Organization and basic functional mechanism of cerebellar modules

• Project/Area Number: 20300137
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: SUGIHARA Izumi Tokyo Medical and Dental University, 大学院・医歯学総合研究科, 教授 (60187656)
• Research Collaborator: 藤田 啓史 東京医科歯科大学, 大学院・医歯学総合研究科, 大学院博士課程学生
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000); Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2009: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2008: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
• Keywords: 小脳 / 後索核 / プルキンエ細胞 / アルドラーゼC / マーモセット / 苔状線維 / ラット / 神経解剖学 / 小脳皮質 / 体性感覚 / 小脳核 / 霊長類 / 下オリーブ核 / ビオチン化デキストラン

Research Abstract

The cerebellar module is organized by the molecular expression pattern and input-output axonal connections of Purkinje cells, climbing fibers and mossy fibers. We related axonal connections of Purkinje cells and mossy fiber axons that originate from the dorsal column nuclei to the aldolase C expression pattern. Combining the results with our previous findings on the climbing fiber projection pattern, we clarified the organization of cerebellar modules, and show how cerebellar input and output functions are correlated topographically. Furthermore, we have shown similarity of the cerebellar modular organization between rodents and primates by analyzing the marmoset cerebellum in addition to the rat cerebellum.

Research Products

• [Journal Article] Projection patterns of single mossy fiber axons originating from the dorsal column nuclei mapped on the aldolase C compartments in the rat cerebellar cortex. (2011)
  • Author(s): Quy, P.N., Fujita, H., Sakamoto, Y., Na, J., Sugihara I.
  • Journal Title: J.Comp.Neurol. 519 Pages: 874-899
• [Journal Article] Close correlation between the birthdate of Purkinje cells and the longitudinal compartmentalization of the mouse adult cerebellum. (2011)
  • Author(s): Namba K, Sugihara I, Hashimoto M.
  • Journal Title: J.Comp.Neurol. in press
• [Journal Article] Compartmentalization of the deep cerebellar nuclei based on afferent projections and aldolase C expression. (2011)
  • Author(s): 杉原泉
  • Journal Title: Cerebellum in press
• [Journal Article] The DIADEM data sets : Representative light microscopy images of neuronal Morphology to advance automation of digital reconstructions. (2011)
  • Author(s): Brown KM, Barrionuevo G, Canty AJ, De Paola V, Hirsch JA, Jefferis GS, Lu J, Snippe M, Sugihara I, Ascoli GA.
  • Journal Title: Neuroinformatics in press
• [Journal Article] Bright field neuronal preparation optimized for automatic computerized reconstruction, a case with cerebellar climbing fibers. (2011)
  • Author(s): 杉原泉
  • Journal Title: Neuroinformatics in press
• [Journal Article] 小脳の入出力神経回路と分子発現 (2011)
  • Author(s): 杉原泉
  • Journal Title: Medical Science Digest 37 Pages: 126-127
• [Journal Article] Projection patterns of single mossy fiber axons originating from the dorsal column nuclei mapped on the aldolase C compartments in the rat cerebellar cortex. (2011)
  • Author(s): Quy, P.N., Fujita, H., Sakamoto, Y., Na, J., Sugihara, I.
  • Journal Title: J.Comp.Neurol. Volume: 519 Pages: 874-899
  • Peer Reviewed
• [Journal Article] 小脳の入出力神経回路と分子発現 (2011)
  • Author(s): 杉原泉
  • Journal Title: Medical Science Digest Volume: 37 Pages: 126-127
• [Journal Article] Organization of the marmoset cerebellum in three-dimensional space : lobulation, aldolase C compartmentalization and axonal projection. (2010)
  • Author(s): Fujita, H., Oh-Nishi, A., Obayashi, S., Sugihara I.
  • Journal Title: J.Comp.Neurol. 518 Pages: 1764-1791
• [Journal Article] Maternal immune activation by polyriboinosinic-polyribocytidilic acid injection produces synaptic dysfunction but not neuronal loss in the hippocampus of juvenilerat offspring. (2010)
  • Author(s): Oh-Nishi A, Obayashi S, Sugihara I, Minamimoto T, Suhara T.
  • Journal Title: Brain Res. Volume: 1363 Pages: 170-179
  • Peer Reviewed
• [Journal Article] Organization of the marmoset cerebellum in three-dimensional space : lobulation, aldolase C compartmentalization and axonal projection. (2010)
  • Author(s): Fujita H.
  • Journal Title: J.Comp.Neurol. 518 Pages: 1764-1791
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): 杉原泉
  • Journal Title: 小脳皮質の部位局在性.Annual Review 2010神経(鈴木則宏、祖父江元、荒木信夫、宇川義一、川原信隆編)(中外医学社) Pages: 9-16
• [Journal Article] Projection of reconstructed single Purkinje cell axons in relation to the cortical and nuclear aldolase C compartments of the rat cerebellum. (2009)
  • Author(s): Sugihara, I., Fujita, H., Na, J., Quy, P.N., Li, B.Y., Ikeda, D.
  • Journal Title: J.Comp.Neurol. 512 Pages: 282-304
• [Journal Article] 小脳の局在-その構造的基盤 (2009)
  • Author(s): 杉原泉
  • Journal Title: Clinical Neuroscience 27 Pages: 765-768
• [Journal Article] 小脳の局在-その構造的基盤. (2009)
  • Author(s): 杉原泉
  • Journal Title: Clinical Neuroscience 27 Pages: 765-768
• [Journal Article] Projection of reconstructed single Purkinje cell axons in relation to the cortical and nuclear aldolase C comp artments of the rat cerebellum. (2009)
  • Author(s): Sugihara I.
  • Journal Title: J. Comp. Neurol. 512 Pages: 282-304
  • Peer Reviewed
• [Journal Article] BCL-2 counteracts Doppel-induced apoptosis of prion-protein-deficient Purkinje cells in the Ngsk Prnp^<0/0> mouse. (2008)
  • Author(s): Heitz, S., Gautheron, V., Lutz, Y., Rodeau, J.L., Zanjani, H.S., Sugihara, I., Bombarde, G., Richard, F., Fuchs, J.P., Vogel, M.W., Mariani, J., Bailly, Y.
  • Journal Title: Dev.Neurobiol. 68 Pages: 332-348
• [Presentation] 入出力神経ネットワークに基づく小脳の機能構築 (2011)
  • Author(s): 杉原泉
  • Organizer: 平成22年度 生理学研究所研究会「行動制御における脳領域間の機能連関」
  • Place of Presentation: 愛知県岡崎市生理学研究所
  • Year and Date: 2011-01-07
• [Presentation] 入出力神経ネットワークに基づく小脳の機能構築. (2011)
  • Author(s): 杉原泉
  • Organizer: 生理学研究所研究会「行動綱御における脳領域間の機能連関」
  • Place of Presentation: 愛知県岡崎市生理学研究所
  • Year and Date: 2011-01-07
• [Presentation] Longitudinal compartmentalization in the marmoset cerebellum. (2010)
  • Author(s): Fujita, H., Sugihara I.
  • Organizer: Program No.785.16. Neuroscience Meeting Planner. San Diego, CA : Society for Neuroscience, 2010. Online. (The 40th annual meeting of the Society for Neuroscience
  • Place of Presentation: San Diego
  • Year and Date: 2010-11-17
• [Presentation] Longitudinal compartmentalization in the marmoset cerebellum. (2010)
  • Author(s): Fujita, H., Sugihara, I.
  • Organizer: The 40th annual meeting of the Society for Neuroscience
  • Place of Presentation: San Diego, CA, U.S.A.
  • Year and Date: 2010-11-17
• [Presentation] 小脳の機能、眼球運動に 対する小脳の関わり (2010)
  • Author(s): 杉原泉
  • Organizer: 第32回日本めまい平衡機能検査技術者会における
  • Place of Presentation: 東京、千代田区、学術総合センター 招待講演
  • Year and Date: 2010-10-02
• [Presentation] 小脳の機能、眼球運動に対する小脳の関わり (2010)
  • Author(s): 杉原泉
  • Organizer: 第32回日本めまい平衡機能検査技術者会
  • Place of Presentation: 東京、千代田区学衛総合センター
  • Year and Date: 2010-10-02
• [Presentation] Significance and origin of the longitudinal stripe organization in the cerebellar cortex. (2010)
  • Author(s): 杉原泉
  • Organizer: Neurosci.Res., page e38. 68, Suppl 1(The 33rd Annual Meeting of the Japan Neuroscience Society)
  • Place of Presentation: Kobe
  • Year and Date: 2010-09-04
• [Presentation] Significance and origin of the longitudinal stripe organization in the cerebellar cortex. (2010)
  • Author(s): Sugihara, I.
  • Organizer: IADEM conference
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2010-09-04
• [Presentation] Development of cerebellar parasagittal compartmentalization studied in a transgenic mouse strain carrying the LacZ composite gene. (2010)
  • Author(s): Fujita, H., Furuichi, T., Sugihara I.
  • Organizer: Neurosci.Res., page e130. 68, Suppl 1(The 33rd Annual Meeting of the Japan Neuroscience Society)
  • Place of Presentation: Kobe
  • Year and Date: 2010-09-02
• [Presentation] T.Development of cerebellar parasagittal compartmentalization studied in a transgenic mouse strain carrying the LacZ Composite gene. (2010)
  • Author(s): Fujita, H., Furuichi, Sugihara, I.
  • Organizer: The 33rd Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2010-09-02
• [Presentation] Formation and malformation of climbing fibers : Single axon morphology tells the difference. (2010)
  • Author(s): 杉原泉
  • Organizer: Invited Lecture in DIADEM conference, HHMI Janelia Research Campus
  • Place of Presentation: Ashburn, VA, U.S.A.
  • Year and Date: 2010-08-30
• [Presentation] Formationtells and malformation of climbing fibers :Single axon morphology the difference. (2010)
  • Author(s): Sugihara, I.
  • Organizer: DIADEM conference
  • Place of Presentation: HHMI Janelia Research Campus Ashburn, VA, U.S.A.
  • Year and Date: 2010-08-30
• [Presentation] 小脳の軸索投射と縦縞構造 (2010)
  • Author(s): 杉原泉
  • Organizer: CBIR若手シンポジウム
  • Year and Date: 2010-02-01
• [Presentation] Subzonal organization of olivo-cortico-nuclear circuits in the rat. (2009)
  • Author(s): Sugihara I., Cerminara, N., Apps, R.
  • Organizer: (Second International Congress of the Society for Research on the Cerebellum)
  • Place of Presentation: Chicago,
  • Year and Date: 2009-10-16
• [Presentation] Subzonal organization of olivo-cortico-nuclear circuits in the rat. (2009)
  • Author(s): Sugihara I.
  • Organizer: Second International Congress of the Society for Research on the Cerebellum
  • Place of Presentation: Chicago
  • Year and Date: 2009-10-16
• [Presentation] Development and differentiation of the cerebellum: normal and abnormal morphogenesis of climbing fibers. (2009)
  • Author(s): 杉原泉
  • Organizer: Neurosci.Res., 65, Suppl 1, S33.(The 32nd Annual Meeting of the Japan Neuroscience Society)
  • Place of Presentation: Nagoya
  • Year and Date: 2009-09-18
• [Presentation] Development and differentiation of the cerebellum : normal and abnormal morphogenesis of climbing fibers. (2009)
  • Author(s): Sugihara I.
  • Organizer: The 32nd Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-18
• [Presentation] Longitudinal compartmental organization of the primate cerebellum. (2009)
  • Author(s): Fujita H.
  • Organizer: The 32nd Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-18
• [Presentation] Influence of maternal immune activation in embryonic stage on hippocampal function in juvenile stage. (2009)
  • Author(s): Oh-Nishi A.
  • Organizer: The 32nd Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-18
• [Presentation] Mediolateral compartments determined by the birthdate of Purkinje cells correlates closely with Zebrin II-pattern in adult cerebellum. (2009)
  • Author(s): Hashimoto M.
  • Organizer: The 32nd Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-17
• [Presentation] Anatomical basis for cerebellar functional localization : lobules, stripes, micromodules and axonal connections. (2009)
  • Author(s): Sugihara I.
  • Organizer: 36th International Congress of Physiological Sciences
  • Place of Presentation: 京都
  • Year and Date: 2009-07-31
• [Presentation] Anatomical basis for cerebellar functional localization : lobules, stripes, micromodules and axonal connections. (2009)
  • Author(s): Sugihara I, Hirofumi Fujita, Pham Nguyen Quy, Asuka Maki
  • Organizer: 36th International Congress of Physiological Sciences (IUPS 2009)
  • Place of Presentation: Kyoto, Japan(Poster presentation on July 31)
• [Presentation] Morphological diversity and spatial orientation of rat cerebellar climbing fibers : branch types and cortical regions. (2008)
  • Author(s): Brown, K. M.
  • Organizer: Neuroscience 2008, the 38th annual meeting of the Society for Neuroscience
  • Place of Presentation: Washington, DC
  • Year and Date: 2008-11-19
• [Presentation] Cerebellar localization of somatosensory submodarities : Projection pattern of individual mossy fibers from the dorsal columnnuclei in the rat. (2008)
  • Author(s): Quy, P. N.
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-10
• [Presentation] Correlation between the mediolateral compartments formed by birthdate-related Purkinje cells and zebrin II-bands. (2008)
  • Author(s): Hashimoto, M.
  • Organizer: 第31回日本神経科学大会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-10
• [Presentation] 小脳の構築と機能 (2008)
  • Author(s): 杉原 泉
  • Organizer: 第2 3回日本大脳基底核研究
  • Place of Presentation: 山梨県南都留郡富士河口湖町
  • Year and Date: 2008-07-06
• [Presentation] Functional significance of aldolase C expression pattern in the cerebellar cortex and cerebellar nuclei. (2008)
  • Author(s): Sugihara I., Shinoda, Y.
  • Organizer: Neural Mechanisms in Control of Eye, Head andLimb Movements, A Satellite Symposium to the 25th Barany Society Meeting, Abstract p29
  • Place of Presentation: Ohtsu
  • Year and Date: 2008-03-30
• [Presentation] 小脳の構築と機能 (2008)
  • Author(s): 杉原泉
  • Organizer: 第23回日本大脳基底核研究
  • Place of Presentation: 精進マウントホテル、〒401-0336山梨県南都留郡富士河口湖町精進301、TEL 0555-87-2200 FAX 0555-87-2122)招待講演
• [Book] A.Mendez-Vilas and J.Diaz eds.Microscopy : Science, Technology, Applications and Education, Microscopy Book Series(A computer-aided light microscopy system for three-dimensional reconstruction of axonal projections. No.4.) (2011)
  • Author(s): Sugihara, I., Fujita, H.
  • Publisher: Badajoz : Formatex.
• [Book] Annual Review 2010神経(小脳皮質の部位局在性)(鈴木則宏、祖父江元、荒木信夫、宇川義一、川原信隆編) (2010)
  • Author(s): 杉原泉
  • Publisher: 中外医学社
• [Book] Microscopy : Science, Technology, Applications and Education, Microscopy Book Series No.4.(A computer-aided light microscopy system for three-dimensional reconstruction of axonal projections.)(A.Mendez-Vilas and J.Diaz eds.) (2010)
  • Author(s): Sugihara I., Fujita, H.
  • Publisher: Formatex
• [Remarks] ホームページ等
  • URL: http://www.tmd.ac.jp/med/phy1/phy1.html
• [Remarks]
  • URL: http://www.tmd.ac.jp/med/phyl/phyl.html
• [Remarks]
  • URL: http://www.tmd.ac.jp/med/phy1/phy1.html