| Project/Area Number |
20300111
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
INAGAKI Naoyuki Nara Institute of Science and Technology, バイオサイエンス研究科, 准教授 (20223216)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMADA Tadayuki 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (80379552)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2010: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2009: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2008: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
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| Keywords | 神経細胞 / 極性 / 軸索 / Shootin1 / アクチン / 成長円錐 / クラッチ分子 / 樹状突起 / Netrin-1 / L1 |
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| Research Abstract |
In this study, we analyzed the mechanism for shootin1 accumulation in axons, and found that shootin1 is transported to the axonal growth cones by interacting dynamically the actin filaments in the "wave" structures. Accumulation of shootin1 in the growth cones promoted neurite outgrowth by mediating the linkage between actin filaments and L1-CAM, as a "clutch" molecule. Furthermore, to analyze the functions of shootin1 in the brain, we produced shootin1 knockout mice ; they showed agenesis in multiple regions in the brain, including the cerebral cortex, hippocampus, septum, olfactory bulb, corpus callosum, and hippocampal commissure. These results suggest that shootin1 plays an important role in neuronal polarization and brain development.
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