Biochemical analysis of post-replication repair pathway involved in induced mutagenesis
Project/Area Number |
20310030
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Risk sciences of radiation/Chemicals
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Research Institution | Nagoya University (2011) Hiroshima University (2008-2010) |
Principal Investigator |
MASUDA Yuji 名古屋大学, 環境医学研究所, 准教授 (30273866)
|
Co-Investigator(Kenkyū-buntansha) |
KAMIYA Kenji 広島大学, 原爆放射線医科学研究所, 教授 (60116564)
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Project Period (FY) |
2008 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2009: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2008: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
|
Keywords | 複製後修復 / 損傷乗り越え DNA 合成 / 突然変異誘発 / PCNA / RAD6-RAD18 / HLTF / 損傷乗り越えDNA合成 / ユビキチン / SHPRH / 突然変異 |
Research Abstract |
DNA lesions produced by ionizing radiation and environmental mutagen inhibit DNA replication. Stalling replication would lead to cell death if it persisted. Cells have mechanisms (post-replication repair, PRR) to restore DNA replication to survive against DNA-damaging agents. PRR is involved in induced mutagenesis. In this study, we purifiedenzymes required for PRR, reconstituted biochemical reactions with the enzymes and analyzed molecular mechanisms of the PRR.
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Report
(6 results)
Research Products
(93 results)
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[Journal Article] Regulation of DNA polymerase POLD4 influences genomic instability in lung cancer.2010
Author(s)
Huang QM, Tomida S, Masuda Y, Arima C, Cao K, Kasahara TA, Osada H, Yatabe Y, Akashi T, Kamiya K, Takahashi T, Suzuki M.
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Journal Title
Cancer Res.
Volume: 70
Pages: 8407-8416
Related Report
Peer Reviewed
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[Journal Article] Regulation of DNA polymerase POLD4 influences genomic instability in lung cancer.2010
Author(s)
Huang QM, Tomida S, Masuda Y, Arima C, Cao K, Kasahara TA, Osada H, Yatabe Y, Akashi T, Kamiya K, Takahashi T, Suzuki M.
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Journal Title
Cancer Res. 70
Pages: 8407-8416
Related Report
Peer Reviewed
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[Journal Article] Regulation of DNA Polymerase POLD4 Influences Genomic, Instability in Lung Cancer.2010
Author(s)
Huang QM, Tomida S, Masuda Y, Arima C, Cao K, Kasahara T, Osada H, Yatabe Y, Akashi T, Kamiya K, Takahashi T, Suzuki M
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Journal Title
Cancer Research
Volume: 70
Pages: 8407-8411
Related Report
Peer Reviewed
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[Journal Article] Translesional DNA synthesis through a C8-guanyl adduct of 2-amino-1-methyl-6- phenylimidazo[4,5-b]pyridine (PhIP) in vitro: REV1 inserts dC opposite the lesion, and DNA polymerase kappa potentially catalyzes extension resaction from the 3'-dC terminus2009
Author(s)
Fukuda H, Takamura-Enya T, Masuda Y, Nohmi T, Seki C, Kamiya K, Sugimura T, Masutani C, Hanaoka F, Nakagama H.
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Journal Title
J. Biol. Chem
Volume: 284
Pages: 2555-25592
Related Report
Peer Reviewed
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[Journal Article] DNA damage induc d ubiquity ti of C2 sub it of RFC complex.2008
Author(s)
Tomida J, Masuda Y, Hiroaki H, Ishikawa T, Song I, Tsurimoto T, Tateishi S, Shiomi T, Kamei Y, Kim J, Kamiya K, Vaziri C, Ohmori H, Todo T.
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Journal Title
J. Biol. Chem
Volume: 283
Pages: 9071-9079
Related Report
Peer Reviewed
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[Journal Article] DNA damage induced ubiquitylation of RFC2 subunit of RFC complex.2008
Author(s)
Tomida J, Masuda Y, Hiroaki H, Ishikawa T, Song I, Tsurimoto T, Tateishi S, Shiomi T, Kamei Y, Kim J, Kamiya K, Vaziri C, Ohmori H, Todo T.
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Journal Title
J.Biol.Chem. 283
Pages: 9071-9079
Related Report
Peer Reviewed
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[Journal Article] DNA damage-induced ubiquitylation of RFC2 subunit of replication factor C complex2008
Author(s)
Tomida J, Masuda Y, Hiroaki H, Ishikawa T, Song I, Tsurimoto T, Tateishi S, Shiomi T, Kamei Y, Kim J, Kamiya K, Vaziri C, Ohmori H, Todo T.
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Journal Title
Journal of Biological Chemistry 283
Pages: 9071-9079
Related Report
Peer Reviewed
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[Presentation] 放射線発がんとゲノム不安定性2011
Author(s)
笹谷めぐみ, 徐衍賓, 本田浩章, 濱崎幹也, 楠洋一郎, 渡邊敦光, 増田雄司, 神谷研二
Organizer
日本放射線影響学会第54回大会
Place of Presentation
神戸商工会議所会館(兵庫県)
Year and Date
2011-11-19
Related Report
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[Presentation] 肺癌におけるPOLD4の関与2011
Author(s)
鈴木元, 冨田秀太, 有馬千夏, 増田雄司, 神谷研二, 長田啓隆, 谷田部恭, 高橋隆
Organizer
第70回日本癌学会学術総会
Place of Presentation
名古屋国際会議場(愛知県)
Year and Date
2011-10-03
Related Report
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