A highly sensitive assay system for examining chemical mutagenesity and carcinogenesity using DNA repair-deficient mice
| Project/Area Number |
20310031
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Kyushu University |
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Principal Investigator |
TSUZUKI Teruhisa Kyushu University, 大学院・医学研究院, 教授 (40155429)
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| Co-Investigator(Kenkyū-buntansha) |
NAKATSU Yoshimichi 九州大学, 大学院・医学研究院, 准教授 (00207820)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2008: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
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| Keywords | 遺伝子 / 核酸 / 環境 / ゲノム / 放射線 / 突然変異 / 酸化ストレス / 活性酸素 |
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| Research Abstract |
In order to establish a highly sensitive assay system for examining chemical mutagenesity and tumorigenesity, we performed KBrO_3-induced mutagenesis and tumorigenesis experiments using Ogg1-, Mutyh- or Msh2-deficient mice. We observed an enhanced tumor-formation in the small intestines of Mutyh- or Msh2-deficient mice, as compared with wild type and heterozygous mice. These results indicate that Mutyh- or Msh2-deficient mice are ideal animal models for examining tumorigenesity of chemicals of interest. The number of tumors was marginally increased in Ogg1-deficient mice in which the increased KBrO_3-induced mutagenesis was observed at an almost same extent as in Mutyh-deficient mice, suggesting that the induction of cell death might play a more important role than the suppression of mutagenesis for avoiding intestinal tumorigenesis caused by oxidative stress.
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Report
(4 results)
Research Products
(100 results)
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[Journal Article] Comprehensive behavioral study of GluR4 knockout mice : implication in cognitive function.2010
Author(s)
N.Sagata, A.Iwaki, T.Aramaki, K.Takao, S.Kura, T.Tsuzuki, R.Kawakami, I.Ito, T.Kitamura, H.Sugiyama, T.Miyakawa, Y.Fukumaki
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Journal Title
Genes, Brain Behavior 9
Pages: 899-909
Related Report
Peer Reviewed
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[Journal Article] Structural and dynamic features of the MutT protein in the recognition of nucleotides with the mutagenic 8-oxoguanine base2010
Author(s)
T.Nakamura, S.Meshitsuka, S.Kitagawa, N.Abe, J.Yamada, T.Ishino, H.Nakano, T.Tsuzuki, T.Doi, Y.Kobayashi, S.Fujii, M.Sekiguchi, Y.Yamagata
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Journal Title
J.Biol.Chem. 285
Pages: 444-452
Related Report
Peer Reviewed
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[Presentation] 哺乳類細胞のゲノム安定性と細胞死2010
Author(s)
日高真純, 高木康光, Teik-How Lim, 中津可道, 續輝久, 佐野しおり, 坂上竜資, 関口睦夫
Organizer
日本遺伝学会第82回大会
Place of Presentation
札幌
Year and Date
2010-09-20
Related Report
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