Development of the antigen detection-based diagnosis method specific for murine pathogenic Helicobacter species
| Project/Area Number |
20300143
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | University of Tsukuba |
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Principal Investigator |
KUNITA Satoshi University of Tsukuba, 大学院・人間総合科学研究科, 講師 (10195472)
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| Co-Investigator(Kenkyū-buntansha) |
YAGAMI KenーIchi 筑波大学, 大学院・人間総合科学研究科, 教授 (40166476)
GOTO Kazuo (財)実験動物中央研究所, ICLASモニタリングセンター, 室長 (00205593)
HAYASHIMOTO Nobuhito (財)実験動物中央研究所, ICLASモニタリングセンター, 研究員 (30332208)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2009: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2008: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Keywords | 感染症 / 診断法 / マウス / ヘリコバクター / サンドイッチELISA法 / イムノクロマト法 / H.hepaticus / モノクローナル抗体 / サンドイッチELISA / イムノクロマト / 感染症診断 |
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| Research Abstract |
To develop an antigen detection-based diagnosis method for murine pathogenic Helicobacter species, H. hepaticus, we prepared a monoclonal antibody (MAb#5-12D) specific for H.hepaticus. A sandwich ELISA using the MAb#5-12D detected as little as 104CFU of H. hepaticus. In addition, an immunochromatographic method allowed specific, quick and easy detection of H. hepaticus within 10 min.
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Report
(4 results)
Research Products
(13 results)
