| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2010: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2008: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Research Abstract |
We have developed methods to comprehensively quantify cellular protein turnover, including synthesis rate, degradation rate and translation efficiency of corresponding mRNA. Using these methods we quantified turnover of more than 1500 protein in E. coli, and found that most of the protein, excluding about 5% of unstable protein, were quite stable without any degradation. Upon transition from anaerobic to aerobic growth condition, levels of metabolic enzymes changes dramatically. However these changes are brought mostly by changes in synthesis rate but no apparent degradation of unwanted enzymes in central carbon metabolism were observed.
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