Molecular Biological Analysis on Glomerular Function by Multi-Photon Confocal Laser Microscopy

• Project/Area Number: 20300163
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kawasaki Medical School
• Principal Investigator: NAKAMOTO Hiroshi Kawasaki Medical School, 医学部, 助教 (10299183)
• Co-Investigator(Kenkyū-buntansha): OGASAWARA Yasuo 川崎医科大学, 医学部, 准教授 (10152365) ; YADA Toyotaka 川崎医科大学, 医学部, 講師 (00210279)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000); Fiscal Year 2010: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2009: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2008: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
• Keywords: 腎症 / 可視化 / 糖尿病 / 共焦点顕微鏡 / 濾過

Research Abstract

We were able to visualize glomerular filtration in a rat. There was leakage of larger particles with the molecular weight of 70k Dal, which was found progressive even at the early stage of diabetes. The reason of this leakage was attributed to the structural changes of the slit membrane where filtration takes place. It is because alignment of podocin and CD2AP, component proteins of the slit membrane was heterogeneously damaged due to diabetes.

Research Products

• [Journal Article] Visualisation of nephroprotective function of C-peptide in an early stage diabetic rat (2010)
  • Author(s): Hiroshi Nakamoto
  • Journal Title: 生体医工学 Volume: 48, Suppl.1 Pages: 291-291
• [Journal Article] In-vivoにおける糸球体濾過の可視化と定量化の試み (2010)
  • Author(s): 仲本博
  • Journal Title: 日本バイオイメージング Volume: 19(2) Pages: 82-83
• [Journal Article] Visualisation of nephroprotective function of C-peptide in an early stage diabetic rat (2009)
  • Author(s): Hiroshi Nakamoto, Yasuo Ogasawara, Fumihiko Kajiya
  • Journal Title: 生体医工学 48 Suppl.1 Pages: 291-292
• [Journal Article] In-vivoにおける糸球体濾過の可視化と定量化の試み (2009)
  • Author(s): 仲本博、小笠原康夫、梶谷文彦
  • Journal Title: 日本バイオイメージング 19(2) Pages: 82-83
• [Journal Article] In-vivo Visualisation of glomerular filtration by two-photon microscopy in a rat (2009)
  • Author(s): Hiroshi Nakamoto
  • Journal Title: IFMBE Proceedings 25 Pages: 1109-1110
• [Presentation] C-peptide Effects on Glomerular Filtration at the Early Stage of Diabetes (2011)
  • Author(s): Hiroshi Nakamoto, Yasuo Ogasawara, Fumihiko Kajiya
  • Organizer: The 75th Annual Scietntific Meeting of the Japanese Circulation Society
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2011-03-20
• [Presentation] Early diabetes and slit membrane (2011)
  • Author(s): Hiroshi Nakamoto, Kazuhiko Nakayama, Noriaki Emoto, Fumihiko Kajiya
  • Organizer: 第36回微小循環学会総会
  • Place of Presentation: 名古屋市立大学医学部付属病院(名古屋)
  • Year and Date: 2011-02-12
• [Presentation] 生体でのアデノシンによる糸球体輸出入細動脈の調節 (2011)
  • Author(s): 仲本博、矢田豊隆、小笠原康夫
  • Organizer: 第40回日本心脈管作動物質学会総会
  • Place of Presentation: かがわ国際会議場(香川)
  • Year and Date: 2011-02-05
• [Presentation] In-vivo Quantification of Glomerular Filtration in a Rat (2010)
  • Author(s): Hiroshi Nakamoto, Yasuo Ogasawara, Fumihiko Kajiya
  • Organizer: 19th International Conference of the Cardiovascular System Dynamics Society 2010
  • Place of Presentation: 九州大学医学部付属病院(福岡)
  • Year and Date: 2010-09-26
• [Presentation] In-vivo における糸球体濾過の可視化と定量化の試み (2010)
  • Author(s): 仲本博、小笠原康夫、梶谷文彦
  • Organizer: 日本バイオイメージング学会総会
  • Place of Presentation: 慶応大学日吉学舎(神奈川)
  • Year and Date: 2010-09-11
• [Presentation] In-vivoにおける糸球体濾過の可視化と定量化の試み (2010)
  • Author(s): 仲本博
  • Organizer: 日本バイオイメージング学会総会
  • Place of Presentation: 慶応大学日吉学舎(神奈川)
  • Year and Date: 2010-09-11
• [Presentation] In-vivo Visualisation Study of Renal Microcirculation in a Rat (2010)
  • Author(s): Hiroshi Nakamoto
  • Organizer: 6th World Congress on Biomechanics 2010
  • Place of Presentation: サンテック・シンガポール国際会議場(Singapore)
  • Year and Date: 2010-08-02
• [Presentation] Visualisation of nephroprotective function of C-peptide in an early stage diabetic rat (2010)
  • Author(s): Hiroshi Nakamoto, Yasuo Ogasawara, Fumihiko Kajiya
  • Organizer: 第49回日本生体医工学会大会
  • Place of Presentation: 大阪国際交流センター(大阪)
  • Year and Date: 2010-06-27
• [Presentation] C-peptide Effects on Glomerular Filtration at the Early Stage of Diabetes (2010)
  • Author(s): Hiroshi Nakamoto
  • Organizer: The 75th Annual Scietntific Meeting of the Japanese Circulation Society
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2010-03-20
• [Presentation] In-vivo Visualisation Study Revealed Hyperfiltration in an Early Diabetic Rat Can be Corrected with C-peptide (2010)
  • Author(s): Hiroshi Nakamoto, Yasuo Ogasawara, Fumihiko Kajiya
  • Organizer: The Japanese Circulation Society
  • Place of Presentation: Kyoto
  • Year and Date: 2010-03-07
• [Presentation] In-vivo measurement of sieving coefficients of a rat glomerular membrane (2010)
  • Author(s): Hiroshi Nakamoto, Yasuo Ogasawaram, Fumihiko Kajiya
  • Organizer: 日本微小循環学会
  • Place of Presentation: 大宮
  • Year and Date: 2010-02-27
• [Presentation] 生理的条件下での腎糸球体濾過機能定量 (2010)
  • Author(s): 仲本博
  • Organizer: 第22回バイオエンジニアリング講演会
  • Place of Presentation: 岡山
  • Year and Date: 2010-01-10
• [Presentation] 生理的条件下での腎糸球体濾過機能定量 (2010)
  • Author(s): 仲本博、小笠原康夫、梶谷文彦
  • Organizer: 第22回バイオエンジニアリング講演会
  • Place of Presentation: 於岡山理科大学, 岡山
• [Presentation] 糖尿病における糸球体濾過には早期から障害が生じている (2009)
  • Author(s): 仲本博
  • Organizer: 第32回日本バイオレオロジー学会年会
  • Place of Presentation: 桐生
  • Year and Date: 2009-06-05
• [Presentation] 早期糖尿病ラットにおける過濾過の可視化 (2009)
  • Author(s): 仲本博
  • Organizer: 日本生体医工学会
  • Place of Presentation: 東京
  • Year and Date: 2009-04-25
• [Presentation] Progressive hyperfiltration at the early stage of diabetes in a rat (2009)
  • Author(s): Nakamoto Hiroshi, Ogasawara Yasuo, Kajiya Fumihiko
  • Organizer: The 73st Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: Osaka
  • Year and Date: 2009-03-20
• [Presentation] PROGRESSION OF HYPERFILTRATION IN AN EARLY DIABETIC RAT WAS VISUALISED BY MULTIPHOTON MICROSCOPY (2009)
  • Author(s): Nakamoto Hiroshi, Ogasawara Yasuo, Kajiya Fumihiko
  • Organizer: The 36th Congress of the International Union of Physiological Sciences
  • Place of Presentation: Kyoto
• [Presentation] 糖尿病における糸球体濾過には早期から障害が生じている (2009)
  • Author(s): 仲本博、小笠原康夫、梶谷文彦
  • Organizer: 第32回日本バイオレオロジー学会年会
  • Place of Presentation: 群馬
• [Presentation] 早期糖尿病ラットにおける過濾過の可視化 (2009)
  • Author(s): 仲本博、小笠原康夫、梶谷文彦
  • Organizer: 第48回日本生体医工学会大会
  • Place of Presentation: 東京
• [Presentation] Existence of Hyperfiltration in an Early Diabetic Rat was Visualised by Multiphoton Microscopy (2008)
  • Author(s): Nakamoto Hiroshi, Ogasawara Yasuo, Kajiya Fumihiko
  • Organizer: AHA Scientific Sessions(Circulation.2008;118:S_337.)
  • Place of Presentation: New Orleans
  • Year and Date: 2008-11-10
• [Presentation] Existence of hyperfiltration in an early diabetic rat was visualised by multiphoton microscopy. (2008)
  • Author(s): Hiroshi Nakamoto
  • Organizer: American Heart Association, scientific sessions 2008
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2008-11-10
• [Presentation] 早期糖尿病の腎糸球体におけるhyperfiltrationの可視化による検討 (2008)
  • Author(s): 仲本博,小笠原康夫
  • Organizer: 第31回日本バイオレオロジー学会年会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-05
• [Presentation] In-vivo visualisation of disturbance of glomerular hyperfiltration in an early diabetic rat (2008)
  • Author(s): Nakamoto Hiroshi, Ogasawara Yasuo, Kajiya Fumihiko
  • Organizer: 第51回日本腎臓学会学術総会
  • Place of Presentation: Fukuoka
  • Year and Date: 2008-06-01
• [Presentation] In-vivo visualisation of disturbance of glomerular hyperfiltration in an early diabetic rat. (2008)
  • Author(s): Hiroshi Nakamoto
  • Organizer: 第51回日本腎臓学会学術総会
  • Place of Presentation: Fukuoka, JAPAN
  • Year and Date: 2008-06-01
• [Presentation] 糸球体濾過の分子レベルの蛍光可視化 (2008)
  • Author(s): 仲本博,小笠原康夫
  • Organizer: 第47回日本生体医工学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-05-08
• [Presentation] In-vivo Visualisation Study of Renal Microcirculation in a Rat
  • Author(s): Hiroshi Nakamoto, Yasuo Ogasawara, Fumihiko Kajiya
  • Organizer: 6th World Congress on Biomechanics 2010
  • Place of Presentation: サンテック・シンガポール国際会議場(Singapore)
• [Remarks] ホームページ
  • URL: http://www.kawasaki-m.ac.jp/med/study/info.php?id=303