Development of the antigen detection-based diagnosis method specific for murine pathogenic Helicobacter species

• Project/Area Number: 20300143
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: University of Tsukuba
• Principal Investigator: KUNITA Satoshi University of Tsukuba, 大学院・人間総合科学研究科, 講師 (10195472)
• Co-Investigator(Kenkyū-buntansha): YAGAMI KenーIchi 筑波大学, 大学院・人間総合科学研究科, 教授 (40166476) ; GOTO Kazuo (財)実験動物中央研究所, ICLASモニタリングセンター, 室長 (00205593) ; HAYASHIMOTO Nobuhito (財)実験動物中央研究所, ICLASモニタリングセンター, 研究員 (30332208)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000); Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2009: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2008: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
• Keywords: 感染症 / 診断法 / マウス / ヘリコバクター / サンドイッチELISA法 / イムノクロマト法 / H.hepaticus / モノクローナル抗体 / サンドイッチELISA / イムノクロマト / 感染症診断

Research Abstract

To develop an antigen detection-based diagnosis method for murine pathogenic Helicobacter species, H. hepaticus, we prepared a monoclonal antibody (MAb#5-12D) specific for H.hepaticus. A sandwich ELISA using the MAb#5-12D detected as little as 104CFU of H. hepaticus. In addition, an immunochromatographic method allowed specific, quick and easy detection of H. hepaticus within 10 min.

Research Products

• [Journal Article] Simultaneous Detection of antibodies to mouse hepatitis virus recombinant structural proteins by a microsphere-based multiplex fluorescent immunoassay. (2011)
  • Author(s): S.Kunita, K Kato, M Ishida, K Hagiwara, S Kameda, T Ishida, A Takakura, K Goto, F Sugiyama, K Yagami
  • Journal Title: Clin Vaccine Immunol. 18 Pages: 758-766
  • Peer Reviewed
• [Journal Article] Identification and characterization of hemolysin-like proteins similar to RTX toxin in Pasteurella pneumotropica. (2009)
  • Author(s): H.Sasaki, E.Kawamoto, Y.Tanaka, T.Sawada, S.Kunita, K.Yagami
  • Journal Title: J.Bacteriol. 191 Pages: 3698-3705
  • Peer Reviewed
• [Journal Article] Comparative analysis of Pasteurella pneumotropica isolates from laboratory mice and rats (2009)
  • Author(s): H.Sasaki, E.Kawamoto, Y.Tanaka, T.Sawada, S.Kunita, K.Yagami
  • Journal Title: Antonie Van Leeuwenhoek 95 Pages: 311-317
  • Peer Reviewed
• [Presentation] マウス・ラット微生物検査の最近の話題 (2010)
  • Author(s): 國田智
  • Organizer: 第57回日本実験動物学会総会JALAMシンポジウム
  • Place of Presentation: 京都テルサ
  • Year and Date: 2010-05-11
• [Presentation] マウス・ラット微生物検査の最近の話題 (2010)
  • Author(s): 國田智
  • Organizer: 第57回日本実験動物学会総会JALAMシンポジウム
  • Place of Presentation: 京都テルサ(京都)
  • Year and Date: 2010-05-11
• [Presentation] Diagnosis method based on sandwich ELISA using Helicobacter hepaticus-specific monoclonal antibody (2009)
  • Author(s): 冨家彰浩, 國田智, 後藤一雄, 杉山文博, 八神健一
  • Organizer: 60th American Association for Laboratory Animal Science National Meeting
  • Place of Presentation: Denver Convention Center, Colorado, USA
  • Year and Date: 2009-11-09
• [Presentation] Helicobacter hepaticus特異的モノクローナル抗体を用いた抗原診断法の開発 (2009)
  • Author(s): 冨家彰浩, 國田智, 後藤一雄, 杉山文博, 八神健一
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: 大宮ソニックシティ
  • Year and Date: 2009-05-14
• [Presentation] Diagnosis method based on sandwich ELISA using Helicobacter hepaticus-specific monoclonal antibody (2009)
  • Author(s): Akihiro Fuke
  • Organizer: 60th American Association for Laboratory Animal Science National Meeting
  • Place of Presentation: Denver Convention Center, Colorado(USA)
• [Presentation] Helicobacter hepaticusウレアーゼBサブユニットに対するモノクローナル抗体の作製と診断への応用 (2008)
  • Author(s): 冨家彰浩, 國田智, 後藤一雄, 杉山文博, 八神健一
  • Organizer: 第55回日本実験動物学会総会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2008-05-16
• [Book] 実験動物としてのマウス・ラットの感染症対策と予防((社)日本実験動物学会監修、アドスリー) (2011)
  • Author(s): 國田智, 他
  • Total Pages: 176
• [Book] 実験動物としてのマウス・ラットの感染症対策と予防 (2011)
  • Author(s): (社)日本実験動物学会監修
  • Total Pages: 176
  • Publisher: アドスリー
• [Remarks] ホームページ等
  • URL: http://www.md.tsukuba.ac.jp/basic-med/yagami/helico.html
• [Remarks]
  • URL: http://www.md.tsukuba.ac.jp/basic-med/yagami/index.html