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Development of phytoremediation system for removal of persistent phenolic pollutants

Research Project

Project/Area Number 20310043
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental technology/Environmental materials
Research InstitutionOsaka University

Principal Investigator

HIRATA Kazumasa  Osaka University, 大学院・薬学研究科, 教授 (30199062)

Co-Investigator(Kenkyū-buntansha) NAKAYAMA Hideki  奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (30324982)
KATO Ko  奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (80283935)
HARADA Kazuo  大阪大学, 大学院・薬学研究科, 助教 (50397741)
Co-Investigator(Renkei-kenkyūsha) MIYASAKA Hitoshi  関西電力株式会社環境技術研究センター, チーフリサーチャー (60451283)
BAMBA Takeshi  大阪大学, 大学院・工学研究科, 准教授 (10432444)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2009: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2008: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywords環境浄化 / 内分泌かく乱物質 / ペルオキシダーゼ / ポリフェノールオキシダーゼ / 遺伝子組換え植物
Research Abstract

In this study, it was uncovered the following things. i) The key enzymes which detoxicate phenolic endocrine-disrupting chemicals in Portulaca oleracea, that is a promising plant in phytoremediation, are 'polyphenol oxidase' and 'peroxidase'. ii) DNA sequences of these enzymes were decided. iii) These enzymes immobilized onto alginate and glass beads can detoxicate phenolic endocrine-disrupting chemicals under the conditions in which any plant was not able to grow. iv) Salvia cultivars showed a high BPA-eliminating ability under low temperature.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (11 results)

All 2011 2010 2009

All Journal Article (7 results) (of which Peer Reviewed: 5 results) Presentation (4 results)

  • [Journal Article] Molecular cloning and partial characterization of a peroxidases gene expressed in roots of Portulaca oleracea cv. that is potentially useful for the remediation of phenolic pollutants2011

    • Author(s)
      Takeshi Matsui, Yuki Nomura, Mai Takano, Sofue Imai, Hideki Nakayama, Hitoshi Miyasaka, Hitoshi Okuhata, Satoshi Tanaka, Hideyuki Matsuura, Kazuo Harada, Takeshi Bamba, Kazumasa Hirata, Ko Kato
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry in press

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Cloning and Partial Characterization of a Peroxidase Gene Expressed in Roots of Portulaca oleracea cv.that is Potentially Useful for the Remediation of Phenolic Pollutants.2011

    • Author(s)
      Takeshi Matsui, Yuki Nomura, Mai Takano, Sofue Imai, Hideki NakayamaA, Hitoshi Miyasaka, Hiroshi Okuhata, Satoshi Tanaka, Hideyuki Matsuura, Kazuo Harada, Takeshi Bamba, Kazumasa Hirata, Ko Kato
    • Journal Title

      Biosci.Biotech.Biochem.

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Floricultural Salvia plants have a high ability to eliminate bisphenol A2010

    • Author(s)
      Hiroshi Okuhata, Kazunori Ikeda, Hitoshi Miyasaka, Satoru Takahashi, Takeshi Matsui, Hideki Nakayama, Ko Kato, Kazumasa Hirata
    • Journal Title

      Journal of Bioscience and Bioengineering 110

    • NAID

      110007681452

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Floricultural Salvia plants have a high ability to eliminate disphenol A.2010

    • Author(s)
      Okuhata; H., Ikeda; K., Miyasaka; H., Takahashi; S., Matsui; T., Nakayama; H., Kato; K., Hirata, K.
    • Journal Title

      Journal of Bioscience and Bioengineering 109(In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 植物の多様を代謝能を利用した内分泌撹乱物質除去技術の開発2010

    • Author(s)
      平田収正
    • Journal Title

      生産と技術 62(In press)

    • Related Report
      2009 Annual Research Report
  • [Journal Article] 環境ホルモンを分解する植物-環境浄花-2009

    • Author(s)
      平田収正、原田和生、奥畑博史、宮坂均
    • Journal Title

      Bio Industry 11

      Pages: 15-20

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 環境ホルモンを分解する植物2009

    • Author(s)
      平田収正, 原田和生, 奥畑博史, 宮坂均
    • Journal Title

      バイオインダストリー 26

      Pages: 15-20

    • Related Report
      2009 Annual Research Report
  • [Presentation] 園芸植物Portulaca oleracea由来ポリフェノールオキシダーゼの遺伝子単離と内分泌撹乱物質分解能の解析2010

    • Author(s)
      金田洋和, 松井健史, 富安諒介, 渡辺一平, 黒田友佳子, 松浦秀幸, 原田和生, 奥畑博史, 仲山英樹, 宮坂均, 加藤晃, 平田牧正
    • Organizer
      第28回日本植物細胞分子生物学会(仙台)大会
    • Place of Presentation
      東北大学
    • Year and Date
      2010-09-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] 園芸植物Portulaca oleracea 由来ポリフェノールオキシダーゼの遺伝子単離と内分泌撹乱物質代謝能の解析2010

    • Author(s)
      金田洋和、松井健史、富安諒介、渡辺一平、黒田友佳子、松浦秀幸、原田和生、奥畑博史、仲山英樹、宮坂均、加藤晃、平田収正
    • Organizer
      第28回日本植物細胞分子生物学会
    • Place of Presentation
      東北大学
    • Year and Date
      2010-09-02
    • Related Report
      2010 Final Research Report
  • [Presentation] 園芸植物Portulaca oleracea由来BPA代謝酵素の固定化2010

    • Author(s)
      金田洋和
    • Organizer
      平成22年度生物工学若手研究者の集い
    • Place of Presentation
      岡山県倉敷シーサイドホテル
    • Year and Date
      2010-07-03
    • Related Report
      2010 Final Research Report
  • [Presentation] Portulaca oleraceaによる内分泌撹乱物質の浄化2009

    • Author(s)
      黒田友佳子
    • Organizer
      第4回質量分析夏の学校
    • Place of Presentation
      大阪府長居ユースホステル
    • Year and Date
      2009-09-18
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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