Research on the intermediate progenitors of GABAergic neurons found in the subventricular zone of the mouse neocortex
| Project/Area Number |
20300121
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Kumamoto University |
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Principal Investigator |
TAMAMAKI Nobuaki 熊本大学, 大学院・生命科学研究部, 教授 (20155253)
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| Co-Investigator(Renkei-kenkyūsha) |
WATANABE Keisuke 熊本大学, 大学院・生命科学研究部, 助教 (20446264)
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| Project Period (FY) |
2008 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2009: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2008: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
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| Keywords | 再生医学 / 神経科学 / 大脳皮質 / 神経前駆細胞 / 神経細胞 / intermediate progenitor / GABA / neocortex / proliferation / array analysis |
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| Research Abstract |
In the embryonic neocortex, neuronal precursors are generated in the ventricular zone(VZ) and accumulate in the cortical plate. Recently, the subventricular zone(SVZ) of the embryonic neocortex was recognized as an additional neurogenic site for both principal excitatory neurons and GABAergic inhibitory neurons. To gain insight into the neurogenesis of GABAergic neurons in the SVZ, we investigated the characteristics of intermediate progenitors of GABAergic neurons(IPGNs) in mouse neocortex by immunohistochemistry, immunocytochemistry, single-cell RT-PCR, and single-cell array analysis. IPGNs were identified by their expression of some neuronal and cell cycle markers. Moreover, we investigated the origins of the neocortical IPGNs by Cre-loxP fate mapping in transgenic mice and the transduction of part of the telencephalic VZ by Cre-reporter plasmids, and found them in the medial and lateral ganglionic eminence. Therefore they must migrate tangentially within the telencephalon to reach the neocortex. Cell-lineage analysis by simple-retrovirus transduction revealed that the neocortical IPGNs self-renew and give rise to a small number of neocortical GABAergic neurons and a large number of granule and periglomerular cells in the olfactory bulb. IPGNs are maintained in the neocortex and may act as progenitors for adult neurogenesis.
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] Tangential migration and proliferation of intermediate progenitors of GABAergic neurons in the mouse telencephalon2011
Author(s)
Wu S, Esumi S, Watanabe K, Chen J, Nakamura KC, Nakamura K, Kometani K, Minato N, Yanagawa Y, Akashi K, Sakimura K, Kaneko T, Tamamaki N
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Journal Title
Development
Volume: 138
Pages: 2499-2509
Related Report
Peer Reviewed
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[Journal Article] Cortical GABAergic Neurons : Stretching it Remarks, Main Conclusions and Discussion2010
Author(s)
Clancy B, Defelipe J, Espinosa A, Faire' n A, Jinno S, Kanold P, Luhmann HJ, Rockland KS, Tamamaki N, Yan XX.
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Journal Title
Front Neuroanat
Volume: 4
Pages: 7-7
Related Report
Peer Reviewed
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[Journal Article] Cortical GABAergic Neurons : Stretching it Remarks, Main Conclusions and Discussion2010
Author(s)
Clancy B, Defelipe J, Espinosa A, Fairen A, Jinno S, Kanold P, Luhmann HJ, Rockland KS, Tamamaki N, Yan XX
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Journal Title
Front Neuroanat
Volume: 4
Pages: 7-7
Related Report
Peer Reviewed
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[Journal Article] Noradrenergic excitation of a subpopulation of GABAergic cells in the basolateral amygdala via both activation of nonselective cationic conductance and supression of resting K+conductance : A study using GAD67-GFP knock-in mice2008
Author(s)
Kaneko K, Tamamaki N, Owada H, Kakizaki T, Kume N, Totsuka M, Yamamoto T, Yawo H, Yagi T, Obata K, Yanagawa Y.
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Journal Title
Neuroscience
Volume: 157
Pages: 781-97
Related Report
Peer Reviewed
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