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Development of Bioassay System with Microwell Slide Towards High throughput Single Cell Analysis

Research Project

Project/Area Number 20300149
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionHokkaido University

Principal Investigator

OHASHI Toshiro  Hokkaido University, 大学院・工学研究院, 教授 (30270812)

Co-Investigator(Kenkyū-buntansha) SATO Masaaki  東北大学, 大学院・医工学研究科, 教授 (30111371)
SAKAMOTO Naoya  東北大学, 大学院・工学研究科, 助教 (20361115)
MAEDA Eijiro  北海道大学, 大学院・工学研究院, 助教 (20581614)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2008: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Keywordsバイオメカニクス / 単一細胞診断 / マイクロウェル / マイクロフルイディクス / バイオチップ / バイオMEMS / マクロウェル / マクロフルディクス
Research Abstract

Since most laboratory bioassays used for cell analysis are designed to measure average effects on cell population, it is difficult to study characteristics of individual cells such as patient's tumor cell samples in a high throughput manner. Recently, a novel microwell slide that allows several thousands of single cells to be addressed to an exact position, cultured for weeks, and treated separately in high throughput has been developed. In this project we have proposed a bioassay system consisting of an active microfluidic device integrated on the microwell plate for improved control of, and new applications for the single cells.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (10 results)

All 2011 2010 2009 2008

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (7 results) Book (1 results)

  • [Journal Article] A microwell array device with integrated microfluidic components for enhanced single-cell analysis2009

    • Author(s)
      Sara Lindstrom, Kiichiro Mori, Toshiro Ohashi, Helene Andersson-Svahn
    • Journal Title

      Electrophoresis Vol.30

      Pages: 4166-4171

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A microwell array device with integrated microfluidic components for enhanced single-cell analysis2009

    • Author(s)
      Sara Lindstrom
    • Journal Title

      Electrophoresis 30

      Pages: 4166-4171

    • Related Report
      2009 Annual Research Report 2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 流れ負荷血管内皮細胞の牽引力計測デバイスの開発2011

    • Author(s)
      大橋俊朗
    • Organizer
      日本機械学会第23回バイオエンジニアリング講演会
    • Place of Presentation
      熊本大学 熊本
    • Year and Date
      2011-01-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Integrin Expression Modulates Smooth Muscle Cell Traction Forces2010

    • Author(s)
      Toshiro Ohashi
    • Organizer
      6th World Congress of Biomechanics
    • Place of Presentation
      Suntec Convention Centre Singapore
    • Year and Date
      2010-08-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] Novel bioassay systems for single cells2009

    • Author(s)
      Toshiro Ohashi
    • Organizer
      The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS2009)
    • Place of Presentation
      Jeju, Korea
    • Year and Date
      2009-11-03
    • Related Report
      2010 Final Research Report
  • [Presentation] Novel bioassay systems for single cells2009

    • Author(s)
      Toshiro Ohashi
    • Organizer
      The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences(μTAS2009)
    • Place of Presentation
      済州島,韓国
    • Year and Date
      2009-11-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] Novel bioassay systems for single cells2009

    • Author(s)
      Toshiro Ohashi
    • Organizer
      The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences(μTAS2009)
    • Place of Presentation
      済州島, 韓国
    • Year and Date
      2009-11-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] 単細胞解析を目指した新規バイオアッセイシステムの開発2009

    • Author(s)
      森喜一朗
    • Organizer
      日本機械学会第21回バイオエンジニアリング講演会
    • Place of Presentation
      札幌、札幌コンベンションセンター
    • Year and Date
      2009-01-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] Development of Newly Designed Bioassay System towards Single Cell Analysis2008

    • Author(s)
      森喜一朗
    • Organizer
      GPBE/NUS-Tohoku Graduate Student Conference in Bioengineering
    • Place of Presentation
      Singapore、Centre for Life Sciences(CeLS), NUS
    • Year and Date
      2008-12-08
    • Related Report
      2008 Annual Research Report
  • [Book] Chapter 23 Endothelial cell responses to fluid shear stress(From Methodology to Applications, in Single and two-Phase Flows on Chemical and Biomedical Engineering)2011

    • Author(s)
      Toshiro Ohashi, Masaaki Sato
    • Publisher
      Bentham e Books(in press)
    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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