| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2010: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2009: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2008: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
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| Research Abstract |
We investigated the expression pattern of the established EGTC mouse lines by X-gal staining. We have analyzed 84 mouse lines, and opened expression patterns of 67 clones. In many case, multiple organs showed strong expression of beta-geo protein. There are almost no relationship between X-gal staining results and TPM (transcripts per million) scores of the EST profile (Unigene). This finding shows the importance of actually monitoring the expression pattern of the reporter gene, when we consider it as promoter resources to produce transgenic mice.
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