,近藤伸二、亀井英彦、森田英利、足立直樹、福田光男、Todd D Taylor、石原裕一、小島俊男、野口俊英
  • Organizer: 第5回日本ゲノム微生物学会
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-14
• [Presentation] Metagenomic analysis of the dental plaque (2010)
  • Author(s): Hayashi J, Kondo S, Morita H, Kamei H, Fukuda M, Ishihara Y, Kojima T, Noguchi T
  • Organizer: 96^<th> American Academy of Periodotology Annual Meeting
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2010-11-02
• [Presentation] METAGENOMIC ANALYSIS OF THE DENTAL PLAQUE (2010)
  • Author(s): 林潤一郎
  • Organizer: 96^<th> American Academy of Periodotology Annual Meeting
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2010-11-02
• [Presentation] 腸内細菌叢のメタゲノム解析における細菌DNA抽出法の確立 (2008)
  • Author(s): 上野真理子,菊池真美,森田英利,服部正平, 他
  • Organizer: 第2回日本ゲノム微生物学会
  • Place of Presentation: 大阪
• [Presentation] 腸内細菌叢のメタゲノム解析における細菌DNA抽出法の確立 (2008)
  • Author(s): 上野真理子, 菊池真美, 森田英利, 服部正平, 他
  • Organizer: 第2回 日本ゲノム微生物学会
  • Place of Presentation: 大阪
• [Book] メタゲノム解析技術の最前線(213) (2010)
  • Author(s): 林潤一郎、近藤伸二、森田英利、小島俊男、野口俊英
  • Publisher: シーエムシー出版
• [Book] メタゲノム解析技術の最前線 (2010)
  • Author(s): 林潤一郎
  • Publisher: シーエムシー出版
• [Remarks] ホームページ等