Studies of formation and function of neuronal circuits using alpha-chimerin mutant mice

• Project/Area Number: 20300118
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: National Institute of Genetics
• Principal Investigator: IWASATO Takuji 国立遺伝学研究所, 個体遺伝研究系, 教授 (00311332)
• Co-Investigator(Renkei-kenkyūsha): ITOHARA Shigeyoshi 独立行政法人理化学研究所, 行動遺伝学技術開発チーム, シニアチームリーダー (60252524) ; KATOH Hironori 京都大学, 生命科学研究科, 准教授 (50303847) ; NISHIMARU Hiroshi 筑波大学, 大学院・人間総合科学研究科, 准教授 (20302408)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000); Fiscal Year 2010: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2008: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
• Keywords: 神経科学 / マウス / 遺伝学 / 神経回路 / 発達

Research Abstract

We aim to reveal roles of alpha-chimerin Rac-GTPase activating protein(GAP) in formation and function of neuronal circuit in the mammalian brain. We generated global and Cre-mediated conditional knockout mice of alpha-chimerin. Furthermore, we generated knockout mouse lines specific to alpha1 and alpha2 isoforms. By analyzing these mice behaviorally and histologically, we found important roles of alpha-chimerin in hippocampus function.

Research Products

• [Journal Article] Role of adenylate cyclase 1 in retinofugal map development (2011)
  • Author(s): Dande, OS., Bhatt, S., Anishchenko, A, Elstrott, J., Iwasato, T., Swindell, EC, Xu, HP., Jamrich, M., Itohara, S., Feller, MB, Crair, MC.
  • Journal Title: J. Comp. Neurol Volume: (Epub ahead) Issue: 7 Pages: 1562-83
  • DOI: 10.1002/cne.23000
  • Peer Reviewed
• [Journal Article] Molecular characterization and gene disruption of a novel zinc-finger protein, HIT-4, expressed in rodent brain (2010)
  • Author(s): Tanabe, Y., Hirano, A., Iwasato, T., Itohara, S., et al.
  • Journal Title: J. Neurochem Volume: 112巻 Pages: 1035-1044
  • Peer Reviewed
• [Journal Article] Molecular characterization and gene disruption of a novelzinc-finger protein, HIT-4, expressed in rodent brain (2010)
  • Author(s): Tanabe, Y., et al.
  • Journal Title: J.Neurochem. 112 Pages: 1035-1044
  • Peer Reviewed
• [Journal Article] 中枢神経回路の活動依存的精緻化-マウス体性感覚野に「バレル」が形成される仕組み (2009)
  • Author(s): 岩里琢治
  • Journal Title: 生物の科学「遺伝」 Volume: 63巻 Pages: 79-85
• [Journal Article] Altered mnemonic functions and resistance to N-METHYL-d-Aspartate receptor antagonism by forebrain conditional knockout of glycine transporter (2009)
  • Author(s): Singer, P., Yee, BK., Feldon, J., Iwasato, T., Itohara, S., et al.
  • Journal Title: Neuroscience Volume: 161巻 Pages: 635-654
  • Peer Reviewed
• [Journal Article] Beta2-chimaerin binds to EphA receptors and regulates cell migration (2009)
  • Author(s): Takeuchi, S., Yamaki, N., Iwasato, T., Negishi, M., Katoh, H.
  • Journal Title: FEBS Lett Volume: 583巻 Pages: 1237-1242
  • Peer Reviewed
• [Journal Article] Altered mnemonic functions and resistance to N-METHYL-d-Aspartate receptor antagonism by forebrain conditional knockout of glycine transporter 1 (2009)
  • Author(s): Singer, P., et al.
  • Journal Title: Neuroscience 161 Pages: 635-654
  • Peer Reviewed
• [Journal Article] Beta2-chimaerin binds to EphA receptors and regulates cell migration (2009)
  • Author(s): Takeuchi, S., et al.
  • Journal Title: FEBS Lett. 583 Pages: 1237-1242
  • Peer Reviewed
• [Journal Article] 中枢神経回路の活動依存的精緻化-マウス体性感覚野に「バレル」が形成される仕組み (2009)
  • Author(s): 岩里琢治
  • Journal Title: 生物の科学 遺伝 63 Pages: 79-85
• [Journal Article] Altered mnemonic functions and resistance to N-METHYL-d-Aspartate receptor antagonism by forebrain conditional knockout of glycine transporter 1. (2009)
  • Author(s): Singer, P., et al.
  • Journal Title: Neuroscience (印刷中)
  • Peer Reviewed
• [Journal Article] Beta2-chiniaerin binds to EphA receptors and regulates cell migration. (2009)
  • Author(s): Takeuchi, S., et al.
  • Journal Title: FEBS Lett 583 Pages: 1237-42
  • Peer Reviewed
• [Journal Article] Cortical adenylyl cyclase 1 is required for thalamocortical synapse maturation and aspects of layerIVbarrel development (2008)
  • Author(s): Iwasato, T., Inan, M., Kanki, H., Erzurumlu, RS., Itohara, S., et al.
  • Journal Title: J. Neurosci Volume: 28巻 Pages: 5931-5943
  • Peer Reviewed
• [Journal Article] BMP signaling through BMPRIA in astrocytes is essential for proper cerebral angiogenesis and formation of the blood-brain-barrier (2008)
  • Author(s): Araya, R., Kubo, M., Ishii, K., Hashikawa, T., Iwasato, T., Itohara, S., Terasaki, T., Oohira, A., Mishina, Y., Yamada, M.
  • Journal Title: Mol. Cell Neurosci Volume: 38巻 Pages: 417-430
  • Peer Reviewed
• [Journal Article] Cortical adenylyl cyclase 1 is required for thalamocortical synapse maturation and aspects of layer IV barrel development. (2008)
  • Author(s): Iwasato, T., et al.
  • Journal Title: J Neurosci 28 Pages: 5931-43
  • Peer Reviewed
• [Journal Article] BMP signaling through BMPRIA in astrocytes is essential for proper cerebral angiogenesis and formation of the blood-brain-barrier. (2008)
  • Author(s): Araya, R., et al.
  • Journal Title: Mol Cell Neurosci 38 Pages: 417-30
  • Peer Reviewed
• [Presentation] Loss of Rac-GAP a-chimerin leads to alterations of emotional and cognitive behaviors in mice (2011)
  • Author(s): Iwata, R., Goto, H., Tanaka, M., Itohara, S., Iwasato, T.
  • Organizer: Neuroscience 2011
  • Place of Presentation: Washington, DC
• [Presentation] Loss of Rac-GAP a-chimerin leads to alterations of emotional and cognitive behaviors in mice (2011)
  • Author(s): Iwata, R., Goto, H., Tanaka, M., Itohara, S., Iwasato, T.
  • Organizer: Molecular and cellular cognition society
  • Place of Presentation: Washington, DC
• [Presentation] Rac GAP a-キメリン欠損マウスの網羅的行動解析Comprehensive behavioral analysis of Rac-GAP a-chimaerin deficient mice (2011)
  • Author(s): 岩田亮平.後藤大道.田中三佳.糸原重美
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 神奈川県横浜市
• [Presentation] Circuit refinement during postnatal development of the mouse somatosensory cortex (2010)
  • Author(s): 岩里琢治
  • Organizer: BMB2010第33回日本分子生物学会年会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-12-07
• [Presentation] Circuit Refinement during Postnatal Development of the Mouse Somatosensory Cortex. (2010)
  • Author(s): 岩里琢治
  • Organizer: BMB2010(第33回日本分子生物学会年会)シンポジウム発表
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2010-12-07
• [Presentation] Comprehensive behavioral analysis of miffy mice, a spontaneous mutant of Rac-GAPα-chimerin (2010)
  • Author(s): Iwata, R., Goto, H., Tanaka, M., Itohara, S., Iwasato, T.
  • Organizer: Molecular and Cellular Cognition Society-Society for Neuroscience Satellite Symposium 2010
  • Place of Presentation: San Diego Convention Center(アメリカ合衆国サンディエゴ)
  • Year and Date: 2010-11-11
• [Presentation] Comprehensive behavioral analysis of miffy mice, a spontaneous mutant of Rac-GAP α-chimerin (2010)
  • Author(s): Iwata, R., et al.
  • Organizer: Molecular and Cellular Cognition Society-Society for Neuroscience Satellite Symposium 2010
  • Place of Presentation: アメリカ合衆国・サンディエゴ
  • Year and Date: 2010-11-11
• [Presentation] マウス体性感覚系にみる神経回路の生後発達機構 (2009)
  • Author(s): 岩里琢治
  • Organizer: 頭部形成研究会
  • Place of Presentation: 静岡県伊豆市
  • Year and Date: 2009-11-16
• [Presentation] αキメリンによる運動制御の神経回路形成 (2009)
  • Author(s): 岩里琢治
  • Organizer: 2008年度国立遺伝学研究所研究会「行動遺伝学研究会」(シンポジウム, 招待講演)
  • Place of Presentation: 三島
  • Year and Date: 2009-03-12
• [Presentation] 左右の手足の非対象な動きを可能にする神経回路形成の分子機構 (2008)
  • Author(s): 岩里琢治
  • Organizer: BMB2008第31回日本分子生物学会年会
  • Place of Presentation: 兵庫県神戸市
  • Year and Date: 2008-12-12
• [Presentation] 左右の手足の非対称な動きを可能にする神経回路形成の分子機構 (2008)
  • Author(s): 岩里琢治
  • Organizer: 分子生物学会(シンポジウム)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] マウス体性感覚系発達の遺伝学的解析 (2008)
  • Author(s): 岩里琢治
  • Organizer: 認識と形成研究会(シンポジウム)
  • Place of Presentation: 神戸
  • Year and Date: 2008-11-01
• [Presentation] Dissociation of pre-and postsynaptic mechanisms in maturation of the barrel cortex (2008)
  • Author(s): Iwasato, T.
  • Organizer: The 6th FENS forum of European Neuroscience
  • Place of Presentation: Geneva
  • Year and Date: 2008-07-15
• [Presentation] Dissociation of Pre-and Postsynaptic Mechanisms in Maturation of the Barrel Cortex (2008)
  • Author(s): Takuii Iwasato
  • Organizer: The 6^<th> FENS forum of European Neuroscience(シンポジウム, 招待講演)
  • Place of Presentation: Geneva
  • Year and Date: 2008-07-15
• [Presentation] Dissociation of pre-and postsynaptic mechanisms of barrel cortex development (2008)
  • Author(s): 岩里琢治
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-10
• [Presentation] Dissociation of pre- and postsynaptic mechanisms of barrelcortex development (2008)
  • Author(s): Takuji Iwasato
  • Organizer: 神経科学学会(ワークショップ, 招待講演)
  • Place of Presentation: 東京
  • Year and Date: 2008-07-10
• [Presentation] 哺乳類中枢神経回路発達の遺伝学 (2008)
  • Author(s): 岩里琢治
  • Organizer: 筑波大学若手イニシアティブセミナーシリーズ(セミナー)
  • Place of Presentation: 筑波
  • Year and Date: 2008-06-13
• [Presentation] 運動系神経回路形成におけるephrin-Ephシグナル伝達機構 (2008)
  • Author(s): 岩里琢治
  • Organizer: 神経組織の成長・再生・移植研究会第23回学術集会(基調講演, 招待講演)
  • Place of Presentation: 千葉
  • Year and Date: 2008-05-17
• [Presentation] 哺乳類中枢神経回路発達の統合的理解-マウス体性感覚系を主要なモデルとして- (2008)
  • Author(s): 岩里琢治
  • Organizer: 生理研セミナー(セミナー)
  • Place of Presentation: 岡崎
  • Year and Date: 2008-04-25
• [Presentation] 新規突然変異マウス(ミッフィー変異マウス)が明らかにしたEphrin/Ephシグナル伝達機構 (2008)
  • Author(s): 岩里琢治
  • Organizer: 基礎生物学研究所研究会 : リン酸化シグナルの統合的理解を目指して(シンポジウム, 招待講演)
  • Place of Presentation: 岡崎
  • Year and Date: 2008-04-19
• [Book] Miffy変異マウス:ウサギのように両足をそろえて歩く自然発生変異マウス(分担執筆)<seriesモデル動物利用マニュアル>疾患モデルの作製と利用-脳・神経疾患(三品昌美編) (2011)
  • Author(s): 岩里琢治,糸原重美
• [Remarks]
  • URL: http://www.nig.ac.jp/section/iwasato/iwasato-j.html
• [Remarks]
  • URL: http://homepage3.nifty.com/iwasato/
• [Remarks]
  • URL: http://homepage3.nifty.com/iwasato/
• [Remarks]
  • URL: http://homepage3.nifty.com/iwasato/