Molecular Biological Analysis on Glomerular Function by Multi-Photon Confocal Laser Microscopy
| Project/Area Number |
20300163
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
NAKAMOTO Hiroshi Kawasaki Medical School, 医学部, 助教 (10299183)
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| Co-Investigator(Kenkyū-buntansha) |
OGASAWARA Yasuo 川崎医科大学, 医学部, 准教授 (10152365)
YADA Toyotaka 川崎医科大学, 医学部, 講師 (00210279)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2010: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2009: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2008: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
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| Keywords | 腎症 / 可視化 / 糖尿病 / 共焦点顕微鏡 / 濾過 |
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| Research Abstract |
We were able to visualize glomerular filtration in a rat. There was leakage of larger particles with the molecular weight of 70k Dal, which was found progressive even at the early stage of diabetes. The reason of this leakage was attributed to the structural changes of the slit membrane where filtration takes place. It is because alignment of podocin and CD2AP, component proteins of the slit membrane was heterogeneously damaged due to diabetes.
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Report
(4 results)
Research Products
(32 results)
