Effects of amino acid administration and exercise on the regulation of the muscle protein and energy metabolism.

• Project/Area Number: 20300216
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Sports science
• Research Institution: Nagoya University
• Principal Investigator: SHIMOMURA Yoshiharu Nagoya University, 生命農学研究科, 教授 (30162738)
• Co-Investigator(Kenkyū-buntansha): KITAURA Yasuyuki 名古屋大学, 大学院・生命農学研究科, 助教 (90442954)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2010: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2009: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2008: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
• Keywords: 分岐鎖アミノ酸(BCAA) / 分岐鎖α-ケト酸脱水素酵素(BCKDH) / BCKDHキナーゼ / 廃用性筋萎縮 / ラット / ヒラメ筋 / タンパク質合成促進因子 / ミトコンドリア / 高脂肪食 / インスリン抵抗性 / 耐糖能 / ロイシン / 後肢懸垂 / 筋タンパク質 / BCAA / 分岐鎖α-ケト酸脱水素酵素複合体 / BCAA食 / 総RNA

Research Abstract

The metabolic control by nutrition and exercise may play important roles in the health promotion, and its correct information in regard to the responsible mechanisms for the effects are required. In the present studies, we addressed to clarify effects of branched-chain amino acid (BCAA) administration and exercise on the health promotion and the responsible mechanisms for the effects : the detailed aims were to clarify (1) the regulatory mechanism responsible for the BCAA metabolism, (2) the effects of BCAA administration on the disuse-induced muscle atrophy, and (3) the function of BCAA on the glucose metabolism. We found the evidence that suggests the existence of an unknown factor which may regulate the BCAA catabolism through inactivation of the branched-chain α-keto acid dehydrogenase kinase in rat liver mitochondria. In the study for the disuse-induced muscle atrophy, we found that the feeding of 5% BCAA diet attenuated disuse-induced decreases in the factors that promote cell growth and protein synthesis, although it did not affect the muscle atrophy itself and amounts of the components in the ubiquitin-proteasome system. Furthermore, we found that the plasma BCAA concentrations may play an important role in the normal glucose tolerance.

Research Products

• [Journal Article] Clofibrate-induced reduction of plasma branched-chain amino acid concentrations impairs glucose tolerance in rats. (2011)
  • Author(s): Kadota, Y., Kazama, S., Bajotto, G., Kitaura, Y., Shimomura Y.
  • Journal Title: J.Parenter.Enteral Nutr. In press
  • Peer Reviewed
• [Journal Article] Effect of branched-chain amino acid supplementation during unloading on regulatory components of protein synthesis in atrophied soleus muscles. (2011)
  • Author(s): Bajotto, G., Sato, Y., Kitaura, Y., Shimomura Y.
  • Journal Title: Eur.J.Appl.Physiol. In press
  • Peer Reviewed
• [Journal Article] Effect of branched-chain amino acid supplementation during unloading on regulatory components of protein synthesis in atrophied soleus muscles. (2011)
  • Author(s): Bajotto, G., et al.
  • Journal Title: Eur.J.Appl.Physiol. Volume: (In press)(掲載確定)
  • Peer Reviewed
• [Journal Article] Branched-chain amino acid supplementation before squat exercise and delayed-onset muscle soreness. (2010)
  • Author(s): Shimomura Y., Inaguma, A., Watanabe, S., Yamamoto, Y., Muramatsu, Y., Bajotto, G., Sato, J., Shimomura, N., Kobayashi, H., Mawatari, K.
  • Journal Title: Int.J.Sport Nutr.Exerc.Metab. 20 Pages: 236-244
  • Peer Reviewed
• [Journal Article] Branched-chain amino acid supplementation before squat exercise and delayedonset muscle soreness. (2010)
  • Author(s): Shimomura, Y., et al.
  • Journal Title: Int.J.Sport Nutr.Exerc.Metab. Volume: 20 Pages: 236-244
  • Peer Reviewed
• [Journal Article] Decreased enzyme activity and contents of hepatic branched-chain α-keto acid dehydrogenase complex subunits in a rat model for type 2 diabetes mellitus. (2009)
  • Author(s): Bajotto, G., Murakami, T., Nagasaki, M., Sato, Y., Shimomura Y.
  • Journal Title: Metabolism 58 Pages: 1489-1495
  • Peer Reviewed
• [Journal Article] Effects of squat exercise and branched-chain amino acid supplementation on plasma free amino acid concentrations in young women. (2009)
  • Author(s): Shimomura Y., Kobayashi, H., Mawatari, K., Akita, K., Inaguma, A., Watanabe, S., Bajotto, G., Sato, J.
  • Journal Title: J.Nutr.Sci.Vitaminol. 55 Pages: 288-291
  • NAID: 10025971509
  • Peer Reviewed
• [Journal Article] Inhibition of branched-chain α-ketoacid dehydrogenase kinase by thiamine pyrophosphate at different potassium ionic levels. (2009)
  • Author(s): Akita, K., Fujimura, Y., Bajotto, G., Shimomura Y.
  • Journal Title: Biosci.Biotechnol.Biochem. 73 Pages: 1189-1191
  • NAID: 10027541478
  • Peer Reviewed
• [Journal Article] Modified method for purifying rat liver branched-chain α-ketoacid dehydrogenase complex. (2009)
  • Author(s): Fujimura, Y., Muramatsu, Y., Akita, K., Bajotto, G, Shimomura Y.
  • Journal Title: Biosci.Biotechnol.Biochem. 73 Pages: 766-768
  • NAID: 10027539281
  • Peer Reviewed
• [Journal Article] 分岐鎖アミノ酸(BCAA)代謝の調節機構 (2009)
  • Author(s): 下村吉治
  • Journal Title: 化学と生物 47 Pages: 480-485
  • NAID: 10026435927
• [Journal Article] Inhibition of branched-chain α-ketoacid dehydrogenase kinase by thiamine pyrophosphate at different potassium ionic levels (2009)
  • Author(s): Akita, K., et al.
  • Journal Title: Biosci.Biotechnol.Biochem. 73 Pages: 1189-1191
  • Peer Reviewed
• [Journal Article] Effects of squat exercise and branched-chain amino acid supplementation on plasma free amino acid concentrations in young women (2009)
  • Author(s): Shimomura, Y., et al.
  • Journal Title: J.Nutr.Sci.Vitaminol. 55 Pages: 288-291
  • NAID: 10025971509
  • Peer Reviewed
• [Journal Article] Decreased enzyme activity and contents of hepatic branched-chain α-keto acid dehydrogenase complex subunits in a rat model for type 2 diabetes mellitus (2009)
  • Author(s): Bajotto, G., et al.
  • Journal Title: Metabolism 58 Pages: 1489-1495
  • Peer Reviewed
• [Journal Article] Modified method for purifying rat liver branched-chain α-ketoacid dehydrogenase complex. (2009)
  • Author(s): Fujimura, Y., et al.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry 73 Pages: 766-768
  • Peer Reviewed
• [Journal Article] The effects of endotoxin on plasma free amino acid concentrations in rats. (2008)
  • Author(s): Asai, Y., Bajotto, G., Yoshizato, H., Hamada, K., Higuchi, T., Shimomura Y.
  • Journal Title: J.Nutr.Sci.Vitaminol. 54 Pages: 460-466
  • NAID: 110007007844
  • Peer Reviewed
• [Journal Article] Regulation of branched-chain amino acid catabolism in rat models for spontaneous type 2 diabetes mellitus. (2008)
  • Author(s): Kuzuya, T., Katano, Y., Nakano, I., Hirooka, Y., Itoh, A., Ishigami, M., Hayashi, K., Honda, T., Goto, H., Fujita, Y., Shikano, R., Muramatsu, Y., Bajotto, G., Tamura, T., Tamura, N., Shimomura Y.
  • Journal Title: Biochem.Biophys.Res.Commun. 373 Pages: 94-98
  • Peer Reviewed
• [Journal Article] Regulation of branched-chain amino acid catabolism in rat models for spontaneous type 2 diabetes mellitus. (2008)
  • Author(s): Kuzuya, T., et al.
  • Journal Title: Biochemical & Biophysical Research Communications 373 Pages: 94-98
  • Peer Reviewed
• [Journal Article] The effects of endotoxin on plasma free amino acid concentrations in rats. (2008)
  • Author(s): Asai, Y., et al.
  • Journal Title: Journal of Nutritional Science and Vitaminology 54 Pages: 460-466
  • Peer Reviewed
• [Presentation] 分岐鎖アミノ酸(BCAA)代謝の調節:Branched-chain α-keto acid dehydrogenase (BCKDH) kinaseの不活性化因子 (2010)
  • Author(s): 風間駿輔
  • Organizer: 日本アミノ酸学会第4回学術集会
  • Place of Presentation: ホテルサンシャイン鬼怒川(日光市)
  • Year and Date: 2010-09-17
• [Presentation] 分岐鎖アミノ酸(BCAA)代謝の調節:Branched-chain α-keto acid dehydrogenase (BCKDH) kinaseの不活性化因子 (2010)
  • Author(s): 風間駿輔、白井伸太朗、土本隼、北浦靖之、下村吉治
  • Organizer: 日本アミノ酸学会第4回学術集会
  • Place of Presentation: ホテルサンシャイン鬼怒川(栃木県日光市)
  • Year and Date: 2010-09-17
• [Presentation] ラット後肢懸垂による筋萎縮時のタンパク質合成調節因子に対する分岐鎖アミノ酸(BCAA)添加食摂取の影響 (2010)
  • Author(s): Gustavo Bajotto
  • Organizer: 第65回日本体力医学会大会
  • Place of Presentation: 千葉商科大学(市川市)
  • Year and Date: 2010-09-16
• [Presentation] 低血漿分岐鎖アミノ酸(BCAA)濃度による耐糖能の低下 (2010)
  • Author(s): 門田吉弘
  • Organizer: 日本外科代謝栄養学会第47回学術集会
  • Place of Presentation: 横浜産貿ホール(横浜市)
  • Year and Date: 2010-09-16
• [Presentation] ラット後肢懸垂による筋萎縮時のタンパク質合成調節因子に対する分岐鎖アミノ酸(BCAA)添加食摂取の影響 (2010)
  • Author(s): Gustavo Bajotto, 高柳洋、下村吉治
  • Organizer: 第65回日本体力医学会大会
  • Place of Presentation: 千葉商科大学(千葉県市川市)
  • Year and Date: 2010-09-16
• [Presentation] 低血漿分岐鎖アミノ酸(BCAA)濃度による耐糖能の低下 (2010)
  • Author(s): 門田吉弘、G.Bajotto, 他
  • Organizer: 日本外科代謝栄養学会第47回学術集会
  • Place of Presentation: 横浜産貿ホール(神奈川県横浜市)
  • Year and Date: 2010-07-09
• [Presentation] Very low density lipoprotein-apob100 overproduction is associated with the systemic and hepatic inflammation in Otsuka Long-Evans Tokushima fatty rat, an animal model of type 2 diabetes. (2010)
  • Author(s): Bolin Qin
  • Organizer: American Diabetes Association 70th Scientific Session
  • Place of Presentation: Orlando, Florida, USA
  • Year and Date: 2010-06-27
• [Presentation] Branched-chain α-keto acid dehydrogenase (BCKDH) kinaseの活性測定 (2010)
  • Author(s): 風間駿輔
  • Organizer: 第64回日本栄養・食糧学会大会
  • Place of Presentation: アスティー徳島(徳島市)
  • Year and Date: 2010-05-23
• [Presentation] Branched-chain α-keto acid dehydrogenase (BCKDH) kinaseの活性測定 (2010)
  • Author(s): 風間駿輔, 他
  • Organizer: 第64回日本栄養・食糧学会大会
  • Place of Presentation: アスティー徳島(徳島県徳島市)
  • Year and Date: 2010-05-23
• [Presentation] 2型糖尿病モデルラット(ZDFラット)における分岐鎖アミノ酸(BCAA)代謝 (2010)
  • Author(s): 土居崎正雄
  • Organizer: 第64回日本栄養・食糧学会大会
  • Place of Presentation: アスティー徳島(徳島市)
  • Year and Date: 2010-05-22
• [Presentation] BCAA代謝に対する高脂肪食摂取の影響 (2010)
  • Author(s): 門田吉弘
  • Organizer: 第64回日本栄養・食糧学会大会
  • Place of Presentation: アスティー徳島(徳島市)
  • Year and Date: 2010-05-22
• [Presentation] BCAA代謝に対する高脂肪食摂取の影響 (2010)
  • Author(s): 門田吉弘、北浦靖之、下村吉治
  • Organizer: 第64回日本栄養・食糧学会大会
  • Place of Presentation: アスティー徳島(徳島県徳島市)
  • Year and Date: 2010-05-22
• [Presentation] 2型糖尿病モデルラット(ZDFラット)における分岐鎖アミノ酸(BCAA)代謝 (2010)
  • Author(s): 土居崎正雄、下村吉治, 他
  • Organizer: 第64回日本栄養・食糧学会大会
  • Place of Presentation: アスティー徳島(徳島県徳島市)
  • Year and Date: 2010-05-22
• [Presentation] Regulation of hepatic branched-chain α-keto acid dehydrogenase kinase in a rat model for type 2 diabetes mellitus at different stages of the disease. (2010)
  • Author(s): 土居崎正雄
  • Organizer: Experimental Biology 2010
  • Place of Presentation: Anaheim, California, USA
  • Year and Date: 2010-04-27
• [Presentation] The concentrations of plasma branched-chain amino acids affect glucose tolerance in rats. (2010)
  • Author(s): 門田吉弘
  • Organizer: Experimental Biology 2010
  • Place of Presentation: Anaheim, California, USA
  • Year and Date: 2010-04-26
• [Presentation] Lowered plasma branched-chain amino acid concentrations impairs glucose tolerance in rats. (2009)
  • Author(s): 門田吉弘
  • Organizer: 19^<th> International Congress of Nutrition
  • Place of Presentation: タイ、バンコク
  • Year and Date: 2009-10-08
• [Presentation] Lowered plasma branched-chain amino acid concentrations impairs glucose tolerance in rats (2009)
  • Author(s): 門田吉弘, et al.
  • Organizer: 19^<th> International Congress of Nutrition
  • Place of Presentation: タイ、バンコク
  • Year and Date: 2009-10-08
• [Presentation] 耐糖能に対する血漿BCAA濃度低下の影響 (2009)
  • Author(s): 門田吉弘
  • Organizer: 日本アミノ酸学会第3回学術集会
  • Place of Presentation: 京都府立大学大学院(京都市)
  • Year and Date: 2009-09-30
• [Presentation] 耐糖能に対する血漿BCAA濃度低下の影響 (2009)
  • Author(s): 門田吉弘, 他
  • Organizer: 日本アミノ酸学会第3回学術集会
  • Place of Presentation: 京都府立大学大学院
  • Year and Date: 2009-09-30
• [Presentation] 分岐鎖アミノ酸(BCAA)の代謝調節と生理機能 (2009)
  • Author(s): 下村吉治
  • Organizer: 日本栄養・食糧学会第63回大会
  • Place of Presentation: 長崎ブリックホール(長崎市)
  • Year and Date: 2009-05-22
• [Presentation] 分岐鎖アミノ酸 (BCAA) の代謝調節と生理機能 (2009)
  • Author(s): 下村吉治
  • Organizer: 日本栄養・食糧学会第63回大会
  • Place of Presentation: 長崎
  • Year and Date: 2009-05-22
• [Presentation] Branched-chain alpha-ketoacid dehydrogenase complex (BCKDC) : purification from rat liver and inhibition by thiamin pyrophosphate (TPP) (2009)
  • Author(s): 下村吉治
  • Organizer: Experimental Biology 2009
  • Place of Presentation: New Orleans, FL, USA
  • Year and Date: 2009-04-20
• [Presentation] Branched-chain alpha-ketoacid dehydrogenase complex (BCKDC) : purification from rat liver and inhibition by thiamin pyrophosphate (TPP) (2009)
  • Author(s): 下村吉治, et al.
  • Organizer: 実験生物学会EB2009
  • Place of Presentation: 米国ニューオリンズ
  • Year and Date: 2009-04-20
• [Presentation] ラット後肢懸垂による筋萎縮に対する食餌中の乳清タンパク質とカゼインの影響の比較 (2008)
  • Author(s): 小寺体佳, 他
  • Organizer: 第62回日本栄養・食糧学会大会
  • Place of Presentation: 女子栄養大学(坂戸市)
  • Year and Date: 2008-05-04
• [Presentation] 中鎖脂肪酸投与による血清ケトン体濃度と肝臓分岐鎖α-ケト酸脱水素酵素(BCKDH)複合佳活佳への影響 (2008)
  • Author(s): 永田宏樹, 他
  • Organizer: 第62回日本栄養・食糧学会大会
  • Place of Presentation: 女子栄養大学(坂戸市)
  • Year and Date: 2008-05-04
• [Presentation] 耐糖能に対する血中BCAA濃度の影響 (2008)
  • Author(s): 門田吉弘, 他
  • Organizer: 第62回日本栄養・食糧学会大会
  • Place of Presentation: 女子栄養大学(坂戸市)
  • Year and Date: 2008-05-04
• [Remarks] ホームページ等
  • URL: http://www.agr.nagoya-u.ac.jp/~nutr/shimomura/index.html