Biochemical analysis of post-replication repair pathway involved in induced mutagenesis

• Project/Area Number: 20310030
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Nagoya University (2011); Hiroshima University (2008-2010)
• Principal Investigator: MASUDA Yuji 名古屋大学, 環境医学研究所, 准教授 (30273866)
• Co-Investigator(Kenkyū-buntansha): KAMIYA Kenji 広島大学, 原爆放射線医科学研究所, 教授 (60116564)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000); Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2009: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2008: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
• Keywords: 複製後修復 / 損傷乗り越え DNA 合成 / 突然変異誘発 / PCNA / RAD6-RAD18 / HLTF / 損傷乗り越えDNA合成 / ユビキチン / SHPRH / 突然変異

Research Abstract

DNA lesions produced by ionizing radiation and environmental mutagen inhibit DNA replication. Stalling replication would lead to cell death if it persisted. Cells have mechanisms (post-replication repair, PRR) to restore DNA replication to survive against DNA-damaging agents. PRR is involved in induced mutagenesis. In this study, we purifiedenzymes required for PRR, reconstituted biochemical reactions with the enzymes and analyzed molecular mechanisms of the PRR.

Research Products

• [Journal Article] In vitro studies of exchanges between replicative and translesion DNA polymerases in eukaryotic post-replication repair pathway (2012)
  • Author(s): Masuda Y
  • Journal Title: Genes Environ Volume: (in press)
  • NAID: 10030311492
  • Peer Reviewed
• [Journal Article] Molecular nature of radiation injury and DNA repair disorders associated with radiosensitivity. (2012)
  • Author(s): Masuda Y, Kamiya K
  • Journal Title: Int. J. Hematol. Volume: 95 Pages: 239-245
  • NAID: 10030551664
  • Peer Reviewed
• [Journal Article] Asymmetric nature of two subunits of RAD18, a RING-type ubiquitin ligase E3, in the human RAD6A-RAD18 ternary complex. (2012)
  • Author(s): Masuda Y, Suzuki M, Kawai H, Suzuki F, Kamiya K.
  • Journal Title: Nucleic Acids Res Volume: 40 Pages: 1065-1076
  • NAID: 120005147856
  • Peer Reviewed
• [Journal Article] Asymmetric nature of two subunits of RAD18, a RING-type ubiquitin ligase E3, in the human RAD6A-RAD18 ternary complex (2012)
  • Author(s): Masuda Y, Suzuki M, Kawai H, Suzuki F, Kamiya K
  • Journal Title: Nucleic Acids Research Volume: 40 Issue: 3 Pages: 1065-1076
  • DOI: 10.1093/nar/gkr805
  • NAID: 120005147856
  • Peer Reviewed
• [Journal Article] Molecular nature of radiation injury and DNA repair disorders associated with radiosensitivity (2012)
  • Author(s): Masuda Y, Kamiya K
  • Journal Title: International Journal of Hematology Volume: 95 Issue: 3 Pages: 239-245
  • DOI: 10.1007/s12185-012-1008-y
  • NAID: 10030551664
  • Peer Reviewed
• [Journal Article] In vitro studies of exchanges between replicative and translesion DNA nolymerases in eukaryotic post-replication repair pathway (2012)
  • Author(s): Masuda Y
  • Journal Title: Genes and Environment Volume: (未定)(掲載確定)(印刷中)
  • Peer Reviewed
• [Journal Article] Proceedings of the Ninth International Conference on Tritium Science and Technology: Development of a Sensitive Assay System for Tritium Risk Assessment Using Rev1 Transgenic Mouse. (2011)
  • Author(s): Toyoshima M, Honda H, Watanabe H, Masuda Y, Kamiya K.
  • Journal Title: Fusion Science and Technology. Volume: 60 Pages: 1204-1207
  • Peer Reviewed
• [Journal Article] The Rev1 translesion synthesis polymerase has multiple distinct DNA binding modes. (2011)
  • Author(s): de Groote FH, Jansen JG, Masuda Y, Shah DM, Kamiya K, de Wind N, Siegal G.
  • Journal Title: DNA Repair(Amst) Volume: 10 Pages: 915-25
  • Peer Reviewed
• [Journal Article] Rev1 translesion synthesis polymerase has multiple distinct DNA binding modes (2011)
  • Author(s): de Groote FH, Jansen JG, Masuda Y, Shah DM, Kamiya K, de Wind N, Siegal G
  • Journal Title: DNA Repair (Amst) Volume: 10 Issue: 9 Pages: 915-925
  • DOI: 10.1016/j.dnarep.2011.04.033
  • Peer Reviewed
• [Journal Article] Development of a Sensitive Assay System for Tritium Risk Assessment Using Rev1 Transgenic Mouse (2011)
  • Author(s): Toyoshima M, Honda H, Watanabe H, Masuda Y, Kamiya K
  • Journal Title: Fusion Science and Technology Volume: 60 Pages: 1204-1207
  • Peer Reviewed
• [Journal Article] Generation and evaluation of an anti-REV1 monoclonal antibody. (2010)
  • Author(s): Tachibana N, Iwamoto T, Kawamura T, Masuda Y, Mori T, Kamiya K.
  • Journal Title: Hiroshima J. Med. Sci. Volume: 59 Pages: 51-56
  • NAID: 120005323950
  • Peer Reviewed
• [Journal Article] Regulation of DNA polymerase POLD4 influences genomic instability in lung cancer. (2010)
  • Author(s): Huang QM, Tomida S, Masuda Y, Arima C, Cao K, Kasahara TA, Osada H, Yatabe Y, Akashi T, Kamiya K, Takahashi T, Suzuki M.
  • Journal Title: Cancer Res. Volume: 70 Pages: 8407-8416
  • Peer Reviewed
• [Journal Article] DNA replication-coupled PCNA mono-ubiquitination and polymerase switching in a human in vitrosystem. (2010)
  • Author(s): Masuda Y, Piao J, Kamiya K
  • Journal Title: J. Mol. Biol Volume: 396 Pages: 487-500
  • Peer Reviewed
• [Journal Article] Specific amino acid residues are involved in substrate discrimination and template binding of human REV1 protein. (2010)
  • Author(s): Piao J, Masuda Y, Kamiya K
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 392 Pages: 140-144
  • Peer Reviewed
• [Journal Article] Roles of POLD4, smallest subunit of DNA polymerase delta, in nuclear structures and genomic stability of human cells. (2010)
  • Author(s): Huang QM, Akashi T, Masuda Y, Kamiya K, Takahashi T, Suzuki M.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 391 Pages: 542-546
  • Peer Reviewed
• [Journal Article] Regulation of DNA polymerase POLD4 influences genomic instability in lung cancer. (2010)
  • Author(s): Huang QM, Tomida S, Masuda Y, Arima C, Cao K, Kasahara TA, Osada H, Yatabe Y, Akashi T, Kamiya K, Takahashi T, Suzuki M.
  • Journal Title: Cancer Res. 70 Pages: 8407-8416
  • Peer Reviewed
• [Journal Article] DNA replication-coupled PCNA mono-ubiquitination and polymerase switching in a human in vitro system. (2010)
  • Author(s): Masuda Y, Piao J, Kamiya K.
  • Journal Title: J.Mol.Biol 396 Pages: 487-500
  • Peer Reviewed
• [Journal Article] Specific amino acid residues are involved in substrate discrimination and template binding of human REV1 protein. (2010)
  • Author(s): Piao J, Masuda Y, Kamiya K.
  • Journal Title: Biochem. Biophys. Res. Commun. 392 Pages: 140-144
  • Peer Reviewed
• [Journal Article] Regulation of DNA Polymerase POLD4 Influences Genomic, Instability in Lung Cancer. (2010)
  • Author(s): Huang QM, Tomida S, Masuda Y, Arima C, Cao K, Kasahara T, Osada H, Yatabe Y, Akashi T, Kamiya K, Takahashi T, Suzuki M
  • Journal Title: Cancer Research Volume: 70 Pages: 8407-8411
  • Peer Reviewed
• [Journal Article] Generation and Evaluation of an Anti-REV1 Monoclonal Antibody. (2010)
  • Author(s): Tachibana N., Iwamoto T., Kawamura T., Masuda Y., Mori T., Kamiya K.
  • Journal Title: Hiroshima J Med.Sci. Volume: 59 Pages: 51-56
  • NAID: 120005323950
  • Peer Reviewed
• [Journal Article] Roles of POLD4, smallest subunit of DNA polymerase delta, in nuclear structures and genomic stability of human cells (2010)
  • Author(s): Huang QM
  • Journal Title: Biochem.Biophys.Res.Commun. 391 Pages: 542-546
  • Peer Reviewed
• [Journal Article] Specific amino acid residues are involved in substrate discrimination and template binding of human REV1 protein (2010)
  • Author(s): Piao JL
  • Journal Title: Biochem.Biophys.Res.Commun. 392 Pages: 140-144
  • Peer Reviewed
• [Journal Article] DNA replication-coupled PCNA mono-ubiquitination and polymerase swit chins in a uuman in vitro system (2010)
  • Author(s): Masuda Y
  • Journal Title: Journal of Molecular Biology 396 Pages: 487-500
  • Peer Reviewed
• [Journal Article] Translesional DNA synthesis through a C8-guanyl adduct of 2-amino-1-methyl-6- phenylimidazo[4,5-b]pyridine (PhIP) in vitro: REV1 inserts dC opposite the lesion, and DNA polymerase kappa potentially catalyzes extension resaction from the 3'-dC terminus (2009)
  • Author(s): Fukuda H, Takamura-Enya T, Masuda Y, Nohmi T, Seki C, Kamiya K, Sugimura T, Masutani C, Hanaoka F, Nakagama H.
  • Journal Title: J. Biol. Chem Volume: 284 Pages: 2555-25592
  • Peer Reviewed
• [Journal Article] Translesional DNA synthesis through a C8-guanyl adduct of 2-amino-1-methyl-6-phenylimidazo[4,5-β]pyridine(PhIP)in vitro : Rev1 inserts dC opposite the lesion and DNA polymerase κ potentially catalyzes extension reaction from the 3'-dc terminus (2009)
  • Author(s): Fukuda H
  • Journal Title: Journal of Biological Chemistry 284 Pages: 25585-25592
  • Peer Reviewed
• [Journal Article] Induced mutagenesis and translesion DNA synthesis-structure and function of REV1 (2008)
  • Author(s): Masu Y, amiya K K
  • Journal Title: Seik u Volume: 80 Pages: 843-846
• [Journal Article] Biochemical analysis of human PIF1 helicase ctions o ts N-te inal domain. (2008)
  • Author(s): Gu , agak Y Masuda Y, Kamiya K
  • Journal Title: Nucleic Acids Res Volume: 36 Pages: 6295-6308
  • Peer Reviewed
• [Journal Article] DNA damage induc d ubiquity ti of C2 sub it of RFC complex. (2008)
  • Author(s): Tomida J, Masuda Y, Hiroaki H, Ishikawa T, Song I, Tsurimoto T, Tateishi S, Shiomi T, Kamei Y, Kim J, Kamiya K, Vaziri C, Ohmori H, Todo T.
  • Journal Title: J. Biol. Chem Volume: 283 Pages: 9071-9079
  • Peer Reviewed
• [Journal Article] Biochemical analysis of human PIF1 helicase and functions of its N-terminal domain. (2008)
  • Author(s): Gu Y, Masuda Y, Kamiya K.
  • Journal Title: Nucleic Acids Res. 36 Pages: 6295-6308
  • Peer Reviewed
• [Journal Article] DNA damage induced ubiquitylation of RFC2 subunit of RFC complex. (2008)
  • Author(s): Tomida J, Masuda Y, Hiroaki H, Ishikawa T, Song I, Tsurimoto T, Tateishi S, Shiomi T, Kamei Y, Kim J, Kamiya K, Vaziri C, Ohmori H, Todo T.
  • Journal Title: J.Biol.Chem. 283 Pages: 9071-9079
  • Peer Reviewed
• [Journal Article] DNA damage-induced ubiquitylation of RFC2 subunit of replication factor C complex (2008)
  • Author(s): Tomida J, Masuda Y, Hiroaki H, Ishikawa T, Song I, Tsurimoto T, Tateishi S, Shiomi T, Kamei Y, Kim J, Kamiya K, Vaziri C, Ohmori H, Todo T.
  • Journal Title: Journal of Biological Chemistry 283 Pages: 9071-9079
  • Peer Reviewed
• [Journal Article] Biochemical analysis of human PIF1 helicase and functions of its N-terminal domain (2008)
  • Author(s): Gu Y, Masuda Y, Kamiya K.
  • Journal Title: Nucleic Acids Research 36 Pages: 6295-6308
  • Peer Reviewed
• [Journal Article] 誘発突然変異と損傷乗り越えDNA合成-REV1の構造と生化学的機能- (2008)
  • Author(s): 増田雄司, 神谷研二
  • Journal Title: 生化学 80 Pages: 843-846
  • NAID: 10022594477
• [Presentation] PCNAのユビキチン化に関わるユビキチンリガーゼ(E3)の解 (2012)
  • Author(s): 増田雄司
  • Organizer: 遺伝学研究所研究集会「ユビキチン・SUMOによる DNA 複製及び DNA 修復系の制御」
  • Place of Presentation: 立遺伝学研究所:三島(招待講演)
  • Year and Date: 2012-02-09
• [Presentation] PCNAのユビキチン化に関わるユビキチンリガーゼ(E3)の解析 (2012)
  • Author(s): 増田雄司
  • Organizer: 遺伝学研究所研究集会「ユビキチン・SUMOによるDNA複製及びDNA修復系の制御」
  • Place of Presentation: 国立遺伝学研究所(静岡県)(招待講演)
  • Year and Date: 2012-02-09
• [Presentation] D N A損傷トレランスと複製後修復経路の機能 (2011)
  • Author(s): 増田雄司、鈴木美紀、神谷研二
  • Organizer: 日本分子生物学会第34回年会
  • Place of Presentation: パシフィコ横浜:横浜(招待講演)
  • Year and Date: 2011-12-14
• [Presentation] DNA損傷トレランスと複製後修復経路の機能 (2011)
  • Author(s): 増田雄司, 鈴木美紀, 神谷研二
  • Organizer: 日本分子生物学会第34回年会
  • Place of Presentation: パシフィコ横浜(神奈川県)(招待講演)
  • Year and Date: 2011-12-14
• [Presentation] 誘発突然変異とその制御機構-PCNAのユビキチン修飾の分子機構の解析から- (2011)
  • Author(s): 増田雄司
  • Organizer: 名古屋大学環境医学研究所
  • Place of Presentation: 群馬大学 昭和キパス ャン :前橋(招待講演)
  • Year and Date: 2011-12-09
• [Presentation] 誘発突然変異とその制御機構-PCNAのユビキチン修飾の分子機構の解析から- (2011)
  • Author(s): 増田雄司
  • Organizer: 名古屋大学環境医学研究所、群馬大学生体調節研究所、第八回合同シンポジウム
  • Place of Presentation: 群馬大学昭和キャンパス(群馬県)(招待講演)
  • Year and Date: 2011-12-09
• [Presentation] 放射線によるDNA損傷と複製後修復経路の機能 (2011)
  • Author(s): 増田雄司、鈴木美紀、神谷研二
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸商工会議所会館:神戸(招待講演)
  • Year and Date: 2011-11-19
• [Presentation] γ線の照射環境下での細胞応答の線量率依存性の解析 (2011)
  • Author(s): 河合秀彦, 曹麗麗, 飯塚大輔, 松井啓隆, 金井昭教, 稲葉俊哉, 増田雄司, 笹谷めぐみ, 神谷研二, 鈴木文男
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸商工会議所会館(兵庫県)
  • Year and Date: 2011-11-19
• [Presentation] 放射線発がんとゲノム不安定性 (2011)
  • Author(s): 笹谷めぐみ, 徐衍賓, 本田浩章, 濱崎幹也, 楠洋一郎, 渡邊敦光, 増田雄司, 神谷研二
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸商工会議所会館(兵庫県)
  • Year and Date: 2011-11-19
• [Presentation] PCNAユビキチン化酵素RAD6-RAD18複合体の解析 (2011)
  • Author(s): 増田雄司, 鈴木美紀, 神谷研二
  • Organizer: 第21回DNA複製・組換え・ゲノム安定性制御ワークショップ
  • Place of Presentation: サンビア福岡(福岡県)
  • Year and Date: 2011-10-25
• [Presentation] 損傷DNAの複製とPCNAのポリユビキチン化の分子機構 (2011)
  • Author(s): 増田雄司, 神谷研二
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2011-10-05
• [Presentation] Rev1の過剰発現が発がんに及ぼす役割 (2011)
  • Author(s): 笹谷めぐみ, 本田浩章, 増田雄司, 渡邊敦光, 神谷研二
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2011-10-05
• [Presentation] 肺癌におけるPOLD4の関与 (2011)
  • Author(s): 鈴木元, 冨田秀太, 有馬千夏, 増田雄司, 神谷研二, 長田啓隆, 谷田部恭, 高橋隆
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2011-10-03
• [Presentation] ヒトPCNAのユビキチン化酵素RAD6-RAD18複合体の解析 (2011)
  • Author(s): 増田雄司, 鈴木美紀, 神谷研二
  • Organizer: 日本遺伝学会第83回大会
  • Place of Presentation: 京都大学(京都府)
  • Year and Date: 2011-09-21
• [Presentation] The role of Rev1 in radiation and chemical-induced tumor development using Rev1 Tg mice (2011)
  • Author(s): M Sasatani, H Honda, K Hamasaki, Y Kusunoki, Y Masuda, K Kamiya
  • Organizer: 14th International Congress of Radiation Research
  • Place of Presentation: The Palace of Culture and Science (Warsaw, Poland)
  • Year and Date: 2011-09-01
• [Presentation] DNA damage tolerance and molecular mechanism of PCNA ubiquitination (2011)
  • Author(s): Masuda Y, Suzuki M, Kamiya K
  • Organizer: 14th International Congress of Radiation Research
  • Place of Presentation: Warsaw, Poland
  • Year and Date: 2011-08-30
• [Presentation] DNA damage tolerance and molecular mechanism of PCNA ubiquitination (2011)
  • Author(s): Y Masuda, M Suzuki, K Kamiya
  • Organizer: 14th International Congress of Radiation Research
  • Place of Presentation: The Palace of Culture and Science (Warsaw, Poland)
  • Year and Date: 2011-08-30
• [Presentation] Apc^<Min/+>マウスを用いた放射線誘発消化管腫瘍の解析 (2011)
  • Author(s): 笹谷めぐみ, 徐衍ヘン, 本田浩章, 楠洋一郎, 渡邊敦光, 増田雄司, 神谷研二
  • Organizer: 第36回中国地区放射線影響研究会
  • Place of Presentation: 広島大学霞キャンパス(広島県)
  • Year and Date: 2011-07-28
• [Presentation] 複製後修復経路におけるPCNAのポリユビキチン化の機能解析 (2011)
  • Author(s): 鈴木美紀, 増田雄司, 神谷研二
  • Organizer: 第36回中国地区放射線影響研究会
  • Place of Presentation: 広島大学霞キャンパス(広島県)
  • Year and Date: 2011-07-28
• [Presentation] 複製後修復経路におけるユビキチンリガーゼRAD18の機能解析 (2011)
  • Author(s): 増田雄司, 鈴木美紀, 神谷研二
  • Organizer: 第36回中国地区放射線影響研究会
  • Place of Presentation: 広島大学霞キャンパス(広島県)
  • Year and Date: 2011-07-28
• [Presentation] RAD6-RAD18複合体の構造と機能 (2011)
  • Author(s): 増田雄司, 鈴木美紀, 神谷研二
  • Organizer: 日本環境変異原学会-変異機構研究会・第24回夏の学校
  • Place of Presentation: 小牧勤労センター(愛知県)
  • Year and Date: 2011-07-09
• [Presentation] 放射線によって誘発されるDNA損傷に対する複製後修復経路の機能解析 (2011)
  • Author(s): 増田雄司, 笹谷めぐみ, 神谷研二
  • Organizer: 第52回原子爆弾後障害研究会
  • Place of Presentation: 広島国際会議場(広島県)
  • Year and Date: 2011-06-05
• [Presentation] Apc<Min/+>マウスにおける放射線誘発消化管腫瘍の解析 (2011)
  • Author(s): 笹谷めぐみ, 増田雄司, 神谷研二
  • Organizer: 第52回原子爆弾後障害研究会
  • Place of Presentation: 広島国際会議場(広島県)
  • Year and Date: 2011-06-05
• [Presentation] 突然変異誘発経路の制御機構～PCNAのユビキチン修飾の生化学的解析から (2011)
  • Author(s): 増田雄
  • Organizer: 平成 23 年度 日本環境変異原学会公開シンポジウム
  • Place of Presentation: 慶應義塾大 共立キャンパス:東京(招待講演)
  • Year and Date: 2011-05-28
• [Presentation] 突然変異誘発経路の制御機構～PCNAのユビキチン修飾の生化学的解析から (2011)
  • Author(s): 増田雄司
  • Organizer: 平成23年度日本環境変異原学会公開シンポジウム
  • Place of Presentation: 慶應義塾大学芝共立キャンパス(東京都)(招待講演)
  • Year and Date: 2011-05-28
• [Presentation] Molecular mechanisms of PCNA ubiquitination (2011)
  • Author(s): Yuji Masuda, Miki Suzuki, Hidehiko Kawai, Asami Hishiki, Hiroshi Hashimoto, Fumio Suzuki, Kenji Kamiya
  • Organizer: The 1st RIRBM International Symposium
  • Place of Presentation: 広島市
  • Year and Date: 2011-03-03
• [Presentation] 放射線によるDNA損傷と複製後修復経路の機能 (2011)
  • Author(s): 増田雄司, 鈴木美紀, 神谷研二
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸商工会議所会館(兵庫県)(招待講演)
• [Presentation] Biochemical analysis of PCNA-ubiquitination in humans (2010)
  • Author(s): Masuda Y, Suzuki M, Kawai H, Hishiki A, Hashimoto H, uzuki F, S ami K ya K
  • Organizer: The 7th 3R (Replication, Recombination and Repair) Symposium
  • Place of Presentation: 富山
  • Year and Date: 2010-10-26
• [Presentation] Biochemical analysis of PCNA-ubiquitination in humans (2010)
  • Author(s): Yuji Masuda, Miki Suzuki, Hidehiko Kawai, Asami Hishiki, Hiroshi Hashimoto, Fumio Suzuki, Kenji Kamiya
  • Organizer: The 7th 3R (Replication, Recombination and Repair) Symposium
  • Place of Presentation: 富山市
  • Year and Date: 2010-10-26
• [Presentation] P C N Aのユビキチン化反応の生化学的解析 (2010)
  • Author(s): 増田雄司、鈴木美紀、可合秀彦、菱木麻美、橋本 博、鈴木文男、神谷研二
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-20
• [Presentation] PCNAのユビキチン化反応の生化学的解析 (2010)
  • Author(s): 増田雄司、鈴木美紀、可合秀彦、菱木麻美、橋本博、鈴木文男、神谷研二
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都市
  • Year and Date: 2010-10-20
• [Presentation] 試験管内のPCNAのユビキチン化反応系の構築 (2010)
  • Author(s): 鈴木美紀、増田雄司、神谷研二
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都市
  • Year and Date: 2010-10-20
• [Presentation] 放射線発がんにおける損傷乗り越えDNA合成酵素Rev1の及ぼす寄与 (2010)
  • Author(s): 笹谷めぐみ、本田浩章、濱崎幹也、楠洋一郎、渡邊敦光、増田雄司、神谷研二
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都市
  • Year and Date: 2010-10-20
• [Presentation] ヒトPCNAのモノユビキチン化とDNAポリメラーゼ交換反応の生化学的解析 (2010)
  • Author(s): 増田雄司、神谷研二
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪市
  • Year and Date: 2010-09-24
• [Presentation] Rev1の過剰発現は化学物質による発がんを促進する (2010)
  • Author(s): 笹谷めぐみ、本田浩章、増田雄司、渡邊敦光、神谷研二
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪市
  • Year and Date: 2010-09-24
• [Presentation] 肺小細胞癌に見られたPOLD4低発現 (2010)
  • Author(s): 鈴木元、冨田秀太、有馬千夏、増田雄司、神谷研二、長田啓隆、谷田部恭、黄勤びょう、高橋隆
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪市
  • Year and Date: 2010-09-24
• [Presentation] PCNAのユビキチン化の分子機構 (2010)
  • Author(s): 増田雄司、鈴木美紀、河合秀彦、菱木麻美、橋本博、鈴木文男、神谷研二
  • Organizer: 日本遺伝学会第82回大会
  • Place of Presentation: 札幌市
  • Year and Date: 2010-09-23
• [Presentation] Dynamics of human replicatio f ctors and DNA olym rase switching (2009)
  • Author(s): Masuda Y
  • Organizer: Mini-Workshop on "DNA Polymerase"
  • Place of Presentation: 奈良先端科学技 大学院・大学:生駒(招待講演)
  • Year and Date: 2009-11-27
• [Presentation] Dynamics of human replication factors and DNA polymerase switching (2009)
  • Author(s): 増田雄司
  • Organizer: Mini-Workshop on "DNA Polymerase"
  • Place of Presentation: 奈良先端科学技術大学院大学
  • Year and Date: 2009-11-27
• [Presentation] 放射線発がん、化学発がんにおける損傷乗り越えDNA合成酵素Rev1の役割 (2009)
  • Author(s): 豊島めぐみ
  • Organizer: 日本放射線影響学会第52回大会
  • Place of Presentation: 広島市
  • Year and Date: 2009-11-13
• [Presentation] 複製後修復経路におけるDNAポリメラーゼ交換反応の生化学的解析 (2009)
  • Author(s): 増田雄司、神谷研二
  • Organizer: 日本放射線影響学会第52回大会
  • Place of Presentation: 広島
  • Year and Date: 2009-11-12
• [Presentation] 複製後修復経路におけるDNAポリメラーゼ交換反応の生化学的解析 (2009)
  • Author(s): 増田雄司、神谷研二
  • Organizer: 日本放射線影響学会第52回大会
  • Place of Presentation: 広島市
  • Year and Date: 2009-11-12
• [Presentation] 損傷乗り越えDNA合成酵素Rev1の放射線応答に対する影響 (2009)
  • Author(s): 三家本隆宏
  • Organizer: 日本放射線影響学会第52回大会
  • Place of Presentation: 広島市
  • Year and Date: 2009-11-12
• [Presentation] Gene profiling identified a gene involved in genetic instability in lung cancer (2009)
  • Author(s): Suzuki M
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-10-03
• [Presentation] Biochemical analysis of PCNA mono-ubiquitination with human RAD6-RAD18 (2009)
  • Author(s): 増田雄司
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-10-01
• [Presentation] Over-expression of Rev1 acceraltes N-methyl-N-nitrosourea-induced tumorigenesisi in mice (2009)
  • Author(s): 豊島めぐみ
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-10-01
• [Presentation] DNA-damage Checkpoint Response to PhlP-exposure and Translesion DNA Synthesis at PhlP-dG (2009)
  • Author(s): Fukuda H
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-10-01
• [Presentation] 損傷乗り越えDNA合成におけるポリメラーゼ交換反応の生化学的解析 (2009)
  • Author(s): 増田雄司
  • Organizer: 日本遺伝学会第81回大会
  • Place of Presentation: 松本市
  • Year and Date: 2009-09-16
• [Presentation] Reconstitution of DNA replication reactions with purified recombinant human proteins in vitro (2009)
  • Author(s): Masuda Y, Suzuki M, Piao, J, Gu Y, Tsurumoto T, Kamiya K
  • Organizer: 2nd Asian Congress of Radiation Research
  • Place of Presentation: Korea
  • Year and Date: 2009-05-17
• [Presentation] Reconstitution of DNA replication reactions with purified recombinant human proteins in vitro (2009)
  • Author(s): 増田雄司
  • Organizer: 2nd Asian Congress of Radiation Research (ACRR 2009)
  • Place of Presentation: Seoul, the Republic of Korea
  • Year and Date: 2009-05-17
• [Presentation] Function of Rev1 on Carcinogenesis induced by ionizing radiation and chemical agents (2009)
  • Author(s): 豊島めぐみ
  • Organizer: 2nd Asian Congress of Radiation Research (ACRR 2009)
  • Place of Presentation: Seoul, the Republic of Korea
  • Year and Date: 2009-05-17
• [Presentation] ヒトRAD6-RAD18 によるPCNAのユビキチン化反応の分子機構 (2008)
  • Author(s): 増田雄司、神谷研二
  • Organizer: 日本放射線影響学会第51回大会
  • Place of Presentation: 北九州
  • Year and Date: 2008-11-19
• [Presentation] ヒトRAD6-RAD18によるPCNAのユビキチン化反応の分子機構 (2008)
  • Author(s): 増田雄司、神谷研二
  • Organizer: 日本放射線影響学会第51回大会
  • Place of Presentation: 北九州市
  • Year and Date: 2008-11-19
• [Presentation] 損傷乗り越えDNA合成酵素Revlの放射線応答、放射線発がんにおける寄与 (2008)
  • Author(s): 豊島めぐみ, 習 陽, 本田浩章, 濱崎幹也, 楠 洋一郎, 渡邊敦光, 増田雄司, 神谷研二
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 北九州市
  • Year and Date: 2008-11-19
• [Presentation] ヒトREV1の鋳型への結合と基質の識別に関与するアミノ酸残基の解析 (2008)
  • Author(s): 増田雄司, 朴金蓮, 神谷研二
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2008-10-28
• [Presentation] 発がんにおける損傷乗り越えDNA合成酵素Revlの役割 (2008)
  • Author(s): 豊島めぐみ, 本田浩章, 増田雄司, 渡辺敦光, 柿沼志津子, 島田義也, 神谷研二
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2008-10-28
• [Presentation] ヒトRAD6-RAD18によるPCNAのモノュビキチン化反応の解析 (2008)
  • Author(s): 増田雄司, 神谷研二
  • Organizer: 日本遺伝学会第80回大会
  • Place of Presentation: 名古屋市
  • Year and Date: 2008-09-03
• [Remarks]
  • URL: http://www.riem.nagoya-u.ac.jp/genome.html
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/exponc/inhtml
• [Remarks]
  • URL: http://www.riem.nagoya-u.ac.jp/