Strategies to search functional RNAs using yeast as a model system

• Project/Area Number: 20310114
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 基礎ゲノム科学
• Research Institution: The University of Tokyo
• Principal Investigator: ITO Takashi 東京大学, 大学院・理学系研究科, 教授 (90201326)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2010: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2009: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2008: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
• Keywords: 非コードRNA / 次世代シーケンシング / 人工染色体 / ヌクレオソーム / RNA-PET / 転写ノイズ / 出芽酵母 / クロマチン / ノイズ / 転写 / ロバストネス

Research Abstract

It is well documented that eukaryotic genome is pervasively transcribed to generate numerous novel non-coding RNAs, but the significance of such transcription remains controversial. We found that yeast cell transcribes a bacterial genome maintained as an artificial chromosome, which should have no biological function, as efficiently as its natural chromosomes. We also showed that limiting transcriptional capacity of yeast leads to a preferential decrease in the level of novel non-coding RNA species identified in recent studies. These results do not contradict with the idea that pervasive transcription is largely functionless.

Research Products

• [Journal Article] Translation initiation : a regulatory rolefor poly(A) tracts in front of the AUG codon in Saccharomyces cerevisiae (2011)
  • Author(s): Xia, X., MacKay, V., Yao, X., Wu, J., Miura, F., Ito, T., Morris, D. R.
  • Journal Title: Genetics Volume: 189 Issue: 2 Pages: 469-478
  • DOI: 10.1534/genetics.111.132068
  • Peer Reviewed
• [Journal Article] Translation initiation : a regulatory role for poly (A) tracts in front of the AUG codon in Saccbaromyces cerevisiae (2011)
  • Author(s): Xia, X., MacKay, V, Yao, X., Wu, J., Miura, F., Ito, T., Morris, D.R.
  • Journal Title: Genetics Volume: 189 Pages: 469-478
  • Peer Reviewed
• [Journal Article] Unexpected complexity of the budding yeasttranscriptome (2008)
  • Author(s): Ito, T., Miura, F., Onda, M.
  • Journal Title: IUBMB Life Volume: 60 Issue: 12 Pages: 775-781
  • DOI: 10.1002/iub.121
  • Peer Reviewed
• [Journal Article] Absolute quantification of budding yeasttranscriptome by means of competitive PCRbetween genomic and complementary DNAs (2008)
  • Author(s): Miura, F., Kawaguchi, N., Yoshida, M., Uematsu, C., Kito, K., Sakaki, Y., Ito, T.
  • Journal Title: BMC Genomics Volume: 9 Issue: 1 Pages: 574-574
  • DOI: 10.1186/1471-2164-9-574
  • Peer Reviewed
• [Journal Article] Absolute quantification of budding yeast transcriptome by means of competitive PCR between genomic and complementary DNAs. (2008)
  • Author(s): Miura, F., Kawaguchi, N., Yoshida, M., Uematsu, C., Kito, K., Sakaki, Y., Ito, T.
  • Journal Title: BMC Genomics 9 Pages: 574-574
  • Peer Reviewed
• [Journal Article] Unexpected complexity of the budding yeast transcriptome. (2008)
  • Author(s): Ito, T., Miura, F., Onda, M.
  • Journal Title: IUBMB Life 60 Pages: 775-781
  • Peer Reviewed
• [Presentation] 転写容量の制限によるトランスクリプトーム変動の解析 (2009)
  • Author(s): 前橋慶一、太田一寿、恩田美雪、伊藤隆司
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜
• [Presentation] 転写容量の制限によるトランスクリプトーム変動の解析 (2009)
  • Author(s): 前橋慶一、太田一寿、恩田美雪、伊藤隆司
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: 横浜パシフィコ
• [Presentation] Unexpected complexity ofthe budding yeast transcriptome (2008)
  • Author(s): Takashi Ito
  • Organizer: ASBMB2008
  • Place of Presentation: San Diego, USA
  • Year and Date: 2008-04-06
• [Presentation] Unexpected complexity of the budding yeast transcriptome (2008)
  • Author(s): Takashi Ito
  • Organizer: ASBMB2008
  • Place of Presentation: San Diego, USA
  • Year and Date: 2008-04-06