| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2010: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2009: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2008: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Research Abstract |
To investigate the food effect on the health, food ingredient's effects on the gene expression were analyzed using DNA microarray, pass way analysis and real time PCR methods. The HepG2 cells derived from human hepatoma and HUCF2 cells from human normal fibroblast were incubated with elder, human mother's milk, alpha-tocotrienol, PQQ etc. RNA was isolated and labeled and analyzed using Agilent DNA microarray. Oxidative phosphorylation pass way was increased by elder addition. EGF signaling pass way was increased by mother's milk addition. FGF signaling pass way was increased by alpha-tocotrienol. This pass way activates Map kinase signaling pass way to cause cell growth on HUCF2cell.
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