Establishment of the all biosynthetic system for new monomers-contained biopolymers as a basis for enzyme evolutionary engineering
| Project/Area Number |
20310050
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Hokkaido University |
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Principal Investigator |
TAGUCHI Seiichi Hokkaido University, 大学院・工学研究院, 教授 (70216828)
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| Co-Investigator(Kenkyū-buntansha) |
SATOH Toshifumi 北海道大学, 大学院・工学研究院, 准教授 (80291235)
MATSUMOTO Ken'ichiro 北海道大学, 大学院・工学研究院, 助教 (80360642)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2009: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2008: ¥11,960,000 (Direct Cost: ¥9,200,000、Indirect Cost: ¥2,760,000)
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| Keywords | 循環材料生産システム / 生分解性プラスチック / バイオプロセス / 進化工学 / ポリヒドロキシアルカン酸 / バイオベースポリマー / 乳酸ポリマー / 微生物工場 / 重合酵素 / キラルモノマー / 基質特異性 / 進化分子工学 / ポリマー物性 / モノマー組成 |
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| Research Abstract |
Microbial production system for lactate (LA) polymers with high performances has been established. This new microbial factory can be driven by renewable biomass resources. A break-through of this system was a discovery of "LA-Polymerizing Enzyme (LPE)". LPE was created through the study on the engineering of microbial polymer PHA synthase. LA-based polymer was synthesized as an one-pot manner in Escherichia coli by introduction of the LPE gene from glucose. Also, LA fraction in the polymer has been enriched up to 47% from 6% by combination of metabolic and enzyme engineering. Furthermore, LA-based polymers exhibited distinguishable properties from the counterpart homopolymers, PLA and PHB.
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Report
(4 results)
Research Products
(69 results)
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[Journal Article] A microbial factory for lactate-based polyesters using a lactate-polymerizing enzyme2008
Author(s)
S.Taguchi, M.Yamada, K.Matsumoto, K.Tajima, Y.Sato, M.Munekata, K.Ohono, K.Kohda, T.Shimamura, H.Kambe, S.Obata
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Journal Title
Proc.Natl.Acad.Sci.USA 105
Pages: 17323-17327
Related Report
Peer Reviewed
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