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Functionalization of thermoresponsive nano-interfaces and thermally regulated detachment behaviors of adherent proteins and cells

Research Project

Project/Area Number 20300169
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionTokyo Women's Medical University

Principal Investigator

OKANO Teruo  Tokyo Women's Medical University, 医学部, 教授 (00130237)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2010: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2009: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2008: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Keywordsインテリジェント材料 / 表面機能化 / 温度応答性 / 細胞シート / ナノ表面 / 精密重合 / poly(N-isopropylacrylamide) / 温度応答性表面 / 細胞シート工学 / RAFT重合 / 高分子ブラシ / リビングラジカル重合
Research Abstract

In this study, surface-initiated RAFT polymerization was demonstrated to materialize new type thermoresponsive PIPAAm brush surfaces for cell sheet harvest. The molecular weight and graft density of grafted PIPAAm chains was controllable through this grafting method. Therefore, optimal thermoresponsive surfaces were able to be designed for cell sheet preparation. Furthermore, RAFT-mediated block copolymerization achieved the stripe-like micropatterning of thermoresponsive PIPAAm domains and cell-repellent polymer domains. Human fibroblasts were aligned on the micropatterned polymer brush surfaces and the aligned cells were harvested as a cell sheet with well-controlled orientational structures.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (19 results)

All 2011 2010 2009 Other

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (1 results) Remarks (2 results)

  • [Journal Article] Thermo-responsive polymer brushes as intelligent biointerfaces : preparation via ATRP and characterization2011

    • Author(s)
      K.Nagase, M.Watanabe, A.Kikuchi, M.Yamato, T.Okano
    • Journal Title

      Macromol.Biosci. 11巻

      Pages: 400-409

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Thermally-modulated on/off-adsorption materials for pharmaceutical protein purification2011

    • Author(s)
      K.Nagase, J.Kobayashi, A.Kikuchi, Y.Akiyama, H.Kanazawa, T.Okano
    • Journal Title

      Biomaterials

      Volume: 32 Pages: 619-627

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Thermo-responsive protein adsorbing materials for purifying pharmaceutical protein on exposed charging surface2011

    • Author(s)
      K.Nagase, S.F.Yuk, J.Kobayashi, A.Kikuchi, Y.Akiyama, H.Kanazawa, T.Okano
    • Journal Title

      J.Mater.Chem.

      Volume: 21 Pages: 2590-2593

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Thermo-responsive polymer brushes as intelligent biointerfaces : preparation via ATRP and characterization2011

    • Author(s)
      K.Nagase, M.Watanabe, A.Kikuchi, M.Yamato, T.Okano
    • Journal Title

      Macromol.Biosci.

      Volume: 11 Pages: 400-409

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Controlled chain length and graft density of thermoresponsive polymer brushes for optimizing cell sheet harvest2010

    • Author(s)
      H.Takahashi, M.Nakayama, M.Yamato, T.Okano
    • Journal Title

      Biomacromolecules 11巻

      Pages: 1991-1999

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Preparation of thermoresponsive anionic copolymer brush surfaces for separating basic biomolecules2010

    • Author(s)
      K.Nagase, J.Kobayashi, A.Kikuchi, Y.Akiyama, H.Kanazawa, M.Annaka, T.Okano
    • Journal Title

      Biomacromolecules 11巻

      Pages: 215-223

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Thermoresponsive Polymer Brush Surfaces with Hydrophobic Groups for All-Aqueous Chromatography2010

    • Author(s)
      K.Nagase, M.Kumazaki, H.Kanazawa, J.Kobayashi, A.Kikuchi, Y.Akiyama, M.Annaka, T.Okano
    • Journal Title

      ACS Applied Materials & Interfaces

      Volume: 2 Pages: 1247-1253

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides2010

    • Author(s)
      A.Mizutani, K.Nagase, A.Kikuchi, H.Kanazawa, Y.Akiyama, J.Kobayashi, M.Annaka, T.Okano
    • Journal Title

      J.Chromatogr.A

      Volume: 1217 Pages: 5978-5985

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effective separation of peptides using highly dense thermo-responsive polymer brush-grafted porous polystyrene beads2010

    • Author(s)
      A.Mizutani, K.Nagase, A.Kikuchi, H.Kanazawa, Y.Akiyama, J.Kobayashi, M.Annaka, T.Okano
    • Journal Title

      J.Chromatogr.B

      Volume: 878 Pages: 2191-2198

    • NAID

      10028040122

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Controlled chain length and graft density of thermoresponsive polymer brushes for optimizing cell sheet harvest2010

    • Author(s)
      H.Takahashi, M.Nakayama, M.Yamato, T.Okano
    • Journal Title

      Biomacromolecules

      Volume: 11 Pages: 1991-1999

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Thermo-responsive polymer brush-grafted porous polystyrene beads for all-aqueous chromatography2010

    • Author(s)
      水谷文、長瀬健一、菊池明彦、金沢秀子、秋山義勝、小林純、安中雅彦、岡野光夫
    • Journal Title

      Journal of Chromatography A 1217

      Pages: 522-522

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Preparation of thermoresponsive anionic copolymer brush surfaces for Separating basic biomolecules2010

    • Author(s)
      長瀬健一、小林純、菊池明彦、秋山義勝、金沢秀子、安中雅彦、岡野光夫
    • Journal Title

      Biomacromolecules 11

      Pages: 215-215

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Temperature-responsive intelligent interfaces for biomolecular separation and cell sheet engineering2009

    • Author(s)
      K.Nagase, J.Kobayashi, T.Okano
    • Journal Title

      Journal of the Royal Society Interface 6巻

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Micropatterned thermoresponsive polymer brush surfaces for fabricating cell sheets with well-controlled orientational structures

    • Author(s)
      H.Takahashi, M.Nakayama, K.Itoga, M.Yamato, T.Okano
    • Journal Title

      Biomacromolecules (in press)

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Micropatterned thermoresponsive polymer brush surfaces for fabricating cell sheets with well-controlled orientational structures

    • Author(s)
      H.Takahashi, M.Nakayama, K.Itoga, M.Yamato, T.Okano
    • Journal Title

      Biomacromolecules

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Temperature-responsive intelligent interfaces for biomolecular separation and cell sheet engineering

    • Author(s)
      長瀬 健一, 小林 純, 岡野 光夫
    • Journal Title

      Journal of the Royal Society Interface (In press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Temperature Responsive Polymeric Surfaces for Biomedical Applications2009

    • Author(s)
      岡野光夫
    • Organizer
      14^<th> International Symposium on Recent Advances in Drug Delivery Systems
    • Place of Presentation
      アメリカ合衆国
    • Year and Date
      2009-02-17
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Remarks] ホームページ

    • URL

      http://www.twmu.ac.jp/ABMES/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.twmu.ac.jp/ABMES/ja/works

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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