Development of Cancer Imaging System by Using Cell Signal-Responsive Molecular System
| Project/Area Number |
20300168
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Kyushu University |
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Principal Investigator |
KATAYAMA Yoshiki Kyushu University, 大学院・工学研究院, 教授 (70284528)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥19,890,000 (Direct Cost: ¥15,300,000、Indirect Cost: ¥4,590,000)
Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2009: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2008: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
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| Keywords | 遺伝子送達 / プロテインキナーゼ / 細胞内情報伝達 / DDS / in vivoイメージング / 細胞内シグナル / ペプチド / 遺伝子キャリヤー / 遺伝子治療 / バイオイメージング / 診断 |
|---|
| Research Abstract |
In this research, molecular systems which can visualize an activity of target protein kinase have been developed. First of all, we successfully developed protein kinase Cα-responsive system. The system visualized various tumors in mice. Second, fluorescence probe of PKCα was also prepared. In this system, fluorescence labeled polyanion was used instead of reporter gene. Another fluorescent probe of PKCα was also designed by using an electrostatic complex between quencher-labeled condroitin sulfate and a PAMAM dendrimer labeled with fluorophre and substrate peptide. In addition, signal responsibility of the reporter gene regulation system improved dramatically by changing the polymer backbone from neutral polyacrylamide to polyethylene imine. Various novel substrate specific to various protein kinases relating to cancer functions have also be developed successfully.
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Report
(4 results)
Research Products
(112 results)
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[Journal Article] Hepatoma-targeted gene delivery using a tumor cell-specific gene regulation system combined with a human liver cell-specific bionanocapsule2010
Author(s)
J-H.Kang, J.Oishi, J-H.Kim, M.Ijuin, R.Toita, B.Jun, D.Asai, T.Mori, T.Niidome, K.Tanizawa, S. Kuroda, Y. Katayama
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Journal Title
Nanomedicine 6
Pages: 583-589
Related Report
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[Journal Article] Specific transgene expression in HIV-infected cells using protease-cleavable transcription regulator2010
Author(s)
D.Asai, M.Kuramoto, Y.Shoji, K.B.Kodama, J-H.Kagng, K.Kawamura, H.Miyoshi, T.Mori, T.Niidome, H.Nakashima, Y.Katayama
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Journal Title
J.Control.Release 141
Pages: 52-61
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[Journal Article] Design of polymeric carriers for cancer-specific gene targeting : Utilization of abnormal protein kinase Cα activation in cancer cells2008
Author(s)
J-H.Kang, D.Asai, J-H.Kim, T.Mori, R.Toita, T.Tomiyama, Y.Asami, J.Oishi, Y.T.Sato, T.Niidome, B.Jun, H.Nakashima, Y.Katayama
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Journal Title
J.Am.Chem.Soc. 130
Pages: 14906-14907
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