Analysis of food ingredient's effect based on the gene expression
| Project/Area Number |
20300243
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Eating habits, studies on eating habits
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| Research Institution | Ochanomizu University |
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Principal Investigator |
OTSUKA Yuzuru Ochanomizu University, 大学院・人間文化創成科学研究科, 教授 (20135833)
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| Co-Investigator(Kenkyū-buntansha) |
UETA Etsuko 鳥取大学, 医学部, 講師 (40335526)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2010: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2009: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2008: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Keywords | DNAマイクロアレイ / 遺伝子発現 / 食品機能 / HepG2 / HUCF2 / HUC-F2 / 機能性食品素材 / PQQ / 神経細胞 / β細胞 |
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| Research Abstract |
To investigate the food effect on the health, food ingredient's effects on the gene expression were analyzed using DNA microarray, pass way analysis and real time PCR methods. The HepG2 cells derived from human hepatoma and HUCF2 cells from human normal fibroblast were incubated with elder, human mother's milk, alpha-tocotrienol, PQQ etc. RNA was isolated and labeled and analyzed using Agilent DNA microarray. Oxidative phosphorylation pass way was increased by elder addition. EGF signaling pass way was increased by mother's milk addition. FGF signaling pass way was increased by alpha-tocotrienol. This pass way activates Map kinase signaling pass way to cause cell growth on HUCF2cell.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Association with lifestyle factor, polymorphisms in Adiponectin, Perilipin and Hormone Sensitive Lipase, and clinical markers in Japanese male2010
Author(s)
Y Sone, K Yamaguchi, A Fujiwara, T Kido, K Kawahara, A Ishiwaki, K Kondo, Y Morita, N Tominaga, Y Otsuka.
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Journal Title
J Nutr Sci Vitaminol. 56(2)
Pages: 123-131
Related Report
Peer Reviewed
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[Journal Article] Association of lifestyle factors, polymorphisms in adiponectin, perilipin and hormone sensitive lipase, and clinical markers in Japanese males.2010
Author(s)
Sone Y, Yamaguchi K, Fujiwara A, Kido T, Kawahara K, Ishiwaki A, Kondo K, Morita Y, Tominaga N, Otsuka Y.
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Journal Title
J Nutr Sci Vitamino1 (Tokyo).
Volume: 56(2)
Pages: 123-31
NAID
Related Report
Peer Reviewed
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[Journal Article] Association with lifestyle factor, polymorphisms in Adiponectin, Perilipin and Hormone Sensitive Lipase, and clinical markers in Japanese male2010
Author(s)
Yasuko Sone, Keiko Yamaguchi, Aya Fujiwara, Toshimi Kido, Kazuo Kawahara, Asako Ishiwaki, Kazuo Kondo, Yutaka Morita, Noriko Tominaga, Yuzuru Otsuka
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Journal Title
Journal of Nutrition Science Vitaminology 56
Pages: 123-131
Related Report
Peer Reviewed
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[Journal Article] Effect of salt concentration on the reaction rate of Glc with amino acids, peptides and proteins.2009
Author(s)
K Yamaguchi, Y Noumi, K Nakajima, C Nagatsuka, H Aizawa, R Nakawaki, E Mizude, Y Otsuka, T Homma, Nguyen VC.
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Journal Title
Biosci. Biotech. Biochem 73(11)
Pages: 2379-2383
NAID
Related Report
Peer Reviewed
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