Regulatory mechanism and cellular dynamics of exocytosis of BDNF-containing secretory vesicles by CAPS2
| Project/Area Number |
20300134
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
FURUICHI Tei-Ichi The Institute of Physical and Chemical Research, 分子神経形成研究チーム, チームリーダー (50219094)
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| Co-Investigator(Renkei-kenkyūsha) |
SADAKATA Tetsushi 独立行政法人理化学研究所, 分子神経形成研究チーム, 基礎科学特別研究員 (90391961)
SHINODA Yo 独立行政法人理化学研究所, 分子神経形成研究チーム, 研究員 (80403096)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2010: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2009: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2008: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Keywords | CAPS2 / 脳由来神経栄養因子 / 有芯小胞 / トランスゴルジ輸送 / 不安行動 / GABA / 抑制性シナプス / 脳由来神経脳栄養因子 / GABA作動性 / small GTPase Arf4/5 / トランスゴルジ網trans-Golgi / BDNF / 分泌 / 長期増強 / 海馬シナプス / 自閉症 / 発達障害 / 有芯性分泌小胞 |
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| Research Abstract |
We analyzed cellular function and biological significance of secretion-associated protein CAPS and reported the following findings.
1) CAPS2 promotes kinetics of activity-dependent secretion of brain-derived neuotrophic factor BDNF. CAPS2 KO mice have impairments in development and function of GABAergic inhibitory circuits and exhibit increased anxiety-like phenotype.
2) In addition to involvement in exocytosis of dense core vesicles, CAPS associates with small GTPase Arf4/5 and is associated with biogenesis and trafficking of dense-core vesicles in the trans-Golgi network.
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Report
(4 results)
Research Products
(93 results)
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[Journal Article] Systematizing and cloning of genes involved in the cerebellar cortex circuit development.2011
Author(s)
Furuichi T., Shiraishi-Yamaguchi Y., Sato A., Sadakata T., Huang J., Shinoda Y., Hayashi K., Mishima Y., Tomomura M., Nishibe H., Yoshikawa F.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Phospholipase D family member 4, a transmembrane glycoprotein with no phospholipase D activity, expression in spleen and early postnatal microglia.2010
Author(s)
Yoshikawa F., Banno Y., Otani Y., Yamaguchi Y., Nagakura-Takagi Y., Morita N., Sato Y., Saruta C., Nishibe H., Sadakata T., Shinoda Y., Hayashi K., Mishima Y., Baba H., Furuichi T.
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Journal Title
NAID
Related Report
Peer Reviewed
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[Journal Article] Expression of the IP3R1 promoter-driven nls-lacZ transgene in Purkinje cell parasagittal arrays of developing mouse cerebellum.2010
Author(s)
Furutama D., Morita N., Takano R., Sekine Y., Sadakata T., Shinoda Y., Hayashi K., Mishima Y., Mikoshiba K., Hawkes R., Furuichi T.
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Journal Title
J.Neurosci.Res 88
Pages: 2810-2825
Related Report
Peer Reviewed
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[Journal Article] Opalin, a transmembrane sialylglycoprotein located in the CNS myelin paranodal loop membrane.2008
Author(s)
Yoshikawa F., Sato Y., Tohyama K., Akagi T., Hashikawa T., Nagakura-Takagi Y., Sekine Y., Morita N., Baba H., Suzuki Y., Sugano S., Sato A., Furuichi T.
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Journal Title
J.Biol.Chem 283
Pages: 20830-20840
Related Report
Peer Reviewed
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[Journal Article] Phase advance of the light-dark cycle perturbs diurnal rhythms of brain-derived neurotrophic factor and neurotrophin-3 levels, which reduces synaptophysin-positive presynaptic terminals in the cortex of juvenile rats.
Author(s)
Hamatake M., Miyazaki N., Sudo K., Matsuda M., Sadakata T., Furuya A., Ichisaka S., Hata Y., Nakagawa C., Nagata K., Furuichi T., Katoh-Semba R.
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Journal Title
Related Report
Peer Reviewed
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