Basic research of high-throughput-technology which adds a small amount of liquid reagents using impact force generated with femtosecond laser
| Project/Area Number |
20310075
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | Tohoku Fukushi University (2010-2011)
Nara Institute of Science and Technology (2008-2009) |
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Principal Investigator |
OKANO Kazunori 東北福祉大学, 感性福祉研究所, 研究員 (00443250)
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| Co-Investigator(Kenkyū-buntansha) |
MASUHARA Hiroshi 奈良先端科学技術大学院大学, 特任教授 (60029551)
HOSOKAWA Yoichiroh 奈良先端科学技術大学院大学, 特任准教授 (20448088)
UWADA Takayuki 奈良先端科学技術大学院大学, 研究員 (30455448)
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| Project Period (FY) |
2008 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2010: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2009: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2008: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
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| Keywords | マイクロバイオシステム / フェムト秒レーザー / 極微量液中試薬添加 / レーザー誘起応力波 / 細胞刺激 / 細胞アレイ / 液中レーザーリソグラフィー / システム細胞工学 / 液中試薬添加 / 液中表面改質 / 細胞間接着因子 / 細胞回路 |
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| Research Abstract |
We developed fundamental technologies by using a femtosecond laser(fs laser) on living-cell stimulation and manipulation. 1) Drags were delivered to limited area in culture medium by applying an fs laser-induced impact for bombing targeted cells with the drags. 2) The impact could release a targeted cell from a culture plate and delivered to a desired position. 3) Surface characteristic of the culture platform was changed from cytophobic to cytophilic when the fs laser was focused to the platform coated with cytophobic polymer. In conclusion, this fs-laser process in aqueous solution achieved the limited cell stimulation and the multiple cell arrangement.
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Report
(4 results)
Research Products
(71 results)
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[Remarks] (5) Y. Yasuyo, Y. Hosokawa, K. Okano, M. Matsubara, H. Masuhara, Fluorescence visualization of cell detachment process from a substrate by femtosecond laser "tsunami", The 1st NCTU-NAIST Workshop on "Molecular/Nano Sci.", Nov. 11-13, 2009, National Chiao-Tung Univ.(Hsinchu, Taiwan)
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[Remarks] (6) A. Matsui, Y. Maezawa, Y. Hosokawa, K. Okano, M. Matsui, H. Masuhara, Fabrication of cell-array platform by femtosecond laser process in culture medium, The 1st NCTU-NAIST Workshop on "Molecular/Nano Sci." Nov. 11-12, 2009, National Chiao-Tung Univ.(Hsinchu, Taiwan)
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[Remarks] (7) Y. Maezawa, Y. Hosokawa, K. Okano, M. Matsubara, H. Masuhara, Individual sorting of animal cells by femtosecond laser induced-stress wave, The 8th Gist/NIST joint symposium on advanced materials, Nov. 26-27, 2008, Nara Inst. Sci. Technol.(Ikoma, Japan
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