Spetiotemporal analyses of RNA with biofunctional probes
| Project/Area Number |
20300167
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
KOBATAKE Eiry 東京工業大学, 大学院・生命理工学研究科, 准教授 (00225484)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2009: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2008: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Keywords | RNA解析 / 生体機能分子プローブ / ルシフェラーゼ / ARM-ペプチド / 構造変化 / ARM-ペフチド / 生体機能分子プローグ / ルミフェラーゼ |
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| Research Abstract |
The objective of this study is to develop a method for quantitative analyses of specific RNA in cells. The novel recombinant protein was constructed by connecting fragments of separated bioluminescent enzyme to both ends of peptide which is induced structural change by binding with RNA. RNA probes which bind both the constructed protein and target RNA were also synthesized. Through the use of these components, target RNA could be detected with high efficiency. Furthermore, RNAs in cells could be successfully detected.
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Report
(4 results)
Research Products
(22 results)
