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Development of Microdevices for Cell Assay

Research Project

Project/Area Number 20310072
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Microdevices/Nanodevices
Research InstitutionNagoya University

Principal Investigator

TOKESHI Manabu  Nagoya University, 工学研究科, 准教授 (60311437)

Co-Investigator(Renkei-kenkyūsha) BABA Yoshinobu  名古屋大学, 大学院・工学研究科, 教授 (30183916)
KAJI Noritada  名古屋大学, 大学院・工学研究科, 助教 (90402479)
CHAN Yu  名古屋大学, 大学院・工学研究科, 研究員 (40467329)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2008: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Keywordsマイクロ化学チップ / 細胞アッセイ / リアルタイムPCR
Research Abstract

We developed a micro device that a series of bioassay from the cell culture to the gene expression analysis is possible. Two kinds of devices were developed : a device that enables circumfusion culture of suspension cells (yeast) while exchanging media, and a genetic analysis device that enables real-time PCR. An integrated device of these two devices was successfully developed.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (58 results)

All 2011 2010 2009 Other

All Journal Article (16 results) (of which Peer Reviewed: 13 results) Presentation (41 results) Remarks (1 results)

  • [Journal Article] 量子ドットによる幹細胞のin vivoイメージング2011

    • Author(s)
      渡辺将生, 他8人
    • Journal Title

      生命化学研究レター

      Volume: 35 Pages: 38-42

    • Related Report
      2010 Annual Research Report
  • [Journal Article] 電気学会論文誌E2010

    • Author(s)
      杉浦佳奈子、加地範匡、岡本行広、渡慶次学、馬場嘉信
    • Journal Title

      130巻

      Pages: 471-475

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article]2010

    • Author(s)
      Hiroshi Yukawa, Yukimasa Kagami, Masaki Watanabe, Koichi Oishi, Yoshitake Miyamoto, Yukihiro Okamoto, Manabu Tokeshi Noritada Kaji, Hirofumi Noguchi, Kenji Ono, Makoto Sawada, Yoshinobu Baba, Nobuyuki Hamajima, Shuji Hayashi
    • Journal Title

      Biomaterials 31

      Pages: 4094-4103

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 培養チップとアッセイチップを組み合わせた簡便なハイスループット細胞アッセイシステムの開発2010

    • Author(s)
      杉浦佳奈子, 他4人
    • Journal Title

      電気学会論文誌E

      Volume: 10 Pages: 471-475

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantum Dots Labeling Using Octa-Arginine Peptides for Imaging of A dipose Tissue-Derived Stem Cell2010

    • Author(s)
      Hiroshi Yukawa, 他13人
    • Journal Title

      Biometerials

      Volume: 31 Pages: 4094-4103

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 量子ドットによる細胞移植後の幹細胞イメージング2010

    • Author(s)
      渡辺将生, 他8人
    • Journal Title

      ナノ学会会報

      Volume: 9 Pages: 7-12

    • Related Report
      2010 Annual Research Report
  • [Journal Article]2009

    • Author(s)
      Kanako Sugiura, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba
    • Journal Title

      Proc.μ-TAS 2009 2

      Pages: 1243-1245

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article]2009

    • Author(s)
      Toyohiro Naito, Ai Yatsuhashi, Noritada Kaji, Taeko Ando, Kazuo Sato, Hisao Moriya, Hiroaki Kitano, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba
    • Journal Title

      Paoc.μ-TAS 2009 1

      Pages: 627-629

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] On-Chip Real Time PCR for Genetic Tug-of-War (g-TOW) Experiment2009

    • Author(s)
      Toyohiro Naito, 他9人
    • Journal Title

      Proceedings of μTAS 2009

      Volume: 1 Pages: 627-629

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Easy Cell Assay Device for Two Microchannels2009

    • Author(s)
      Kanako Sugiura, 他4人
    • Journal Title

      Proceedings of μTAS 2009

      Volume: 2 Pages: 1243-1245

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantum Dots for Labeling Adipose Tissue-Derived Stem Cells2009

    • Author(s)
      Hiroshi Yukawa, 他12人
    • Journal Title

      Cell Transplantation

      Volume: 18 Pages: 591-599

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 量子ドットを用いた脂肪組織由来幹細胞のバイオイメージング2009

    • Author(s)
      鏡味幸真, 他7人
    • Journal Title

      バイオインダストリー

      Volume: 12 Pages: 14-18

    • Related Report
      2009 Annual Research Report
  • [Journal Article] On-Chip Real Time PCR for Genetic Tug-of-War(g-TOW)Experiment2009

    • Author(s)
      Toyohiro Naito, 他9人
    • Journal Title

      Proceedings of μTAS 2009 1

      Pages: 627-629

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Easy Cell Assay Device for Two Microchannels2009

    • Author(s)
      Kanako Sugiura, 他4人
    • Journal Title

      Proceedings of μTAS 2009 2

      Pages: 1243-1245

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantum Dots for Labeling Adipose Tissue-Derived Stem Cells2009

    • Author(s)
      Hiroshi Yukawa, 他12人
    • Journal Title

      Cell Transplantation 18

      Pages: 591-599

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 量子ドットを用いた脂肪組織由来幹細胞のバイオイメージング2009

    • Author(s)
      鏡味幸真, 他7人
    • Journal Title

      バイオインダストリー 12

      Pages: 14-18

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation]2011

    • Author(s)
      内藤豊裕、加地範匡、岡本行広、渡慶次学、馬場嘉信
    • Organizer
      日本化学会第91春季年会(要旨集)
    • Year and Date
      2011-03-11
    • Related Report
      2010 Final Research Report
  • [Presentation] マイクロ化学チップの高集積化に向けた熱応答性バルブの開発2011

    • Author(s)
      内藤豊裕, 他8人
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      要旨集
    • Year and Date
      2011-03-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] 量子ドットを用いた移植幹細胞のin vivoイメージング2011

    • Author(s)
      渡辺将生, 他8人
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      要旨集
    • Year and Date
      2011-03-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] International Symposium : Advanced Science and Technology for Single Molecular Analysis of DNA and Related Molecules(ISSMA 2011)2011

    • Author(s)
      Toyohiro Naito, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba
    • Place of Presentation
      Kyoto, Japan.
    • Year and Date
      2011-01-24
    • Related Report
      2010 Final Research Report
  • [Presentation] A Simple Structured Microvalve Actuated by Heating2011

    • Author(s)
      Toyohiro Naito, 他4人
    • Organizer
      International Symposium : Advanced Science and Technology for Single Molecular Analysis of DNA and Related Molecules (ISSMA 2011)
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2011-01-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] Microfluidic Devices for Medical Diagnostics2010

    • Author(s)
      Manabu Tokeshi
    • Organizer
      The International Chemical Congress of Pacific Basin Societies (Pacifichem 2010)
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      2010-12-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] The International Chemical Congress of Pacific Basin Societies(Pacifichem 2010)2010

    • Author(s)
      Toyohiro Naito, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba
    • Place of Presentation
      Hawaii, USA.
    • Year and Date
      2010-12-16
    • Related Report
      2010 Final Research Report
  • [Presentation] Microfluidic Device with Tilted Micropost Array for Cell Concentration2010

    • Author(s)
      Toyohiro Naito, 他4人
    • Organizer
      The International Chemical Congress of Pacific Basin Societies (Pacifichem 2010)
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      2010-12-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] Quantum Dot Based Imaging of Adipose-Derived Stem Cells for Stem Cell Therapy2010

    • Author(s)
      Masaki Watanabe, 他4人
    • Organizer
      The International Chemical Congress of Pacific Basin Societies (Pacifichem 2010)
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      2010-12-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] マイクロバイオチップ2010

    • Author(s)
      渡慶次学
    • Organizer
      未踏・ナノデバイステクノロジー第151委員会研究会ナノバイオフユージョン分科会合同研究会
    • Place of Presentation
      早稲田大学
    • Year and Date
      2010-12-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] Applications of Labb-on-a-Chip Devices in Biotechnology, Medicine and Clinical Diagnosis2010

    • Author(s)
      Manabu Tokeshi
    • Organizer
      23rd International Microprocesses and Nanotechnology Conference (MNC2010)
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2010-11-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] バイオ燃料生成に向けたマイクロ細胞ハンドリングデバイスの開発2010

    • Author(s)
      内藤豊裕, 他8人
    • Organizer
      名古屋大学材料バックキャストテクノロジーシンポジウム
    • Place of Presentation
      名古屋大学野依記念学術交流館
    • Year and Date
      2010-09-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] 硬化性樹脂を用いたダイヤフラム型ニューマティックバルブの作製法2010

    • Author(s)
      内藤豊裕, 他7人
    • Organizer
      第21回化学とマイクロ・ナノシステム研究会
    • Place of Presentation
      東京大学小柴ホール
    • Year and Date
      2010-06-11
    • Related Report
      2010 Annual Research Report
  • [Presentation] 量子ドットによる細胞移植後の幹細胞イメージング2010

    • Author(s)
      渡辺将生, 他8人
    • Organizer
      ナノ学会第8回大会
    • Place of Presentation
      岡崎コンファレンスセンター
    • Year and Date
      2010-05-13
    • Related Report
      2010 Annual Research Report
  • [Presentation] 脂肪組織由来幹細胞の生体内イメージングに向けた量子ドットの応用2010

    • Author(s)
      鏡味幸真, 他7人
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      近畿大学本部キャンパス
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report 2008 Annual Research Report
  • [Presentation] 量子ドットを用いた脂肪組織由来幹細胞のin vivoイメージング2010

    • Author(s)
      渡辺将生, 他7人
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      近畿大学本部キャンパス
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] 細胞濃縮のためのマイクロ流体デバイスの開発2010

    • Author(s)
      内藤豊裕, 他4人
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      近畿大学本部キャンパス
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report 2008 Annual Research Report
  • [Presentation] 量子ドットを用いた脂肪組織由来幹細胞の in vivo イメージング2010

    • Author(s)
      渡辺将生, 他7人
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      近畿大学本部キャンパス
    • Year and Date
      2010-03-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] In Vivo Imaging of Stem Cells Using Quantum Dots for Stem Cell Therapy2010

    • Author(s)
      Masaki Watanabe, 他9人
    • Organizer
      Lorentz Center Workshop Micro- and Nanofluidics for Cell Biology
    • Place of Presentation
      Leiden, Netherland
    • Year and Date
      2010-01-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] In Vivo Imaging of Stem Cells Using Quantum Dots for Stem Cell Therapy2010

    • Author(s)
      渡辺将生, 他9人
    • Organizer
      Lorentz Center Workshop Micro-and Nanofluidics for Cell Biology
    • Place of Presentation
      Leiden, Netherland
    • Year and Date
      2010-01-18
    • Related Report
      2008 Annual Research Report
  • [Presentation] Medical and Biological Applications Using Micro- and Nano-Devices2010

    • Author(s)
      Manabu Tokeshi
    • Organizer
      Swedish-Japanese Collaborative Workshop
    • Place of Presentation
      Uppsala, Sweden
    • Year and Date
      2010-01-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] Medical and Biological Applications Using Micro-and Nano-Devices2010

    • Author(s)
      渡慶次学
    • Organizer
      Swedish-Japanese Collaborative Workshop
    • Place of Presentation
      Uppsala, Sweden
    • Year and Date
      2010-01-09
    • Related Report
      2008 Annual Research Report
  • [Presentation] 生体内細胞追跡のための脂肪組織由来幹細胞の量子ドットによる標識2009

    • Author(s)
      渡辺将生, 他9人
    • Organizer
      第19回日本MRS学術シンポジウム
    • Place of Presentation
      横浜市開港記念会館
    • Year and Date
      2009-12-09
    • Related Report
      2009 Annual Research Report 2008 Annual Research Report
  • [Presentation] マイクロデバイスによるバイオ応用研究と臨床診断2009

    • Author(s)
      渡慶次学
    • Organizer
      沖縄県理科・化学教育フォーラム
    • Place of Presentation
      琉球大学
    • Year and Date
      2009-11-13
    • Related Report
      2009 Annual Research Report 2008 Annual Research Report
  • [Presentation] リアルタイムPCRチップの開発(2)2009

    • Author(s)
      内藤豊裕, 他7人
    • Organizer
      第20回化学とマイクロ・ナノシステム研究会
    • Place of Presentation
      石川四高記念文化交流館
    • Year and Date
      2009-11-09
    • Related Report
      2009 Annual Research Report 2008 Annual Research Report
  • [Presentation] Development of On-Chip Real Time PCR (2)2009

    • Author(s)
      Toyohiro Naito, 他7人
    • Organizer
      International Symposium on Microchemistry and Microsystems 2009
    • Place of Presentation
      石川四高記念文化交流館
    • Year and Date
      2009-11-07
    • Related Report
      2009 Annual Research Report
  • [Presentation] Development of On-Chip Real Time PCR(2)2009

    • Author(s)
      内藤豊裕, 他7人
    • Organizer
      International Symposium on Microchemistry and Microsystems 2009
    • Place of Presentation
      石川四高記念文化交流館
    • Year and Date
      2009-11-07
    • Related Report
      2008 Annual Research Report
  • [Presentation] Quantum Dot Labeing of Adipose Tissue-Derived Stem Cell for In Vivo Imaging2009

    • Author(s)
      Yukimasa Kagami, 他7人
    • Organizer
      3rd International Symposium on Nanomedicine Asia Core Symposium-Molecular Imaging and System Biology
    • Place of Presentation
      岡崎コンファレンスセンター
    • Year and Date
      2009-11-05
    • Related Report
      2009 Annual Research Report
  • [Presentation] Quantum Dot Based In Vivo Imaging of Transplanted Stem Cell Therapy2009

    • Author(s)
      Masaki Watanabe, 他9人
    • Organizer
      3rd International Symposium on Nanomedicine Asia Core Symposium-Molecular Imaging and System Biology
    • Place of Presentation
      岡崎コンファレンスセンター
    • Year and Date
      2009-11-05
    • Related Report
      2009 Annual Research Report 2008 Annual Research Report
  • [Presentation] Quantum Dot Labeing of Adipose Tissue-Derived Stem Cell for In Vivo2009

    • Author(s)
      鏡味幸真, 他4人
    • Organizer
      3rd International Symposium on Nanomedicine Asia Core Symposium-Molecular Imaging and System Biology
    • Place of Presentation
      岡崎コンファレンスセンター
    • Year and Date
      2009-11-05
    • Related Report
      2008 Annual Research Report
  • [Presentation] Easy Cell Assay Device Using Two Microchannels2009

    • Author(s)
      Kanako Sugiura, 他4人
    • Organizer
      13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS2009)
    • Place of Presentation
      Jeju, Korea
    • Year and Date
      2009-11-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] Easy Cell Assay Device Using Two Microchannels2009

    • Author(s)
      杉浦佳奈子, 他4人
    • Organizer
      13th International Conference on Miniaturized Systems for Chemistry and Life Sciences(μTAS2009)
    • Place of Presentation
      Jeju, Korea
    • Year and Date
      2009-11-03
    • Related Report
      2008 Annual Research Report
  • [Presentation] On-Chip Real Time PCR for Genetic Tug-of-War (g-TOW) Experiment2009

    • Author(s)
      Toyohiro Naito, 他6人
    • Organizer
      13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS2009)
    • Place of Presentation
      Jeju, Korea
    • Year and Date
      2009-11-02
    • Related Report
      2009 Annual Research Report
  • [Presentation] On-Chip Real Time PCR for Genetic Tug-of-War(g-TOW)Experiment2009

    • Author(s)
      内藤豊裕, 他6人
    • Organizer
      13th International Conference on Miniaturized Systems for Chemistry and Life Sciences(μTAS2009)
    • Place of Presentation
      Jeju, Korea
    • Year and Date
      2009-11-02
    • Related Report
      2008 Annual Research Report
  • [Presentation] 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences(μTAS 2009)2009

    • Author(s)
      Kanoko Sugiura, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba
    • Place of Presentation
      Jeju, Korea.
    • Year and Date
      2009-10-03
    • Related Report
      2010 Final Research Report
  • [Presentation] マイクロ・ナノデバイスのバイオ・臨床応用2009

    • Author(s)
      渡慶次学
    • Organizer
      東京大学大学院工学系研究科応用化学専攻談話会「応用化学の新しい潮流-分析化学」
    • Place of Presentation
      東京大学
    • Year and Date
      2009-10-03
    • Related Report
      2009 Annual Research Report 2008 Annual Research Report
  • [Presentation] 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences(μTAS 2009)2009

    • Author(s)
      Toyohiro Naito, Ai Yatsuhashi, Noritada Kaji, Taeko Ando, Kazuo Sato, Hisao Moriya, Hiroaki Kitano, Yukihiro Okamoto, Manabu Tokeshi, Yoshinobu Baba
    • Place of Presentation
      Jeiu, Korea.
    • Year and Date
      2009-10-02
    • Related Report
      2010 Final Research Report
  • [Presentation] マイクロ流路内での拡散を利用した細胞分析デバイスの開発2009

    • Author(s)
      杉浦佳奈子, 他4人
    • Organizer
      名古屋大学若手女性研究者サイエンスフォーラム
    • Place of Presentation
      名古屋大学
    • Year and Date
      2009-08-04
    • Related Report
      2009 Annual Research Report 2008 Annual Research Report
  • [Presentation]2009

    • Author(s)
      八橋愛、内藤豊裕、加地範匡、岡本行宏、渡慶次学、馬場嘉信
    • Organizer
      第19回化学とマイクロ・ナノシステム研究会
    • Place of Presentation
      広島大学
    • Year and Date
      2009-05-29
    • Related Report
      2010 Final Research Report
  • [Presentation] リアルタイムPCRチップの開発2009

    • Author(s)
      八橋愛, 他5人
    • Organizer
      第19回化学とマイクロ・ナノシステム研究会
    • Place of Presentation
      広島大学東広島キャンパス
    • Year and Date
      2009-05-29
    • Related Report
      2009 Annual Research Report 2008 Annual Research Report
  • [Presentation] 細胞パターンニングと濃度勾配を利用した細胞アッセイシステムの開発2009

    • Author(s)
      杉浦佳奈子, 他4人
    • Organizer
      第19回化学とマイクロ・ナノシステム研究会
    • Place of Presentation
      広島大学東広島キャンパス
    • Year and Date
      2009-05-28
    • Related Report
      2009 Annual Research Report 2008 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.apehem.nagoya-u.ac.jp/III-2/baba-ken/

    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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